Restriction map for
pF1KSDA1862
(Circular) (Six-base) MAPSORT of: pF1KSDA1862.seq Check: 3422 from: 1 to: 6227
pF1KSDA1862 6227 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 3265 3265
Size: 6227
Acc65I G'GTAC_C
Cuts at: 3243 3243
Size: 6227
AclI AA'CG_TT
Cuts at: 6067 6067
Size: 6227
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 973 1318 2052 2099 4052 4484 5231 5499
Size: 183 345 734 47 1953 432 747 268
Cuts at: 5499 790
Size: 1518
Fragments arranged by size:
1953 1518 747 734 432 345 268 183
47
AfeI AGC'GCT
Cuts at: 2585 2585
Size: 6227
AflIII A'CryG_T
Cuts at: 3579 4683 5660 3579
Size: 1104 977 4146
Fragments arranged by size:
4146 1104 977
AgeI A'CCGG_T
Cuts at: 4615 4615
Size: 6227
AhdI GACnn_n'nnGTC
Cuts at: 1380 1380
Size: 6227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 272 272
Size: 6227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1029 1061 1029
Size: 32 6195
AlwNI CAG_nnn'CTG
Cuts at: 208 939 1156 1201 1632 1733 2104 2252 2532
Size: 731 217 45 431 101 371 148 280
Cuts at: 2532 2925 2928 3532 5099 208
Size: 393 3 604 1567 1336
Fragments arranged by size:
1567 1336 731 604 431 393 371 280
217 148 101 45 3
ApaI G_GGCC'C
Cuts at: 794 1240 2410 3162 794
Size: 446 1170 752 3859
Fragments arranged by size:
3859 1170 752 446
ApaLI G'TGCA_C
Cuts at: 4997 4997
Size: 6227
ApoI r'AATT_y
Cuts at: 725 3231 5580 5836 725
Size: 2506 2349 256 1116
Fragments arranged by size:
2506 2349 1116 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6227
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6227
AvaI C'yCGr_G
Cuts at: 382 430 802 964 1502 1786 2061 2091 3198
Size: 48 372 162 538 284 275 30 1107
Cuts at: 3198 3247 382
Size: 49 3362
Fragments arranged by size:
3362 1107 538 372 284 275 162 49
48 30
BamHI G'GATC_C
Cuts at: 2283 3107 3252 2283
Size: 824 145 5258
Fragments arranged by size:
5258 824 145
BanI G'GyrC_C
Cuts at: 797 2645 3096 3243 3657 3905 3940 797
Size: 1848 451 147 414 248 35 3084
Fragments arranged by size:
3084 1848 451 414 248 147 35
BanII G_rGCy'C
Cuts at: 430 455 639 653 794 886 962 1147 1240
Size: 25 184 14 141 92 76 185 93
Cuts at: 1240 1727 2111 2246 2410 2718 2903 3004 3162
Size: 487 384 135 164 308 185 101 158
Cuts at: 3162 3241 4271 430
Size: 79 1030 2386
Fragments arranged by size:
2386 1030 487 384 308 185 185 184
164 158 141 135 101 93 92 79
76 25 14
BbeI G_GCGC'C
Cuts at: 2649 3661 3909 2649
Size: 1012 248 4967
Fragments arranged by size:
4967 1012 248
BbsI GAAGACnn'nnnn_
Cuts at: 907 2093 2231 2367 907
Size: 1186 138 136 4767
Fragments arranged by size:
4767 1186 138 136
BbvCI CC'TCA_GC
Cuts at: 1194 1692 1763 1194
Size: 498 71 5658
Fragments arranged by size:
5658 498 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 421 455 2244 2278 3120 3154 421
Size: 34 1789 34 842 34 3494
Fragments arranged by size:
3494 1789 842 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1054 4125 4886 6227 1054
Size: 3071 761 1341 1054
Fragments arranged by size:
3071 1341 1054 761
BclI T'GATC_A
Cuts at: 3747 3747
Size: 6227
BglI GCCn_nnn'nGGC
Cuts at: 1176 1680 2001 2654 1176
Size: 504 321 653 4749
Fragments arranged by size:
4749 653 504 321
BglII A'GATC_T
Cuts at: 3742 5779 3742
Size: 2037 4190
BlpI GC'TnA_GC
Cuts at: 167 475 3339 167
Size: 308 2864 3055
Fragments arranged by size:
3055 2864 308
Bme1580I G_kGCm'C
Cuts at: 794 847 1240 2410 2859 3162 3852 3945 5001
Size: 53 393 1170 449 303 690 93 1056
Cuts at: 5001 794
Size: 2020
Fragments arranged by size:
2020 1170 1056 690 449 393 303 93
53
BmgBI CAC'GTC
Cuts at: 848 2860 3078 848
Size: 2012 218 3997
Fragments arranged by size:
3997 2012 218
BmrI ACTGGGnnnn_n'
Cuts at: 980 1554 2417 3439 3520 3855 5999 980
Size: 574 863 1022 81 335 2144 1208
Fragments arranged by size:
2144 1208 1022 863 574 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 490 4594 490
Size: 4104 2123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1070 1223 1654 1836 1990 2040 2326 2365
Size: 154 153 431 182 154 50 286 39
Cuts at: 2365 2769 2820 2855 3148 916
Size: 404 51 35 293 3995
Fragments arranged by size:
3995 431 404 293 286 182 154 154
153 51 50 39 35
Bpu10I CC'TnA_GC
Cuts at: 1194 1692 1763 2105 2279 2414 2600 1194
Size: 498 71 342 174 135 186 4821
Fragments arranged by size:
4821 498 342 186 174 135 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1232 1248 1630 3403 4282 4774 5072 1232
Size: 16 382 1773 879 492 298 2387
Fragments arranged by size:
2387 1773 879 492 382 298 16
BsaI GGTCTCn'nnnn_
Cuts at: 156 1388 5931 156
Size: 1232 4543 452
Fragments arranged by size:
4543 1232 452
BsaAI yAC'GTr
Cuts at: 3219 3580 3219
Size: 361 5866
BsaBI GATnn'nnATC
Cuts at: 6 3741 5778 6
Size: 3735 2037 455
Fragments arranged by size:
3735 2037 455
BsaHI Gr'CG_yC
Cuts at: 2646 3658 3906 4608 6104 2646
Size: 1012 248 702 1496 2769
Fragments arranged by size:
2769 1496 1012 702 248
BsaWI w'CCGG_w
Cuts at: 2439 2454 3416 3937 4615 4889 5036 5447 2439
Size: 15 962 521 678 274 147 411 3219
Fragments arranged by size:
3219 962 678 521 411 274 147 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1062 1092 2957 2987 1062
Size: 30 1865 30 4302
Fragments arranged by size:
4302 1865 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1067 1515 1680 1728 2475 2965 3041 3161 1067
Size: 448 165 48 747 490 76 120 4133
Fragments arranged by size:
4133 747 490 448 165 120 76 48
BseYI C'CCAG_C
Cuts at: 652 673 1133 1228 1232 1331 1364 1483 2032
Size: 21 460 95 4 99 33 119 549
Cuts at: 2032 2038 2044 2146 2637 2711 2918 2941 3141
Size: 6 6 102 491 74 207 23 200
Cuts at: 3141 3652 4410 4987 5483 652
Size: 511 758 577 496 1396
Fragments arranged by size:
1396 758 577 549 511 496 491 460
207 200 119 102 99 95 74 33
23 21 6 6 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 340 1945 2288 2518 2948 3086 3172 286
Size: 54 1605 343 230 430 138 86 3341
Fragments arranged by size:
3341 1605 430 343 230 138 86 54
BsiEI CG_ry'CG
Cuts at: 104 3769 3815 5023 104
Size: 3665 46 1208 1308
Fragments arranged by size:
3665 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 962 2111 3191 3241 4019 4209 5001 962
Size: 1149 1080 50 778 190 792 2188
Fragments arranged by size:
2188 1149 1080 792 778 190 50
BsmI GAATG_Cn'
Cuts at: 1304 1304
Size: 6227
BsmBI CGTCTCn'nnnn_
Cuts at: 136 1789 136
Size: 1653 4574
Bsp1286I G_dGCh'C
Cuts at: 430 455 639 653 794 847 886 962 1147
Size: 25 184 14 141 53 39 76 185
Cuts at: 1147 1240 1727 2111 2246 2410 2718 2859 2903
Size: 93 487 384 135 164 308 141 44
Cuts at: 2903 3004 3162 3191 3241 3852 3945 4019 4209
Size: 101 158 29 50 611 93 74 190
Cuts at: 4209 4271 5001 430
Size: 62 730 1656
Fragments arranged by size:
1656 730 611 487 384 308 190 185
184 164 158 141 141 135 101 93
93 76 74 62 53 50 44 39
29 25 14
BspEI T'CCGG_A
Cuts at: 2439 2454 5447 2439
Size: 15 2993 3219
Fragments arranged by size:
3219 2993 15
BspHI T'CATG_A
Cuts at: 5403 5403
Size: 6227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1164 2260 2831 3277 3491 3793 4174 1164
Size: 1096 571 446 214 302 381 3217
Fragments arranged by size:
3217 1096 571 446 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2121 2848 3117 4519 4573 6057 6222 50
Size: 2071 727 269 1402 54 1484 165 55
Fragments arranged by size:
2071 1484 1402 727 269 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4139 4139
Size: 6227
BsrFI r'CCGG_y
Cuts at: 4225 4406 4615 4225
Size: 181 209 5837
Fragments arranged by size:
5837 209 181
BssSI C'ACGA_G
Cuts at: 537 2766 4498 4856 537
Size: 2229 1732 358 1908
Fragments arranged by size:
2229 1908 1732 358
BstAPI GCAn_nnn'nTGC
Cuts at: 300 2532 3064 3604 300
Size: 2232 532 540 2923
Fragments arranged by size:
2923 2232 540 532
BstBI TT'CG_AA
Cuts at: 110 4588 110
Size: 4478 1749
BstEII G'GTnAC_C
Cuts at: 2818 2818
Size: 6227
BstXI CCAn_nnnn'nTGG
Cuts at: 1034 1916 2343 2586 2659 1034
Size: 882 427 243 73 4602
Fragments arranged by size:
4602 882 427 243 73
BstYI r'GATC_y
Cuts at: 1 270 335 1988 2283 2783 3107 3252 3719
Size: 269 65 1653 295 500 324 145 467
Cuts at: 3719 3742 4077 4323 5335 5421 5433 5779 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1653 1012 500 467 449 346 335 324
295 269 246 145 86 65 23 12
Bsu36I CC'TnA_GG
Cuts at: 993 1241 1981 3087 993
Size: 248 740 1106 4133
Fragments arranged by size:
4133 1106 740 248
BtgI C'CryG_G
Cuts at: 607 2698 607
Size: 2091 4136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4177 4358 4521 4177
Size: 181 163 5883
Fragments arranged by size:
5883 181 163
BtsI GCAGTG_nn'
Cuts at: 902 1621 1962 2211 2904 3470 902
Size: 719 341 249 693 566 3659
Fragments arranged by size:
3659 719 693 566 341 249
DraI TTT'AAA
Cuts at: 3226 3226
Size: 6227
DraIII CAC_nnn'GTG
Cuts at: 1420 1420
Size: 6227
DrdI GACnn_nn'nnGTC
Cuts at: 3933 4791 3933
Size: 858 5369
EaeI y'GGCC_r
Cuts at: 515 2609 2793 3812 3986 4377 4404 5749 515
Size: 2094 184 1019 174 391 27 1345 993
Fragments arranged by size:
2094 1345 1019 993 391 184 174 27
EagI C'GGCC_G
Cuts at: 3812 3812
Size: 6227
EarI CTCTTCn'nnn_
Cuts at: 207 679 2919 4250 4460 207
Size: 472 2240 1331 210 1974
Fragments arranged by size:
2240 1974 1331 472 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4435 4745 4891 6037 4435
Size: 310 146 1146 4625
Fragments arranged by size:
4625 1146 310 146
EcoICRI GAG'CTC
Cuts at: 960 2109 3239 960
Size: 1149 1130 3948
Fragments arranged by size:
3948 1149 1130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 916 973 1070 1223 1318 1654 1836 1990
Size: 126 57 97 153 95 336 182 154
Cuts at: 1990 2040 2052 2099 2326 2365 2769 2820 2855
Size: 50 12 47 227 39 404 51 35
Cuts at: 2855 3148 4052 4484 5231 5499 790
Size: 293 904 432 747 268 1518
Fragments arranged by size:
1518 904 747 432 404 336 293 268
227 182 154 153 126 97 95 57
51 50 47 39 35 12
EcoNI CCTnn'n_nnAGG
Cuts at: 464 1038 1595 2524 2599 2748 464
Size: 574 557 929 75 149 3943
Fragments arranged by size:
3943 929 574 557 149 75
EcoO109I rG'GnC_Cy
Cuts at: 710 791 874 1054 1153 1218 1236 1237 1490
Size: 81 83 180 99 65 18 1 253
Cuts at: 1490 1548 2003 2029 2189 2406 2621 2656 2704
Size: 58 455 26 160 217 215 35 48
Cuts at: 2704 2761 3158 3159 3195 3366 710
Size: 57 397 1 36 171 3571
Fragments arranged by size:
3571 455 397 253 217 215 180 171
160 99 83 81 65 58 57 48
36 35 26 18 1 1
EcoRI G'AATT_C
Cuts at: 3231 3231
Size: 6227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4246 4246
Size: 6227
FspI TGC'GCA
Cuts at: 4008 4008
Size: 6227
HaeII r_GCGC'y
Cuts at: 371 1810 1979 2587 2649 3661 3909 4931 5910
Size: 1439 169 608 62 1012 248 1022 979
Cuts at: 5910 371
Size: 688
Fragments arranged by size:
1439 1022 1012 979 688 608 248 169
62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1062 1094 1366 1398 1809 1841 4069 4099 4101
Size: 32 272 32 411 32 2228 30 2
Cuts at: 4101 4131 1062
Size: 30 3158
Fragments arranged by size:
3158 2228 411 272 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3266 3266
Size: 6227
HindIII A'AGCT_T
Cuts at: 816 2675 816
Size: 1859 4368
KasI G'GCGC_C
Cuts at: 2645 3657 3905 2645
Size: 1012 248 4967
Fragments arranged by size:
4967 1012 248
KpnI G_GTAC'C
Cuts at: 3247 3247
Size: 6227
MluI A'CGCG_T
Cuts at: 5660 5660
Size: 6227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4898 5082 4898
Size: 184 6043
MscI TGG'CCA
Cuts at: 517 2611 2795 3988 517
Size: 2094 184 1193 2756
Fragments arranged by size:
2756 2094 1193 184
MslI CAynn'nnrTG
Cuts at: 239 4216 4343 239
Size: 3977 127 2123
Fragments arranged by size:
3977 2123 127
MspA1I CmG'CkG
Cuts at: 166 324 439 479 1133 1232 1275 1335 1856
Size: 158 115 40 654 99 43 60 521
Cuts at: 1856 1878 1959 2072 2150 2258 2304 2865 2925
Size: 22 81 113 78 108 46 561 60
Cuts at: 2925 2945 3338 3652 4012 5025 5270 166
Size: 20 393 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 654 561 521 393 360 314
245 158 115 113 108 99 81 78
60 60 46 43 40 22 20
NaeI GCC'GGC
Cuts at: 4408 4408
Size: 6227
NarI GG'CG_CC
Cuts at: 2646 3658 3906 2646
Size: 1012 248 4967
Fragments arranged by size:
4967 1012 248
NgoMIV G'CCGG_C
Cuts at: 4406 4406
Size: 6227
NspI r_CATG'y
Cuts at: 3280 3780 4687 3280
Size: 500 907 4820
Fragments arranged by size:
4820 907 500
PasI CC'CwG_GG
Cuts at: 276 639 890 1894 2318 2403 2659 276
Size: 363 251 1004 424 85 256 3844
Fragments arranged by size:
3844 1004 424 363 256 251 85
PciI A'CATG_T
Cuts at: 4683 4683
Size: 6227
PflMI CCAn_nnn'nTGG
Cuts at: 1733 2182 1733
Size: 449 5778
PfoI T'CCnGG_A
Cuts at: 1026 1590 2680 3164 1026
Size: 564 1090 484 4089
Fragments arranged by size:
4089 1090 564 484
PmeI GTTT'AAAC
Cuts at: 3226 3226
Size: 6227
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1427 1459 5366 5398 1427
Size: 32 3907 32 2256
Fragments arranged by size:
3907 2256 32 32
PpuMI rG'GwC_Cy
Cuts at: 710 1153 1218 1548 2189 2621 2704 2761 3195
Size: 443 65 330 641 432 83 57 434
Cuts at: 3195 710
Size: 3742
Fragments arranged by size:
3742 641 443 434 432 330 83 65
57
PshAI GACnn'nnGTC
Cuts at: 148 148
Size: 6227
PspOMI G'GGCC_C
Cuts at: 790 1236 2406 3158 790
Size: 446 1170 752 3859
Fragments arranged by size:
3859 1170 752 446
PspXI vC'TCGA_Gb
Cuts at: 2061 2061
Size: 6227
PstI C_TGCA'G
Cuts at: 1463 1628 2257 2495 2525 2812 2911 3041 3060
Size: 165 629 238 30 287 99 130 19
Cuts at: 3060 3274 3959 1463
Size: 214 685 3731
Fragments arranged by size:
3731 685 629 287 238 214 165 130
99 30 19
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6227
PvuII CAG'CTG
Cuts at: 166 324 439 479 1133 1232 1335 1856 2150
Size: 158 115 40 654 99 103 521 294
Cuts at: 2150 2304 2925 2945 3652 4012 166
Size: 154 621 20 707 360 2381
Fragments arranged by size:
2381 707 654 621 521 360 294 158
154 115 103 99 40 20
RsrII CG'GwC_CG
Cuts at: 4422 4422
Size: 6227
SacI G_AGCT'C
Cuts at: 962 2111 3241 962
Size: 1149 1130 3948
Fragments arranged by size:
3948 1149 1130
SalI G'TCGA_C
Cuts at: 3264 3264
Size: 6227
SanDI GG'GwC_CC
Cuts at: 1218 2189 2761 3195 1218
Size: 971 572 434 4250
Fragments arranged by size:
4250 971 572 434
SapI GCTCTTCn'nnn_
Cuts at: 207 679 2919 4250 4460 207
Size: 472 2240 1331 210 1974
Fragments arranged by size:
2240 1974 1331 472 210
SbfI CC_TGCA'GG
Cuts at: 3041 3274 3041
Size: 233 5994
ScaI AGT'ACT
Cuts at: 5744 5744
Size: 6227
SfcI C'TryA_G
Cuts at: 33 1015 1459 1624 2253 2491 2521 2808 2907
Size: 982 444 165 629 238 30 287 99
Cuts at: 2907 3037 3056 3270 3955 4948 5139 33
Size: 130 19 214 685 993 191 1121
Fragments arranged by size:
1121 993 982 685 629 444 287 238
214 191 165 130 99 30 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 1680 1680
Size: 6227
SfoI GGC'GCC
Cuts at: 2647 3659 3907 2647
Size: 1012 248 4967
Fragments arranged by size:
4967 1012 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6227
SmaI CCC'GGG
Cuts at: 3249 3249
Size: 6227
SmlI C'TyrA_G
Cuts at: 1247 1263 1645 2061 3382 4297 4789 5051 1247
Size: 16 382 416 1321 915 492 262 2423
Fragments arranged by size:
2423 1321 915 492 416 382 262 16
SnaBI TAC'GTA
Cuts at: 3219 3219
Size: 6227
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6227
SphI G_CATG'C
Cuts at: 3280 3780 3280
Size: 500 5727
StuI AGG'CCT
Cuts at: 470 755 1768 2342 2594 2772 470
Size: 285 1013 574 252 178 3925
Fragments arranged by size:
3925 1013 574 285 252 178
StyI C'CwwG_G
Cuts at: 2652 3122 3361 3715 2652
Size: 470 239 354 5164
Fragments arranged by size:
5164 470 354 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 679 919 1356 1725 2955 3810 3943 4402 4611
Size: 240 437 369 1230 855 133 459 209
Cuts at: 4611 679
Size: 2295
Fragments arranged by size:
2295 1230 855 459 437 369 240 209
133
TatI w'GTAC_w
Cuts at: 5742 5742
Size: 6227
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2957 2989 3160 3192 5744 5776 2957
Size: 32 171 32 2552 32 3408
Fragments arranged by size:
3408 2552 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 565 4024 565
Size: 3459 2768
XbaI T'CTAG_A
Cuts at: 3258 3258
Size: 6227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 867 1090 1491 3722 867
Size: 223 401 2231 3372
Fragments arranged by size:
3372 2231 401 223
XhoI C'TCGA_G
Cuts at: 2061 2061
Size: 6227
XmaI C'CCGG_G
Cuts at: 3247 3247
Size: 6227
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AscI AvrII BaeI BfrBI
BmtI BsiWI BsrGI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PsrI SacII SexAI
SgrAI SrfI SspI SwaI XmnI ZraI