Restriction map for
pF1KSDA1882
(Circular) (Six-base) MAPSORT of: pF1KSDA1882.seq Check: 3329 from: 1 to: 6581
pF1KSDA1882 6581 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2849 3164 2849
Size: 315 6266
AccI GT'mk_AC
Cuts at: 1529 3619 1529
Size: 2090 4491
Acc65I G'GTAC_C
Cuts at: 3597 3597
Size: 6581
AclI AA'CG_TT
Cuts at: 6421 6421
Size: 6581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 980 1106 1213 2753 3118 4406 4838 5585
Size: 776 126 107 1540 365 1288 432 747
Cuts at: 5585 5853 204
Size: 268 932
Fragments arranged by size:
1540 1288 932 776 747 432 365 268
126 107
AfeI AGC'GCT
Cuts at: 1285 1285
Size: 6581
AflIII A'CryG_T
Cuts at: 806 1182 1817 3545 3933 5037 6014 806
Size: 376 635 1728 388 1104 977 1373
Fragments arranged by size:
1728 1373 1104 977 635 388 376
AgeI A'CCGG_T
Cuts at: 1716 4969 1716
Size: 3253 3328
AhdI GACnn_n'nnGTC
Cuts at: 1608 1608
Size: 6581
AleI CACnn'nnGTG
Cuts at: 517 1865 3548 517
Size: 1348 1683 3550
Fragments arranged by size:
3550 1683 1348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2248 3526 3566 2248
Size: 1278 40 5263
Fragments arranged by size:
5263 1278 40
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1642 1674 1642
Size: 32 6549
AlwNI CAG_nnn'CTG
Cuts at: 171 883 1490 2141 3886 5453 171
Size: 712 607 651 1745 1567 1299
Fragments arranged by size:
1745 1567 1299 712 651 607
ApaI G_GGCC'C
Cuts at: 3296 3441 3296
Size: 145 6436
ApaLI G'TGCA_C
Cuts at: 3340 3541 5351 3340
Size: 201 1810 4570
Fragments arranged by size:
4570 1810 201
ApoI r'AATT_y
Cuts at: 1222 1277 2700 3585 5934 6190 1222
Size: 55 1423 885 2349 256 1613
Fragments arranged by size:
2349 1613 1423 885 256 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 6581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6581
AvaI C'yCGr_G
Cuts at: 313 2615 2644 2721 2798 3044 3433 3601 313
Size: 2302 29 77 77 246 389 168 3293
Fragments arranged by size:
3293 2302 389 246 168 77 77 29
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2273 2306 2273
Size: 33 6548
BamHI G'GATC_C
Cuts at: 1455 3606 1455
Size: 2151 4430
BanI G'GyrC_C
Cuts at: 299 725 1012 1719 3165 3597 4011 4259 4294
Size: 426 287 707 1446 432 414 248 35
Cuts at: 4294 299
Size: 2586
Fragments arranged by size:
2586 1446 707 432 426 414 287 248
35
BanII G_rGCy'C
Cuts at: 930 1307 1685 3280 3296 3382 3441 3595 4625
Size: 377 378 1595 16 86 59 154 1030
Cuts at: 4625 930
Size: 2886
Fragments arranged by size:
2886 1595 1030 378 377 154 86 59
16
BbeI G_GCGC'C
Cuts at: 3169 4015 4263 3169
Size: 846 248 5487
Fragments arranged by size:
5487 846 248
BbsI GAAGACnn'nnnn_
Cuts at: 468 1094 2115 2686 2847 468
Size: 626 1021 571 161 4202
Fragments arranged by size:
4202 1021 626 571 161
BbvCI CC'TCA_GC
Cuts at: 155 155
Size: 6581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2590 2624 2590
Size: 34 6547
BciVI GTATCCnnnnn_n'
Cuts at: 4479 5240 6581 4479
Size: 761 1341 4479
Fragments arranged by size:
4479 1341 761
BclI T'GATC_A
Cuts at: 4101 4101
Size: 6581
BglI GCCn_nnn'nGGC
Cuts at: 3236 3559 3236
Size: 323 6258
BglII A'GATC_T
Cuts at: 437 2239 4096 6133 437
Size: 1802 1857 2037 885
Fragments arranged by size:
2037 1857 1802 885
BlpI GC'TnA_GC
Cuts at: 3693 3693
Size: 6581
Bme1580I G_kGCm'C
Cuts at: 990 1017 1724 3296 3344 3441 3545 3555 4206
Size: 27 707 1572 48 97 104 10 651
Cuts at: 4206 4299 5355 990
Size: 93 1056 2216
Fragments arranged by size:
2216 1572 1056 707 651 104 97 93
48 27 10
BmgBI CAC'GTC
Cuts at: 519 1063 519
Size: 544 6037
BmrI ACTGGGnnnn_n'
Cuts at: 2050 2448 3530 3793 3874 4209 6353 2050
Size: 398 1082 263 81 335 2144 2278
Fragments arranged by size:
2278 2144 1082 398 335 263 81
BmtI G_CTAG'C
Cuts at: 1778 1778
Size: 6581
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 944 2397 4948 944
Size: 1453 2551 2577
Fragments arranged by size:
2577 2551 1453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 2392 2735 3043 3073 3102 3169 3274 3366
Size: 1770 343 308 30 29 67 105 92
Cuts at: 3366 3577 622
Size: 211 3626
Fragments arranged by size:
3626 1770 343 308 211 105 92 67
30 29
Bpu10I CC'TnA_GC
Cuts at: 155 1078 155
Size: 923 5658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2445 3757 4636 5128 5426 2445
Size: 1312 879 492 298 3600
Fragments arranged by size:
3600 1312 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6285 6285
Size: 6581
BsaAI yAC'GTr
Cuts at: 426 750 1183 2676 3546 3573 3934 426
Size: 324 433 1493 870 27 361 3073
Fragments arranged by size:
3073 1493 870 433 361 324 27
BsaBI GATnn'nnATC
Cuts at: 6 4095 6132 6
Size: 4089 2037 455
Fragments arranged by size:
4089 2037 455
BsaHI Gr'CG_yC
Cuts at: 3166 4012 4260 4962 6458 3166
Size: 846 248 702 1496 3289
Fragments arranged by size:
3289 1496 846 702 248
BsaWI w'CCGG_w
Cuts at: 1716 3272 3479 3770 4291 4969 5243 5390 5801
Size: 1556 207 291 521 678 274 147 411
Cuts at: 5801 1716
Size: 2496
Fragments arranged by size:
2496 1556 678 521 411 291 274 207
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1381 1411 1993 2023 3352 3382 1381
Size: 30 582 30 1329 30 4580
Fragments arranged by size:
4580 1329 582 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 1408 3433 223
Size: 1185 2025 3371
Fragments arranged by size:
3371 2025 1185
BseYI C'CCAG_C
Cuts at: 272 1490 1806 2814 3069 4006 4764 5341 5837
Size: 1218 316 1008 255 937 758 577 496
Cuts at: 5837 272
Size: 1016
Fragments arranged by size:
1218 1016 1008 937 758 577 496 316
255
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 2228 2899 3340 3427 448
Size: 1780 671 441 87 3602
Fragments arranged by size:
3602 1780 671 441 87
BsiEI CG_ry'CG
Cuts at: 104 4123 4169 5377 104
Size: 4019 46 1208 1308
Fragments arranged by size:
4019 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 930 1307 3155 3344 3545 3595 4373 4563 5355
Size: 377 1848 189 201 50 778 190 792
Cuts at: 5355 930
Size: 2156
Fragments arranged by size:
2156 1848 792 778 377 201 190 189
50
BsmI GAATG_Cn'
Cuts at: 1146 1239 1146
Size: 93 6488
BsmBI CGTCTCn'nnnn_
Cuts at: 207 3372 207
Size: 3165 3416
Bsp1286I G_dGCh'C
Cuts at: 930 990 1017 1307 1685 1724 3155 3280 3296
Size: 60 27 290 378 39 1431 125 16
Cuts at: 3296 3344 3382 3441 3545 3555 3595 4206 4299
Size: 48 38 59 104 10 40 611 93
Cuts at: 4299 4373 4563 4625 5355 930
Size: 74 190 62 730 2156
Fragments arranged by size:
2156 1431 730 611 378 290 190 125
104 93 74 62 60 59 48 40
39 38 27 16 10
BspEI T'CCGG_A
Cuts at: 5801 5801
Size: 6581
BspHI T'CATG_A
Cuts at: 5757 5757
Size: 6581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2165 2592 2849 3164 3362 3631 3845 4147 4528
Size: 427 257 315 198 269 214 302 381
Cuts at: 4528 2165
Size: 4218
Fragments arranged by size:
4218 427 381 315 302 269 257 214
198
BsrBI CCG'CTC
Cuts at: 50 1878 3033 4873 4927 6411 6576 50
Size: 1828 1155 1840 54 1484 165 55
Fragments arranged by size:
1840 1828 1484 1155 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2251 4493 2251
Size: 2242 4339
BsrFI r'CCGG_y
Cuts at: 977 1716 2920 4579 4760 4969 977
Size: 739 1204 1659 181 209 2589
Fragments arranged by size:
2589 1659 1204 739 209 181
BssSI C'ACGA_G
Cuts at: 175 3487 4852 5210 175
Size: 3312 1365 358 1546
Fragments arranged by size:
3312 1546 1365 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3958 3958
Size: 6581
BstBI TT'CG_AA
Cuts at: 110 4942 110
Size: 4832 1749
BstEII G'GTnAC_C
Cuts at: 2107 2376 2107
Size: 269 6312
BstXI CCAn_nnnn'nTGG
Cuts at: 615 1998 3021 3521 615
Size: 1383 1023 500 3675
Fragments arranged by size:
3675 1383 1023 500
BstYI r'GATC_y
Cuts at: 1 224 437 647 1455 2239 2544 3056 3606
Size: 223 213 210 808 784 305 512 550
Cuts at: 3606 4073 4096 4431 4677 5689 5775 5787 6133
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6133 1
Size: 449
Fragments arranged by size:
1012 808 784 550 512 467 449 346
335 305 246 223 213 210 86 23
12
BtgI C'CryG_G
Cuts at: 1676 1866 2117 1676
Size: 190 251 6140
Fragments arranged by size:
6140 251 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1312 1368 1671 4531 4712 4875 1312
Size: 56 303 2860 181 163 3018
Fragments arranged by size:
3018 2860 303 181 163 56
BtsI GCAGTG_nn'
Cuts at: 154 1372 2839 3111 3181 3824 154
Size: 1218 1467 272 70 643 2911
Fragments arranged by size:
2911 1467 1218 643 272 70
DraI TTT'AAA
Cuts at: 3580 3580
Size: 6581
DrdI GACnn_nn'nnGTC
Cuts at: 4287 5145 4287
Size: 858 5723
EaeI y'GGCC_r
Cuts at: 389 400 979 1355 1783 2480 3417 4166 4340
Size: 11 579 376 428 697 937 749 174
Cuts at: 4340 4731 4758 6103 389
Size: 391 27 1345 867
Fragments arranged by size:
1345 937 867 749 697 579 428 391
376 174 27 11
EagI C'GGCC_G
Cuts at: 4166 4166
Size: 6581
EarI CTCTTCn'nnn_
Cuts at: 556 913 1265 1868 2036 3023 3451 4604 4814
Size: 357 352 603 168 987 428 1153 210
Cuts at: 4814 556
Size: 2323
Fragments arranged by size:
2323 1153 987 603 428 357 352 210
168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4789 5099 5245 6391 4789
Size: 310 146 1146 4979
Fragments arranged by size:
4979 1146 310 146
EcoICRI GAG'CTC
Cuts at: 928 1305 3593 928
Size: 377 2288 3916
Fragments arranged by size:
3916 2288 377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 622 980 1106 1213 2392 2735 2753 3043
Size: 418 358 126 107 1179 343 18 290
Cuts at: 3043 3073 3102 3118 3169 3274 3366 3577 4406
Size: 30 29 16 51 105 92 211 829
Cuts at: 4406 4838 5585 5853 204
Size: 432 747 268 932
Fragments arranged by size:
1179 932 829 747 432 418 358 343
290 268 211 126 107 105 92 51
30 29 18 16
EcoNI CCTnn'n_nnAGG
Cuts at: 1539 1539
Size: 6581
EcoO109I rG'GnC_Cy
Cuts at: 756 1403 2073 3292 3293 3437 3438 3720 756
Size: 647 670 1219 1 144 1 282 3617
Fragments arranged by size:
3617 1219 670 647 282 144 1 1
EcoRI G'AATT_C
Cuts at: 3585 3585
Size: 6581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2979 4600 2979
Size: 1621 4960
FspI TGC'GCA
Cuts at: 4362 4362
Size: 6581
HaeII r_GCGC'y
Cuts at: 1287 2026 3169 4015 4263 5285 6264 1287
Size: 739 1143 846 248 1022 979 1604
Fragments arranged by size:
1604 1143 1022 979 846 739 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 370 402 1594 1626 1993 2025 2204 2236 2263
Size: 32 1192 32 367 32 179 32 27
Cuts at: 2263 2295 3352 3384 4423 4453 4455 4485 370
Size: 32 1057 32 1039 30 2 30 2466
Fragments arranged by size:
2466 1192 1057 1039 367 179 32 32
32 32 32 32 30 30 27 2
HincII GTy'rAC
Cuts at: 1823 3620 1823
Size: 1797 4784
HindIII A'AGCT_T
Cuts at: 1732 1800 1732
Size: 68 6513
KasI G'GCGC_C
Cuts at: 3165 4011 4259 3165
Size: 846 248 5487
Fragments arranged by size:
5487 846 248
KpnI G_GTAC'C
Cuts at: 3601 3601
Size: 6581
MluI A'CGCG_T
Cuts at: 6014 6014
Size: 6581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1592 5252 5436 1592
Size: 3660 184 2737
Fragments arranged by size:
3660 2737 184
MscI TGG'CCA
Cuts at: 402 2482 4342 402
Size: 2080 1860 2641
Fragments arranged by size:
2641 2080 1860
MslI CAynn'nnrTG
Cuts at: 517 811 1865 2503 3548 4570 4697 517
Size: 294 1054 638 1045 1022 127 2401
Fragments arranged by size:
2401 1054 1045 1022 638 294 127
MspA1I CmG'CkG
Cuts at: 1932 1994 3021 3162 3692 4006 4366 5379 5624
Size: 62 1027 141 530 314 360 1013 245
Cuts at: 5624 1932
Size: 2889
Fragments arranged by size:
2889 1027 1013 530 360 314 245 141
62
NaeI GCC'GGC
Cuts at: 2922 4762 2922
Size: 1840 4741
NarI GG'CG_CC
Cuts at: 3166 4012 4260 3166
Size: 846 248 5487
Fragments arranged by size:
5487 846 248
NcoI C'CATG_G
Cuts at: 1676 1676
Size: 6581
NgoMIV G'CCGG_C
Cuts at: 2920 4760 2920
Size: 1840 4741
NheI G'CTAG_C
Cuts at: 1774 1774
Size: 6581
NspI r_CATG'y
Cuts at: 147 741 810 1753 2770 3634 4134 5041 147
Size: 594 69 943 1017 864 500 907 1687
Fragments arranged by size:
1687 1017 943 907 864 594 500 69
PasI CC'CwG_GG
Cuts at: 2757 2986 2757
Size: 229 6352
PciI A'CATG_T
Cuts at: 806 5037 806
Size: 4231 2350
PflMI CCAn_nnn'nTGG
Cuts at: 192 398 555 1524 2937 192
Size: 206 157 969 1413 3836
Fragments arranged by size:
3836 1413 969 206 157
PfoI T'CCnGG_A
Cuts at: 1421 2846 2975 3146 3320 1421
Size: 1425 129 171 174 4682
Fragments arranged by size:
4682 1425 174 171 129
PmeI GTTT'AAAC
Cuts at: 3580 3580
Size: 6581
PmlI CAC'GTG
Cuts at: 426 750 2676 3546 426
Size: 324 1926 870 3461
Fragments arranged by size:
3461 1926 870 324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 574 606 5720 5752 574
Size: 32 5114 32 1403
Fragments arranged by size:
5114 1403 32 32
PpuMI rG'GwC_Cy
Cuts at: 756 2073 756
Size: 1317 5264
PshAI GACnn'nnGTC
Cuts at: 1575 2065 1575
Size: 490 6091
PspOMI G'GGCC_C
Cuts at: 3292 3437 3292
Size: 145 6436
PstI C_TGCA'G
Cuts at: 488 770 785 2219 2543 3161 3176 3628 4313
Size: 282 15 1434 324 618 15 452 685
Cuts at: 4313 488
Size: 2756
Fragments arranged by size:
2756 1434 685 618 452 324 282 15
15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6581
PvuII CAG'CTG
Cuts at: 1932 3021 3162 4006 4366 1932
Size: 1089 141 844 360 4147
Fragments arranged by size:
4147 1089 844 360 141
RsrII CG'GwC_CG
Cuts at: 4776 4776
Size: 6581
SacI G_AGCT'C
Cuts at: 930 1307 3595 930
Size: 377 2288 3916
Fragments arranged by size:
3916 2288 377
SalI G'TCGA_C
Cuts at: 3618 3618
Size: 6581
SanDI GG'GwC_CC
Cuts at: 2073 2073
Size: 6581
SapI GCTCTTCn'nnn_
Cuts at: 1868 3023 4604 4814 1868
Size: 1155 1581 210 3635
Fragments arranged by size:
3635 1581 1155 210
SbfI CC_TGCA'GG
Cuts at: 770 2543 3628 770
Size: 1773 1085 3723
Fragments arranged by size:
3723 1773 1085
ScaI AGT'ACT
Cuts at: 1050 6098 1050
Size: 5048 1533
SfcI C'TryA_G
Cuts at: 33 294 484 766 781 1287 1440 1588 2215
Size: 261 190 282 15 506 153 148 627
Cuts at: 2215 2539 2667 3157 3172 3624 4309 5302 5493
Size: 324 128 490 15 452 685 993 191
Cuts at: 5493 33
Size: 1121
Fragments arranged by size:
1121 993 685 627 506 490 452 324
282 261 191 190 153 148 128 15
15
SfoI GGC'GCC
Cuts at: 3167 4013 4261 3167
Size: 846 248 5487
Fragments arranged by size:
5487 846 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6581
SgrAI Cr'CCGG_yG
Cuts at: 1716 2920 1716
Size: 1204 5377
SmaI CCC'GGG
Cuts at: 2723 3603 2723
Size: 880 5701
SmlI C'TyrA_G
Cuts at: 2424 3736 4651 5143 5405 2424
Size: 1312 915 492 262 3600
Fragments arranged by size:
3600 1312 915 492 262
SnaBI TAC'GTA
Cuts at: 3573 3573
Size: 6581
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6581
SphI G_CATG'C
Cuts at: 741 3634 4134 741
Size: 2893 500 3188
Fragments arranged by size:
3188 2893 500
SrfI GCCC'GGGC
Cuts at: 2723 2723
Size: 6581
SspI AAT'ATT
Cuts at: 2346 2346
Size: 6581
StuI AGG'CCT
Cuts at: 3050 3568 3050
Size: 518 6063
StyI C'CwwG_G
Cuts at: 392 729 1676 3715 4069 392
Size: 337 947 2039 354 2904
Fragments arranged by size:
2904 2039 947 354 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1385 4164 4297 4756 4965 1385
Size: 2779 133 459 209 3001
Fragments arranged by size:
3001 2779 459 209 133
TatI w'GTAC_w
Cuts at: 1048 6096 1048
Size: 5048 1533
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 856 888 1381 1413 1719 1751 2552 2584 6098
Size: 32 493 32 306 32 801 32 3514
Cuts at: 6098 6130 856
Size: 32 1307
Fragments arranged by size:
3514 1307 801 493 306 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3364 4378 3364
Size: 1014 5567
XbaI T'CTAG_A
Cuts at: 3612 3612
Size: 6581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1368 2305 4076 1368
Size: 937 1771 3873
Fragments arranged by size:
3873 1771 937
XmaI C'CCGG_G
Cuts at: 2721 3601 2721
Size: 880 5701
XmnI GAAnn'nnTTC
Cuts at: 217 1226 2195 217
Size: 1009 969 4603
Fragments arranged by size:
4603 1009 969
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BfrBI BsiWI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NotI NruI NsiI PacI PsiI
PspXI PsrI SacII SexAI SfiI SwaI XhoI ZraI