Restriction map for
pF1KSDA1869
(Circular) (Six-base) MAPSORT of: pF1KSDA1869.seq Check: 4008 from: 1 to: 5927
pF1KSDA1869 5927 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2594 2594
Size: 5927
AatII G_ACGT'C
Cuts at: 2031 2831 2031
Size: 800 5127
AccI GT'mk_AC
Cuts at: 833 1661 2965 833
Size: 828 1304 3795
Fragments arranged by size:
3795 1304 828
Acc65I G'GTAC_C
Cuts at: 2943 2943
Size: 5927
AclI AA'CG_TT
Cuts at: 5767 5767
Size: 5927
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1498 2872 3752 4184 4931 5199 988
Size: 510 1374 880 432 747 268 1716
Fragments arranged by size:
1716 1374 880 747 510 432 268
AflIII A'CryG_T
Cuts at: 3279 4383 5360 3279
Size: 1104 977 3846
Fragments arranged by size:
3846 1104 977
AgeI A'CCGG_T
Cuts at: 4315 4315
Size: 5927
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1798 1830 1798
Size: 32 5895
AlwNI CAG_nnn'CTG
Cuts at: 1757 1987 3232 4799 1757
Size: 230 1245 1567 2885
Fragments arranged by size:
2885 1567 1245 230
ApaI G_GGCC'C
Cuts at: 746 1389 2110 2357 2703 2873 746
Size: 643 721 247 346 170 3800
Fragments arranged by size:
3800 721 643 346 247 170
ApaLI G'TGCA_C
Cuts at: 4697 4697
Size: 5927
ApoI r'AATT_y
Cuts at: 2931 5280 5536 2931
Size: 2349 256 3322
Fragments arranged by size:
3322 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5927
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5927
AvaI C'yCGr_G
Cuts at: 356 902 935 1280 2227 2398 2596 2720 2730
Size: 546 33 345 947 171 198 124 10
Cuts at: 2730 2761 2791 2947 356
Size: 31 30 156 3336
Fragments arranged by size:
3336 947 546 345 198 171 156 124
33 31 30 10
BamHI G'GATC_C
Cuts at: 1237 2952 1237
Size: 1715 4212
BanI G'GyrC_C
Cuts at: 411 1975 2943 3357 3605 3640 411
Size: 1564 968 414 248 35 2698
Fragments arranged by size:
2698 1564 968 414 248 35
BanII G_rGCy'C
Cuts at: 746 1389 1520 2060 2110 2117 2357 2483 2522
Size: 643 131 540 50 7 240 126 39
Cuts at: 2522 2703 2873 2941 3971 746
Size: 181 170 68 1030 2702
Fragments arranged by size:
2702 1030 643 540 240 181 170 131
126 68 50 39 7
BbeI G_GCGC'C
Cuts at: 1979 3361 3609 1979
Size: 1382 248 4297
Fragments arranged by size:
4297 1382 248
BbsI GAAGACnn'nnnn_
Cuts at: 1071 1071
Size: 5927
BbvCI CC'TCA_GC
Cuts at: 2578 2578
Size: 5927
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2025 2059 2025
Size: 34 5893
BciVI GTATCCnnnnn_n'
Cuts at: 3825 4586 5927 3825
Size: 761 1341 3825
Fragments arranged by size:
3825 1341 761
BclI T'GATC_A
Cuts at: 3447 3447
Size: 5927
BglI GCCn_nnn'nGGC
Cuts at: 1954 2580 1954
Size: 626 5301
BglII A'GATC_T
Cuts at: 3442 5479 3442
Size: 2037 3890
BlpI GC'TnA_GC
Cuts at: 3039 3039
Size: 5927
Bme1580I G_kGCm'C
Cuts at: 138 416 746 1389 1686 2110 2198 2342 2357
Size: 278 330 643 297 424 88 144 15
Cuts at: 2357 2594 2703 2873 3552 3645 4701 138
Size: 237 109 170 679 93 1056 1364
Fragments arranged by size:
1364 1056 679 643 424 330 297 278
237 170 144 109 93 88 15
BmgBI CAC'GTC
Cuts at: 2589 2589
Size: 5927
BmrI ACTGGGnnnn_n'
Cuts at: 642 1046 1859 2835 3139 3220 3555 5699 642
Size: 404 813 976 304 81 335 2144 870
Fragments arranged by size:
2144 976 870 813 404 335 304 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2586 4294 2586
Size: 1708 4219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 1627 1734 2446 2727 295
Size: 1332 107 712 281 3495
Fragments arranged by size:
3495 1332 712 281 107
Bpu10I CC'TnA_GC
Cuts at: 1514 2578 1514
Size: 1064 4863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3103 3982 4474 4772 3103
Size: 879 492 298 4258
Fragments arranged by size:
4258 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 818 858 1277 2004 5631 818
Size: 40 419 727 3627 1114
Fragments arranged by size:
3627 1114 727 419 40
BsaAI yAC'GTr
Cuts at: 2919 3280 2919
Size: 361 5566
BsaBI GATnn'nnATC
Cuts at: 6 1793 3441 5478 6
Size: 1787 1648 2037 455
Fragments arranged by size:
2037 1787 1648 455
BsaHI Gr'CG_yC
Cuts at: 1976 2028 2152 2660 2828 3358 3606 4308 5804
Size: 52 124 508 168 530 248 702 1496
Cuts at: 5804 1976
Size: 2099
Fragments arranged by size:
2099 1496 702 530 508 248 168 124
52
BsaWI w'CCGG_w
Cuts at: 335 2184 3116 3637 4315 4589 4736 5147 335
Size: 1849 932 521 678 274 147 411 1115
Fragments arranged by size:
1849 1115 932 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 355 385 815 845 355
Size: 30 430 30 5437
Fragments arranged by size:
5437 430 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1191 1263 1719 1930 2865 1191
Size: 72 456 211 935 4253
Fragments arranged by size:
4253 935 456 211 72
BseYI C'CCAG_C
Cuts at: 746 873 1801 1905 2545 2655 3352 4110 4687
Size: 127 928 104 640 110 697 758 577
Cuts at: 4687 5183 746
Size: 496 1490
Fragments arranged by size:
1490 928 758 697 640 577 496 127
110 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1265 1581 2167 2431 904
Size: 361 316 586 264 4400
Fragments arranged by size:
4400 586 361 316 264
BsiEI CG_ry'CG
Cuts at: 104 2904 3469 3515 4723 104
Size: 2800 565 46 1208 1308
Fragments arranged by size:
2800 1308 1208 565 46
BsiHKAI G_wGCw'C
Cuts at: 2941 3719 3909 4701 2941
Size: 778 190 792 4167
Fragments arranged by size:
4167 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 138 416 746 1389 1520 1686 2060 2110 2117
Size: 278 330 643 131 166 374 50 7
Cuts at: 2117 2198 2342 2357 2483 2522 2594 2703 2873
Size: 81 144 15 126 39 72 109 170
Cuts at: 2873 2941 3552 3645 3719 3909 3971 4701 138
Size: 68 611 93 74 190 62 730 1364
Fragments arranged by size:
1364 730 643 611 374 330 278 190
170 166 144 131 126 109 93 81
74 72 68 62 50 39 15 7
BspEI T'CCGG_A
Cuts at: 2184 5147 2184
Size: 2963 2964
BspHI T'CATG_A
Cuts at: 5103 5103
Size: 5927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1058 1907 2453 2594 2977 3191 3493 3874 1058
Size: 849 546 141 383 214 302 381 3111
Fragments arranged by size:
3111 849 546 383 381 302 214 141
BsrBI CCG'CTC
Cuts at: 50 761 1061 1922 2327 2647 4219 4273 5757
Size: 711 300 861 405 320 1572 54 1484
Cuts at: 5757 5922 50
Size: 165 55
Fragments arranged by size:
1572 1484 861 711 405 320 300 165
55 54
BsrDI GCAATG_nn'
Cuts at: 3839 3839
Size: 5927
BsrFI r'CCGG_y
Cuts at: 1676 2247 2786 3925 4106 4315 1676
Size: 571 539 1139 181 209 3288
Fragments arranged by size:
3288 1139 571 539 209 181
BssSI C'ACGA_G
Cuts at: 2560 4198 4556 2560
Size: 1638 358 3931
Fragments arranged by size:
3931 1638 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1966 3304 1966
Size: 1338 4589
BstBI TT'CG_AA
Cuts at: 110 4288 110
Size: 4178 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 260 1527 260
Size: 1267 4660
BstYI r'GATC_y
Cuts at: 1 1237 1774 2952 3419 3442 3777 4023 5035
Size: 1236 537 1178 467 23 335 246 1012
Cuts at: 5035 5121 5133 5479 1
Size: 86 12 346 449
Fragments arranged by size:
1236 1178 1012 537 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 1817 2097 2408 1817
Size: 280 311 5336
Fragments arranged by size:
