Restriction map for
pF1KSDA1872
(Circular) (Six-base) MAPSORT of: pF1KSDA1872.seq Check: 3819 from: 1 to: 4252
pF1KSDA1872 4252 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1288 1288
Size: 4252
Acc65I G'GTAC_C
Cuts at: 1266 1266
Size: 4252
AclI AA'CG_TT
Cuts at: 4092 4092
Size: 4252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 940 1129 1184 2075 2507 3256 3524 895
Size: 45 189 55 891 432 749 268 1623
Fragments arranged by size:
1623 891 749 432 268 189 55 45
AflIII A'CryG_T
Cuts at: 1062 1602 2708 3685 1062
Size: 540 1106 977 1629
Fragments arranged by size:
1629 1106 977 540
AgeI A'CCGG_T
Cuts at: 2640 2640
Size: 4252
AleI CACnn'nnGTG
Cuts at: 493 493
Size: 4252
AlwNI CAG_nnn'CTG
Cuts at: 1555 3124 1555
Size: 1569 2683
ApaLI G'TGCA_C
Cuts at: 1021 3022 1021
Size: 2001 2251
ApoI r'AATT_y
Cuts at: 279 1254 3605 3861 279
Size: 975 2351 256 670
Fragments arranged by size:
2351 975 670 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4252
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4252
AvaI C'yCGr_G
Cuts at: 517 1270 517
Size: 753 3499
BamHI G'GATC_C
Cuts at: 1275 1275
Size: 4252
BanI G'GyrC_C
Cuts at: 578 835 1182 1266 1680 1928 1963 578
Size: 257 347 84 414 248 35 2867
Fragments arranged by size:
2867 414 347 257 248 84 35
BanII G_rGCy'C
Cuts at: 797 1264 2294 797
Size: 467 1030 2755
Fragments arranged by size:
2755 1030 467
BbeI G_GCGC'C
Cuts at: 582 1684 1932 582
Size: 1102 248 2902
Fragments arranged by size:
2902 1102 248
BbsI GAAGACnn'nnnn_
Cuts at: 257 313 488 696 1040 257
Size: 56 175 208 344 3469
Fragments arranged by size:
3469 344 208 175 56
BbvCI CC'TCA_GC
Cuts at: 806 806
Size: 4252
BciVI GTATCCnnnnn_n'
Cuts at: 375 2148 2911 4252 375
Size: 1773 763 1341 375
Fragments arranged by size:
1773 1341 763 375
BclI T'GATC_A
Cuts at: 596 929 1770 596
Size: 333 841 3078
Fragments arranged by size:
3078 841 333
BglII A'GATC_T
Cuts at: 192 1765 3804 192
Size: 1573 2039 640
Fragments arranged by size:
2039 1573 640
BlpI GC'TnA_GC
Cuts at: 1362 1362
Size: 4252
Bme1580I G_kGCm'C
Cuts at: 1025 1875 1968 3026 1025
Size: 850 93 1058 2251
Fragments arranged by size:
2251 1058 850 93
BmgBI CAC'GTC
Cuts at: 168 1065 168
Size: 897 3355
BmrI ACTGGGnnnn_n'
Cuts at: 1462 1543 1878 4024 1462
Size: 81 335 2146 1690
Fragments arranged by size:
2146 1690 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 456 2617 456
Size: 2161 2091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 493 1060 466
Size: 27 567 3658
Fragments arranged by size:
3658 567 27
Bpu10I CC'TnA_GC
Cuts at: 806 806
Size: 4252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1426 2305 2799 3097 1426
Size: 879 494 298 2581
Fragments arranged by size:
2581 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 415 427 1215 3956 415
Size: 12 788 2741 711
Fragments arranged by size:
2741 788 711 12
BsaAI yAC'GTr
Cuts at: 1020 1242 1603 1020
Size: 222 361 3669
Fragments arranged by size:
3669 361 222
BsaBI GATnn'nnATC
Cuts at: 6 1764 3803 6
Size: 1758 2039 455
Fragments arranged by size:
2039 1758 455
BsaHI Gr'CG_yC
Cuts at: 579 1681 1929 2633 4129 579
Size: 1102 248 704 1496 702
Fragments arranged by size:
1496 1102 704 702 248
BsaWI w'CCGG_w
Cuts at: 149 899 1439 1960 2640 2914 3061 3472 149
Size: 750 540 521 680 274 147 411 929
Fragments arranged by size:
929 750 680 540 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 246 276 246
Size: 30 4222
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 254 254
Size: 4252
BseYI C'CCAG_C
Cuts at: 1136 1675 2433 3012 3508 1136
Size: 539 758 579 496 1880
Fragments arranged by size:
1880 758 579 539 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 4252
BsiEI CG_ry'CG
Cuts at: 104 900 1792 1838 3048 104
Size: 796 892 46 1210 1308
Fragments arranged by size:
1308 1210 892 796 46
BsiHKAI G_wGCw'C
Cuts at: 617 797 1025 1264 2042 2232 3026 617
Size: 180 228 239 778 190 794 1843
Fragments arranged by size:
1843 794 778 239 228 190 180
BsmBI CGTCTCn'nnnn_
Cuts at: 211 211
Size: 4252
Bsp1286I G_dGCh'C
Cuts at: 617 797 1025 1264 1875 1968 2042 2232 2294
Size: 180 228 239 611 93 74 190 62
Cuts at: 2294 3026 617
Size: 732 1843
Fragments arranged by size:
1843 732 611 239 228 190 180 93
74 62
BspEI T'CCGG_A
Cuts at: 149 3472 149
Size: 3323 929
BspHI T'CATG_A
Cuts at: 3428 3428
Size: 4252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 635 1300 1514 1816 2197 635
Size: 665 214 302 381 2690
Fragments arranged by size:
2690 665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2542 2596 4082 4247 50
Size: 2492 54 1486 165 55
Fragments arranged by size:
2492 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2162 2162
Size: 4252
BsrFI r'CCGG_y
Cuts at: 2248 2429 2640 2248
Size: 181 211 3860
Fragments arranged by size:
3860 211 181
BssSI C'ACGA_G
Cuts at: 945 2521 2881 945
Size: 1576 360 2316
Fragments arranged by size:
2316 1576 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1627 1627
Size: 4252
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4252
BstEII G'GTnAC_C
Cuts at: 657 657
Size: 4252
BstYI r'GATC_y
Cuts at: 1 192 531 1010 1070 1275 1742 1765 2100
Size: 191 339 479 60 205 467 23 335
Cuts at: 2100 2346 3360 3446 3458 3804 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 479 467 449 346 339 335 246
205 191 86 60 23 12
BtgI C'CryG_G
Cuts at: 125 494 663 125
Size: 369 169 3714
Fragments arranged by size:
3714 369 169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2200 2381 2544 2200
Size: 181 163 3908
Fragments arranged by size:
3908 181 163
BtsI GCAGTG_nn'
Cuts at: 1493 1493
Size: 4252
DraI TTT'AAA
Cuts at: 1249 1249
Size: 4252
DrdI GACnn_nn'nnGTC
Cuts at: 1956 2816 1956
Size: 860 3392
EaeI y'GGCC_r
Cuts at: 497 968 1835 2009 2400 2427 3774 497
Size: 471 867 174 391 27 1347 975
Fragments arranged by size:
1347 975 867 471 391 174 27
EagI C'GGCC_G
Cuts at: 1835 1835
Size: 4252
EarI CTCTTCn'nnn_
Cuts at: 209 772 1091 1160 2273 2483 209
Size: 563 319 69 1113 210 1978
Fragments arranged by size:
1978 1113 563 319 210 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 603 864 2458 2770 2916 4062 603
Size: 261 1594 312 146 1146 793
Fragments arranged by size:
1594 1146 793 312 261 146
EcoICRI GAG'CTC
Cuts at: 795 1262 795
Size: 467 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 493 895 940 1060 1129 1184 2075 2507
Size: 27 402 45 120 69 55 891 432
Cuts at: 2507 3256 3524 466
Size: 749 268 1194
Fragments arranged by size:
1194 891 749 432 402 268 120 69
55 45 27
EcoO109I rG'GnC_Cy
Cuts at: 356 1133 1389 356
Size: 777 256 3219
Fragments arranged by size:
3219 777 256
EcoRI G'AATT_C
Cuts at: 1254 1254
Size: 4252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 4252
FspI TGC'GCA
Cuts at: 2031 2031
Size: 4252
HaeII r_GCGC'y
Cuts at: 569 582 1684 1932 2956 3935 569
Size: 13 1102 248 1024 979 886
Fragments arranged by size:
1102 1024 979 886 248 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 2092 2122 2124 2154 244
Size: 32 1816 30 2 30 2342
Fragments arranged by size:
2342 1816 32 30 30 2
HincII GTy'rAC
Cuts at: 1289 1289
Size: 4252
KasI G'GCGC_C
Cuts at: 578 1680 1928 578
Size: 1102 248 2902
Fragments arranged by size:
2902 1102 248
KpnI G_GTAC'C
Cuts at: 1270 1270
Size: 4252
MluI A'CGCG_T
Cuts at: 3685 3685
Size: 4252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2923 3107 2923
Size: 184 4068
MscI TGG'CCA
Cuts at: 499 970 2011 499
Size: 471 1041 2740
Fragments arranged by size:
2740 1041 471
MslI CAynn'nnrTG
Cuts at: 493 652 820 1054 2239 2366 493
Size: 159 168 234 1185 127 2379
Fragments arranged by size:
2379 1185 234 168 159 127
MspA1I CmG'CkG
Cuts at: 1361 1675 2035 3050 3295 1361
Size: 314 360 1015 245 2318
Fragments arranged by size:
2318 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2431 2431
Size: 4252
NarI GG'CG_CC
Cuts at: 579 1681 1929 579
Size: 1102 248 2902
Fragments arranged by size:
2902 1102 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4252
NgoMIV G'CCGG_C
Cuts at: 2429 2429
Size: 4252
NruI TCG'CGA
Cuts at: 2613 2613
Size: 4252
NspI r_CATG'y
Cuts at: 516 1236 1303 1803 2712 516
Size: 720 67 500 909 2056
Fragments arranged by size:
2056 909 720 500 67
PciI A'CATG_T
Cuts at: 2708 2708
Size: 4252
PmeI GTTT'AAAC
Cuts at: 1249 1249
Size: 4252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3391 3423 3391
Size: 32 4220
PpuMI rG'GwC_Cy
Cuts at: 356 356
Size: 4252
PstI C_TGCA'G
Cuts at: 1297 1982 1297
Size: 685 3567
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4252
PvuII CAG'CTG
Cuts at: 1675 2035 1675
Size: 360 3892
RsrII CG'GwC_CG
Cuts at: 2445 2445
Size: 4252
SacI G_AGCT'C
Cuts at: 797 1264 797
Size: 467 3785
SalI G'TCGA_C
Cuts at: 1287 1287
Size: 4252
SanDI GG'GwC_CC
Cuts at: 356 356
Size: 4252
SapI GCTCTTCn'nnn_
Cuts at: 2273 2483 2273
Size: 210 4042
SbfI CC_TGCA'GG
Cuts at: 1297 1297
Size: 4252
ScaI AGT'ACT
Cuts at: 3769 3769
Size: 4252
SfcI C'TryA_G
Cuts at: 33 1293 1978 2973 3164 33
Size: 1260 685 995 191 1121
Fragments arranged by size:
1260 1121 995 685 191
SfoI GGC'GCC
Cuts at: 580 1682 1930 580
Size: 1102 248 2902
Fragments arranged by size:
2902 1102 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4252
SmaI CCC'GGG
Cuts at: 519 1272 519
Size: 753 3499
SmlI C'TyrA_G
Cuts at: 1405 2320 2814 3076 1405
Size: 915 494 262 2581
Fragments arranged by size:
2581 915 494 262
SnaBI TAC'GTA
Cuts at: 1242 1242
Size: 4252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4252
SphI G_CATG'C
Cuts at: 1303 1803 1303
Size: 500 3752
StuI AGG'CCT
Cuts at: 686 686
Size: 4252
StyI C'CwwG_G
Cuts at: 125 1384 1738 125
Size: 1259 354 2639
Fragments arranged by size:
2639 1259 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 451 676 1833 1966 2425 2636 451
Size: 225 1157 133 459 211 2067
Fragments arranged by size:
2067 1157 459 225 211 133
TatI w'GTAC_w
Cuts at: 3767 3767
Size: 4252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 246 278 3769 3801 246
Size: 32 3491 32 697
Fragments arranged by size:
3491 697 32 32
Tth111I GACn'n_nGTC
Cuts at: 1081 2047 1081
Size: 966 3286
XbaI T'CTAG_A
Cuts at: 1281 1281
Size: 4252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 1745 150
Size: 1595 2657
XmaI C'CCGG_G
Cuts at: 517 1270 517
Size: 753 3499
XmnI GAAnn'nnTTC
Cuts at: 194 766 194
Size: 572 3680
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI