Restriction map for
pF1KSDA1904
(Circular) (Six-base) MAPSORT of: pF1KSDA1904.seq Check: 9270 from: 1 to: 5597
pF1KSDA1904 5597 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1252 1252
Size: 5597
AatII G_ACGT'C
Cuts at: 1133 1133
Size: 5597
AccI GT'mk_AC
Cuts at: 2635 2635
Size: 5597
Acc65I G'GTAC_C
Cuts at: 2613 2613
Size: 5597
AclI AA'CG_TT
Cuts at: 5437 5437
Size: 5597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3422 3854 4601 4869 3422
Size: 432 747 268 4150
Fragments arranged by size:
4150 747 432 268
AfeI AGC'GCT
Cuts at: 2440 2440
Size: 5597
AflIII A'CryG_T
Cuts at: 224 2949 4053 5030 224
Size: 2725 1104 977 791
Fragments arranged by size:
2725 1104 977 791
AgeI A'CCGG_T
Cuts at: 3985 3985
Size: 5597
AleI CACnn'nnGTG
Cuts at: 1057 1057
Size: 5597
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 382 414 382
Size: 32 5565
AlwNI CAG_nnn'CTG
Cuts at: 447 1500 1879 1897 2583 2902 4469 447
Size: 1053 379 18 686 319 1567 1575
Fragments arranged by size:
1575 1567 1053 686 379 319 18
ApaI G_GGCC'C
Cuts at: 1911 1911
Size: 5597
ApaLI G'TGCA_C
Cuts at: 295 1483 4367 295
Size: 1188 2884 1525
Fragments arranged by size:
2884 1525 1188
ApoI r'AATT_y
Cuts at: 2601 4950 5206 2601
Size: 2349 256 2992
Fragments arranged by size:
2992 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5597
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5597
AvaI C'yCGr_G
Cuts at: 576 942 1675 1696 1840 1903 2401 2617 576
Size: 366 733 21 144 63 498 216 3556
Fragments arranged by size:
3556 733 498 366 216 144 63 21
BamHI G'GATC_C
Cuts at: 2622 2622
Size: 5597
BanI G'GyrC_C
Cuts at: 178 415 628 807 1287 1648 2163 2284 2613
Size: 237 213 179 480 361 515 121 329
Cuts at: 2613 3027 3275 3310 178
Size: 414 248 35 2465
Fragments arranged by size:
2465 515 480 414 361 329 248 237
213 179 121 35
BanII G_rGCy'C
Cuts at: 1714 1911 1954 2611 3641 1714
Size: 197 43 657 1030 3670
Fragments arranged by size:
3670 1030 657 197 43
BbeI G_GCGC'C
Cuts at: 1291 1652 3031 3279 1291
Size: 361 1379 248 3609
Fragments arranged by size:
3609 1379 361 248
BbsI GAAGACnn'nnnn_
Cuts at: 723 1438 723
Size: 715 4882
BbvCI CC'TCA_GC
Cuts at: 613 1890 613
Size: 1277 4320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 397 431 1335 1369 1614 1648 2266 2300 397
Size: 34 904 34 245 34 618 34 3694
Fragments arranged by size:
3694 904 618 245 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3495 4256 5597 3495
Size: 761 1341 3495
Fragments arranged by size:
3495 1341 761
BclI T'GATC_A
Cuts at: 3117 3117
Size: 5597
BglI GCCn_nnn'nGGC
Cuts at: 882 1343 1990 882
Size: 461 647 4489
Fragments arranged by size:
4489 647 461
BglII A'GATC_T
Cuts at: 395 1724 2429 3112 5149 395
Size: 1329 705 683 2037 843
Fragments arranged by size:
2037 1329 843 705 683
BlpI GC'TnA_GC
Cuts at: 469 1978 2709 469
Size: 1509 731 3357
Fragments arranged by size:
3357 1509 731
Bme1580I G_kGCm'C
Cuts at: 299 577 812 1487 1911 2069 3222 3315 4371
Size: 278 235 675 424 158 1153 93 1056
Cuts at: 4371 299
Size: 1525
Fragments arranged by size:
1525 1153 1056 675 424 278 235 158
93
BmgBI CAC'GTC
Cuts at: 1035 2064 1035
Size: 1029 4568
BmrI ACTGGGnnnn_n'
Cuts at: 2809 2890 3225 5369 2809
Size: 81 335 2144 3037
Fragments arranged by size:
3037 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3964 3964
Size: 5597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1462 1933 2236 2379 832
Size: 630 471 303 143 4050
Fragments arranged by size:
4050 630 471 303 143
Bpu10I CC'TnA_GC
Cuts at: 613 1890 2096 2296 613
Size: 1277 206 200 3914
Fragments arranged by size:
3914 1277 206 200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1774 2302 2773 3652 4144 4442 1126
Size: 648 528 471 879 492 298 2281
Fragments arranged by size:
2281 879 648 528 492 471 298
BsaI GGTCTCn'nnnn_
Cuts at: 259 2571 5301 259
Size: 2312 2730 555
Fragments arranged by size:
2730 2312 555
BsaAI yAC'GTr
Cuts at: 225 2589 2950 225
Size: 2364 361 2872
Fragments arranged by size:
2872 2364 361
BsaBI GATnn'nnATC
Cuts at: 6 1093 3111 5148 6
Size: 1087 2018 2037 455
Fragments arranged by size:
2037 2018 1087 455
BsaHI Gr'CG_yC
Cuts at: 553 917 1130 1288 1649 3028 3276 3978 5474
Size: 364 213 158 361 1379 248 702 1496
Cuts at: 5474 553
Size: 676
Fragments arranged by size:
1496 1379 702 676 364 361 248 213
158
BsaWI w'CCGG_w
Cuts at: 2786 3307 3985 4259 4406 4817 2786
Size: 521 678 274 147 411 3566
Fragments arranged by size:
3566 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 382 412 586 616 2461 2491 382
Size: 30 174 30 1845 30 3488
Fragments arranged by size:
3488 1845 174 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 1417 1878 2293 2382 2485 333
Size: 1084 461 415 89 103 3445
Fragments arranged by size:
3445 1084 461 415 103 89
BseYI C'CCAG_C
Cuts at: 453 1291 1359 1500 1921 2573 3022 3780 4357
Size: 838 68 141 421 652 449 758 577
Cuts at: 4357 4853 453
Size: 496 1197
Fragments arranged by size:
1197 838 758 652 577 496 449 421
141 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1123 1012
Size: 111 5486
BsiEI CG_ry'CG
Cuts at: 104 175 759 1994 2081 3139 3185 4393 104
Size: 71 584 1235 87 1058 46 1208 1308
Fragments arranged by size:
1308 1235 1208 1058 584 87 71 46
BsiHKAI G_wGCw'C
Cuts at: 299 338 1487 1954 2249 2422 2611 3389 3579
Size: 39 1149 467 295 173 189 778 190
Cuts at: 3579 4371 299
Size: 792 1525
Fragments arranged by size:
1525 1149 792 778 467 295 190 189
173 39
Bsp1286I G_dGCh'C
Cuts at: 299 338 577 812 1487 1714 1911 1954 2069
Size: 39 239 235 675 227 197 43 115
Cuts at: 2069 2249 2422 2611 3222 3315 3389 3579 3641
Size: 180 173 189 611 93 74 190 62
Cuts at: 3641 4371 299
Size: 730 1525
Fragments arranged by size:
1525 730 675 611 239 235 227 197
190 189 180 173 115 93 74 62
43 39
BspEI T'CCGG_A
Cuts at: 4817 4817
Size: 5597
BspHI T'CATG_A
Cuts at: 1133 1319 4773 1133
Size: 186 3454 1957
Fragments arranged by size:
3454 1957 186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 311 432 1252 2017 2647 2861 3163 3544 311
Size: 121 820 765 630 214 302 381 2364
Fragments arranged by size:
2364 820 765 630 381 302 214 121
BsrBI CCG'CTC
Cuts at: 50 1917 2333 3889 3943 5427 5592 50
Size: 1867 416 1556 54 1484 165 55
Fragments arranged by size:
1867 1556 1484 416 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1733 3509 1733
Size: 1776 3821
BsrFI r'CCGG_y
Cuts at: 170 673 790 981 1471 1651 2150 2166 2226
Size: 503 117 191 490 180 499 16 60
Cuts at: 2226 2249 2488 3595 3776 3985 170
Size: 23 239 1107 181 209 1782
Fragments arranged by size:
1782 1107 503 499 490 239 209 191
181 180 117 60 23 16
BsrGI T'GTAC_A
Cuts at: 1091 2477 1091
Size: 1386 4211
BssSI C'ACGA_G
Cuts at: 1099 3868 4226 1099
Size: 2769 358 2470
Fragments arranged by size:
2769 2470 358
BstAPI GCAn_nnn'nTGC
Cuts at: 303 447 1879 2009 2974 303
Size: 144 1432 130 965 2926
Fragments arranged by size:
2926 1432 965 144 130
BstBI TT'CG_AA
Cuts at: 110 3958 110
Size: 3848 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 657 1059 1584 1746 2091 657
Size: 402 525 162 345 4163
Fragments arranged by size:
4163 525 402 345 162
BstYI r'GATC_y
Cuts at: 1 395 962 1724 2429 2532 2622 3089 3112
Size: 394 567 762 705 103 90 467 23
Cuts at: 3112 3447 3693 4705 4791 4803 5149 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 762 705 567 467 449 394 346
335 246 103 90 86 23 12
BtgI C'CryG_G
Cuts at: 778 995 778
Size: 217 5380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3547 3728 3891 3547
Size: 181 163 5253
Fragments arranged by size:
5253 181 163
BtsI GCAGTG_nn'
Cuts at: 772 1836 2235 2840 772
Size: 1064 399 605 3529
Fragments arranged by size:
3529 1064 605 399
ClaI AT'CG_AT
Cuts at: 1781 1781
Size: 5597
DraI TTT'AAA
Cuts at: 2596 2596
Size: 5597
DrdI GACnn_nn'nnGTC
Cuts at: 3303 4161 3303
Size: 858 4739
EaeI y'GGCC_r
Cuts at: 172 233 671 1049 1704 1991 2078 2483 3182
Size: 61 438 378 655 287 87 405 699
Cuts at: 3182 3356 3747 3774 5119 172
Size: 174 391 27 1345 650
Fragments arranged by size:
1345 699 655 650 438 405 391 378
287 174 87 61 27
EagI C'GGCC_G
Cuts at: 172 1991 2078 3182 172
Size: 1819 87 1104 2587
Fragments arranged by size:
2587 1819 1104 87
EarI CTCTTCn'nnn_
Cuts at: 709 3620 3830 709
Size: 2911 210 2476
Fragments arranged by size:
2911 2476 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2559 3805 4115 4261 5407 2559
Size: 1246 310 146 1146 2749
Fragments arranged by size:
2749 1246 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1952 2609 1952
Size: 657 4940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1462 1933 2236 2379 3422 3854 4601 4869
Size: 630 471 303 143 1043 432 747 268
Cuts at: 4869 832
Size: 1560
Fragments arranged by size:
1560 1043 747 630 471 432 303 268
143
EcoNI CCTnn'n_nnAGG
Cuts at: 481 541 481
Size: 60 5537
EcoO109I rG'GnC_Cy
Cuts at: 444 1266 1836 1907 1908 2736 444
Size: 822 570 71 1 828 3305
Fragments arranged by size:
3305 828 822 570 71 1
EcoRI G'AATT_C
Cuts at: 2601 2601
Size: 5597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3616 3616
Size: 5597
FspI TGC'GCA
Cuts at: 1219 1384 1642 2503 3378 1219
Size: 165 258 861 875 3438
Fragments arranged by size:
3438 875 861 258 165
FspAI rTGC'GCAy
Cuts at: 1642 1642
Size: 5597
HaeII r_GCGC'y
Cuts at: 157 1291 1652 1974 1985 2045 2442 3031 3279
Size: 1134 361 322 11 60 397 589 248
Cuts at: 3279 4301 5280 157
Size: 1022 979 474
Fragments arranged by size:
1134 1022 979 589 474 397 361 322
248 60 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 925 957 1459 1491 1789 1821 1846
Size: 32 307 32 502 32 298 32 25
Cuts at: 1846 1878 2395 2427 3439 3469 3471 3501 586
Size: 32 517 32 1012 30 2 30 2682
Fragments arranged by size:
2682 1012 517 502 307 298 32 32
32 32 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 1422 1758 2636 1422
Size: 336 878 4383
Fragments arranged by size:
4383 878 336
KasI G'GCGC_C
Cuts at: 1287 1648 3027 3275 1287
Size: 361 1379 248 3609
Fragments arranged by size:
3609 1379 361 248
KpnI G_GTAC'C
Cuts at: 2617 2617
Size: 5597
MluI A'CGCG_T
Cuts at: 5030 5030
Size: 5597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 629 841 1151 1565 4268 4452 629
Size: 212 310 414 2703 184 1774
Fragments arranged by size:
2703 1774 414 310 212 184
MscI TGG'CCA
Cuts at: 235 3358 235
Size: 3123 2474
MslI CAynn'nnrTG
Cuts at: 544 1057 1099 3586 3713 544
Size: 513 42 2487 127 2428
Fragments arranged by size:
2487 2428 513 127 42
MspA1I CmG'CkG
Cuts at: 453 780 801 1876 1977 2145 2160 2177 2189
Size: 327 21 1075 101 168 15 17 12
Cuts at: 2189 2325 2708 3022 3382 4395 4640 453
Size: 136 383 314 360 1013 245 1410
Fragments arranged by size:
1410 1075 1013 383 360 327 314 245
168 136 101 21 17 15 12
NaeI GCC'GGC
Cuts at: 172 675 792 983 1473 1653 2168 2228 3778
Size: 503 117 191 490 180 515 60 1550
Cuts at: 3778 172
Size: 1991
Fragments arranged by size:
1991 1550 515 503 490 191 180 117
60
NarI GG'CG_CC
Cuts at: 1288 1649 3028 3276 1288
Size: 361 1379 248 3609
Fragments arranged by size:
3609 1379 361 248
NgoMIV G'CCGG_C
Cuts at: 170 673 790 981 1471 1651 2166 2226 3776
Size: 503 117 191 490 180 515 60 1550
Cuts at: 3776 170
Size: 1991
Fragments arranged by size:
1991 1550 515 503 490 191 180 117
60
NspI r_CATG'y
Cuts at: 492 1398 2650 3150 4057 492
Size: 906 1252 500 907 2032
Fragments arranged by size:
2032 1252 907 906 500
PciI A'CATG_T
Cuts at: 4053 4053
Size: 5597
PflMI CCAn_nnn'nTGG
Cuts at: 1500 2214 1500
Size: 714 4883
PfoI T'CCnGG_A
Cuts at: 1439 1688 1439
Size: 249 5348
PmeI GTTT'AAAC
Cuts at: 2596 2596
Size: 5597
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 382 414 4736 4768 382
Size: 32 4322 32 1211
Fragments arranged by size:
4322 1211 32 32
PpuMI rG'GwC_Cy
Cuts at: 444 1266 1836 444
Size: 822 570 4205
Fragments arranged by size:
4205 822 570
PspOMI G'GGCC_C
Cuts at: 1907 1907
Size: 5597
PstI C_TGCA'G
Cuts at: 244 443 452 515 767 1722 1875 2014 2242
Size: 199 9 63 252 955 153 139 228
Cuts at: 2242 2644 3329 244
Size: 402 685 2512
Fragments arranged by size:
2512 955 685 402 252 228 199 153
139 63 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5597
PvuII CAG'CTG
Cuts at: 453 1876 1977 3022 3382 453
Size: 1423 101 1045 360 2668
Fragments arranged by size:
2668 1423 1045 360 101
RsrII CG'GwC_CG
Cuts at: 3792 3792
Size: 5597
SacI G_AGCT'C
Cuts at: 1954 2611 1954
Size: 657 4940
SacII CC_GC'GG
Cuts at: 781 781
Size: 5597
SalI G'TCGA_C
Cuts at: 2634 2634
Size: 5597
SanDI GG'GwC_CC
Cuts at: 1266 1836 1266
Size: 570 5027
SapI GCTCTTCn'nnn_
Cuts at: 3620 3830 3620
Size: 210 5387
SbfI CC_TGCA'GG
Cuts at: 443 2644 443
Size: 2201 3396
ScaI AGT'ACT
Cuts at: 2407 5114 2407
Size: 2707 2890
SfcI C'TryA_G
Cuts at: 33 240 439 448 511 763 1080 1718 1871
Size: 207 199 9 63 252 317 638 153
Cuts at: 1871 1966 2010 2238 2640 3325 4318 4509 33
Size: 95 44 228 402 685 993 191 1121
Fragments arranged by size:
1121 993 685 638 402 317 252 228
207 199 191 153 95 63 44 9
SfoI GGC'GCC
Cuts at: 1289 1650 3029 3277 1289
Size: 361 1379 248 3609
Fragments arranged by size:
3609 1379 361 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5597
SgrAI Cr'CCGG_yG
Cuts at: 1651 1651
Size: 5597
SmaI CCC'GGG
Cuts at: 1905 2619 1905
Size: 714 4883
SmlI C'TyrA_G
Cuts at: 1141 1696 1789 2317 2752 3667 4159 4421 1141
Size: 555 93 528 435 915 492 262 2317
Fragments arranged by size:
2317 915 555 528 492 435 262 93
SnaBI TAC'GTA
Cuts at: 2589 2589
Size: 5597
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5597
SphI G_CATG'C
Cuts at: 492 1398 2650 3150 492
Size: 906 1252 500 2939
Fragments arranged by size:
2939 1252 906 500
StyI C'CwwG_G
Cuts at: 1577 1607 1739 2045 2522 2731 3085 1577
Size: 30 132 306 477 209 354 4089
Fragments arranged by size:
4089 477 354 306 209 132 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1324 1620 1976 3180 3313 3772 3981 1324
Size: 296 356 1204 133 459 209 2940
Fragments arranged by size:
2940 1204 459 356 296 209 133
TatI w'GTAC_w
Cuts at: 1091 1106 1193 1370 2111 2270 2405 2477 5112
Size: 15 87 177 741 159 135 72 2635
Cuts at: 5112 1091
Size: 1576
Fragments arranged by size:
2635 1576 741 177 159 135 87 72
15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5114 5146 5114
Size: 32 5565
Tth111I GACn'n_nGTC
Cuts at: 1270 1753 3394 1270
Size: 483 1641 3473
Fragments arranged by size:
3473 1641 483
XbaI T'CTAG_A
Cuts at: 2628 2628
Size: 5597
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 3092 132
Size: 2960 2637
XhoI C'TCGA_G
Cuts at: 1696 1696
Size: 5597
XmaI C'CCGG_G
Cuts at: 1903 2617 1903
Size: 714 4883
ZraI GAC'GTC
Cuts at: 1131 1131
Size: 5597
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I CspCI
DraIII EcoRV FseI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspXI PsrI SexAI SfiI SrfI SspI StuI
SwaI XmnI