5336 311 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3877 4058 4221 3877
Size: 181 163 5583
Fragments arranged by size:
5583 181 163
BtsI GCAGTG_nn'
Cuts at: 1569 3170 1569
Size: 1601 4326
DraI TTT'AAA
Cuts at: 2926 2926
Size: 5927
DrdI GACnn_nn'nnGTC
Cuts at: 1203 3633 4491 1203
Size: 2430 858 2639
Fragments arranged by size:
2639 2430 858
EaeI y'GGCC_r
Cuts at: 659 1056 1946 1955 2691 2901 3512 3686 4077
Size: 397 890 9 736 210 611 174 391
Cuts at: 4077 4104 5449 659
Size: 27 1345 1137
Fragments arranged by size:
1345 1137 890 736 611 397 391 210
174 27 9
EagI C'GGCC_G
Cuts at: 2901 3512 2901
Size: 611 5316
EarI CTCTTCn'nnn_
Cuts at: 1281 1526 3950 4160 1281
Size: 245 2424 210 3048
Fragments arranged by size:
3048 2424 245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 753 2628 2681 4135 4445 4591 5737 753
Size: 1875 53 1454 310 146 1146 943
Fragments arranged by size:
1875 1454 1146 943 310 146 53
EcoICRI GAG'CTC
Cuts at: 2939 2939
Size: 5927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 988 1498 1627 1734 2446 2727 2872 3752
Size: 693 510 129 107 712 281 145 880
Cuts at: 3752 4184 4931 5199 295
Size: 432 747 268 1023
Fragments arranged by size:
1023 880 747 712 693 510 432 281
268 145 129 107
EcoNI CCTnn'n_nnAGG
Cuts at: 1227 1701 1227
Size: 474 5453
EcoO109I rG'GnC_Cy
Cuts at: 210 270 743 1190 1385 1386 1510 1694 1725
Size: 60 473 447 195 1 124 184 31
Cuts at: 1725 1896 2106 2274 2555 2700 2737 2758 2870
Size: 171 210 168 281 145 37 21 112
Cuts at: 2870 3066 210
Size: 196 3071
Fragments arranged by size:
3071 473 447 281 210 196 195 184
171 168 145 124 112 60 37 31
21 1
EcoRI G'AATT_C
Cuts at: 2931 2931
Size: 5927
EcoRV GAT'ATC
Cuts at: 1767 1767
Size: 5927
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3946 3946
Size: 5927
FseI GG_CCGG'CC
Cuts at: 2790 2790
Size: 5927
FspI TGC'GCA
Cuts at: 3708 3708
Size: 5927
HaeII r_GCGC'y
Cuts at: 1979 2509 3361 3609 4631 5610 1979
Size: 530 852 248 1022 979 2296
Fragments arranged by size:
2296 1022 979 852 530 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 815 847 3769 3799 3801 3831 815
Size: 32 2922 30 2 30 2911
Fragments arranged by size:
2922 2911 32 30 30 2
HincII GTy'rAC
Cuts at: 2966 2966
Size: 5927
HindIII A'AGCT_T
Cuts at: 970 970
Size: 5927
KasI G'GCGC_C
Cuts at: 1975 3357 3605 1975
Size: 1382 248 4297
Fragments arranged by size:
4297 1382 248
KpnI G_GTAC'C
Cuts at: 2947 2947
Size: 5927
MluI A'CGCG_T
Cuts at: 5360 5360
Size: 5927
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 677 4598 4782 677
Size: 3921 184 1822
Fragments arranged by size:
3921 1822 184
MscI TGG'CCA
Cuts at: 661 1948 3688 661
Size: 1287 1740 2900
Fragments arranged by size:
2900 1740 1287
MslI CAynn'nnrTG
Cuts at: 1444 3916 4043 1444
Size: 2472 127 3328
Fragments arranged by size:
3328 2472 127
MspA1I CmG'CkG
Cuts at: 697 1326 1339 2577 3038 3352 3712 4725 4970
Size: 629 13 1238 461 314 360 1013 245
Cuts at: 4970 697
Size: 1654
Fragments arranged by size:
1654 1238 1013 629 461 360 314 245
13
NaeI GCC'GGC
Cuts at: 2788 4108 2788
Size: 1320 4607
NarI GG'CG_CC
Cuts at: 1976 3358 3606 1976
Size: 1382 248 4297
Fragments arranged by size:
4297 1382 248
NcoI C'CATG_G
Cuts at: 1817 1817
Size: 5927
NgoMIV G'CCGG_C
Cuts at: 2786 4106 2786
Size: 1320 4607
NspI r_CATG'y
Cuts at: 2980 3480 4387 2980
Size: 500 907 4520
Fragments arranged by size:
4520 907 500
PasI CC'CwG_GG
Cuts at: 515 994 1867 1971 2279 515
Size: 479 873 104 308 4163
Fragments arranged by size:
4163 873 479 308 104
PciI A'CATG_T
Cuts at: 4383 4383
Size: 5927
PflMI CCAn_nnn'nTGG
Cuts at: 447 813 1735 447
Size: 366 922 4639
Fragments arranged by size:
4639 922 366
PfoI T'CCnGG_A
Cuts at: 1526 1526
Size: 5927
PmeI GTTT'AAAC
Cuts at: 2926 2926
Size: 5927
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1147 1179 5066 5098 1147
Size: 32 3887 32 1976
Fragments arranged by size:
3887 1976 32 32
PpuMI rG'GwC_Cy
Cuts at: 210 1510 1725 1896 2274 2737 2758 210
Size: 1300 215 171 378 463 21 3379
Fragments arranged by size:
3379 1300 463 378 215 171 21
PspOMI G'GGCC_C
Cuts at: 742 1385 2106 2353 2699 2869 742
Size: 643 721 247 346 170 3800
Fragments arranged by size:
3800 721 643 346 247 170
PspXI vC'TCGA_Gb
Cuts at: 2720 2720
Size: 5927
PstI C_TGCA'G
Cuts at: 297 596 2974 3659 297
Size: 299 2378 685 2565
Fragments arranged by size:
2565 2378 685 299
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5927
PvuII CAG'CTG
Cuts at: 697 3352 3712 697
Size: 2655 360 2912
Fragments arranged by size:
2912 2655 360
RsrII CG'GwC_CG
Cuts at: 4122 4122
Size: 5927
SacI G_AGCT'C
Cuts at: 2941 2941
Size: 5927
SalI G'TCGA_C
Cuts at: 2964 2964
Size: 5927
SanDI GG'GwC_CC
Cuts at: 2274 2758 2274
Size: 484 5443
SapI GCTCTTCn'nnn_
Cuts at: 3950 4160 3950
Size: 210 5717
SbfI CC_TGCA'GG
Cuts at: 2974 2974
Size: 5927
ScaI AGT'ACT
Cuts at: 5444 5444
Size: 5927
SfcI C'TryA_G
Cuts at: 33 293 592 722 1545 2970 3655 4648 4839
Size: 260 299 130 823 1425 685 993 191
Cuts at: 4839 33
Size: 1121
Fragments arranged by size:
1425 1121 993 823 685 299 260 191
130
SfiI GGCCn_nnn'nGGCC
Cuts at: 1954 1954
Size: 5927
SfoI GGC'GCC
Cuts at: 1977 3359 3607 1977
Size: 1382 248 4297
Fragments arranged by size:
4297 1382 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5927
SmaI CCC'GGG
Cuts at: 358 2400 2763 2949 358
Size: 2042 363 186 3336
Fragments arranged by size:
3336 2042 363 186
SmlI C'TyrA_G
Cuts at: 2720 3082 3997 4489 4751 2720
Size: 362 915 492 262 3896
Fragments arranged by size:
3896 915 492 362 262
SnaBI TAC'GTA
Cuts at: 2919 2919
Size: 5927
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5927
SphI G_CATG'C
Cuts at: 2980 3480 2980
Size: 500 5427
StuI AGG'CCT
Cuts at: 811 1030 811
Size: 219 5708
StyI C'CwwG_G
Cuts at: 738 812 941 1452 1817 2129 2309 3061 3415
Size: 74 129 511 365 312 180 752 354
Cuts at: 3415 738
Size: 3250
Fragments arranged by size:
3250 752 511 365 354 312 180 129
74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 661 1102 1242 1369 3510 3643 4102 4311 661
Size: 441 140 127 2141 133 459 209 2277
Fragments arranged by size:
2277 2141 459 441 209 140 133 127
TatI w'GTAC_w
Cuts at: 683 5442 683
Size: 4759 1168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 355 387 788 820 943 975 5444 5476 355
Size: 32 401 32 123 32 4469 32 806
Fragments arranged by size:
4469 806 401 123 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 829 3724 829
Size: 2895 3032
XbaI T'CTAG_A
Cuts at: 2958 2958
Size: 5927
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1758 3422 1736
Size: 22 1664 4241
Fragments arranged by size:
4241 1664 22
XhoI C'TCGA_G
Cuts at: 2720 2720
Size: 5927
XmaI C'CCGG_G
Cuts at: 356 2398 2761 2947 356
Size: 2042 363 186 3336
Fragments arranged by size:
3336 2042 363 186
XmnI GAAnn'nnTTC
Cuts at: 553 553
Size: 5927
ZraI GAC'GTC
Cuts at: 2029 2829 2029
Size: 800 5127
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PsrI SacII SexAI SgrAI SrfI SspI SwaI