Restriction map for
pF1KSDA1895
(Circular) (Six-base) MAPSORT of: pF1KSDA1895.seq Check: 5865 from: 1 to: 4970
pF1KSDA1895 4970 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1246 1246
Size: 4970
AccI GT'mk_AC
Cuts at: 1669 2008 1669
Size: 339 4631
Acc65I G'GTAC_C
Cuts at: 1986 1986
Size: 4970
AclI AA'CG_TT
Cuts at: 1123 4810 1123
Size: 3687 1283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 1480 2795 3227 3974 4242 590
Size: 890 1315 432 747 268 1318
Fragments arranged by size:
1318 1315 890 747 432 268
AflII C'TTAA_G
Cuts at: 406 406
Size: 4970
AflIII A'CryG_T
Cuts at: 1623 2322 3426 4403 1623
Size: 699 1104 977 2190
Fragments arranged by size:
2190 1104 977 699
AgeI A'CCGG_T
Cuts at: 3358 3358
Size: 4970
AleI CACnn'nnGTG
Cuts at: 994 994
Size: 4970
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1589 1589
Size: 4970
AlwNI CAG_nnn'CTG
Cuts at: 2275 3842 2275
Size: 1567 3403
ApaLI G'TGCA_C
Cuts at: 3740 3740
Size: 4970
ApoI r'AATT_y
Cuts at: 673 1143 1729 1974 4323 4579 673
Size: 470 586 245 2349 256 1064
Fragments arranged by size:
2349 1064 586 470 256 245
AseI AT'TA_AT
Cuts at: 20 20
Size: 4970
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
AvaI C'yCGr_G
Cuts at: 1990 1990
Size: 4970
BamHI G'GATC_C
Cuts at: 1995 1995
Size: 4970
BanI G'GyrC_C
Cuts at: 1986 2400 2648 2683 1986
Size: 414 248 35 4273
Fragments arranged by size:
4273 414 248 35
BanII G_rGCy'C
Cuts at: 1984 3014 1984
Size: 1030 3940
BbeI G_GCGC'C
Cuts at: 2404 2652 2404
Size: 248 4722
BbsI GAAGACnn'nnnn_
Cuts at: 335 335
Size: 4970
BciVI GTATCCnnnnn_n'
Cuts at: 2868 3629 4970 2868
Size: 761 1341 2868
Fragments arranged by size:
2868 1341 761
BclI T'GATC_A
Cuts at: 2490 2490
Size: 4970
BfrBI ATG'CAT
Cuts at: 1791 1791
Size: 4970
BglII A'GATC_T
Cuts at: 2485 4522 2485
Size: 2037 2933
BlpI GC'TnA_GC
Cuts at: 2082 2082
Size: 4970
Bme1580I G_kGCm'C
Cuts at: 2595 2688 3744 2595
Size: 93 1056 3821
Fragments arranged by size:
3821 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 941 1022 2182 2263 2598 4742 941
Size: 81 1160 81 335 2144 1169
Fragments arranged by size:
2144 1169 1160 335 81 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1866 3337 1866
Size: 1471 3499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 850 442
Size: 408 4562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2146 3025 3517 3815 2146
Size: 879 492 298 3301
Fragments arranged by size:
3301 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 213 260 4674 213
Size: 47 4414 509
Fragments arranged by size:
4414 509 47
BsaAI yAC'GTr
Cuts at: 1962 2323 1962
Size: 361 4609
BsaBI GATnn'nnATC
Cuts at: 6 2484 4521 6
Size: 2478 2037 455
Fragments arranged by size:
2478 2037 455
BsaHI Gr'CG_yC
Cuts at: 2401 2649 3351 4847 2401
Size: 248 702 1496 2524
Fragments arranged by size:
2524 1496 702 248
BsaWI w'CCGG_w
Cuts at: 204 2159 2680 3358 3632 3779 4190 204
Size: 1955 521 678 274 147 411 984
Fragments arranged by size:
1955 984 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 278 278
Size: 4970
BseYI C'CCAG_C
Cuts at: 884 2395 3153 3730 4226 884
Size: 1511 758 577 496 1628
Fragments arranged by size:
1628 1511 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 971 971
Size: 4970
BsiEI CG_ry'CG
Cuts at: 104 2512 2558 3766 104
Size: 2408 46 1208 1308
Fragments arranged by size:
2408 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 194 1984 2762 2952 3744 194
Size: 1790 778 190 792 1420
Fragments arranged by size:
1790 1420 792 778 190
BsmI GAATG_Cn'
Cuts at: 554 554
Size: 4970
BsmBI CGTCTCn'nnnn_
Cuts at: 1186 1295 1186
Size: 109 4861
Bsp1286I G_dGCh'C
Cuts at: 194 1984 2595 2688 2762 2952 3014 3744 194
Size: 1790 611 93 74 190 62 730 1420
Fragments arranged by size:
1790 1420 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4190 4190
Size: 4970
BspHI T'CATG_A
Cuts at: 4146 4146
Size: 4970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1246 2020 2234 2536 2917 1246
Size: 774 214 302 381 3299
Fragments arranged by size:
3299 774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3262 3316 4800 4965 50
Size: 3212 54 1484 165 55
Fragments arranged by size:
3212 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2882 2882
Size: 4970
BsrFI r'CCGG_y
Cuts at: 2968 3149 3358 2968
Size: 181 209 4580
Fragments arranged by size:
4580 209 181
BssSI C'ACGA_G
Cuts at: 3241 3599 3241
Size: 358 4612
BstAPI GCAn_nnn'nTGC
Cuts at: 2347 2347
Size: 4970
BstBI TT'CG_AA
Cuts at: 110 3331 110
Size: 3221 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 637 1322 1552 637
Size: 685 230 4055
Fragments arranged by size:
4055 685 230
BstYI r'GATC_y
Cuts at: 1 1995 2462 2485 2820 3066 4078 4164 4176
Size: 1994 467 23 335 246 1012 86 12
Cuts at: 4176 4522 1
Size: 346 449
Fragments arranged by size:
1994 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1670 1670
Size: 4970
Bsu36I CC'TnA_GG
Cuts at: 1592 1592
Size: 4970
BtgI C'CryG_G
Cuts at: 1856 1897 1856
Size: 41 4929
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2920 3101 3264 2920
Size: 181 163 4626
Fragments arranged by size:
4626 181 163
BtsI GCAGTG_nn'
Cuts at: 2213 2213
Size: 4970
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1224 1259 1224
Size: 35 4935
DraI TTT'AAA
Cuts at: 1251 1969 1251
Size: 718 4252
DraIII CAC_nnn'GTG
Cuts at: 421 1522 421
Size: 1101 3869
DrdI GACnn_nn'nnGTC
Cuts at: 1447 2676 3534 1447
Size: 1229 858 2883
Fragments arranged by size:
2883 1229 858
EaeI y'GGCC_r
Cuts at: 576 1542 1784 2555 2729 3120 3147 4492 576
Size: 966 242 771 174 391 27 1345 1054
Fragments arranged by size:
1345 1054 966 771 391 242 174 27
EagI C'GGCC_G
Cuts at: 2555 2555
Size: 4970
EarI CTCTTCn'nnn_
Cuts at: 1364 1427 2993 3203 1364
Size: 63 1566 210 3131
Fragments arranged by size:
3131 1566 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3178 3488 3634 4780 3178
Size: 310 146 1146 3368
Fragments arranged by size:
3368 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1982 1982
Size: 4970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 590 850 1480 2795 3227 3974 4242 442
Size: 148 260 630 1315 432 747 268 1170
Fragments arranged by size:
1315 1170 747 630 432 268 260 148
EcoO109I rG'GnC_Cy
Cuts at: 2109 2109
Size: 4970
EcoRI G'AATT_C
Cuts at: 1974 1974
Size: 4970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 520 2989 520
Size: 2469 2501
FspI TGC'GCA
Cuts at: 2751 2751
Size: 4970
HaeII r_GCGC'y
Cuts at: 2404 2652 3674 4653 2404
Size: 248 1022 979 2721
Fragments arranged by size:
2721 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 468 500 640 672 770 802 995 1027 2812
Size: 32 140 32 98 32 193 32 1785
Cuts at: 2812 2842 2844 2874 468
Size: 30 2 30 2564
Fragments arranged by size:
2564 1785 193 140 98 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2009 2009
Size: 4970
HindIII A'AGCT_T
Cuts at: 733 733
Size: 4970
KasI G'GCGC_C
Cuts at: 2400 2648 2400
Size: 248 4722
KpnI G_GTAC'C
Cuts at: 1990 1990
Size: 4970
MfeI C'AATT_G
Cuts at: 689 689
Size: 4970
MluI A'CGCG_T
Cuts at: 4403 4403
Size: 4970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3641 3825 3641
Size: 184 4786
MscI TGG'CCA
Cuts at: 578 1544 1786 2731 578
Size: 966 242 945 2817
Fragments arranged by size:
2817 966 945 242
MslI CAynn'nnrTG
Cuts at: 994 1550 2959 3086 994
Size: 556 1409 127 2878
Fragments arranged by size:
2878 1409 556 127
MspA1I CmG'CkG
Cuts at: 2081 2395 2755 3768 4013 2081
Size: 314 360 1013 245 3038
Fragments arranged by size:
3038 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3151 3151
Size: 4970
NarI GG'CG_CC
Cuts at: 2401 2649 2401
Size: 248 4722
NcoI C'CATG_G
Cuts at: 1856 1897 1856
Size: 41 4929
NdeI CA'TA_TG
Cuts at: 1551 1664 1551
Size: 113 4857
NgoMIV G'CCGG_C
Cuts at: 3149 3149
Size: 4970
NsiI A_TGCA'T
Cuts at: 1793 1793
Size: 4970
NspI r_CATG'y
Cuts at: 1282 1795 2023 2523 3430 1282
Size: 513 228 500 907 2822
Fragments arranged by size:
2822 907 513 500 228
PciI A'CATG_T
Cuts at: 3426 3426
Size: 4970
PflMI CCAn_nnn'nTGG
Cuts at: 1522 1522
Size: 4970
PmeI GTTT'AAAC
Cuts at: 1969 1969
Size: 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 4109
Size: 32 4938
PstI C_TGCA'G
Cuts at: 2017 2702 2017
Size: 685 4285
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4970
PvuII CAG'CTG
Cuts at: 2395 2755 2395
Size: 360 4610
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 4970
SacI G_AGCT'C
Cuts at: 1984 1984
Size: 4970
SalI G'TCGA_C
Cuts at: 2007 2007
Size: 4970
SapI GCTCTTCn'nnn_
Cuts at: 1364 1427 2993 3203 1364
Size: 63 1566 210 3131
Fragments arranged by size:
3131 1566 210 63
SbfI CC_TGCA'GG
Cuts at: 2017 2017
Size: 4970
ScaI AGT'ACT
Cuts at: 237 4487 237
Size: 4250 720
SfcI C'TryA_G
Cuts at: 33 2013 2698 3691 3882 33
Size: 1980 685 993 191 1121
Fragments arranged by size:
1980 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2402 2650 2402
Size: 248 4722
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
SmaI CCC'GGG
Cuts at: 1992 1992
Size: 4970
SmlI C'TyrA_G
Cuts at: 406 2125 3040 3532 3794 406
Size: 1719 915 492 262 1582
Fragments arranged by size:
1719 1582 915 492 262
SnaBI TAC'GTA
Cuts at: 1962 1962
Size: 4970
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4970
SphI G_CATG'C
Cuts at: 1795 2023 2523 1795
Size: 228 500 4242
Fragments arranged by size:
4242 500 228
StyI C'CwwG_G
Cuts at: 489 543 1411 1856 1897 2104 2458 489
Size: 54 868 445 41 207 354 3001
Fragments arranged by size:
3001 868 445 354 207 54 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 898 2553 2686 3145 3354 898
Size: 1655 133 459 209 2514
Fragments arranged by size:
2514 1655 459 209 133
TatI w'GTAC_w
Cuts at: 235 533 4485 235
Size: 298 3952 720
Fragments arranged by size:
3952 720 298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 784 816 4487 4519 784
Size: 32 3671 32 1235
Fragments arranged by size:
3671 1235 32 32
Tth111I GACn'n_nGTC
Cuts at: 2767 2767
Size: 4970
XbaI T'CTAG_A
Cuts at: 259 2001 259
Size: 1742 3228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2465 2465
Size: 4970
XmaI C'CCGG_G
Cuts at: 1990 1990
Size: 4970
XmnI GAAnn'nnTTC
Cuts at: 1625 1625
Size: 4970
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI BsiWI
BsrGI BssHII BstEII ClaI EcoNI EcoRV FseI FspAI
HpaI NheI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
ZraI