Restriction map for
pF1KSDA1886
(Circular) (Six-base) MAPSORT of: pF1KSDA1886.seq Check: 5767 from: 1 to: 5897
pF1KSDA1886 5897 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1490 2515 2935 1490
Size: 1025 420 4452
Fragments arranged by size:
4452 1025 420
Acc65I G'GTAC_C
Cuts at: 2913 2913
Size: 5897
AclI AA'CG_TT
Cuts at: 5737 5737
Size: 5897
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1681 1824 1939 2245 2489 2691 3722 4154
Size: 1250 143 115 306 244 202 1031 432
Cuts at: 4154 4901 5169 431
Size: 747 268 1159
Fragments arranged by size:
1250 1159 1031 747 432 306 268 244
202 143 115
AfeI AGC'GCT
Cuts at: 2230 2230
Size: 5897
AflIII A'CryG_T
Cuts at: 1283 3249 4353 5330 1283
Size: 1966 1104 977 1850
Fragments arranged by size:
1966 1850 1104 977
AgeI A'CCGG_T
Cuts at: 4285 4285
Size: 5897
AhdI GACnn_n'nnGTC
Cuts at: 916 1998 916
Size: 1082 4815
AleI CACnn'nnGTG
Cuts at: 256 2722 256
Size: 2466 3431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 422 1037 422
Size: 615 5282
AlwNI CAG_nnn'CTG
Cuts at: 390 574 601 660 711 861 903 960 1083
Size: 184 27 59 51 150 42 57 123
Cuts at: 1083 2341 3202 4769 390
Size: 1258 861 1567 1518
Fragments arranged by size:
1567 1518 1258 861 184 150 123 59
57 51 42 27
ApaI G_GGCC'C
Cuts at: 313 477 787 1874 2299 2613 2735 2830 313
Size: 164 310 1087 425 314 122 95 3380
Fragments arranged by size:
3380 1087 425 314 310 164 122 95
ApaLI G'TGCA_C
Cuts at: 1747 4667 1747
Size: 2920 2977
ApoI r'AATT_y
Cuts at: 2901 5250 5506 2901
Size: 2349 256 3292
Fragments arranged by size:
3292 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5897
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5897
AvaI C'yCGr_G
Cuts at: 507 728 821 1212 1958 2451 2917 507
Size: 221 93 391 746 493 466 3487
Fragments arranged by size:
3487 746 493 466 391 221 93
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 859 892 859
Size: 33 5864
BamHI G'GATC_C
Cuts at: 1943 2922 1943
Size: 979 4918
BanI G'GyrC_C
Cuts at: 418 1144 1849 1861 2541 2913 3327 3575 3610
Size: 726 705 12 680 372 414 248 35
Cuts at: 3610 418
Size: 2705
Fragments arranged by size:
2705 726 705 680 414 372 248 35
12
BanII G_rGCy'C
Cuts at: 313 477 787 1430 1874 2248 2299 2613 2735
Size: 164 310 643 444 374 51 314 122
Cuts at: 2735 2830 2911 3941 313
Size: 95 81 1030 2269
Fragments arranged by size:
2269 1030 643 444 374 314 310 164
122 95 81 51
BbeI G_GCGC'C
Cuts at: 422 1148 3331 3579 422
Size: 726 2183 248 2740
Fragments arranged by size:
2740 2183 726 248
BbsI GAAGACnn'nnnn_
Cuts at: 1461 1461
Size: 5897
BciVI GTATCCnnnnn_n'
Cuts at: 887 953 3795 4556 5897 887
Size: 66 2842 761 1341 887
Fragments arranged by size:
2842 1341 887 761 66
BclI T'GATC_A
Cuts at: 3417 3417
Size: 5897
BglI GCCn_nnn'nGGC
Cuts at: 461 512 628 791 1184 1679 2214 2758 461
Size: 51 116 163 393 495 535 544 3600
Fragments arranged by size:
3600 544 535 495 393 163 116 51
BglII A'GATC_T
Cuts at: 3412 5449 3412
Size: 2037 3860
BlpI GC'TnA_GC
Cuts at: 661 1808 3009 661
Size: 1147 1201 3549
Fragments arranged by size:
3549 1201 1147
Bme1580I G_kGCm'C
Cuts at: 313 477 787 1049 1751 1874 1927 2177 2299
Size: 164 310 262 702 123 53 250 122
Cuts at: 2299 2613 2735 2830 3522 3615 4671 313
Size: 314 122 95 692 93 1056 1539
Fragments arranged by size:
1539 1056 702 692 314 310 262 250
164 123 122 122 95 93 53
BmrI ACTGGGnnnn_n'
Cuts at: 987 1071 1217 3109 3190 3525 5669 987
Size: 84 146 1892 81 335 2144 1215
Fragments arranged by size:
2144 1892 1215 335 146 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4264 4264
Size: 5897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1444 1540 2017 2089 2098 2600 2768 622
Size: 822 96 477 72 9 502 168 3751
Fragments arranged by size:
3751 822 502 477 168 96 72 9
Bpu10I CC'TnA_GC
Cuts at: 2682 2682
Size: 5897
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 3073 3952 4444 4742 1312
Size: 1761 879 492 298 2467
Fragments arranged by size:
2467 1761 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5601 5601
Size: 5897
BsaAI yAC'GTr
Cuts at: 435 2156 2256 2889 3250 435
Size: 1721 100 633 361 3082
Fragments arranged by size:
3082 1721 633 361 100
BsaBI GATnn'nnATC
Cuts at: 6 2143 3411 5448 6
Size: 2137 1268 2037 455
Fragments arranged by size:
2137 2037 1268 455
BsaHI Gr'CG_yC
Cuts at: 419 1145 3328 3576 4278 5774 419
Size: 726 2183 248 702 1496 542
Fragments arranged by size:
2183 1496 726 702 542 248
BsaWI w'CCGG_w
Cuts at: 3086 3607 4285 4559 4706 5117 3086
Size: 521 678 274 147 411 3866
Fragments arranged by size:
3866 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1327 1357 2822 2852 1327
Size: 30 1465 30 4372
Fragments arranged by size:
4372 1465 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1774 2149 2218 1774
Size: 375 69 5453
Fragments arranged by size:
5453 375 69
BseYI C'CCAG_C
Cuts at: 896 982 1003 2097 2266 2347 2422 2488 2624
Size: 86 21 1094 169 81 75 66 136
Cuts at: 2624 2649 2737 3322 4080 4657 5153 896
Size: 25 88 585 758 577 496 1640
Fragments arranged by size:
1640 1094 758 585 577 496 169 136
88 86 81 75 66 25 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1606 2178 2614 457
Size: 1149 572 436 3740
Fragments arranged by size:
3740 1149 572 436
BsiEI CG_ry'CG
Cuts at: 104 3439 3485 4693 104
Size: 3335 46 1208 1308
Fragments arranged by size:
3335 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1751 1814 2261 2911 3689 3879 4671 1751
Size: 63 447 650 778 190 792 2977
Fragments arranged by size:
2977 792 778 650 447 190 63
Bsp1286I G_dGCh'C
Cuts at: 313 477 787 1049 1430 1751 1814 1874 1927
Size: 164 310 262 381 321 63 60 53
Cuts at: 1927 2177 2248 2261 2299 2613 2735 2830 2911
Size: 250 71 13 38 314 122 95 81
Cuts at: 2911 3522 3615 3689 3879 3941 4671 313
Size: 611 93 74 190 62 730 1539
Fragments arranged by size:
1539 730 611 381 321 314 310 262
250 190 164 122 95 93 81 74
71 63 62 60 53 38 13
BspEI T'CCGG_A
Cuts at: 5117 5117
Size: 5897
BspHI T'CATG_A
Cuts at: 1965 5073 1965
Size: 3108 2789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 159 1578 2015 2539 2789 2870 2947 3161 3463
Size: 1419 437 524 250 81 77 214 302
Cuts at: 3463 3844 159
Size: 381 2212
Fragments arranged by size:
2212 1419 524 437 381 302 250 214
81 77
BsrBI CCG'CTC
Cuts at: 50 1611 1766 2025 4189 4243 5727 5892 50
Size: 1561 155 259 2164 54 1484 165 55
Fragments arranged by size:
2164 1561 1484 259 165 155 55 54
BsrDI GCAATG_nn'
Cuts at: 3809 3809
Size: 5897
BsrFI r'CCGG_y
Cuts at: 228 1553 3895 4076 4285 228
Size: 1325 2342 181 209 1840
Fragments arranged by size:
2342 1840 1325 209 181
BsrGI T'GTAC_A
Cuts at: 449 449
Size: 5897
BssSI C'ACGA_G
Cuts at: 1546 4168 4526 1546
Size: 2622 358 2917
Fragments arranged by size:
2917 2622 358
BstAPI GCAn_nnn'nTGC
Cuts at: 574 601 861 960 1053 1746 3274 574
Size: 27 260 99 93 693 1528 3197
Fragments arranged by size:
3197 1528 693 260 99 93 27
BstBI TT'CG_AA
Cuts at: 110 4258 110
Size: 4148 1749
BstEII G'GTnAC_C
Cuts at: 2551 2551
Size: 5897
BstXI CCAn_nnnn'nTGG
Cuts at: 2274 2379 2274
Size: 105 5792
BstYI r'GATC_y
Cuts at: 1 1943 2922 3389 3412 3747 3993 5005 5091
Size: 1942 979 467 23 335 246 1012 86
Cuts at: 5091 5103 5449 1
Size: 12 346 449
Fragments arranged by size:
1942 1012 979 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 305 456 626 1100 305
Size: 151 170 474 5102
Fragments arranged by size:
5102 474 170 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 691 1340 1424 3847 4028 4191 691
Size: 649 84 2423 181 163 2397
Fragments arranged by size:
2423 2397 649 181 163 84
BtsI GCAGTG_nn'
Cuts at: 599 901 1231 1812 3140 599
Size: 302 330 581 1328 3356
Fragments arranged by size:
3356 1328 581 330 302
DraI TTT'AAA
Cuts at: 2896 2896
Size: 5897
DraIII CAC_nnn'GTG
Cuts at: 175 175
Size: 5897
DrdI GACnn_nn'nnGTC
Cuts at: 3603 4461 3603
Size: 858 5039
EaeI y'GGCC_r
Cuts at: 1573 2361 3482 3656 4047 4074 5419 1573
Size: 788 1121 174 391 27 1345 2051
Fragments arranged by size:
2051 1345 1121 788 391 174 27
EagI C'GGCC_G
Cuts at: 3482 3482
Size: 5897
EarI CTCTTCn'nnn_
Cuts at: 1735 3920 4130 1735
Size: 2185 210 3502
Fragments arranged by size:
3502 2185 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 357 1423 4105 4415 4561 5707 357
Size: 1066 2682 310 146 1146 547
Fragments arranged by size:
2682 1146 1066 547 310 146
EcoICRI GAG'CTC
Cuts at: 2909 2909
Size: 5897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 622 1444 1540 1681 1824 1939 2017 2089
Size: 191 822 96 141 143 115 78 72
Cuts at: 2089 2098 2245 2489 2600 2691 2768 3722 4154
Size: 9 147 244 111 91 77 954 432
Cuts at: 4154 4901 5169 431
Size: 747 268 1159
Fragments arranged by size:
1159 954 822 747 432 268 244 191
147 143 141 115 111 96 91 78
77 72 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1063 1938 1958 2717 1063
Size: 875 20 759 4243
Fragments arranged by size:
4243 875 759 20
EcoO109I rG'GnC_Cy
Cuts at: 310 473 474 513 724 733 784 1779 2073
Size: 163 1 39 211 9 51 995 294
Cuts at: 2073 2296 2383 2609 2610 2620 2686 2731 2732
Size: 223 87 226 1 10 66 45 1
Cuts at: 2732 2760 3036 310
Size: 28 276 3171
Fragments arranged by size:
