Restriction map for
pF1KSDA1916
(Circular) (Six-base) MAPSORT of: pF1KSDA1916.seq Check: 3230 from: 1 to: 4772
pF1KSDA1916 4772 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 201 201
Size: 4772
AccI GT'mk_AC
Cuts at: 1810 1810
Size: 4772
Acc65I G'GTAC_C
Cuts at: 1788 1788
Size: 4772
AclI AA'CG_TT
Cuts at: 4612 4612
Size: 4772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 2597 3029 3776 4044 567
Size: 2030 432 747 268 1295
Fragments arranged by size:
2030 1295 747 432 268
AfeI AGC'GCT
Cuts at: 155 155
Size: 4772
AflII C'TTAA_G
Cuts at: 1752 1752
Size: 4772
AflIII A'CryG_T
Cuts at: 2124 3228 4205 2124
Size: 1104 977 2691
Fragments arranged by size:
2691 1104 977
AgeI A'CCGG_T
Cuts at: 3160 3160
Size: 4772
AlwNI CAG_nnn'CTG
Cuts at: 1224 2077 3644 1224
Size: 853 1567 2352
Fragments arranged by size:
2352 1567 853
ApaLI G'TGCA_C
Cuts at: 3542 3542
Size: 4772
ApoI r'AATT_y
Cuts at: 402 628 681 918 1057 1066 1776 4125 4381
Size: 226 53 237 139 9 710 2349 256
Cuts at: 4381 402
Size: 793
Fragments arranged by size:
2349 793 710 256 237 226 139 53
9
AseI AT'TA_AT
Cuts at: 20 20
Size: 4772
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
AvaI C'yCGr_G
Cuts at: 157 1454 1508 1792 157
Size: 1297 54 284 3137
Fragments arranged by size:
3137 1297 284 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 706 739 706
Size: 33 4739
BamHI G'GATC_C
Cuts at: 1797 1797
Size: 4772
BanI G'GyrC_C
Cuts at: 181 288 1650 1788 2202 2450 2485 181
Size: 107 1362 138 414 248 35 2468
Fragments arranged by size:
2468 1362 414 248 138 107 35
BanII G_rGCy'C
Cuts at: 280 1786 2816 280
Size: 1506 1030 2236
Fragments arranged by size:
2236 1506 1030
BbeI G_GCGC'C
Cuts at: 185 1654 2206 2454 185
Size: 1469 552 248 2503
Fragments arranged by size:
2503 1469 552 248
BbsI GAAGACnn'nnnn_
Cuts at: 573 573
Size: 4772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1636 1670 1636
Size: 34 4738
BciVI GTATCCnnnnn_n'
Cuts at: 819 2670 3431 4772 819
Size: 1851 761 1341 819
Fragments arranged by size:
1851 1341 819 761
BclI T'GATC_A
Cuts at: 984 2292 984
Size: 1308 3464
BglII A'GATC_T
Cuts at: 2287 4324 2287
Size: 2037 2735
BlpI GC'TnA_GC
Cuts at: 1884 1884
Size: 4772
Bme1580I G_kGCm'C
Cuts at: 293 2397 2490 3546 293
Size: 2104 93 1056 1519
Fragments arranged by size:
2104 1519 1056 93
BmgBI CAC'GTC
Cuts at: 747 747
Size: 4772
BmrI ACTGGGnnnn_n'
Cuts at: 1984 2065 2400 4544 1984
Size: 81 335 2144 2212
Fragments arranged by size:
2212 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3139 3139
Size: 4772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1306 916
Size: 390 4382
Bpu10I CC'TnA_GC
Cuts at: 322 322
Size: 4772
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1948 2827 3319 3617 1948
Size: 879 492 298 3103
Fragments arranged by size:
3103 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1089 4476 1089
Size: 3387 1385
BsaAI yAC'GTr
Cuts at: 855 1764 2125 855
Size: 909 361 3502
Fragments arranged by size:
3502 909 361
BsaBI GATnn'nnATC
Cuts at: 6 2286 4323 6
Size: 2280 2037 455
Fragments arranged by size:
2280 2037 455
BsaHI Gr'CG_yC
Cuts at: 182 1651 2203 2451 3153 4649 182
Size: 1469 552 248 702 1496 305
Fragments arranged by size:
1496 1469 702 552 305 248
BsaWI w'CCGG_w
Cuts at: 923 1961 2482 3160 3434 3581 3992 923
Size: 1038 521 678 274 147 411 1703
Fragments arranged by size:
1703 1038 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 301
Size: 30 4742
BseYI C'CCAG_C
Cuts at: 177 871 1007 2197 2955 3532 4028 177
Size: 694 136 1190 758 577 496 921
Fragments arranged by size:
1190 921 758 694 577 496 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 1666 1210
Size: 456 4316
BsiEI CG_ry'CG
Cuts at: 104 2314 2360 3568 104
Size: 2210 46 1208 1308
Fragments arranged by size:
2210 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1786 2564 2754 3546 1786
Size: 778 190 792 3012
Fragments arranged by size:
3012 792 778 190
BsmI GAATG_Cn'
Cuts at: 772 1412 772
Size: 640 4132
BsmBI CGTCTCn'nnnn_
Cuts at: 1129 1129
Size: 4772
Bsp1286I G_dGCh'C
Cuts at: 280 293 1786 2397 2490 2564 2754 2816 3546
Size: 13 1493 611 93 74 190 62 730
Cuts at: 3546 280
Size: 1506
Fragments arranged by size:
1506 1493 730 611 190 93 74 62
13
BspEI T'CCGG_A
Cuts at: 923 3992 923
Size: 3069 1703
BspHI T'CATG_A
Cuts at: 1460 3948 1460
Size: 2488 2284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 201 1822 2036 2338 2719 201
Size: 1621 214 302 381 2254
Fragments arranged by size:
2254 1621 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1301 3064 3118 4602 4767 50
Size: 1251 1763 54 1484 165 55
Fragments arranged by size:
1763 1484 1251 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2684 2684
Size: 4772
BsrFI r'CCGG_y
Cuts at: 2770 2951 3160 2770
Size: 181 209 4382
Fragments arranged by size:
4382 209 181
BssHII G'CGCG_C
Cuts at: 206 226 206
Size: 20 4752
BssSI C'ACGA_G
Cuts at: 1228 3043 3401 1228
Size: 1815 358 2599
Fragments arranged by size:
2599 1815 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2149 2149
Size: 4772
BstBI TT'CG_AA
Cuts at: 110 3133 110
Size: 3023 1749
BstYI r'GATC_y
Cuts at: 1 1490 1797 2264 2287 2622 2868 3880 3966
Size: 1489 307 467 23 335 246 1012 86
Cuts at: 3966 3978 4324 1
Size: 12 346 449
Fragments arranged by size:
1489 1012 467 449 346 335 307 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 199 1356 125
Size: 74 1157 3541
Fragments arranged by size:
3541 1157 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 846 1134 2722 2903 3066 846
Size: 288 1588 181 163 2552
Fragments arranged by size:
2552 1588 288 181 163
BtsI GCAGTG_nn'
Cuts at: 1222 2015 1222
Size: 793 3979
ClaI AT'CG_AT
Cuts at: 980 1268 980
Size: 288 4484
DraI TTT'AAA
Cuts at: 1542 1771 1542
Size: 229 4543
DrdI GACnn_nn'nnGTC
Cuts at: 1081 1456 2478 3336 1081
Size: 375 1022 858 2517
Fragments arranged by size:
2517 1022 858 375
EaeI y'GGCC_r
Cuts at: 545 857 2357 2531 2922 2949 4294 545
Size: 312 1500 174 391 27 1345 1023
Fragments arranged by size:
1500 1345 1023 391 312 174 27
EagI C'GGCC_G
Cuts at: 2357 2357
Size: 4772
EarI CTCTTCn'nnn_
Cuts at: 285 1018 1390 1505 2795 3005 285
Size: 733 372 115 1290 210 2052
Fragments arranged by size:
2052 1290 733 372 210 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2980 3290 3436 4582 2980
Size: 310 146 1146 3170
Fragments arranged by size:
3170 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1784 1784
Size: 4772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 916 1306 2597 3029 3776 4044 567
Size: 349 390 1291 432 747 268 1295
Fragments arranged by size:
1295 1291 747 432 390 349 268
EcoO109I rG'GnC_Cy
Cuts at: 1308 1911 1308
Size: 603 4169
EcoRI G'AATT_C
Cuts at: 402 1776 402
Size: 1374 3398
EcoRV GAT'ATC
Cuts at: 1266 1266
Size: 4772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 4772
FspI TGC'GCA
Cuts at: 2553 2553
Size: 4772
HaeII r_GCGC'y
Cuts at: 133 157 185 1654 2206 2454 3476 4455 133
Size: 24 28 1469 552 248 1022 979 450
Fragments arranged by size:
1469 1022 979 552 450 248 28 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 580 612 1261 1293 1482 1514 2614
Size: 32 247 32 649 32 189 32 1100
Cuts at: 2614 2644 2646 2676 301
Size: 30 2 30 2397
Fragments arranged by size:
2397 1100 649 247 189 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1749 1811 1749
Size: 62 4710
KasI G'GCGC_C
Cuts at: 181 1650 2202 2450 181
Size: 1469 552 248 2503
Fragments arranged by size:
2503 1469 552 248
KpnI G_GTAC'C
Cuts at: 1792 1792
Size: 4772
MluI A'CGCG_T
Cuts at: 4205 4205
Size: 4772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1030 3443 3627 1030
Size: 2413 184 2175
Fragments arranged by size:
2413 2175 184
MscI TGG'CCA
Cuts at: 547 859 2533 547
Size: 312 1674 2786
Fragments arranged by size:
2786 1674 312
MslI CAynn'nnrTG
Cuts at: 271 913 2761 2888 271
Size: 642 1848 127 2155
Fragments arranged by size:
2155 1848 642 127
MspA1I CmG'CkG
Cuts at: 201 248 390 1669 1883 2197 2557 3570 3815
Size: 47 142 1279 214 314 360 1013 245
Cuts at: 3815 201
Size: 1158
Fragments arranged by size:
1279 1158 1013 360 314 245 214 142
47
NaeI GCC'GGC
Cuts at: 2953 2953
Size: 4772
NarI GG'CG_CC
Cuts at: 182 1651 2203 2451 182
Size: 1469 552 248 2503
Fragments arranged by size:
2503 1469 552 248
NcoI C'CATG_G
Cuts at: 125 1356 125
Size: 1231 3541
NgoMIV G'CCGG_C
Cuts at: 2951 2951
Size: 4772
NspI r_CATG'y
Cuts at: 1825 2325 3232 1825
Size: 500 907 3365
Fragments arranged by size:
3365 907 500
PasI CC'CwG_GG
Cuts at: 1563 1563
Size: 4772
PciI A'CATG_T
Cuts at: 3228 3228
Size: 4772
PfoI T'CCnGG_A
Cuts at: 425 425
Size: 4772
PmeI GTTT'AAAC
Cuts at: 1771 1771
Size: 4772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4740
PpuMI rG'GwC_Cy
Cuts at: 1308 1308
Size: 4772
PsiI TTA'TAA
Cuts at: 1187 1187
Size: 4772
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 835 867 835
Size: 32 4740
PstI C_TGCA'G
Cuts at: 389 1529 1819 2504 389
Size: 1140 290 685 2657
Fragments arranged by size:
2657 1140 685 290
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4772
PvuII CAG'CTG
Cuts at: 248 390 1669 2197 2557 248
Size: 142 1279 528 360 2463
Fragments arranged by size:
2463 1279 528 360 142
RsrII CG'GwC_CG
Cuts at: 2967 2967
Size: 4772
SacI G_AGCT'C
Cuts at: 1786 1786
Size: 4772
SacII CC_GC'GG
Cuts at: 202 202
Size: 4772
SalI G'TCGA_C
Cuts at: 1809 1809
Size: 4772
SapI GCTCTTCn'nnn_
Cuts at: 285 1018 1390 1505 2795 3005 285
Size: 733 372 115 1290 210 2052
Fragments arranged by size:
2052 1290 733 372 210 115
SbfI CC_TGCA'GG
Cuts at: 1819 1819
Size: 4772
ScaI AGT'ACT
Cuts at: 4289 4289
Size: 4772
SfcI C'TryA_G
Cuts at: 33 385 776 1525 1815 2500 3493 3684 33
Size: 352 391 749 290 685 993 191 1121
Fragments arranged by size:
1121 993 749 685 391 352 290 191
SfoI GGC'GCC
Cuts at: 183 1652 2204 2452 183
Size: 1469 552 248 2503
Fragments arranged by size:
2503 1469 552 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
SmaI CCC'GGG
Cuts at: 1456 1510 1794 1456
Size: 54 284 4434
Fragments arranged by size:
4434 284 54
SmlI C'TyrA_G
Cuts at: 1752 1927 2842 3334 3596 1752
Size: 175 915 492 262 2928
Fragments arranged by size:
2928 915 492 262 175
SnaBI TAC'GTA
Cuts at: 1764 1764
Size: 4772
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4772
SphI G_CATG'C
Cuts at: 1825 2325 1825
Size: 500 4272
StyI C'CwwG_G
Cuts at: 125 254 571 1356 1906 2260 125
Size: 129 317 785 550 354 2637
Fragments arranged by size:
2637 785 550 354 317 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 374 2355 2488 2947 3156 276
Size: 98 1981 133 459 209 1892
Fragments arranged by size:
1981 1892 459 209 133 98
TatI w'GTAC_w
Cuts at: 4287 4287
Size: 4772
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4289 4321 4289
Size: 32 4740
Tth111I GACn'n_nGTC
Cuts at: 2569 2569
Size: 4772
XbaI T'CTAG_A
Cuts at: 1803 1803
Size: 4772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1015 2267 1015
Size: 1252 3520
XmaI C'CCGG_G
Cuts at: 1454 1508 1792 1454
Size: 54 284 4434
Fragments arranged by size:
4434 284 54
XmnI GAAnn'nnTTC
Cuts at: 366 1399 366
Size: 1033 3739
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PsiI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AlfI AloI ApaI AscI AvrII
BbvCI BfrBI BglI BmtI BseRI BsiWI BsrGI BstEII
BstXI BstZ17I Bsu36I CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PspOMI PspXI SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI