Restriction map for
pF1KSDA1912
(Circular) (Six-base) MAPSORT of: pF1KSDA1912.seq Check: 2422 from: 1 to: 4796
pF1KSDA1912 4796 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 842 842
Size: 4796
AccI GT'mk_AC
Cuts at: 1349 1834 1349
Size: 485 4311
Acc65I G'GTAC_C
Cuts at: 1812 1812
Size: 4796
AclI AA'CG_TT
Cuts at: 4636 4636
Size: 4796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1626 2621 3053 3800 4068 1126
Size: 500 995 432 747 268 1854
Fragments arranged by size:
1854 995 747 500 432 268
AflII C'TTAA_G
Cuts at: 1693 1693
Size: 4796
AflIII A'CryG_T
Cuts at: 2148 3252 4229 2148
Size: 1104 977 2715
Fragments arranged by size:
2715 1104 977
AgeI A'CCGG_T
Cuts at: 1085 3184 1085
Size: 2099 2697
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1602 1634 1602
Size: 32 4764
AlwNI CAG_nnn'CTG
Cuts at: 438 510 567 677 917 1603 2101 3668 438
Size: 72 57 110 240 686 498 1567 1566
Fragments arranged by size:
1567 1566 686 498 240 110 72 57
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 1800 4149 4405 1800
Size: 2349 256 2191
Fragments arranged by size:
2349 2191 256
AscI GG'CGCG_CC
Cuts at: 297 297
Size: 4796
AseI AT'TA_AT
Cuts at: 20 20
Size: 4796
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 494 892 1575 1816 494
Size: 398 683 241 3474
Fragments arranged by size:
3474 683 398 241
BamHI G'GATC_C
Cuts at: 1821 1821
Size: 4796
BanI G'GyrC_C
Cuts at: 1007 1192 1297 1541 1812 2226 2474 2509 1007
Size: 185 105 244 271 414 248 35 3294
Fragments arranged by size:
3294 414 271 248 244 185 105 35
BanII G_rGCy'C
Cuts at: 620 629 1066 1132 1234 1515 1810 2840 620
Size: 9 437 66 102 281 295 1030 2576
Fragments arranged by size:
2576 1030 437 295 281 102 66 9
BbeI G_GCGC'C
Cuts at: 2230 2478 2230
Size: 248 4548
BbsI GAAGACnn'nnnn_
Cuts at: 431 431
Size: 4796
BbvCI CC'TCA_GC
Cuts at: 183 183
Size: 4796
BciVI GTATCCnnnnn_n'
Cuts at: 2694 3455 4796 2694
Size: 761 1341 2694
Fragments arranged by size:
2694 1341 761
BclI T'GATC_A
Cuts at: 1236 2316 1236
Size: 1080 3716
BglII A'GATC_T
Cuts at: 2311 4348 2311
Size: 2037 2759
BlpI GC'TnA_GC
Cuts at: 1908 1908
Size: 4796
Bme1580I G_kGCm'C
Cuts at: 1195 1300 2421 2514 3570 1195
Size: 105 1121 93 1056 2421
Fragments arranged by size:
2421 1121 1056 105 93
BmrI ACTGGGnnnn_n'
Cuts at: 520 2008 2089 2424 4568 520
Size: 1488 81 335 2144 748
Fragments arranged by size:
2144 1488 748 335 81
BmtI G_CTAG'C
Cuts at: 1640 1640
Size: 4796
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 509 3163 509
Size: 2654 2142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 598 750 1414 1435 403
Size: 195 152 664 21 3764
Fragments arranged by size:
3764 664 195 152 21
Bpu10I CC'TnA_GC
Cuts at: 183 183
Size: 4796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 604 1972 2851 3343 3641 382
Size: 222 1368 879 492 298 1537
Fragments arranged by size:
1537 1368 879 492 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 4500 4500
Size: 4796
BsaAI yAC'GTr
Cuts at: 735 1050 1788 2149 735
Size: 315 738 361 3382
Fragments arranged by size:
3382 738 361 315
BsaBI GATnn'nnATC
Cuts at: 6 2310 4347 6
Size: 2304 2037 455
Fragments arranged by size:
2304 2037 455
BsaHI Gr'CG_yC
Cuts at: 2227 2475 3177 4673 2227
Size: 248 702 1496 2350
Fragments arranged by size:
2350 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1340 1985 2506 3184 3458 3605 4016 1085
Size: 255 645 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 645 521 411 274 255 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 1312 1342 1602 1632 217
Size: 30 1065 30 260 30 3381
Fragments arranged by size:
3381 1065 260 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 289 661 1265 265
Size: 24 372 604 3796
Fragments arranged by size:
3796 604 372 24
BseYI C'CCAG_C
Cuts at: 1499 1664 2221 2979 3556 4052 1499
Size: 165 557 758 577 496 2243
Fragments arranged by size:
2243 758 577 557 496 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 677 358
Size: 319 4477
BsiEI CG_ry'CG
Cuts at: 104 140 194 2338 2384 3592 104
Size: 36 54 2144 46 1208 1308
Fragments arranged by size:
2144 1308 1208 54 46 36
BsiHKAI G_wGCw'C
Cuts at: 620 629 833 869 968 1208 1810 2588 2778
Size: 9 204 36 99 240 602 778 190
Cuts at: 2778 3570 620
Size: 792 1846
Fragments arranged by size:
1846 792 778 602 240 204 190 99
36 9
Bsp1286I G_dGCh'C
Cuts at: 620 629 833 869 968 1066 1132 1195 1208
Size: 9 204 36 99 98 66 63 13
Cuts at: 1208 1234 1300 1515 1810 2421 2514 2588 2778
Size: 26 66 215 295 611 93 74 190
Cuts at: 2778 2840 3570 620
Size: 62 730 1846
Fragments arranged by size:
1846 730 611 295 215 204 190 99
98 93 74 66 66 63 62 36
26 13 9
BspEI T'CCGG_A
Cuts at: 4016 4016
Size: 4796
BspHI T'CATG_A
Cuts at: 3972 3972
Size: 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 566 842 1717 1846 2060 2362 2743 566
Size: 276 875 129 214 302 381 2619
Fragments arranged by size:
2619 875 381 302 276 214 129
BsrBI CCG'CTC
Cuts at: 50 3088 3142 4626 4791 50
Size: 3038 54 1484 165 55
Fragments arranged by size:
3038 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2708 2708
Size: 4796
BsrFI r'CCGG_y
Cuts at: 193 219 470 1085 2794 2975 3184 193
Size: 26 251 615 1709 181 209 1805
Fragments arranged by size:
1805 1709 615 251 209 181 26
BssHII G'CGCG_C
Cuts at: 297 297
Size: 4796
BssSI C'ACGA_G
Cuts at: 3067 3425 3067
Size: 358 4438
BstAPI GCAn_nnn'nTGC
Cuts at: 677 2173 677
Size: 1496 3300
BstBI TT'CG_AA
Cuts at: 110 3157 110
Size: 3047 1749
BstEII G'GTnAC_C
Cuts at: 1328 1328
Size: 4796
BstYI r'GATC_y
Cuts at: 1 386 1821 2288 2311 2646 2892 3904 3990
Size: 385 1435 467 23 335 246 1012 86
Cuts at: 3990 4002 4348 1
Size: 12 346 449
Fragments arranged by size:
1435 1012 467 449 385 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 203 390 203
Size: 187 4609
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 337 2746 2927 3090 337
Size: 2409 181 163 2043
Fragments arranged by size:
2409 2043 181 163
BtsI GCAGTG_nn'
Cuts at: 268 521 822 1263 1534 2039 268
Size: 253 301 441 271 505 3025
Fragments arranged by size:
3025 505 441 301 271 253
DraI TTT'AAA
Cuts at: 1795 1795
Size: 4796
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3360 2502
Size: 858 3938
EaeI y'GGCC_r
Cuts at: 137 191 2381 2555 2946 2973 4318 137
Size: 54 2190 174 391 27 1345 615
Fragments arranged by size:
2190 1345 615 391 174 54 27
EagI C'GGCC_G
Cuts at: 137 191 2381 137
Size: 54 2190 2552
Fragments arranged by size:
2552 2190 54
EarI CTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 184 322 1295 3004 3314 3460 4606 184
Size: 138 973 1709 310 146 1146 374
Fragments arranged by size:
1709 1146 973 374 310 146 138
EcoICRI GAG'CTC
Cuts at: 618 627 1808 618
Size: 9 1181 3606
Fragments arranged by size:
3606 1181 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 598 750 1126 1414 1435 1626 2621 3053
Size: 195 152 376 288 21 191 995 432
Cuts at: 3053 3800 4068 403
Size: 747 268 1131
Fragments arranged by size:
1131 995 747 432 376 288 268 195
191 152 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1383 1383
Size: 4796
EcoO109I rG'GnC_Cy
Cuts at: 228 444 952 1935 228
Size: 216 508 983 3089
Fragments arranged by size:
3089 983 508 216
EcoRI G'AATT_C
Cuts at: 1800 1800
Size: 4796
EcoRV GAT'ATC
Cuts at: 405 405
Size: 4796
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4796
FspI TGC'GCA
Cuts at: 666 2577 666
Size: 1911 2885
HaeII r_GCGC'y
Cuts at: 887 2230 2478 3500 4479 887
Size: 1343 248 1022 979 1204
Fragments arranged by size:
1343 1204 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 498 530 1221 1253 1312 1344 2638
Size: 32 249 32 691 32 59 32 1294
Cuts at: 2638 2668 2670 2700 217
Size: 30 2 30 2313
Fragments arranged by size:
2313 1294 691 249 59 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1835 1835
Size: 4796
KasI G'GCGC_C
Cuts at: 2226 2474 2226
Size: 248 4548
KpnI G_GTAC'C
Cuts at: 1816 1816
Size: 4796
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 224 1492 3467 3651 224
Size: 1268 1975 184 1369
Fragments arranged by size:
1975 1369 1268 184
MscI TGG'CCA
Cuts at: 2557 2557
Size: 4796
MslI CAynn'nnrTG
Cuts at: 1343 2785 2912 1343
Size: 1442 127 3227
Fragments arranged by size:
3227 1442 127
MspA1I CmG'CkG
Cuts at: 205 372 392 677 1277 1392 1413 1907 2221
Size: 167 20 285 600 115 21 494 314
Cuts at: 2221 2581 3594 3839 205
Size: 360 1013 245 1162
Fragments arranged by size:
1162 1013 600 494 360 314 285 245
167 115 21 20
NaeI GCC'GGC
Cuts at: 195 2977 195
Size: 2782 2014
NarI GG'CG_CC
Cuts at: 2227 2475 2227
Size: 248 4548
NgoMIV G'CCGG_C
Cuts at: 193 2975 193
Size: 2782 2014
NheI G'CTAG_C
Cuts at: 1636 1636
Size: 4796
NspI r_CATG'y
Cuts at: 1434 1654 1849 2349 3256 1434
Size: 220 195 500 907 2974
Fragments arranged by size:
2974 907 500 220 195
PciI A'CATG_T
Cuts at: 3252 3252
Size: 4796
PfoI T'CCnGG_A
Cuts at: 419 458 1737 419
Size: 39 1279 3478
Fragments arranged by size:
3478 1279 39
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PmlI CAC'GTG
Cuts at: 1050 1050
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1602 1634 3935 3967 1602
Size: 32 2301 32 2431
Fragments arranged by size:
2431 2301 32 32
PpuMI rG'GwC_Cy
Cuts at: 228 444 952 228
Size: 216 508 4072
Fragments arranged by size:
4072 508 216
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1667 1699 1667
Size: 32 4764
PstI C_TGCA'G
Cuts at: 263 371 563 839 1635 1728 1843 2528 263
Size: 108 192 276 796 93 115 685 2531
Fragments arranged by size:
2531 796 685 276 192 115 108 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 372 1392 1413 2221 2581 372
Size: 1020 21 808 360 2587
Fragments arranged by size:
2587 1020 808 360 21
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 620 629 1810 620
Size: 9 1181 3606
Fragments arranged by size:
3606 1181 9
SacII CC_GC'GG
Cuts at: 206 393 206
Size: 187 4609
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
SbfI CC_TGCA'GG
Cuts at: 1843 1843
Size: 4796
ScaI AGT'ACT
Cuts at: 4313 4313
Size: 4796
SfcI C'TryA_G
Cuts at: 33 259 367 559 835 1350 1631 1724 1839
Size: 226 108 192 276 515 281 93 115
Cuts at: 1839 2524 3517 3708 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 515 281 276 226 192
191 115 108 93
SfoI GGC'GCC
Cuts at: 2228 2476 2228
Size: 248 4548
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SgrAI Cr'CCGG_yG
Cuts at: 219 219
Size: 4796
SmaI CCC'GGG
Cuts at: 1577 1818 1577
Size: 241 4555
SmlI C'TyrA_G
Cuts at: 397 619 1693 1951 2866 3358 3620 397
Size: 222 1074 258 915 492 262 1573
Fragments arranged by size:
1573 1074 915 492 262 258 222
SnaBI TAC'GTA
Cuts at: 1788 1788
Size: 4796
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1434 1849 2349 1434
Size: 415 500 3881
Fragments arranged by size:
3881 500 415
StyI C'CwwG_G
Cuts at: 1930 2284 1930
Size: 354 4442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 736 1638 2379 2512 2971 3180 736
Size: 902 741 133 459 209 2352
Fragments arranged by size:
2352 902 741 459 209 133
TatI w'GTAC_w
Cuts at: 1769 4311 1769
Size: 2542 2254
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4313 4345 4313
Size: 32 4764
Tth111I GACn'n_nGTC
Cuts at: 512 2593 512
Size: 2081 2715
XbaI T'CTAG_A
Cuts at: 1827 1827
Size: 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 814 2291 814
Size: 1477 3319
XmaI C'CCGG_G
Cuts at: 1575 1816 1575
Size: 241 4555
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PciI PfoI PmeI PmlI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI ApaI AvrII BaeI
BcgI BfrBI BglI BmgBI BsiWI BsmI BsmBI BsrGI
BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NcoI NdeI NotI NruI NsiI
PacI PasI PflMI PshAI PsiI PspOMI PspXI SanDI
SexAI SfiI SrfI SspI StuI SwaI XhoI XmnI
ZraI