Restriction map for
pF1KSDA1857
(Circular) (Six-base) MAPSORT of: pF1KSDA1857.seq Check: 877 from: 1 to: 4727
pF1KSDA1857 4727 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 971 1537 971
Size: 566 4161
AccI GT'mk_AC
Cuts at: 1765 1765
Size: 4727
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 2552 2984 3731 3999 283
Size: 2269 432 747 268 1011
Fragments arranged by size:
2269 1011 747 432 268
AfeI AGC'GCT
Cuts at: 1546 1546
Size: 4727
AflIII A'CryG_T
Cuts at: 304 677 1487 2079 3183 4160 304
Size: 373 810 592 1104 977 871
Fragments arranged by size:
1104 977 871 810 592 373
AgeI A'CCGG_T
Cuts at: 1382 3115 1382
Size: 1733 2994
AleI CACnn'nnGTG
Cuts at: 919 1573 919
Size: 654 4073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 907 937 1549 907
Size: 30 612 4085
Fragments arranged by size:
4085 612 30
AlwNI CAG_nnn'CTG
Cuts at: 1509 2032 3599 1509
Size: 523 1567 2637
Fragments arranged by size:
2637 1567 523
ApaI G_GGCC'C
Cuts at: 220 672 1432 220
Size: 452 760 3515
Fragments arranged by size:
3515 760 452
ApaLI G'TGCA_C
Cuts at: 1375 3497 1375
Size: 2122 2605
ApoI r'AATT_y
Cuts at: 1089 1731 4080 4336 1089
Size: 642 2349 256 1480
Fragments arranged by size:
2349 1480 642 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 1642 1747 1642
Size: 105 4622
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 913 1466 1525 1743 2157 2405 2440 913
Size: 553 59 218 414 248 35 3200
Fragments arranged by size:
3200 553 414 248 218 59 35
BanII G_rGCy'C
Cuts at: 220 293 672 1432 1741 2771 220
Size: 73 379 760 309 1030 2176
Fragments arranged by size:
2176 1030 760 379 309 73
BbeI G_GCGC'C
Cuts at: 1470 2161 2409 1470
Size: 691 248 3788
Fragments arranged by size:
3788 691 248
BciVI GTATCCnnnnn_n'
Cuts at: 2625 3386 4727 2625
Size: 761 1341 2625
Fragments arranged by size:
2625 1341 761
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglI GCCn_nnn'nGGC
Cuts at: 667 1295 1418 1562 667
Size: 628 123 144 3832
Fragments arranged by size:
3832 628 144 123
BglII A'GATC_T
Cuts at: 2242 4279 2242
Size: 2037 2690
BlpI GC'TnA_GC
Cuts at: 1839 1839
Size: 4727
Bme1580I G_kGCm'C
Cuts at: 220 672 1379 1432 2352 2445 3501 220
Size: 452 707 53 920 93 1056 1446
Fragments arranged by size:
1446 1056 920 707 452 93 53
BmgBI CAC'GTC
Cuts at: 534 534
Size: 4727
BmrI ACTGGGnnnn_n'
Cuts at: 1939 2020 2355 4499 1939
Size: 81 335 2144 2167
Fragments arranged by size:
2167 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 738 3094 738
Size: 2356 2371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 598 672 856 480
Size: 118 74 184 4351
Fragments arranged by size:
4351 184 118 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1903 2782 3274 3572 838
Size: 1065 879 492 298 1993
Fragments arranged by size:
1993 1065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 305 1387 4431 305
Size: 1082 3044 601
Fragments arranged by size:
3044 1082 601
BsaAI yAC'GTr
Cuts at: 756 1719 2080 756
Size: 963 361 3403
Fragments arranged by size:
3403 963 361
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 371 1467 2158 2406 3108 4604 371
Size: 1096 691 248 702 1496 494
Fragments arranged by size:
1496 1096 702 691 494 248
BsaWI w'CCGG_w
Cuts at: 1382 1916 2437 3115 3389 3536 3947 1382
Size: 534 521 678 274 147 411 2162
Fragments arranged by size:
2162 678 534 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 943 973 997 1027 1183 1213 943
Size: 30 24 30 156 30 4457
Fragments arranged by size:
4457 156 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 439 730 436
Size: 3 291 4433
Fragments arranged by size:
4433 291 3
BseYI C'CCAG_C
Cuts at: 472 728 906 1293 1689 2152 2910 3487 3983
Size: 256 178 387 396 463 758 577 496
Cuts at: 3983 472
Size: 1216
Fragments arranged by size:
1216 758 577 496 463 396 387 256
178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 693 943 136
Size: 557 250 3920
Fragments arranged by size:
3920 557 250
BsiEI CG_ry'CG
Cuts at: 104 558 1383 1635 2269 2315 3523 104
Size: 454 825 252 634 46 1208 1308
Fragments arranged by size:
1308 1208 825 634 454 252 46
BsiHKAI G_wGCw'C
Cuts at: 707 1015 1052 1379 1741 2519 2709 3501 707
Size: 308 37 327 362 778 190 792 1933
Fragments arranged by size:
1933 792 778 362 327 308 190 37
BsmI GAATG_Cn'
Cuts at: 1192 1192
Size: 4727
Bsp1286I G_dGCh'C
Cuts at: 220 293 672 707 1015 1052 1379 1432 1741
Size: 73 379 35 308 37 327 53 309
Cuts at: 1741 2352 2445 2519 2709 2771 3501 220
Size: 611 93 74 190 62 730 1446
Fragments arranged by size:
1446 730 611 379 327 309 308 190
93 74 73 62 53 37 35
BspEI T'CCGG_A
Cuts at: 3947 3947
Size: 4727
BspHI T'CATG_A
Cuts at: 3903 3903
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 812 887 971 1004 1133 1156 1249 1537 1777
Size: 75 84 33 129 23 93 288 240
Cuts at: 1777 1991 2293 2674 812
Size: 214 302 381 2865
Fragments arranged by size:
2865 381 302 288 240 214 129 93
84 75 33 23
BsrBI CCG'CTC
Cuts at: 50 3019 3073 4557 4722 50
Size: 2969 54 1484 165 55
Fragments arranged by size:
2969 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2639 2639
Size: 4727
BsrFI r'CCGG_y
Cuts at: 557 793 1060 1114 1289 1382 1396 1570 2725
Size: 236 267 54 175 93 14 174 1155
Cuts at: 2725 2906 3115 557
Size: 181 209 2169
Fragments arranged by size:
2169 1155 267 236 209 181 175 174
93 54 14
BssHII G'CGCG_C
Cuts at: 692 1637 692
Size: 945 3782
BssSI C'ACGA_G
Cuts at: 2998 3356 2998
Size: 358 4369
BstAPI GCAn_nnn'nTGC
Cuts at: 702 2104 702
Size: 1402 3325
BstBI TT'CG_AA
Cuts at: 110 3088 110
Size: 2978 1749
BstEII G'GTnAC_C
Cuts at: 201 1236 201
Size: 1035 3692
BstXI CCAn_nnnn'nTGG
Cuts at: 480 570 480
Size: 90 4637
BstYI r'GATC_y
Cuts at: 1 1752 2219 2242 2577 2823 3835 3921 3933
Size: 1751 467 23 335 246 1012 86 12
Cuts at: 3933 4279 1
Size: 346 449
Fragments arranged by size:
1751 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 294 294
Size: 4727
BtgI C'CryG_G
Cuts at: 515 563 1137 515
Size: 48 574 4105
Fragments arranged by size:
4105 574 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 153 1042 1275 1284 1970 153
Size: 889 233 9 686 2910
Fragments arranged by size:
2910 889 686 233 9
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DraIII CAC_nnn'GTG
Cuts at: 702 702
Size: 4727
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3291 2433
Size: 858 3869
EaeI y'GGCC_r
Cuts at: 434 555 1697 2312 2486 2877 2904 4249 434
Size: 121 1142 615 174 391 27 1345 912
Fragments arranged by size:
1345 1142 912 615 391 174 121 27
EagI C'GGCC_G
Cuts at: 555 2312 555
Size: 1757 2970
EarI CTCTTCn'nnn_
Cuts at: 151 2750 2960 151
Size: 2599 210 1918
Fragments arranged by size:
2599 1918 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 648 2935 3245 3391 4537 648
Size: 2287 310 146 1146 838
Fragments arranged by size:
2287 1146 838 310 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 480 598 672 856 2552 2984 3731 3999
Size: 197 118 74 184 1696 432 747 268
Cuts at: 3999 283
Size: 1011
Fragments arranged by size:
1696 1011 747 432 268 197 184 118
74
EcoO109I rG'GnC_Cy
Cuts at: 216 217 429 849 1429 1866 216
Size: 1 212 420 580 437 3077
Fragments arranged by size:
3077 580 437 420 212 1
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 879 2746 879
Size: 1867 2860
FseI GG_CCGG'CC
Cuts at: 561 561
Size: 4727
FspI TGC'GCA
Cuts at: 808 883 2508 808
Size: 75 1625 3027
Fragments arranged by size:
3027 1625 75
HaeII r_GCGC'y
Cuts at: 145 845 907 1470 1548 2161 2409 3431 4410
Size: 700 62 563 78 613 248 1022 979
Cuts at: 4410 145
Size: 462
Fragments arranged by size:
1022 979 700 613 563 462 248 78
62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 699 2569 2599 2601 2631 667
Size: 32 1870 30 2 30 2763
Fragments arranged by size:
2763 1870 32 30 30 2
HincII GTy'rAC
Cuts at: 1766 1766
Size: 4727
KasI G'GCGC_C
Cuts at: 1466 2157 2405 1466
Size: 691 248 3788
Fragments arranged by size:
3788 691 248
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 986 1226 3398 3582 401
Size: 585 240 2172 184 1546
Fragments arranged by size:
2172 1546 585 240 184
MscI TGG'CCA
Cuts at: 436 2488 436
Size: 2052 2675
MslI CAynn'nnrTG
Cuts at: 568 919 1573 2716 2843 568
Size: 351 654 1143 127 2452
Fragments arranged by size:
2452 1143 654 351 127
MspA1I CmG'CkG
Cuts at: 480 927 1131 1162 1208 1250 1506 1595 1838
Size: 447 204 31 46 42 256 89 243
Cuts at: 1838 2152 2512 3525 3770 480
Size: 314 360 1013 245 1437
Fragments arranged by size:
1437 1013 447 360 314 256 245 243
204 89 46 42 31
NaeI GCC'GGC
Cuts at: 559 795 1116 1291 2908 559
Size: 236 321 175 1617 2378
Fragments arranged by size:
2378 1617 321 236 175
NarI GG'CG_CC
Cuts at: 1467 2158 2406 1467
Size: 691 248 3788
Fragments arranged by size:
3788 691 248
NcoI C'CATG_G
Cuts at: 1137 1137
Size: 4727
NgoMIV G'CCGG_C
Cuts at: 557 793 1114 1289 2906 557
Size: 236 321 175 1617 2378
Fragments arranged by size:
2378 1617 321 236 175
NspI r_CATG'y
Cuts at: 308 681 888 1003 1780 2280 3187 308
Size: 373 207 115 777 500 907 1848
Fragments arranged by size:
1848 907 777 500 373 207 115
PciI A'CATG_T
Cuts at: 304 677 3183 304
Size: 373 2506 1848
Fragments arranged by size:
2506 1848 373
PflMI CCAn_nnn'nTGG
Cuts at: 443 443
Size: 4727
PfoI T'CCnGG_A
Cuts at: 575 575
Size: 4727
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PmlI CAC'GTG
Cuts at: 756 756
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4695
PshAI GACnn'nnGTC
Cuts at: 1243 1619 1243
Size: 376 4351
PspOMI G'GGCC_C
Cuts at: 216 668 1428 216
Size: 452 760 3515
Fragments arranged by size:
3515 760 452
PstI C_TGCA'G
Cuts at: 1037 1167 1213 1538 1774 2459 1037
Size: 130 46 325 236 685 3305
Fragments arranged by size:
3305 685 325 236 130 46
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 1250 1506 2152 2512 1250
Size: 256 646 360 3465
Fragments arranged by size:
3465 646 360 256
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 151 2750 2960 151
Size: 2599 210 1918
Fragments arranged by size:
2599 1918 210
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 721 946 4244 721
Size: 225 3298 1204
Fragments arranged by size:
3298 1204 225
SfcI C'TryA_G
Cuts at: 33 1033 1163 1209 1534 1770 2455 3448 3639
Size: 1000 130 46 325 236 685 993 191
Cuts at: 3639 33
Size: 1121
Fragments arranged by size:
1121 1000 993 685 325 236 191 130
46
SfoI GGC'GCC
Cuts at: 1468 2159 2407 1468
Size: 691 248 3788
Fragments arranged by size:
3788 691 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SgrAI Cr'CCGG_yG
Cuts at: 1570 1570
Size: 4727
SmaI CCC'GGG
Cuts at: 1644 1749 1644
Size: 105 4622
SmlI C'TyrA_G
Cuts at: 853 1882 2797 3289 3551 853
Size: 1029 915 492 262 2029
Fragments arranged by size:
2029 1029 915 492 262
SnaBI TAC'GTA
Cuts at: 1719 1719
Size: 4727
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 888 1003 1780 2280 888
Size: 115 777 500 3335
Fragments arranged by size:
3335 777 500 115
StuI AGG'CCT
Cuts at: 646 646
Size: 4727
StyI C'CwwG_G
Cuts at: 248 845 1137 1861 2215 248
Size: 597 292 724 354 2760
Fragments arranged by size:
2760 724 597 354 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 189 541 2310 2443 2902 3111 189
Size: 352 1769 133 459 209 1805
Fragments arranged by size:
1805 1769 459 352 209 133
TatI w'GTAC_w
Cuts at: 719 944 4242 719
Size: 225 3298 1204
Fragments arranged by size:
3298 1204 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 4695
Tth111I GACn'n_nGTC
Cuts at: 271 2524 271
Size: 2253 2474
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 384 2222 384
Size: 1838 2889
XmaI C'CCGG_G
Cuts at: 1642 1747 1642
Size: 105 4622
XmnI GAAnn'nnTTC
Cuts at: 1090 1090
Size: 4727
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI
BstZ17I ClaI CspCI EcoNI EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PpuMI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SrfI SspI SwaI XhoI ZraI