Restriction map for
pF1KSDA1896
(Circular) (Six-base) MAPSORT of: pF1KSDA1896.seq Check: 348 from: 1 to: 4640
pF1KSDA1896 4640 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1289 1589 1678 1289
Size: 300 89 4251
Fragments arranged by size:
4251 300 89
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 4480 4480
Size: 4640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1621 2465 2897 3644 3912 1153
Size: 468 844 432 747 268 1881
Fragments arranged by size:
1881 844 747 468 432 268
AflIII A'CryG_T
Cuts at: 1992 3096 4073 1992
Size: 1104 977 2559
Fragments arranged by size:
2559 1104 977
AgeI A'CCGG_T
Cuts at: 3028 3028
Size: 4640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 887 1340 887
Size: 453 4187
AlwNI CAG_nnn'CTG
Cuts at: 138 229 504 827 1388 1945 3512 138
Size: 91 275 323 561 557 1567 1266
Fragments arranged by size:
1567 1266 561 557 323 275 91
ApaI G_GGCC'C
Cuts at: 586 586
Size: 4640
ApaLI G'TGCA_C
Cuts at: 3410 3410
Size: 4640
ApoI r'AATT_y
Cuts at: 397 548 1000 1644 3993 4249 397
Size: 151 452 644 2349 256 788
Fragments arranged by size:
2349 788 644 452 256 151
AseI AT'TA_AT
Cuts at: 20 20
Size: 4640
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 676 1660 676
Size: 984 3656
BamHI G'GATC_C
Cuts at: 1196 1665 1196
Size: 469 4171
BanI G'GyrC_C
Cuts at: 176 217 1372 1656 2070 2318 2353 176
Size: 41 1155 284 414 248 35 2463
Fragments arranged by size:
2463 1155 414 284 248 41 35
BanII G_rGCy'C
Cuts at: 586 1654 2684 586
Size: 1068 1030 2542
Fragments arranged by size:
2542 1068 1030
BbeI G_GCGC'C
Cuts at: 2074 2322 2074
Size: 248 4392
BbsI GAAGACnn'nnnn_
Cuts at: 1141 1162 1141
Size: 21 4619
BbvCI CC'TCA_GC
Cuts at: 281 433 281
Size: 152 4488
BciVI GTATCCnnnnn_n'
Cuts at: 828 2538 3299 4640 828
Size: 1710 761 1341 828
Fragments arranged by size:
1710 1341 828 761
BclI T'GATC_A
Cuts at: 2160 2160
Size: 4640
BglII A'GATC_T
Cuts at: 2155 4192 2155
Size: 2037 2603
BlpI GC'TnA_GC
Cuts at: 1752 1752
Size: 4640
Bme1580I G_kGCm'C
Cuts at: 586 2265 2358 3414 586
Size: 1679 93 1056 1812
Fragments arranged by size:
1812 1679 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1852 1933 2268 4412 1852
Size: 81 335 2144 2080
Fragments arranged by size:
2144 2080 335 81
BmtI G_CTAG'C
Cuts at: 158 158
Size: 4640
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 255 380 171
Size: 84 125 4431
Fragments arranged by size:
4431 125 84
Bpu10I CC'TnA_GC
Cuts at: 281 433 281
Size: 152 4488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 835 1285 1816 2695 3187 3485 538
Size: 297 450 531 879 492 298 1693
Fragments arranged by size:
1693 879 531 492 450 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 233 4344 233
Size: 4111 529
BsaAI yAC'GTr
Cuts at: 1584 1632 1993 1584
Size: 48 361 4231
Fragments arranged by size:
4231 361 48
BsaBI GATnn'nnATC
Cuts at: 6 619 2154 4191 6
Size: 613 1535 2037 455
Fragments arranged by size:
2037 1535 613 455
BsaHI Gr'CG_yC
Cuts at: 2071 2319 3021 4517 2071
Size: 248 702 1496 2194
Fragments arranged by size:
2194 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1464 1829 2350 3028 3302 3449 3860 1464
Size: 365 521 678 274 147 411 2244
Fragments arranged by size:
2244 678 521 411 365 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 643 772 643
Size: 129 4511
BseYI C'CCAG_C
Cuts at: 172 222 1251 1563 2065 2823 3400 3896 172
Size: 50 1029 312 502 758 577 496 916
Fragments arranged by size:
1029 916 758 577 502 496 312 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 1639 813
Size: 826 3814
BsiEI CG_ry'CG
Cuts at: 104 204 2182 2228 3436 104
Size: 100 1978 46 1208 1308
Fragments arranged by size:
1978 1308 1208 100 46
BsiHKAI G_wGCw'C
Cuts at: 860 1361 1654 2432 2622 3414 860
Size: 501 293 778 190 792 2086
Fragments arranged by size:
2086 792 778 501 293 190
BsmI GAATG_Cn'
Cuts at: 699 1181 1311 699
Size: 482 130 4028
Fragments arranged by size:
4028 482 130
BsmBI CGTCTCn'nnnn_
Cuts at: 1288 1288
Size: 4640
Bsp1286I G_dGCh'C
Cuts at: 586 860 1361 1654 2265 2358 2432 2622 2684
Size: 274 501 293 611 93 74 190 62
Cuts at: 2684 3414 586
Size: 730 1812
Fragments arranged by size:
1812 730 611 501 293 274 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1464 3860 1464
Size: 2396 2244
BspHI T'CATG_A
Cuts at: 420 1550 3816 420
Size: 1130 2266 1244
Fragments arranged by size:
2266 1244 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 333 835 855 1690 1904 2206 2587 333
Size: 502 20 835 214 302 381 2386
Fragments arranged by size:
2386 835 502 381 302 214 20
BsrBI CCG'CTC
Cuts at: 50 2932 2986 4470 4635 50
Size: 2882 54 1484 165 55
Fragments arranged by size:
2882 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 963 2552 963
Size: 1589 3051
BsrFI r'CCGG_y
Cuts at: 1270 2638 2819 3028 1270
Size: 1368 181 209 2882
Fragments arranged by size:
2882 1368 209 181
BsrGI T'GTAC_A
Cuts at: 351 1526 351
Size: 1175 3465
BssSI C'ACGA_G
Cuts at: 1063 2911 3269 1063
Size: 1848 358 2434
Fragments arranged by size:
2434 1848 358
BstAPI GCAn_nnn'nTGC
Cuts at: 504 2017 504
Size: 1513 3127
BstBI TT'CG_AA
Cuts at: 110 3001 110
Size: 2891 1749
BstEII G'GTnAC_C
Cuts at: 1470 1470
Size: 4640
BstXI CCAn_nnnn'nTGG
Cuts at: 312 1251 312
Size: 939 3701
BstYI r'GATC_y
Cuts at: 1 1196 1665 2132 2155 2490 2736 3748 3834
Size: 1195 469 467 23 335 246 1012 86
Cuts at: 3834 3846 4192 1
Size: 12 346 449
Fragments arranged by size:
1195 1012 469 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 180 287 662 180
Size: 107 375 4158
Fragments arranged by size:
4158 375 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1083 2590 2771 2934 1083
Size: 1507 181 163 2789
Fragments arranged by size:
2789 1507 181 163
BtsI GCAGTG_nn'
Cuts at: 1334 1883 1334
Size: 549 4091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 693 728 693
Size: 35 4605
DraI TTT'AAA
Cuts at: 1639 1639
Size: 4640
DraIII CAC_nnn'GTG
Cuts at: 663 792 663
Size: 129 4511
DrdI GACnn_nn'nnGTC
Cuts at: 517 1285 2346 3204 517
Size: 768 1061 858 1953
Fragments arranged by size:
1953 1061 858 768
EaeI y'GGCC_r
Cuts at: 1202 1217 1392 1400 2225 2399 2790 2817 4162
Size: 15 175 8 825 174 391 27 1345
Cuts at: 4162 1202
Size: 1680
Fragments arranged by size:
1680 1345 825 391 175 174 27 15
8
EagI C'GGCC_G
Cuts at: 2225 2225
Size: 4640
EarI CTCTTCn'nnn_
Cuts at: 1492 2663 2873 1492
Size: 1171 210 3259
Fragments arranged by size:
3259 1171 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2848 3158 3304 4450 2848
Size: 310 146 1146 3038
Fragments arranged by size:
3038 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1652 1652
Size: 4640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 255 380 1153 1621 2465 2897 3644 3912
Size: 84 125 773 468 844 432 747 268
Cuts at: 3912 171
Size: 899
Fragments arranged by size:
899 844 773 747 468 432 268 125
84
EcoO109I rG'GnC_Cy
Cuts at: 369 582 583 1082 1128 1427 1514 1779 369
Size: 213 1 499 46 299 87 265 3230
Fragments arranged by size:
3230 499 299 265 213 87 46 1
EcoRI G'AATT_C
Cuts at: 1644 1644
Size: 4640
EcoRV GAT'ATC
Cuts at: 527 527
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 4640
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 1029 1150 1463 2074 2322 3344 4323 1029
Size: 121 313 611 248 1022 979 1346
Fragments arranged by size:
1346 1022 979 611 313 248 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2482 2512 2514 2544 2482
Size: 30 2 30 4578
Fragments arranged by size:
4578 30 30 2
HincII GTy'rAC
Cuts at: 1679 1679
Size: 4640
HindIII A'AGCT_T
Cuts at: 252 252
Size: 4640
KasI G'GCGC_C
Cuts at: 2070 2318 2070
Size: 248 4392
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 300 3311 3495 300
Size: 3011 184 1445
Fragments arranged by size:
3011 1445 184
MscI TGG'CCA
Cuts at: 1204 1394 1402 2401 1204
Size: 190 8 999 3443
Fragments arranged by size:
3443 999 190 8
MslI CAynn'nnrTG
Cuts at: 595 916 2629 2756 595
Size: 321 1713 127 2479
Fragments arranged by size:
2479 1713 321 127
MspA1I CmG'CkG
Cuts at: 1398 1567 1751 2065 2425 3438 3683 1398
Size: 169 184 314 360 1013 245 2355
Fragments arranged by size:
2355 1013 360 314 245 184 169
NaeI GCC'GGC
Cuts at: 1272 2821 1272
Size: 1549 3091
NarI GG'CG_CC
Cuts at: 2071 2319 2071
Size: 248 4392
NgoMIV G'CCGG_C
Cuts at: 1270 2819 1270
Size: 1549 3091
NheI G'CTAG_C
Cuts at: 154 154
Size: 4640
NspI r_CATG'y
Cuts at: 1251 1693 2193 3100 1251
Size: 442 500 907 2791
Fragments arranged by size:
2791 907 500 442
PasI CC'CwG_GG
Cuts at: 167 1611 167
Size: 1444 3196
PciI A'CATG_T
Cuts at: 3096 3096
Size: 4640
PflMI CCAn_nnn'nTGG
Cuts at: 626 663 626
Size: 37 4603
PfoI T'CCnGG_A
Cuts at: 737 737
Size: 4640
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 4608
PpuMI rG'GwC_Cy
Cuts at: 369 1082 1128 1427 369
Size: 713 46 299 3582
Fragments arranged by size:
3582 713 299 46
PspOMI G'GGCC_C
Cuts at: 582 582
Size: 4640
PstI C_TGCA'G
Cuts at: 330 1319 1687 2372 330
Size: 989 368 685 2598
Fragments arranged by size:
2598 989 685 368
PvuI CG_AT'CG
Cuts at: 104 204 104
Size: 100 4540
PvuII CAG'CTG
Cuts at: 1398 1567 2065 2425 1398
Size: 169 498 360 3613
Fragments arranged by size:
3613 498 360 169
RsrII CG'GwC_CG
Cuts at: 1505 2835 1505
Size: 1330 3310
SacI G_AGCT'C
Cuts at: 1654 1654
Size: 4640
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 2663 2873 2663
Size: 210 4430
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 1168 4157 1168
Size: 2989 1651
SexAI A'CCwGG_T
Cuts at: 788 788
Size: 4640
SfcI C'TryA_G
Cuts at: 33 326 1315 1683 2368 3361 3552 33
Size: 293 989 368 685 993 191 1121
Fragments arranged by size:
1121 993 989 685 368 293 191
SfoI GGC'GCC
Cuts at: 2072 2320 2072
Size: 248 4392
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 1662 1662
Size: 4640
SmlI C'TyrA_G
Cuts at: 553 850 1300 1795 2710 3202 3464 553
Size: 297 450 495 915 492 262 1729
Fragments arranged by size:
1729 915 495 492 450 297 262
SnaBI TAC'GTA
Cuts at: 1632 1632
Size: 4640
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
StyI C'CwwG_G
Cuts at: 1085 1774 2128 1085
Size: 689 354 3597
Fragments arranged by size:
3597 689 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 164 709 1522 2223 2356 2815 3024 164
Size: 545 813 701 133 459 209 1780
Fragments arranged by size:
1780 813 701 545 459 209 133
TatI w'GTAC_w
Cuts at: 351 1166 1526 4155 351
Size: 815 360 2629 836
Fragments arranged by size:
2629 836 815 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4157 4189 4157
Size: 32 4608
Tth111I GACn'n_nGTC
Cuts at: 2437 2437
Size: 4640
XbaI T'CTAG_A
Cuts at: 1671 1671
Size: 4640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 230 309 312 1002 1197 1212 1410 2135 230
Size: 79 3 690 195 15 198 725 2735
Fragments arranged by size:
2735 725 690 198 195 79 15 3
XmaI C'CCGG_G
Cuts at: 1660 1660
Size: 4640
XmnI GAAnn'nnTTC
Cuts at: 548 548
Size: 4640
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BsaXI BsiWI
BssHII BstZ17I Bsu36I ClaI EcoNI FseI FspAI HpaI
MfeI NcoI NdeI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI SspI StuI SwaI XhoI ZraI