3171 995 294 276 226 223 211 163
87 66 51 45 39 28 10 9
1 1 1
EcoRI G'AATT_C
Cuts at: 2901 2901
Size: 5897
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1329 1391 1854 3916 1329
Size: 62 463 2062 3310
Fragments arranged by size:
3310 2062 463 62
FspI TGC'GCA
Cuts at: 3678 3678
Size: 5897
HaeII r_GCGC'y
Cuts at: 221 422 1148 1797 2232 2306 3331 3579 4601
Size: 201 726 649 435 74 1025 248 1022
Cuts at: 4601 5580 221
Size: 979 538
Fragments arranged by size:
1025 1022 979 726 649 538 435 248
201 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 902 934 1291 1323 1472 1504 1984 2016 2800
Size: 32 357 32 149 32 480 32 784
Cuts at: 2800 2832 3739 3769 3771 3801 902
Size: 32 907 30 2 30 2998
Fragments arranged by size:
2998 907 784 480 357 149 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2936 2936
Size: 5897
KasI G'GCGC_C
Cuts at: 418 1144 3327 3575 418
Size: 726 2183 248 2740
Fragments arranged by size:
2740 2183 726 248
KpnI G_GTAC'C
Cuts at: 2917 2917
Size: 5897
MluI A'CGCG_T
Cuts at: 5330 5330
Size: 5897
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2624 4568 4752 2624
Size: 1944 184 3769
Fragments arranged by size:
3769 1944 184
MscI TGG'CCA
Cuts at: 1575 2363 3658 1575
Size: 788 1295 3814
Fragments arranged by size:
3814 1295 788
MslI CAynn'nnrTG
Cuts at: 176 256 2722 3886 4013 176
Size: 80 2466 1164 127 2060
Fragments arranged by size:
2466 2060 1164 127 80
MspA1I CmG'CkG
Cuts at: 458 571 580 748 1476 1847 2162 2628 3008
Size: 113 9 168 728 371 315 466 380
Cuts at: 3008 3322 3682 4695 4940 458
Size: 314 360 1013 245 1415
Fragments arranged by size:
1415 1013 728 466 380 371 360 315
314 245 168 113 9
NaeI GCC'GGC
Cuts at: 230 4078 230
Size: 3848 2049
NarI GG'CG_CC
Cuts at: 419 1145 3328 3576 419
Size: 726 2183 248 2740
Fragments arranged by size:
2740 2183 726 248
NcoI C'CATG_G
Cuts at: 305 305
Size: 5897
NdeI CA'TA_TG
Cuts at: 192 192
Size: 5897
NgoMIV G'CCGG_C
Cuts at: 228 4076 228
Size: 3848 2049
NspI r_CATG'y
Cuts at: 535 1287 1669 2097 2193 2745 2950 3450 4357
Size: 752 382 428 96 552 205 500 907
Cuts at: 4357 535
Size: 2075
Fragments arranged by size:
2075 907 752 552 500 428 382 205
96
PasI CC'CwG_GG
Cuts at: 469 1009 1042 1867 2292 469
Size: 540 33 825 425 4074
Fragments arranged by size:
4074 825 540 425 33
PciI A'CATG_T
Cuts at: 1283 4353 1283
Size: 3070 2827
PflMI CCAn_nnn'nTGG
Cuts at: 294 294
Size: 5897
PfoI T'CCnGG_A
Cuts at: 638 1421 2786 638
Size: 783 1365 3749
Fragments arranged by size:
3749 1365 783
PmeI GTTT'AAAC
Cuts at: 2896 2896
Size: 5897
PmlI CAC'GTG
Cuts at: 2156 2256 2156
Size: 100 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 5036
Size: 32 5865
PpuMI rG'GwC_Cy
Cuts at: 724 2620 2686 724
Size: 1896 66 3935
Fragments arranged by size:
3935 1896 66
PsiI TTA'TAA
Cuts at: 1115 1115
Size: 5897
PspOMI G'GGCC_C
Cuts at: 309 473 783 1870 2295 2609 2731 2826 309
Size: 164 310 1087 425 314 122 95 3380
Fragments arranged by size:
3380 1087 425 314 310 164 122 95
PstI C_TGCA'G
Cuts at: 318 579 716 1064 1442 1742 1778 2012 2327
Size: 261 137 348 378 300 36 234 315
Cuts at: 2327 2944 3629 318
Size: 617 685 2586
Fragments arranged by size:
2586 685 617 378 348 315 300 261
234 137 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5897
PvuII CAG'CTG
Cuts at: 571 580 1476 2162 2628 3322 3682 571
Size: 9 896 686 466 694 360 2786
Fragments arranged by size:
2786 896 694 686 466 360 9
RsrII CG'GwC_CG
Cuts at: 4092 4092
Size: 5897
SacI G_AGCT'C
Cuts at: 2911 2911
Size: 5897
SacII CC_GC'GG
Cuts at: 459 459
Size: 5897
SalI G'TCGA_C
Cuts at: 2934 2934
Size: 5897
SapI GCTCTTCn'nnn_
Cuts at: 3920 4130 3920
Size: 210 5687
SbfI CC_TGCA'GG
Cuts at: 318 2944 318
Size: 2626 3271
ScaI AGT'ACT
Cuts at: 5414 5414
Size: 5897
SfcI C'TryA_G
Cuts at: 33 314 575 712 1060 1173 1438 1738 1774
Size: 281 261 137 348 113 265 300 36
Cuts at: 1774 2008 2323 2940 3625 4618 4809 33
Size: 234 315 617 685 993 191 1121
Fragments arranged by size:
1121 993 685 617 348 315 300 281
265 261 234 191 137 113 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 512 791 512
Size: 279 5618
SfoI GGC'GCC
Cuts at: 420 1146 3329 3577 420
Size: 726 2183 248 2740
Fragments arranged by size:
2740 2183 726 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5897
SmaI CCC'GGG
Cuts at: 509 730 823 1214 2453 2919 509
Size: 221 93 391 1239 466 3487
Fragments arranged by size:
3487 1239 466 391 221 93
SmlI C'TyrA_G
Cuts at: 1327 3052 3967 4459 4721 1327
Size: 1725 915 492 262 2503
Fragments arranged by size:
2503 1725 915 492 262
SnaBI TAC'GTA
Cuts at: 2889 2889
Size: 5897
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5897
SphI G_CATG'C
Cuts at: 2950 3450 2950
Size: 500 5397
StuI AGG'CCT
Cuts at: 1512 1512
Size: 5897
StyI C'CwwG_G
Cuts at: 205 305 803 2604 2666 3031 3385 205
Size: 100 498 1801 62 365 354 2717
Fragments arranged by size:
2717 1801 498 365 354 100 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 996 1269 2427 3480 3613 4072 4281 996
Size: 273 1158 1053 133 459 209 2612
Fragments arranged by size:
2612 1158 1053 459 273 209 133
TatI w'GTAC_w
Cuts at: 449 2090 5412 449
Size: 1641 3322 934
Fragments arranged by size:
3322 1641 934
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1741 1773 2556 2588 2822 2854 5414 5446 1741
Size: 32 783 32 234 32 2560 32 2192
Fragments arranged by size:
2560 2192 783 234 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1077 1486 3694 1077
Size: 409 2208 3280
Fragments arranged by size:
3280 2208 409
XbaI T'CTAG_A
Cuts at: 2928 2928
Size: 5897
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 624 2724 3392 624
Size: 2100 668 3129
Fragments arranged by size:
3129 2100 668
XmaI C'CCGG_G
Cuts at: 507 728 821 1212 2451 2917 507
Size: 221 93 391 1239 466 3487
Fragments arranged by size:
3487 1239 466 391 221 93
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AloI AscI AvrII BbvCI BcgI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BssHII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PshAI PspXI
PsrI SanDI SexAI SgrAI SrfI SspI SwaI XhoI
XmnI ZraI