Restriction map for
pF1KSDA1848
(Circular) (Six-base) MAPSORT of: pF1KSDA1848.seq Check: 3171 from: 1 to: 4349
pF1KSDA1848 4349 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 893 893
Size: 4349
AccI GT'mk_AC
Cuts at: 1244 1387 1244
Size: 143 4206
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 2174 2606 3353 3621 673
Size: 1501 432 747 268 1401
Fragments arranged by size:
1501 1401 747 432 268
AflIII A'CryG_T
Cuts at: 685 1701 2805 3782 685
Size: 1016 1104 977 1252
Fragments arranged by size:
1252 1104 1016 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AleI CACnn'nnGTG
Cuts at: 874 1006 874
Size: 132 4217
AlwNI CAG_nnn'CTG
Cuts at: 487 1654 3221 487
Size: 1167 1567 1615
Fragments arranged by size:
1615 1567 1167
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 202 1353 3702 3958 202
Size: 1151 2349 256 593
Fragments arranged by size:
2349 1151 593 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 176 257 335 1172 1369 176
Size: 81 78 837 197 3156
Fragments arranged by size:
3156 837 197 81 78
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 702 985 1000 1012 1083 1365 1779 2027 2062
Size: 283 15 12 71 282 414 248 35
Cuts at: 2062 702
Size: 2989
Fragments arranged by size:
2989 414 283 282 248 71 35 15
12
BanII G_rGCy'C
Cuts at: 800 1025 1235 1363 2393 800
Size: 225 210 128 1030 2756
Fragments arranged by size:
2756 1030 225 210 128
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbsI GAAGACnn'nnnn_
Cuts at: 226 469 589 226
Size: 243 120 3986
Fragments arranged by size:
3986 243 120
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 470 1869 470
Size: 1399 2950
BglI GCCn_nnn'nGGC
Cuts at: 1102 1222 1256 1102
Size: 120 34 4195
Fragments arranged by size:
4195 120 34
BglII A'GATC_T
Cuts at: 290 1864 3901 290
Size: 1574 2037 738
Fragments arranged by size:
2037 1574 738
BlpI GC'TnA_GC
Cuts at: 1333 1461 1333
Size: 128 4221
Bme1580I G_kGCm'C
Cuts at: 336 1015 1088 1974 2067 3123 336
Size: 679 73 886 93 1056 1562
Fragments arranged by size:
1562 1056 886 679 93 73
BmrI ACTGGGnnnn_n'
Cuts at: 323 508 1561 1642 1977 4121 323
Size: 185 1053 81 335 2144 551
Fragments arranged by size:
2144 1053 551 335 185 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1044 2716 1044
Size: 1672 2677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 874 589
Size: 285 4064
Bpu10I CC'TnA_GC
Cuts at: 1039 1039
Size: 4349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 366 1525 2404 2896 3194 315
Size: 51 1159 879 492 298 1470
Fragments arranged by size:
1470 1159 879 492 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 686 876 1341 1702 686
Size: 190 465 361 3333
Fragments arranged by size:
3333 465 361 190
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 151 621 1780 2028 2730 4226 151
Size: 470 1159 248 702 1496 274
Fragments arranged by size:
1496 1159 702 470 274 248
BsaWI w'CCGG_w
Cuts at: 1538 2059 2737 3011 3158 3569 1538
Size: 521 678 274 147 411 2318
Fragments arranged by size:
2318 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 337 367 517 547 686 716 337
Size: 30 150 30 139 30 3970
Fragments arranged by size:
3970 150 139 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 358 598 211
Size: 147 240 3962
Fragments arranged by size:
3962 240 147
BseYI C'CCAG_C
Cuts at: 438 969 1774 2532 3109 3605 438
Size: 531 805 758 577 496 1182
Fragments arranged by size:
1182 805 758 577 531 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 205
Size: 4349
BsiEI CG_ry'CG
Cuts at: 104 1891 1937 3145 104
Size: 1787 46 1208 1308
Fragments arranged by size:
1787 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 800 1181 1235 1363 2141 2331 3123 800
Size: 381 54 128 778 190 792 2026
Fragments arranged by size:
2026 792 778 381 190 128 54
BsmBI CGTCTCn'nnnn_
Cuts at: 850 850
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 336 800 1015 1025 1088 1181 1235 1363 1974
Size: 464 215 10 63 93 54 128 611
Cuts at: 1974 2067 2141 2331 2393 3123 336
Size: 93 74 190 62 730 1562
Fragments arranged by size:
1562 730 611 464 215 190 128 93
93 74 63 62 54 10
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 893 1399 1613 1915 2296 893
Size: 506 214 302 381 2946
Fragments arranged by size:
2946 506 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2641 2695 4179 4344 50
Size: 2591 54 1484 165 55
Fragments arranged by size:
2591 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2261 2261
Size: 4349
BsrFI r'CCGG_y
Cuts at: 617 824 2347 2528 2737 617
Size: 207 1523 181 209 2229
Fragments arranged by size:
2229 1523 209 207 181
BssSI C'ACGA_G
Cuts at: 898 2620 2978 898
Size: 1722 358 2269
Fragments arranged by size:
2269 1722 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 110 2710 110
Size: 2600 1749
BstEII G'GTnAC_C
Cuts at: 387 387
Size: 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 438 438
Size: 4349
BstYI r'GATC_y
Cuts at: 1 290 1374 1841 1864 2199 2445 3457 3543
Size: 289 1084 467 23 335 246 1012 86
Cuts at: 3543 3555 3901 1
Size: 12 346 449
Fragments arranged by size:
1084 1012 467 449 346 335 289 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 506 125
Size: 381 3968
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2299 2480 2643 2299
Size: 181 163 4005
Fragments arranged by size:
4005 181 163
BtsI GCAGTG_nn'
Cuts at: 1058 1212 1592 1058
Size: 154 380 3815
Fragments arranged by size:
3815 380 154
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 373 2055 2913 373
Size: 1682 858 1809
Fragments arranged by size:
1809 1682 858
EaeI y'GGCC_r
Cuts at: 207 428 746 1067 1094 1934 2108 2499 2526
Size: 221 318 321 27 840 174 391 27
Cuts at: 2526 3871 207
Size: 1345 685
Fragments arranged by size:
1345 840 685 391 321 318 221 174
27 27
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 1130 2372 2582 1130
Size: 1242 210 2897
Fragments arranged by size:
2897 1242 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2557 2867 3013 4159 2557
Size: 310 146 1146 2747
Fragments arranged by size:
2747 1146 310 146
EcoICRI GAG'CTC
Cuts at: 798 1233 1361 798
Size: 435 128 3786
Fragments arranged by size:
3786 435 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 673 874 2174 2606 3353 3621 589
Size: 84 201 1300 432 747 268 1317
Fragments arranged by size:
1317 1300 747 432 268 201 84
EcoNI CCTnn'n_nnAGG
Cuts at: 1135 1135
Size: 4349
EcoO109I rG'GnC_Cy
Cuts at: 375 1160 1308 1488 375
Size: 785 148 180 3236
Fragments arranged by size:
3236 785 180 148
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FspI TGC'GCA
Cuts at: 559 2130 559
Size: 1571 2778
HaeII r_GCGC'y
Cuts at: 755 1783 2031 3053 4032 755
Size: 1028 248 1022 979 1072
Fragments arranged by size:
1072 1028 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 711 743 2191 2221 2223 2253 711
Size: 32 1448 30 2 30 2807
Fragments arranged by size:
2807 1448 32 30 30 2
HincII GTy'rAC
Cuts at: 1388 1388
Size: 4349
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 134 3020 3204 134
Size: 2886 184 1279
Fragments arranged by size:
2886 1279 184
MscI TGG'CCA
Cuts at: 209 1096 2110 209
Size: 887 1014 2448
Fragments arranged by size:
2448 1014 887
MslI CAynn'nnrTG
Cuts at: 874 1006 2338 2465 874
Size: 132 1332 127 2758
Fragments arranged by size:
2758 1332 132 127
MspA1I CmG'CkG
Cuts at: 508 969 1460 1774 2134 3147 3392 508
Size: 461 491 314 360 1013 245 1465
Fragments arranged by size:
1465 1013 491 461 360 314 245
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 125 125
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NspI r_CATG'y
Cuts at: 951 1402 1902 2809 951
Size: 451 500 907 2491
Fragments arranged by size:
2491 907 500 451
PasI CC'CwG_GG
Cuts at: 981 981
Size: 4349
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PflMI CCAn_nnn'nTGG
Cuts at: 278 951 278
Size: 673 3676
PfoI T'CCnGG_A
Cuts at: 566 566
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PmlI CAC'GTG
Cuts at: 686 876 686
Size: 190 4159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 375 1160 1308 375
Size: 785 148 3416
Fragments arranged by size:
3416 785 148
PstI C_TGCA'G
Cuts at: 320 672 1396 2081 320
Size: 352 724 685 2588
Fragments arranged by size:
2588 724 685 352
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 969 1774 2134 969
Size: 805 360 3184
Fragments arranged by size:
3184 805 360
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 800 1235 1363 800
Size: 435 128 3786
Fragments arranged by size:
3786 435 128
SacII CC_GC'GG
Cuts at: 509 509
Size: 4349
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SanDI GG'GwC_CC
Cuts at: 1160 1160
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 316 668 1245 1392 2077 3070 3261 33
Size: 283 352 577 147 685 993 191 1121
Fragments arranged by size:
1121 993 685 577 352 283 191 147
SfiI GGCCn_nnn'nGGCC
Cuts at: 1102 1102
Size: 4349
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 178 259 1371 178
Size: 81 1112 3156
Fragments arranged by size:
3156 1112 81
SmlI C'TyrA_G
Cuts at: 294 381 1504 2419 2911 3173 294
Size: 87 1123 915 492 262 1470
Fragments arranged by size:
1470 1123 915 492 262 87
SnaBI TAC'GTA
Cuts at: 1341 1341
Size: 4349
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
SrfI GCCC'GGGC
Cuts at: 259 259
Size: 4349
StuI AGG'CCT
Cuts at: 1120 1120
Size: 4349
StyI C'CwwG_G
Cuts at: 125 662 1483 1837 125
Size: 537 821 354 2637
Fragments arranged by size:
2637 821 537 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1068 1932 2065 2524 2733 1068
Size: 864 133 459 209 2684
Fragments arranged by size:
2684 864 459 209 133
TatI w'GTAC_w
Cuts at: 413 3864 413
Size: 3451 898
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 517 549 686 718 3866 3898 517
Size: 32 137 32 3148 32 968
Fragments arranged by size:
3148 968 137 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 336 1077 1844 336
Size: 741 767 2841
Fragments arranged by size:
2841 767 741
XmaI C'CCGG_G
Cuts at: 176 257 1369 176
Size: 81 1112 3156
Fragments arranged by size:
3156 1112 81
XmnI GAAnn'nnTTC
Cuts at: 248 248
Size: 4349
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspOMI PspXI
PsrI SexAI SgrAI SspI SwaI XhoI ZraI