Restriction map for
pF1KSDA1874
(Circular) (Six-base) MAPSORT of: pF1KSDA1874.seq Check: 3053 from: 1 to: 4700
pF1KSDA1874 4700 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 704 1738 704
Size: 1034 3666
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 4540 4540
Size: 4700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 764 1449 1697 2525 2957 3704 3972 479
Size: 285 685 248 828 432 747 268 1207
Fragments arranged by size:
1207 828 747 685 432 285 268 248
AflIII A'CryG_T
Cuts at: 2052 3156 4133 2052
Size: 1104 977 2619
Fragments arranged by size:
2619 1104 977
AgeI A'CCGG_T
Cuts at: 3088 3088
Size: 4700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 540 540
Size: 4700
AlwNI CAG_nnn'CTG
Cuts at: 2005 3572 2005
Size: 1567 3133
ApaLI G'TGCA_C
Cuts at: 3470 3470
Size: 4700
ApoI r'AATT_y
Cuts at: 666 1010 1079 1415 1499 1704 4053 4309 666
Size: 344 69 336 84 205 2349 256 1057
Fragments arranged by size:
2349 1057 344 336 256 205 84 69
AseI AT'TA_AT
Cuts at: 20 20
Size: 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 1720 1720
Size: 4700
BamHI G'GATC_C
Cuts at: 312 1725 312
Size: 1413 3287
BanI G'GyrC_C
Cuts at: 1716 2130 2378 2413 1716
Size: 414 248 35 4003
Fragments arranged by size:
4003 414 248 35
BanII G_rGCy'C
Cuts at: 165 1477 1714 2744 165
Size: 1312 237 1030 2121
Fragments arranged by size:
2121 1312 1030 237
BbeI G_GCGC'C
Cuts at: 2134 2382 2134
Size: 248 4452
BbsI GAAGACnn'nnnn_
Cuts at: 161 752 161
Size: 591 4109
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3359 4700 2598
Size: 761 1341 2598
Fragments arranged by size:
2598 1341 761
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BglII A'GATC_T
Cuts at: 2215 4252 2215
Size: 2037 2663
BlpI GC'TnA_GC
Cuts at: 1812 1812
Size: 4700
Bme1580I G_kGCm'C
Cuts at: 2325 2418 3474 2325
Size: 93 1056 3551
Fragments arranged by size:
3551 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1912 1993 2328 4472 1912
Size: 81 335 2144 2140
Fragments arranged by size:
2144 2140 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 944 1193 1277 1361 1445 358
Size: 586 249 84 84 84 3613
Fragments arranged by size:
3613 586 249 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1876 2755 3247 3545 448
Size: 1428 879 492 298 1603
Fragments arranged by size:
1603 1428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1089 4404 1089
Size: 3315 1385
BsaAI yAC'GTr
Cuts at: 1692 2053 1692
Size: 361 4339
BsaBI GATnn'nnATC
Cuts at: 6 2214 4251 6
Size: 2208 2037 455
Fragments arranged by size:
2208 2037 455
BsaHI Gr'CG_yC
Cuts at: 2131 2379 3081 4577 2131
Size: 248 702 1496 2254
Fragments arranged by size:
2254 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1591 1889 2410 3088 3362 3509 3920 1591
Size: 298 521 678 274 147 411 2371
Fragments arranged by size:
2371 678 521 411 298 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 310
Size: 4700
BseYI C'CCAG_C
Cuts at: 2125 2883 3460 3956 2125
Size: 758 577 496 2869
Fragments arranged by size:
2869 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 1171 1659 302
Size: 869 488 3343
Fragments arranged by size:
3343 869 488
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3496 104
Size: 2138 46 1208 1308
Fragments arranged by size:
2138 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 165 205 1393 1477 1714 2492 2682 3474 165
Size: 40 1188 84 237 778 190 792 1391
Fragments arranged by size:
1391 1188 792 778 237 190 84 40
BsmI GAATG_Cn'
Cuts at: 1027 1036 1111 1363 1027
Size: 9 75 252 4364
Fragments arranged by size:
4364 252 75 9
Bsp1286I G_dGCh'C
Cuts at: 165 205 1393 1477 1714 2325 2418 2492 2682
Size: 40 1188 84 237 611 93 74 190
Cuts at: 2682 2744 3474 165
Size: 62 730 1391
Fragments arranged by size:
1391 1188 730 611 237 190 93 84
74 62 40
BspEI T'CCGG_A
Cuts at: 3920 3920
Size: 4700
BspHI T'CATG_A
Cuts at: 3876 3876
Size: 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 949 1750 1964 2266 2647 949
Size: 801 214 302 381 3002
Fragments arranged by size:
3002 801 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2992 3046 4530 4695 50
Size: 2942 54 1484 165 55
Fragments arranged by size:
2942 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 951 1116 1302 2612 951
Size: 165 186 1310 3039
Fragments arranged by size:
3039 1310 186 165
BsrFI r'CCGG_y
Cuts at: 2698 2879 3088 2698
Size: 181 209 4310
Fragments arranged by size:
4310 209 181
BssSI C'ACGA_G
Cuts at: 2971 3329 2971
Size: 358 4342
BstAPI GCAn_nnn'nTGC
Cuts at: 1031 2077 1031
Size: 1046 3654
BstBI TT'CG_AA
Cuts at: 110 3061 110
Size: 2951 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 299 299
Size: 4700
BstYI r'GATC_y
Cuts at: 1 244 312 763 1725 2192 2215 2550 2796
Size: 243 68 451 962 467 23 335 246
Cuts at: 2796 3808 3894 3906 4252 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 962 467 451 449 346 335 246
243 86 68 23 12
BstZ17I GTA'TAC
Cuts at: 705 705
Size: 4700
BtgI C'CryG_G
Cuts at: 125 278 125
Size: 153 4547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2650 2831 2994 2650
Size: 181 163 4356
Fragments arranged by size:
4356 181 163
BtsI GCAGTG_nn'
Cuts at: 1032 1368 1943 1032
Size: 336 575 3789
Fragments arranged by size:
3789 575 336
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 933 968 1098 1133 933
Size: 35 130 35 4500
Fragments arranged by size:
4500 130 35 35
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 2406 3264 2406
Size: 858 3842
EaeI y'GGCC_r
Cuts at: 239 275 2285 2459 2850 2877 4222 239
Size: 36 2010 174 391 27 1345 717
Fragments arranged by size:
2010 1345 717 391 174 36 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 193 203 883 1673 2723 2933 193
Size: 10 680 790 1050 210 1960
Fragments arranged by size:
1960 1050 790 680 210 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3218 3364 4510 2908
Size: 310 146 1146 3098
Fragments arranged by size:
3098 1146 310 146
EcoICRI GAG'CTC
Cuts at: 163 1475 1712 163
Size: 1312 237 3151
Fragments arranged by size:
3151 1312 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 479 764 944 1193 1277 1361 1445 1449
Size: 121 285 180 249 84 84 84 4
Cuts at: 1449 1697 2525 2957 3704 3972 358
Size: 248 828 432 747 268 1086
Fragments arranged by size:
1086 828 747 432 285 268 249 248
180 121 84 84 84 4
EcoNI CCTnn'n_nnAGG
Cuts at: 155 155
Size: 4700
EcoO109I rG'GnC_Cy
Cuts at: 1839 1839
Size: 4700
EcoRI G'AATT_C
Cuts at: 666 1010 1079 1415 1499 1704 666
Size: 344 69 336 84 205 3662
Fragments arranged by size:
3662 344 336 205 84 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 607 1059 2719 607
Size: 452 1660 2588
Fragments arranged by size:
2588 1660 452
FspI TGC'GCA
Cuts at: 2481 2481
Size: 4700
HaeII r_GCGC'y
Cuts at: 2134 2382 3404 4383 2134
Size: 248 1022 979 2451
Fragments arranged by size:
2451 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1380 1412 2542 2572 2574 2604 1380
Size: 32 1130 30 2 30 3476
Fragments arranged by size:
3476 1130 32 30 30 2
HincII GTy'rAC
Cuts at: 1739 1739
Size: 4700
HindIII A'AGCT_T
Cuts at: 424 714 792 1208 1629 424
Size: 290 78 416 421 3495
Fragments arranged by size:
3495 421 416 290 78
KasI G'GCGC_C
Cuts at: 2130 2378 2130
Size: 248 4452
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1068 3371 3555 1068
Size: 2303 184 2213
Fragments arranged by size:
2303 2213 184
MscI TGG'CCA
Cuts at: 277 2461 277
Size: 2184 2516
MslI CAynn'nnrTG
Cuts at: 661 695 1356 2689 2816 661
Size: 34 661 1333 127 2545
Fragments arranged by size:
2545 1333 661 127 34
MspA1I CmG'CkG
Cuts at: 1811 2125 2485 3498 3743 1811
Size: 314 360 1013 245 2768
Fragments arranged by size:
2768 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2881 2881
Size: 4700
NarI GG'CG_CC
Cuts at: 2131 2379 2131
Size: 248 4452
NcoI C'CATG_G
Cuts at: 125 278 125
Size: 153 4547
NdeI CA'TA_TG
Cuts at: 1353 1353
Size: 4700
NgoMIV G'CCGG_C
Cuts at: 2879 2879
Size: 4700
NspI r_CATG'y
Cuts at: 1753 2253 3160 1753
Size: 500 907 3293
Fragments arranged by size:
3293 907 500
PciI A'CATG_T
Cuts at: 3156 3156
Size: 4700
PflMI CCAn_nnn'nTGG
Cuts at: 399 399
Size: 4700
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 343 375 3839 3871 343
Size: 32 3464 32 1172
Fragments arranged by size:
3464 1172 32 32
PsiI TTA'TAA
Cuts at: 836 1271 836
Size: 435 4265
PstI C_TGCA'G
Cuts at: 1747 2432 1747
Size: 685 4015
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 2125 2485 2125
Size: 360 4340
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 165 1477 1714 165
Size: 1312 237 3151
Fragments arranged by size:
3151 1312 237
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 193 1673 2723 2933 193
Size: 1480 1050 210 1960
Fragments arranged by size:
1960 1480 1050 210
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 4217 4217
Size: 4700
SfcI C'TryA_G
Cuts at: 33 345 1743 2428 3421 3612 33
Size: 312 1398 685 993 191 1121
Fragments arranged by size:
1398 1121 993 685 312 191
SfoI GGC'GCC
Cuts at: 2132 2380 2132
Size: 248 4452
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 1722 1722
Size: 4700
SmlI C'TyrA_G
Cuts at: 427 1855 2770 3262 3524 427
Size: 1428 915 492 262 1603
Fragments arranged by size:
1603 1428 915 492 262
SnaBI TAC'GTA
Cuts at: 1692 1692
Size: 4700
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1753 2253 1753
Size: 500 4200
StyI C'CwwG_G
Cuts at: 125 278 1834 2188 125
Size: 153 1556 354 2637
Fragments arranged by size:
2637 1556 354 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2283 2416 2875 3084 2283
Size: 133 459 209 3899
Fragments arranged by size:
3899 459 209 133
TatI w'GTAC_w
Cuts at: 1138 4215 1138
Size: 3077 1623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 4668
Tth111I GACn'n_nGTC
Cuts at: 2497 2497
Size: 4700
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2195 2195
Size: 4700
XmaI C'CCGG_G
Cuts at: 1720 1720
Size: 4700
XmnI GAAnn'nnTTC
Cuts at: 715 1042 1462 715
Size: 327 420 3953
Fragments arranged by size:
3953 420 327
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII
Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI