Restriction map for
pF1KSDA1911
(Circular) (Six-base) MAPSORT of: pF1KSDA1911.seq Check: 683 from: 1 to: 5657
pF1KSDA1911 5657 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1254 2002 2695 1254
Size: 748 693 4216
Fragments arranged by size:
4216 748 693
Acc65I G'GTAC_C
Cuts at: 2673 2673
Size: 5657
AclI AA'CG_TT
Cuts at: 5497 5497
Size: 5657
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1526 2162 3482 3914 4661 4929 869
Size: 657 636 1320 432 747 268 1597
Fragments arranged by size:
1597 1320 747 657 636 432 268
AflIII A'CryG_T
Cuts at: 645 3009 4113 5090 645
Size: 2364 1104 977 1212
Fragments arranged by size:
2364 1212 1104 977
AgeI A'CCGG_T
Cuts at: 4045 4045
Size: 5657
AleI CACnn'nnGTG
Cuts at: 2226 2226
Size: 5657
AlwNI CAG_nnn'CTG
Cuts at: 979 2037 2416 2962 4529 979
Size: 1058 379 546 1567 2107
Fragments arranged by size:
2107 1567 1058 546 379
ApaLI G'TGCA_C
Cuts at: 4427 4427
Size: 5657
ApoI r'AATT_y
Cuts at: 156 192 206 545 720 1318 1656 1762 2096
Size: 36 14 339 175 598 338 106 334
Cuts at: 2096 2661 5010 5266 156
Size: 565 2349 256 547
Fragments arranged by size:
2349 598 565 547 339 338 334 256
175 106 36 14
AseI AT'TA_AT
Cuts at: 20 374 707 1499 1520 1541 2627 20
Size: 354 333 792 21 21 1086 3050
Fragments arranged by size:
3050 1086 792 354 333 21 21
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5657
AvaI C'yCGr_G
Cuts at: 2677 2677
Size: 5657
BamHI G'GATC_C
Cuts at: 2682 2682
Size: 5657
BanI G'GyrC_C
Cuts at: 2673 3087 3335 3370 2673
Size: 414 248 35 4960
Fragments arranged by size:
4960 414 248 35
BanII G_rGCy'C
Cuts at: 1563 2671 3701 1563
Size: 1108 1030 3519
Fragments arranged by size:
3519 1108 1030
BbeI G_GCGC'C
Cuts at: 3091 3339 3091
Size: 248 5409
BbsI GAAGACnn'nnnn_
Cuts at: 820 2150 820
Size: 1330 4327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 825 859 1195 1229 825
Size: 34 336 34 5253
Fragments arranged by size:
5253 336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3555 4316 5657 3555
Size: 761 1341 3555
Fragments arranged by size:
3555 1341 761
BclI T'GATC_A
Cuts at: 3177 3177
Size: 5657
BfrBI ATG'CAT
Cuts at: 1413 1413
Size: 5657
BglII A'GATC_T
Cuts at: 3172 5209 3172
Size: 2037 3620
BlpI GC'TnA_GC
Cuts at: 1275 2769 1275
Size: 1494 4163
Bme1580I G_kGCm'C
Cuts at: 3282 3375 4431 3282
Size: 93 1056 4508
Fragments arranged by size:
4508 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2869 2950 3285 5429 2869
Size: 81 335 2144 3097
Fragments arranged by size:
3097 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 177 4024 177
Size: 3847 1810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1021
Size: 5657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1337 2833 3712 4204 4502 1337
Size: 1496 879 492 298 2492
Fragments arranged by size:
2492 1496 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1147 5361 1147
Size: 4214 1443
BsaAI yAC'GTr
Cuts at: 2645 2649 3010 2645
Size: 4 361 5292
Fragments arranged by size:
5292 361 4
BsaBI GATnn'nnATC
Cuts at: 6 695 3171 5208 6
Size: 689 2476 2037 455
Fragments arranged by size:
2476 2037 689 455
BsaHI Gr'CG_yC
Cuts at: 3088 3336 4038 5534 3088
Size: 248 702 1496 3211
Fragments arranged by size:
3211 1496 702 248
BsaWI w'CCGG_w
Cuts at: 482 2846 3367 4045 4319 4466 4877 482
Size: 2364 521 678 274 147 411 1262
Fragments arranged by size:
2364 1262 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 3082 3840 4417 4913 3082
Size: 758 577 496 3826
Fragments arranged by size:
3826 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 1351
Size: 5657
BsiEI CG_ry'CG
Cuts at: 104 3199 3245 4453 104
Size: 3095 46 1208 1308
Fragments arranged by size:
3095 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2671 3449 3639 4431 2671
Size: 778 190 792 3897
Fragments arranged by size:
3897 792 778 190
BsiWI C'GTAC_G
Cuts at: 2645 2645
Size: 5657
BsmI GAATG_Cn'
Cuts at: 2000 2497 2000
Size: 497 5160
BsmBI CGTCTCn'nnnn_
Cuts at: 821 821
Size: 5657
Bsp1286I G_dGCh'C
Cuts at: 1563 2671 3282 3375 3449 3639 3701 4431 1563
Size: 1108 611 93 74 190 62 730 2789
Fragments arranged by size:
2789 1108 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4877 4877
Size: 5657
BspHI T'CATG_A
Cuts at: 4833 4833
Size: 5657
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2707 2921 3223 3604 2707
Size: 214 302 381 4760
Fragments arranged by size:
4760 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3949 4003 5487 5652 50
Size: 3899 54 1484 165 55
Fragments arranged by size:
3899 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1213 3569 1213
Size: 2356 3301
BsrFI r'CCGG_y
Cuts at: 3655 3836 4045 3655
Size: 181 209 5267
Fragments arranged by size:
5267 209 181
BssSI C'ACGA_G
Cuts at: 3928 4286 3928
Size: 358 5299
BstAPI GCAn_nnn'nTGC
Cuts at: 3034 3034
Size: 5657
BstBI TT'CG_AA
Cuts at: 110 4018 110
Size: 3908 1749
BstYI r'GATC_y
Cuts at: 1 793 2553 2682 3149 3172 3507 3753 4765
Size: 792 1760 129 467 23 335 246 1012
Cuts at: 4765 4851 4863 5209 1
Size: 86 12 346 449
Fragments arranged by size:
1760 1012 792 467 449 346 335 246
129 86 23 12
BstZ17I GTA'TAC
Cuts at: 1255 2003 1255
Size: 748 4909
Bsu36I CC'TnA_GG
Cuts at: 2497 2497
Size: 5657
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3607 3788 3951 3607
Size: 181 163 5313
Fragments arranged by size:
5313 181 163
BtsI GCAGTG_nn'
Cuts at: 607 1827 2900 607
Size: 1220 1073 3364
Fragments arranged by size:
3364 1220 1073
DraI TTT'AAA
Cuts at: 2656 2656
Size: 5657
DrdI GACnn_nn'nnGTC
Cuts at: 3363 4221 3363
Size: 858 4799
EaeI y'GGCC_r
Cuts at: 1580 3242 3416 3807 3834 5179 1580
Size: 1662 174 391 27 1345 2058
Fragments arranged by size:
2058 1662 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3242 3242
Size: 5657
EarI CTCTTCn'nnn_
Cuts at: 646 819 1145 2260 2324 3680 3890 646
Size: 173 326 1115 64 1356 210 2413
Fragments arranged by size:
2413 1356 1115 326 210 173 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3865 4175 4321 5467 3865
Size: 310 146 1146 4055
Fragments arranged by size:
4055 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2669 2669
Size: 5657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1021 1526 2162 3482 3914 4661 4929 869
Size: 152 505 636 1320 432 747 268 1597
Fragments arranged by size:
1597 1320 747 636 505 432 268 152
EcoNI CCTnn'n_nnAGG
Cuts at: 1367 2164 1367
Size: 797 4860
EcoO109I rG'GnC_Cy
Cuts at: 2796 2796
Size: 5657
EcoRI G'AATT_C
Cuts at: 156 192 2096 2661 156
Size: 36 1904 565 3152
Fragments arranged by size:
3152 1904 565 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 3676 2353
Size: 1323 4334
FspI TGC'GCA
Cuts at: 3438 3438
Size: 5657
HaeII r_GCGC'y
Cuts at: 3091 3339 4361 5340 3091
Size: 248 1022 979 3408
Fragments arranged by size:
3408 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1630 1662 3499 3529 3531 3561 1630
Size: 32 1837 30 2 30 3726
Fragments arranged by size:
3726 1837 32 30 30 2
HincII GTy'rAC
Cuts at: 2172 2193 2696 2172
Size: 21 503 5133
Fragments arranged by size:
5133 503 21
KasI G'GCGC_C
Cuts at: 3087 3335 3087
Size: 248 5409
KpnI G_GTAC'C
Cuts at: 2677 2677
Size: 5657
MluI A'CGCG_T
Cuts at: 5090 5090
Size: 5657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1325 2443 4328 4512 1316
Size: 9 1118 1885 184 2461
Fragments arranged by size:
2461 1885 1118 184 9
MscI TGG'CCA
Cuts at: 1582 3418 1582
Size: 1836 3821
MslI CAynn'nnrTG
Cuts at: 2226 3646 3773 2226
Size: 1420 127 4110
Fragments arranged by size:
4110 1420 127
MspA1I CmG'CkG
Cuts at: 2768 3082 3442 4455 4700 2768
Size: 314 360 1013 245 3725
Fragments arranged by size:
3725 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3838 3838
Size: 5657
NarI GG'CG_CC
Cuts at: 3088 3336 3088
Size: 248 5409
NgoMIV G'CCGG_C
Cuts at: 3836 3836
Size: 5657
NsiI A_TGCA'T
Cuts at: 1415 1415
Size: 5657
NspI r_CATG'y
Cuts at: 649 2710 3210 4117 649
Size: 2061 500 907 2189
Fragments arranged by size:
2189 2061 907 500
PciI A'CATG_T
Cuts at: 645 4113 645
Size: 3468 2189
PflMI CCAn_nnn'nTGG
Cuts at: 1671 1671
Size: 5657
PmeI GTTT'AAAC
Cuts at: 2656 2656
Size: 5657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4796 4828 4796
Size: 32 5625
PsiI TTA'TAA
Cuts at: 2059 2059
Size: 5657
PstI C_TGCA'G
Cuts at: 2704 3389 2704
Size: 685 4972
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5657
PvuII CAG'CTG
Cuts at: 3082 3442 3082
Size: 360 5297
RsrII CG'GwC_CG
Cuts at: 3852 3852
Size: 5657
SacI G_AGCT'C
Cuts at: 2671 2671
Size: 5657
SalI G'TCGA_C
Cuts at: 2694 2694
Size: 5657
SapI GCTCTTCn'nnn_
Cuts at: 3680 3890 3680
Size: 210 5447
SbfI CC_TGCA'GG
Cuts at: 2704 2704
Size: 5657
ScaI AGT'ACT
Cuts at: 612 662 2590 5174 612
Size: 50 1928 2584 1095
Fragments arranged by size:
2584 1928 1095 50
SfcI C'TryA_G
Cuts at: 33 2316 2700 3385 4378 4569 33
Size: 2283 384 685 993 191 1121
Fragments arranged by size:
2283 1121 993 685 384 191
SfoI GGC'GCC
Cuts at: 3089 3337 3089
Size: 248 5409
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5657
SmaI CCC'GGG
Cuts at: 2679 2679
Size: 5657
SmlI C'TyrA_G
Cuts at: 1352 2812 3727 4219 4481 1352
Size: 1460 915 492 262 2528
Fragments arranged by size:
2528 1460 915 492 262
SnaBI TAC'GTA
Cuts at: 2649 2649
Size: 5657
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5657
SphI G_CATG'C
Cuts at: 2710 3210 2710
Size: 500 5157
StyI C'CwwG_G
Cuts at: 2791 3145 2791
Size: 354 5303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3240 3373 3832 4041 3240
Size: 133 459 209 4856
Fragments arranged by size:
4856 459 209 133
TatI w'GTAC_w
Cuts at: 325 447 610 660 1432 2588 5172 325
Size: 122 163 50 772 1156 2584 810
Fragments arranged by size:
2584 1156 810 772 163 122 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5174 5206 5174
Size: 32 5625
Tth111I GACn'n_nGTC
Cuts at: 3454 3454
Size: 5657
XbaI T'CTAG_A
Cuts at: 2688 2688
Size: 5657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3152 3152
Size: 5657
XmaI C'CCGG_G
Cuts at: 2677 2677
Size: 5657
XmnI GAAnn'nnTTC
Cuts at: 1848 2096 1848
Size: 248 5409
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsrGI BssHII BstEII BstXI BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
ZraI