Restriction map for
pF1KSDA1852
(Circular) (Six-base) MAPSORT of: pF1KSDA1852.seq Check: 6074 from: 1 to: 5513
pF1KSDA1852 5513 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 425 425
Size: 5513
AatII G_ACGT'C
Cuts at: 1348 1348
Size: 5513
AccI GT'mk_AC
Cuts at: 2551 2551
Size: 5513
Acc65I G'GTAC_C
Cuts at: 2529 2529
Size: 5513
AclI AA'CG_TT
Cuts at: 5353 5353
Size: 5513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1503 1839 2091 3338 3770 4517 4785 1503
Size: 336 252 1247 432 747 268 2231
Fragments arranged by size:
2231 1247 747 432 336 268 252
AflIII A'CryG_T
Cuts at: 2865 3969 4946 2865
Size: 1104 977 3432
Fragments arranged by size:
3432 1104 977
AgeI A'CCGG_T
Cuts at: 3901 3901
Size: 5513
AhdI GACnn_n'nnGTC
Cuts at: 410 410
Size: 5513
AleI CACnn'nnGTG
Cuts at: 2150 2150
Size: 5513
AlwNI CAG_nnn'CTG
Cuts at: 2818 4385 2818
Size: 1567 3946
ApaLI G'TGCA_C
Cuts at: 4283 4283
Size: 5513
ApoI r'AATT_y
Cuts at: 757 1052 1527 1553 2141 2367 2517 4866 5122
Size: 295 475 26 588 226 150 2349 256
Cuts at: 5122 757
Size: 1148
Fragments arranged by size:
2349 1148 588 475 295 256 226 150
26
AseI AT'TA_AT
Cuts at: 20 20
Size: 5513
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
AvaI C'yCGr_G
Cuts at: 2533 2533
Size: 5513
BamHI G'GATC_C
Cuts at: 2538 2538
Size: 5513
BanI G'GyrC_C
Cuts at: 157 2529 2943 3191 3226 157
Size: 2372 414 248 35 2444
Fragments arranged by size:
2444 2372 414 248 35
BanII G_rGCy'C
Cuts at: 216 284 1114 1363 1699 1867 2527 3557 216
Size: 68 830 249 336 168 660 1030 2172
Fragments arranged by size:
2172 1030 830 660 336 249 168 68
BbeI G_GCGC'C
Cuts at: 2947 3195 2947
Size: 248 5265
BbsI GAAGACnn'nnnn_
Cuts at: 711 711
Size: 5513
BciVI GTATCCnnnnn_n'
Cuts at: 239 3411 4172 5513 239
Size: 3172 761 1341 239
Fragments arranged by size:
3172 1341 761 239
BclI T'GATC_A
Cuts at: 1041 3033 1041
Size: 1992 3521
BfrBI ATG'CAT
Cuts at: 1725 2397 1725
Size: 672 4841
BglI GCCn_nnn'nGGC
Cuts at: 211 211
Size: 5513
BglII A'GATC_T
Cuts at: 3028 5065 3028
Size: 2037 3476
BlpI GC'TnA_GC
Cuts at: 1446 1950 2625 1446
Size: 504 675 4334
Fragments arranged by size:
4334 675 504
Bme1580I G_kGCm'C
Cuts at: 162 3138 3231 4287 162
Size: 2976 93 1056 1388
Fragments arranged by size:
2976 1388 1056 93
BmgBI CAC'GTC
Cuts at: 324 324
Size: 5513
BmrI ACTGGGnnnn_n'
Cuts at: 287 2725 2806 3141 5285 287
Size: 2438 81 335 2144 515
Fragments arranged by size:
2438 2144 515 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 474 836 1087 3880 474
Size: 362 251 2793 2107
Fragments arranged by size:
2793 2107 362 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 421 624 998 1377 1415 1713 1751 1835
Size: 144 203 374 379 38 298 38 84
Cuts at: 1835 1881 2087 2255 2339 277
Size: 46 206 168 84 3451
Fragments arranged by size:
3451 379 374 298 206 203 168 144
84 84 46 38 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2689 3568 4060 4358 2689
Size: 879 492 298 3844
Fragments arranged by size:
3844 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 397 5217 397
Size: 4820 693
BsaAI yAC'GTr
Cuts at: 243 2505 2866 243
Size: 2262 361 2890
Fragments arranged by size:
2890 2262 361
BsaBI GATnn'nnATC
Cuts at: 6 3027 5064 6
Size: 3021 2037 455
Fragments arranged by size:
3021 2037 455
BsaHI Gr'CG_yC
Cuts at: 1345 2944 3192 3894 5390 1345
Size: 1599 248 702 1496 1468
Fragments arranged by size:
1599 1496 1468 702 248
BsaWI w'CCGG_w
Cuts at: 2219 2702 3223 3901 4175 4322 4733 2219
Size: 483 521 678 274 147 411 2999
Fragments arranged by size:
2999 678 521 483 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 282 274
Size: 8 5505
BseYI C'CCAG_C
Cuts at: 285 561 2938 3696 4273 4769 285
Size: 276 2377 758 577 496 1029
Fragments arranged by size:
2377 1029 758 577 496 276
BsiEI CG_ry'CG
Cuts at: 104 3055 3101 4309 104
Size: 2951 46 1208 1308
Fragments arranged by size:
2951 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1363 1699 1867 2527 3305 3495 4287 1363
Size: 336 168 660 778 190 792 2589
Fragments arranged by size:
2589 792 778 660 336 190 168
BsmI GAATG_Cn'
Cuts at: 1093 1727 1936 2089 2399 1093
Size: 634 209 153 310 4207
Fragments arranged by size:
4207 634 310 209 153
Bsp1286I G_dGCh'C
Cuts at: 162 216 284 1114 1363 1699 1867 2527 3138
Size: 54 68 830 249 336 168 660 611
Cuts at: 3138 3231 3305 3495 3557 4287 162
Size: 93 74 190 62 730 1388
Fragments arranged by size:
1388 830 730 660 611 336 249 190
168 93 74 68 62 54
BspEI T'CCGG_A
Cuts at: 4733 4733
Size: 5513
BspHI T'CATG_A
Cuts at: 4689 4689
Size: 5513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 425 2563 2777 3079 3460 425
Size: 2138 214 302 381 2478
Fragments arranged by size:
2478 2138 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3805 3859 5343 5508 50
Size: 3755 54 1484 165 55
Fragments arranged by size:
3755 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2210 3425 2210
Size: 1215 4298
BsrFI r'CCGG_y
Cuts at: 3511 3692 3901 3511
Size: 181 209 5123
Fragments arranged by size:
5123 209 181
BsrGI T'GTAC_A
Cuts at: 1542 2298 1542
Size: 756 4757
BssSI C'ACGA_G
Cuts at: 3784 4142 3784
Size: 358 5155
BstAPI GCAn_nnn'nTGC
Cuts at: 2890 2890
Size: 5513
BstBI TT'CG_AA
Cuts at: 110 3874 110
Size: 3764 1749
BstEII G'GTnAC_C
Cuts at: 412 900 412
Size: 488 5025
BstYI r'GATC_y
Cuts at: 1 2185 2538 3005 3028 3363 3609 4621 4707
Size: 2184 353 467 23 335 246 1012 86
Cuts at: 4707 4719 5065 1
Size: 12 346 449
Fragments arranged by size:
2184 1012 467 449 353 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 449 449
Size: 5513
BtgI C'CryG_G
Cuts at: 125 142 199 329 484 594 125
Size: 17 57 130 155 110 5044
Fragments arranged by size:
5044 155 130 110 57 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3463 3644 3807 3463
Size: 181 163 5169
Fragments arranged by size:
5169 181 163
BtsI GCAGTG_nn'
Cuts at: 1307 2430 2756 1307
Size: 1123 326 4064
Fragments arranged by size:
4064 1123 326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 331 366 1320 1355 2076 2111 331
Size: 35 954 35 721 35 3733
Fragments arranged by size:
3733 954 721 35 35 35
DraI TTT'AAA
Cuts at: 1746 2512 1746
Size: 766 4747
DrdI GACnn_nn'nnGTC
Cuts at: 3219 4077 3219
Size: 858 4655
EaeI y'GGCC_r
Cuts at: 326 3098 3272 3663 3690 5035 326
Size: 2772 174 391 27 1345 804
Fragments arranged by size:
2772 1345 804 391 174 27
EagI C'GGCC_G
Cuts at: 3098 3098
Size: 5513
EarI CTCTTCn'nnn_
Cuts at: 341 473 2483 3536 3746 341
Size: 132 2010 1053 210 2108
Fragments arranged by size:
2108 2010 1053 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3721 4031 4177 5323 3721
Size: 310 146 1146 3911
Fragments arranged by size:
3911 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1361 1697 1865 2525 1361
Size: 336 168 660 4349
Fragments arranged by size:
4349 660 336 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 421 624 998 1377 1415 1503 1713 1751
Size: 144 203 374 379 38 88 210 38
Cuts at: 1751 1835 1839 1881 2087 2091 2255 2339 3338
Size: 84 4 42 206 4 164 84 999
Cuts at: 3338 3770 4517 4785 277
Size: 432 747 268 1005
Fragments arranged by size:
1005 999 747 432 379 374 268 210
206 203 164 144 88 84 84 42
38 38 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 259 259
Size: 5513
EcoO109I rG'GnC_Cy
Cuts at: 377 2652 377
Size: 2275 3238
EcoRI G'AATT_C
Cuts at: 757 1052 1527 1553 2141 2367 2517 757
Size: 295 475 26 588 226 150 3753
Fragments arranged by size:
3753 588 475 295 226 150 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 5513
FspI TGC'GCA
Cuts at: 3294 3294
Size: 5513
HaeII r_GCGC'y
Cuts at: 2947 3195 4217 5196 2947
Size: 248 1022 979 3264
Fragments arranged by size:
3264 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 396 428 472 504 1686 1718 1854 1886 2272
Size: 32 44 32 1182 32 136 32 386
Cuts at: 2272 2304 3355 3385 3387 3417 396
Size: 32 1051 30 2 30 2492
Fragments arranged by size:
2492 1182 1051 386 136 44 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 195 2552 195
Size: 2357 3156
HindIII A'AGCT_T
Cuts at: 1766 1766
Size: 5513
KasI G'GCGC_C
Cuts at: 2943 3191 2943
Size: 248 5265
KpnI G_GTAC'C
Cuts at: 2533 2533
Size: 5513
MluI A'CGCG_T
Cuts at: 4946 4946
Size: 5513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 446 587 844 1125 4184 4368 446
Size: 141 257 281 3059 184 1591
Fragments arranged by size:
3059 1591 281 257 184 141
MscI TGG'CCA
Cuts at: 3274 3274
Size: 5513
MslI CAynn'nnrTG
Cuts at: 1037 1830 2150 2250 3502 3629 1037
Size: 793 320 100 1252 127 2921
Fragments arranged by size:
2921 1252 793 320 127 100
MspA1I CmG'CkG
Cuts at: 190 360 1523 1691 1859 2624 2938 3298 4311
Size: 170 1163 168 168 765 314 360 1013
Cuts at: 4311 4556 190
Size: 245 1147
Fragments arranged by size:
1163 1147 1013 765 360 314 245 170
168 168
NaeI GCC'GGC
Cuts at: 3694 3694
Size: 5513
NarI GG'CG_CC
Cuts at: 2944 3192 2944
Size: 248 5265
NcoI C'CATG_G
Cuts at: 125 199 594 125
Size: 74 395 5044
Fragments arranged by size:
5044 395 74
NdeI CA'TA_TG
Cuts at: 1715 1715
Size: 5513
NgoMIV G'CCGG_C
Cuts at: 3692 3692
Size: 5513
NsiI A_TGCA'T
Cuts at: 1727 2399 1727
Size: 672 4841
NspI r_CATG'y
Cuts at: 506 856 2566 3066 3973 506
Size: 350 1710 500 907 2046
Fragments arranged by size:
2046 1710 907 500 350
PciI A'CATG_T
Cuts at: 3969 3969
Size: 5513
PmeI GTTT'AAAC
Cuts at: 2512 2512
Size: 5513
PmlI CAC'GTG
Cuts at: 243 243
Size: 5513
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 4652
Size: 32 5481
PpuMI rG'GwC_Cy
Cuts at: 377 377
Size: 5513
PstI C_TGCA'G
Cuts at: 1314 2560 3245 1314
Size: 1246 685 3582
Fragments arranged by size:
3582 1246 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5513
PvuII CAG'CTG
Cuts at: 190 360 1523 1691 1859 2938 3298 190
Size: 170 1163 168 168 1079 360 2405
Fragments arranged by size:
2405 1163 1079 360 170 168 168
RsrII CG'GwC_CG
Cuts at: 3708 3708
Size: 5513
SacI G_AGCT'C
Cuts at: 1363 1699 1867 2527 1363
Size: 336 168 660 4349
Fragments arranged by size:
4349 660 336 168
SalI G'TCGA_C
Cuts at: 2550 2550
Size: 5513
SapI GCTCTTCn'nnn_
Cuts at: 473 3536 3746 473
Size: 3063 210 2240
Fragments arranged by size:
3063 2240 210
SbfI CC_TGCA'GG
Cuts at: 2560 2560
Size: 5513
ScaI AGT'ACT
Cuts at: 737 5030 737
Size: 4293 1220
SfcI C'TryA_G
Cuts at: 33 1226 1310 2022 2196 2556 3241 4234 4425
Size: 1193 84 712 174 360 685 993 191
Cuts at: 4425 33
Size: 1121
Fragments arranged by size:
1193 1121 993 712 685 360 191 174
84
SfoI GGC'GCC
Cuts at: 2945 3193 2945
Size: 248 5265
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
SmaI CCC'GGG
Cuts at: 2535 2535
Size: 5513
SmlI C'TyrA_G
Cuts at: 2668 3583 4075 4337 2668
Size: 915 492 262 3844
Fragments arranged by size:
3844 915 492 262
SnaBI TAC'GTA
Cuts at: 2505 2505
Size: 5513
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5513
SphI G_CATG'C
Cuts at: 2566 3066 2566
Size: 500 5013
StyI C'CwwG_G
Cuts at: 125 199 594 631 1132 2647 3001 125
Size: 74 395 37 501 1515 354 2637
Fragments arranged by size:
2637 1515 501 395 354 74 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 355 519 1145 3096 3229 3688 3897 355
Size: 164 626 1951 133 459 209 1971
Fragments arranged by size:
1971 1951 626 459 209 164 133
TatI w'GTAC_w
Cuts at: 697 735 997 1542 1666 1834 2298 5028 697
Size: 38 262 545 124 168 464 2730 1182
Fragments arranged by size:
2730 1182 545 464 262 168 124 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5030 5062 5030
Size: 32 5481
Tth111I GACn'n_nGTC
Cuts at: 3310 3310
Size: 5513
XbaI T'CTAG_A
Cuts at: 2544 2544
Size: 5513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 206 3008 206
Size: 2802 2711
XmaI C'CCGG_G
Cuts at: 2533 2533
Size: 5513
XmnI GAAnn'nnTTC
Cuts at: 1600 1684 1809 1600
Size: 84 125 5304
Fragments arranged by size:
5304 125 84
ZraI GAC'GTC
Cuts at: 1346 1346
Size: 5513
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI
BssHII BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI PacI PasI PflMI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI