Restriction map for
pF1KSDA1859
(Circular) (Six-base) MAPSORT of: pF1KSDA1859.seq Check: 7862 from: 1 to: 5360
pF1KSDA1859 5360 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 841 841
Size: 5360
AccI GT'mk_AC
Cuts at: 2398 2398
Size: 5360
Acc65I G'GTAC_C
Cuts at: 2376 2376
Size: 5360
AclI AA'CG_TT
Cuts at: 5200 5200
Size: 5360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 492 1465 1555 3185 3617 4364 4632 126
Size: 366 973 90 1630 432 747 268 854
Fragments arranged by size:
1630 973 854 747 432 366 268 90
AfeI AGC'GCT
Cuts at: 2051 2051
Size: 5360
AflIII A'CryG_T
Cuts at: 2712 3816 4793 2712
Size: 1104 977 3279
Fragments arranged by size:
3279 1104 977
AgeI A'CCGG_T
Cuts at: 3748 3748
Size: 5360
AlwNI CAG_nnn'CTG
Cuts at: 378 1054 1057 1108 2665 4232 378
Size: 676 3 51 1557 1567 1506
Fragments arranged by size:
1567 1557 1506 676 51 3
ApaI G_GGCC'C
Cuts at: 602 739 922 2016 2028 602
Size: 137 183 1094 12 3934
Fragments arranged by size:
3934 1094 183 137 12
ApaLI G'TGCA_C
Cuts at: 4130 4130
Size: 5360
ApoI r'AATT_y
Cuts at: 238 1843 2364 4713 4969 238
Size: 1605 521 2349 256 629
Fragments arranged by size:
2349 1605 629 521 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5360
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
AvaI C'yCGr_G
Cuts at: 476 670 1302 1928 2380 476
Size: 194 632 626 452 3456
Fragments arranged by size:
3456 632 626 452 194
BamHI G'GATC_C
Cuts at: 399 1526 2138 2336 2385 399
Size: 1127 612 198 49 3374
Fragments arranged by size:
3374 1127 612 198 49
BanI G'GyrC_C
Cuts at: 262 1739 2233 2317 2376 2790 3038 3073 262
Size: 1477 494 84 59 414 248 35 2549
Fragments arranged by size:
2549 1477 494 414 248 84 59 35
BanII G_rGCy'C
Cuts at: 344 602 739 922 1136 1275 1866 2016 2028
Size: 258 137 183 214 139 591 150 12
Cuts at: 2028 2093 2374 3404 344
Size: 65 281 1030 2300
Fragments arranged by size:
2300 1030 591 281 258 214 183 150
139 137 65 12
BbeI G_GCGC'C
Cuts at: 2321 2794 3042 2321
Size: 473 248 4639
Fragments arranged by size:
4639 473 248
BbsI GAAGACnn'nnnn_
Cuts at: 179 822 179
Size: 643 4717
BbvCI CC'TCA_GC
Cuts at: 131 1590 131
Size: 1459 3901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1987 2021 1987
Size: 34 5326
BciVI GTATCCnnnnn_n'
Cuts at: 555 3258 4019 5360 555
Size: 2703 761 1341 555
Fragments arranged by size:
2703 1341 761 555
BclI T'GATC_A
Cuts at: 2880 2880
Size: 5360
BglI GCCn_nnn'nGGC
Cuts at: 589 673 721 904 1072 1687 589
Size: 84 48 183 168 615 4262
Fragments arranged by size:
4262 615 183 168 84 48
BglII A'GATC_T
Cuts at: 2875 4912 2875
Size: 2037 3323
BlpI GC'TnA_GC
Cuts at: 1118 1130 2472 1118
Size: 12 1342 4006
Fragments arranged by size:
4006 1342 12
Bme1580I G_kGCm'C
Cuts at: 602 739 922 944 2016 2028 2985 3078 4134
Size: 137 183 22 1072 12 957 93 1056
Cuts at: 4134 602
Size: 1828
Fragments arranged by size:
1828 1072 1056 957 183 137 93 22
12
BmrI ACTGGGnnnn_n'
Cuts at: 917 2572 2653 2988 5132 917
Size: 1655 81 335 2144 1145
Fragments arranged by size:
2144 1655 1145 335 81
BmtI G_CTAG'C
Cuts at: 527 527
Size: 5360
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1235 3727 1235
Size: 2492 2868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1260 1260
Size: 5360
Bpu10I CC'TnA_GC
Cuts at: 131 593 608 1088 1590 131
Size: 462 15 480 502 3901
Fragments arranged by size:
3901 502 480 462 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2536 3415 3907 4205 1305
Size: 1231 879 492 298 2460
Fragments arranged by size:
2460 1231 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 555 879 1867 1916 5064 555
Size: 324 988 49 3148 851
Fragments arranged by size:
3148 988 851 324 49
BsaAI yAC'GTr
Cuts at: 1329 2210 2352 2713 1329
Size: 881 142 361 3976
Fragments arranged by size:
3976 881 361 142
BsaBI GATnn'nnATC
Cuts at: 6 1423 2035 2874 4911 6
Size: 1417 612 839 2037 455
Fragments arranged by size:
2037 1417 839 612 455
BsaHI Gr'CG_yC
Cuts at: 788 1655 2222 2318 2791 3039 3741 5237 788
Size: 867 567 96 473 248 702 1496 911
Fragments arranged by size:
1496 911 867 702 567 473 248 96
BsaWI w'CCGG_w
Cuts at: 402 2291 2549 3070 3748 4022 4169 4580 402
Size: 1889 258 521 678 274 147 411 1182
Fragments arranged by size:
1889 1182 678 521 411 274 258 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1219 1222 1578 1627 1219
Size: 3 356 49 4952
Fragments arranged by size:
4952 356 49 3
BseYI C'CCAG_C
Cuts at: 566 799 894 2069 2272 2785 3543 4120 4616
Size: 233 95 1175 203 513 758 577 496
Cuts at: 4616 566
Size: 1310
Fragments arranged by size:
1310 1175 758 577 513 496 233 203
95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1121 1560 1121
Size: 439 4921
BsiEI CG_ry'CG
Cuts at: 104 428 2902 2948 4156 104
Size: 324 2474 46 1208 1308
Fragments arranged by size:
2474 1308 1208 324 46
BsiHKAI G_wGCw'C
Cuts at: 2093 2374 3152 3342 4134 2093
Size: 281 778 190 792 3319
Fragments arranged by size:
3319 792 778 281 190
BsmBI CGTCTCn'nnnn_
Cuts at: 415 415
Size: 5360
Bsp1286I G_dGCh'C
Cuts at: 344 602 739 922 944 1136 1275 1866 2016
Size: 258 137 183 22 192 139 591 150
Cuts at: 2016 2028 2093 2374 2985 3078 3152 3342 3404
Size: 12 65 281 611 93 74 190 62
Cuts at: 3404 4134 344
Size: 730 1570
Fragments arranged by size:
1570 730 611 591 281 258 192 190
183 150 139 137 93 74 65 62
22 12
BspEI T'CCGG_A
Cuts at: 402 2291 4580 402
Size: 1889 2289 1182
Fragments arranged by size:
2289 1889 1182
BspHI T'CATG_A
Cuts at: 1664 4536 1664
Size: 2872 2488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 841 897 2410 2624 2926 3307 537
Size: 304 56 1513 214 302 381 2590
Fragments arranged by size:
2590 1513 381 304 302 214 56
BsrBI CCG'CTC
Cuts at: 50 3652 3706 5190 5355 50
Size: 3602 54 1484 165 55
Fragments arranged by size:
3602 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 388 3272 388
Size: 2884 2476
BsrFI r'CCGG_y
Cuts at: 794 846 3358 3539 3748 794
Size: 52 2512 181 209 2406
Fragments arranged by size:
2512 2406 209 181 52
BssHII G'CGCG_C
Cuts at: 1431 1431
Size: 5360
BssSI C'ACGA_G
Cuts at: 1077 3631 3989 1077
Size: 2554 358 2448
Fragments arranged by size:
2554 2448 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1054 1057 1108 2737 1054
Size: 3 51 1629 3677
Fragments arranged by size:
3677 1629 51 3
BstBI TT'CG_AA
Cuts at: 110 3721 110
Size: 3611 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 660 783 1179 1238 660
Size: 123 396 59 4782
Fragments arranged by size:
4782 396 123 59
BstYI r'GATC_y
Cuts at: 1 399 1418 1526 1814 2138 2336 2385 2852
Size: 398 1019 108 288 324 198 49 467
Cuts at: 2852 2875 3210 3456 4468 4554 4566 4912 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1019 1012 467 449 398 346 335 324
288 246 198 108 86 49 23 12
BtgI C'CryG_G
Cuts at: 125 1223 125
Size: 1098 4262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1887 2138 3310 3491 3654 1887
Size: 251 1172 181 163 3593
Fragments arranged by size:
3593 1172 251 181 163
BtsI GCAGTG_nn'
Cuts at: 1112 2235 2603 1112
Size: 1123 368 3869
Fragments arranged by size:
3869 1123 368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1967 2002 1967
Size: 35 5325
DraI TTT'AAA
Cuts at: 2359 2359
Size: 5360
DraIII CAC_nnn'GTG
Cuts at: 534 534
Size: 5360
DrdI GACnn_nn'nnGTC
Cuts at: 993 1167 2083 3066 3924 993
Size: 174 916 983 858 2429
Fragments arranged by size:
2429 983 916 858 174
EaeI y'GGCC_r
Cuts at: 425 536 1010 1073 1994 2945 3119 3510 3537
Size: 111 474 63 921 951 174 391 27
Cuts at: 3537 4882 425
Size: 1345 903
Fragments arranged by size:
1345 951 921 903 474 391 174 111
63 27
EagI C'GGCC_G
Cuts at: 425 2945 425
Size: 2520 2840
EarI CTCTTCn'nnn_
Cuts at: 203 3383 3593 203
Size: 3180 210 1970
Fragments arranged by size:
3180 1970 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3568 3878 4024 5170 3568
Size: 310 146 1146 3758
Fragments arranged by size:
3758 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2091 2372 2091
Size: 281 5079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 492 1260 1465 1555 3185 3617 4364 4632
Size: 366 768 205 90 1630 432 747 268
Cuts at: 4632 126
Size: 854
Fragments arranged by size:
1630 854 768 747 432 366 268 205
90
EcoNI CCTnn'n_nnAGG
Cuts at: 382 1140 1363 1699 2133 382
Size: 758 223 336 434 3609
Fragments arranged by size:
3609 758 434 336 223
EcoO109I rG'GnC_Cy
Cuts at: 598 735 736 918 919 952 1307 1854 1890
Size: 137 1 182 1 33 355 547 36
Cuts at: 1890 2012 2024 2499 598
Size: 122 12 475 3459
Fragments arranged by size:
3459 547 475 355 182 137 122 36
33 12 1 1
EcoRI G'AATT_C
Cuts at: 238 1843 2364 238
Size: 1605 521 3234
Fragments arranged by size:
3234 1605 521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5360
FspI TGC'GCA
Cuts at: 3141 3141
Size: 5360
HaeII r_GCGC'y
Cuts at: 749 1009 2053 2321 2794 3042 4064 5043 749
Size: 260 1044 268 473 248 1022 979 1066
Fragments arranged by size:
1066 1044 1022 979 473 268 260 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2207 2239 3202 3232 3234 3264 2207
Size: 32 963 30 2 30 4303
Fragments arranged by size:
4303 963 32 30 30 2
HincII GTy'rAC
Cuts at: 2399 2399
Size: 5360
HindIII A'AGCT_T
Cuts at: 138 306 138
Size: 168 5192
KasI G'GCGC_C
Cuts at: 2317 2790 3038 2317
Size: 473 248 4639
Fragments arranged by size:
4639 473 248
KpnI G_GTAC'C
Cuts at: 2380 2380
Size: 5360
MluI A'CGCG_T
Cuts at: 4793 4793
Size: 5360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 488 4031 4215 488
Size: 3543 184 1633
Fragments arranged by size:
3543 1633 184
MscI TGG'CCA
Cuts at: 538 1012 1075 3121 538
Size: 474 63 2046 2777
Fragments arranged by size:
2777 2046 474 63
MslI CAynn'nnrTG
Cuts at: 1181 1663 3349 3476 1181
Size: 482 1686 127 3065
Fragments arranged by size:
3065 1686 482 127
MspA1I CmG'CkG
Cuts at: 396 484 493 1054 1105 1225 2471 2785 3145
Size: 88 9 561 51 120 1246 314 360
Cuts at: 3145 4158 4403 396
Size: 1013 245 1353
Fragments arranged by size:
1353 1246 1013 561 360 314 245 120
88 51 9
NaeI GCC'GGC
Cuts at: 848 3541 848
Size: 2693 2667
NarI GG'CG_CC
Cuts at: 2318 2791 3039 2318
Size: 473 248 4639
Fragments arranged by size:
4639 473 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5360
NgoMIV G'CCGG_C
Cuts at: 846 3539 846
Size: 2693 2667
NheI G'CTAG_C
Cuts at: 523 523
Size: 5360
NspI r_CATG'y
Cuts at: 1443 2008 2413 2913 3820 1443
Size: 565 405 500 907 2983
Fragments arranged by size:
2983 907 565 500 405
PasI CC'CwG_GG
Cuts at: 344 1136 1729 344
Size: 792 593 3975
Fragments arranged by size:
3975 792 593
PciI A'CATG_T
Cuts at: 3816 3816
Size: 5360
PflMI CCAn_nnn'nTGG
Cuts at: 378 534 378
Size: 156 5204
PfoI T'CCnGG_A
Cuts at: 2332 2332
Size: 5360
PmeI GTTT'AAAC
Cuts at: 2359 2359
Size: 5360
PmlI CAC'GTG
Cuts at: 2210 2210
Size: 5360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 5328
PpuMI rG'GwC_Cy
Cuts at: 952 1307 1890 952
Size: 355 583 4422
Fragments arranged by size:
4422 583 355
PspOMI G'GGCC_C
Cuts at: 598 735 918 2012 2024 598
Size: 137 183 1094 12 3934
Fragments arranged by size:
3934 1094 183 137 12
PstI C_TGCA'G
Cuts at: 1367 2407 3092 1367
Size: 1040 685 3635
Fragments arranged by size:
3635 1040 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5360
PvuII CAG'CTG
Cuts at: 396 1054 1105 2785 3145 396
Size: 658 51 1680 360 2611
Fragments arranged by size:
2611 1680 658 360 51
RsrII CG'GwC_CG
Cuts at: 3555 3555
Size: 5360
SacI G_AGCT'C
Cuts at: 2093 2374 2093
Size: 281 5079
SacII CC_GC'GG
Cuts at: 1226 1226
Size: 5360
SalI G'TCGA_C
Cuts at: 2397 2397
Size: 5360
SapI GCTCTTCn'nnn_
Cuts at: 3383 3593 3383
Size: 210 5150
SbfI CC_TGCA'GG
Cuts at: 2407 2407
Size: 5360
ScaI AGT'ACT
Cuts at: 4877 4877
Size: 5360
SfcI C'TryA_G
Cuts at: 33 162 1363 2403 3088 4081 4272 33
Size: 129 1201 1040 685 993 191 1121
Fragments arranged by size:
1201 1121 1040 993 685 191 129
SfiI GGCCn_nnn'nGGCC
Cuts at: 1072 1072
Size: 5360
SfoI GGC'GCC
Cuts at: 2319 2792 3040 2319
Size: 473 248 4639
Fragments arranged by size:
4639 473 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
SmaI CCC'GGG
Cuts at: 1930 2382 1930
Size: 452 4908
SmlI C'TyrA_G
Cuts at: 1320 2515 3430 3922 4184 1320
Size: 1195 915 492 262 2496
Fragments arranged by size:
2496 1195 915 492 262
SnaBI TAC'GTA
Cuts at: 2352 2352
Size: 5360
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5360
SphI G_CATG'C
Cuts at: 2413 2913 2413
Size: 500 4860
StuI AGG'CCT
Cuts at: 320 320
Size: 5360
StyI C'CwwG_G
Cuts at: 125 1061 1602 1613 2494 2848 125
Size: 936 541 11 881 354 2637
Fragments arranged by size:
2637 936 881 541 354 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1014 1248 1757 2943 3076 3535 3744 1014
Size: 234 509 1186 133 459 209 2630
Fragments arranged by size:
2630 1186 509 459 234 209 133
TatI w'GTAC_w
Cuts at: 2171 4875 2171
Size: 2704 2656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4877 4909 4877
Size: 32 5328
Tth111I GACn'n_nGTC
Cuts at: 196 3157 196
Size: 2961 2399
XbaI T'CTAG_A
Cuts at: 2391 2391
Size: 5360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 952 1068 2855 952
Size: 116 1787 3457
Fragments arranged by size:
3457 1787 116
XmaI C'CCGG_G
Cuts at: 1928 2380 1928
Size: 452 4908
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BsaXI BsiWI BsmI BsrGI BstEII
BstZ17I Bsu36I ClaI EcoRV FseI FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SanDI SexAI SgrAI SrfI SspI SwaI XhoI
XmnI ZraI