Restriction map for
pF1KSDA1917
(Circular) (Six-base) MAPSORT of: pF1KSDA1917.seq Check: 3386 from: 1 to: 5174
pF1KSDA1917 5174 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1352 1352
Size: 5174
AccI GT'mk_AC
Cuts at: 848 2162 2212 848
Size: 1314 50 3810
Fragments arranged by size:
3810 1314 50
Acc65I G'GTAC_C
Cuts at: 2190 2190
Size: 5174
AclI AA'CG_TT
Cuts at: 5014 5014
Size: 5174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 853 944 1078 1199 1778 2999 3431 4178
Size: 560 91 134 121 579 1221 432 747
Cuts at: 4178 4446 293
Size: 268 1021
Fragments arranged by size:
1221 1021 747 579 560 432 268 134
121 91
AflIII A'CryG_T
Cuts at: 2526 3630 4607 2526
Size: 1104 977 3093
Fragments arranged by size:
3093 1104 977
AgeI A'CCGG_T
Cuts at: 3562 3562
Size: 5174
AhdI GACnn_n'nnGTC
Cuts at: 177 1380 1602 177
Size: 1203 222 3749
Fragments arranged by size:
3749 1203 222
AlwNI CAG_nnn'CTG
Cuts at: 2479 4046 2479
Size: 1567 3607
ApaI G_GGCC'C
Cuts at: 135 1126 1253 1806 2152 135
Size: 991 127 553 346 3157
Fragments arranged by size:
3157 991 553 346 127
ApaLI G'TGCA_C
Cuts at: 3944 3944
Size: 5174
ApoI r'AATT_y
Cuts at: 2178 4527 4783 2178
Size: 2349 256 2569
Fragments arranged by size:
2569 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5174
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5174
AvaI C'yCGr_G
Cuts at: 509 2194 509
Size: 1685 3489
BamHI G'GATC_C
Cuts at: 660 2199 660
Size: 1539 3635
BanI G'GyrC_C
Cuts at: 2047 2190 2604 2852 2887 2047
Size: 143 414 248 35 4334
Fragments arranged by size:
4334 414 248 143 35
BanII G_rGCy'C
Cuts at: 135 445 1126 1253 1806 2152 2188 3218 135
Size: 310 681 127 553 346 36 1030 2091
Fragments arranged by size:
2091 1030 681 553 346 310 127 36
BbeI G_GCGC'C
Cuts at: 2608 2856 2608
Size: 248 4926
BbsI GAAGACnn'nnnn_
Cuts at: 376 932 376
Size: 556 4618
BbvCI CC'TCA_GC
Cuts at: 428 428
Size: 5174
BciVI GTATCCnnnnn_n'
Cuts at: 3072 3833 5174 3072
Size: 761 1341 3072
Fragments arranged by size:
3072 1341 761
BclI T'GATC_A
Cuts at: 2694 2694
Size: 5174
BglI GCCn_nnn'nGGC
Cuts at: 1801 2056 1801
Size: 255 4919
BglII A'GATC_T
Cuts at: 875 2689 4726 875
Size: 1814 2037 1323
Fragments arranged by size:
2037 1814 1323
BlpI GC'TnA_GC
Cuts at: 2286 2286
Size: 5174
Bme1580I G_kGCm'C
Cuts at: 135 1089 1126 1253 1806 2152 2799 2892 3948
Size: 954 37 127 553 346 647 93 1056
Cuts at: 3948 135
Size: 1361
Fragments arranged by size:
1361 1056 954 647 553 346 127 93
37
BmgBI CAC'GTC
Cuts at: 1946 1946
Size: 5174
BmrI ACTGGGnnnn_n'
Cuts at: 1668 2386 2467 2802 4946 1668
Size: 718 81 335 2144 1896
Fragments arranged by size:
2144 1896 718 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1792 1794 3541 1792
Size: 2 1747 3425
Fragments arranged by size:
3425 1747 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 577 1255 1538 1607 1826 1892 2137 467
Size: 110 678 283 69 219 66 245 3504
Fragments arranged by size:
3504 678 283 245 219 110 69 66
Bpu10I CC'TnA_GC
Cuts at: 428 428
Size: 5174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2350 3229 3721 4019 2350
Size: 879 492 298 3505
Fragments arranged by size:
3505 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1506 4878 1506
Size: 3372 1802
BsaAI yAC'GTr
Cuts at: 1287 2166 2527 1287
Size: 879 361 3934
Fragments arranged by size:
3934 879 361
BsaBI GATnn'nnATC
Cuts at: 6 2688 4725 6
Size: 2682 2037 455
Fragments arranged by size:
2682 2037 455
BsaHI Gr'CG_yC
Cuts at: 1618 2605 2853 3555 5051 1618
Size: 987 248 702 1496 1741
Fragments arranged by size:
1741 1496 987 702 248
BsaWI w'CCGG_w
Cuts at: 211 2363 2884 3562 3836 3983 4394 211
Size: 2152 521 678 274 147 411 991
Fragments arranged by size:
2152 991 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1705 1735 1705
Size: 30 5144
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 260 635 697 1713 1777 178
Size: 82 375 62 1016 64 3575
Fragments arranged by size:
3575 1016 375 82 64 62
BseYI C'CCAG_C
Cuts at: 780 1266 1787 1866 2087 2599 3357 3934 4430
Size: 486 521 79 221 512 758 577 496
Cuts at: 4430 780
Size: 1524
Fragments arranged by size:
1524 758 577 521 512 496 486 221
79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1049 628
Size: 421 4753
BsiEI CG_ry'CG
Cuts at: 104 2716 2762 3970 104
Size: 2612 46 1208 1308
Fragments arranged by size:
2612 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2029 2188 2966 3156 3948 2029
Size: 159 778 190 792 3255
Fragments arranged by size:
3255 792 778 190 159
BsmI GAATG_Cn'
Cuts at: 1319 1476 2088 1319
Size: 157 612 4405
Fragments arranged by size:
4405 612 157
BsmBI CGTCTCn'nnnn_
Cuts at: 743 1725 2122 743
Size: 982 397 3795
Fragments arranged by size:
3795 982 397
Bsp1286I G_dGCh'C
Cuts at: 135 445 1089 1126 1253 1806 2029 2152 2188
Size: 310 644 37 127 553 223 123 36
Cuts at: 2188 2799 2892 2966 3156 3218 3948 135
Size: 611 93 74 190 62 730 1361
Fragments arranged by size:
1361 730 644 611 553 310 223 190
127 123 93 74 62 37 36
BspEI T'CCGG_A
Cuts at: 211 4394 211
Size: 4183 991
BspHI T'CATG_A
Cuts at: 4350 4350
Size: 5174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1352 2224 2438 2740 3121 1352
Size: 872 214 302 381 3405
Fragments arranged by size:
3405 872 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3466 3520 5004 5169 50
Size: 3416 54 1484 165 55
Fragments arranged by size:
3416 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 765 3086 765
Size: 2321 2853
BsrFI r'CCGG_y
Cuts at: 143 2093 3172 3353 3562 143
Size: 1950 1079 181 209 1755
Fragments arranged by size:
1950 1755 1079 209 181
BssSI C'ACGA_G
Cuts at: 3445 3803 3445
Size: 358 4816
BstAPI GCAn_nnn'nTGC
Cuts at: 2551 2551
Size: 5174
BstBI TT'CG_AA
Cuts at: 110 3535 110
Size: 3425 1749
BstEII G'GTnAC_C
Cuts at: 277 277
Size: 5174
BstXI CCAn_nnnn'nTGG
Cuts at: 2115 2115
Size: 5174
BstYI r'GATC_y
Cuts at: 1 660 875 1097 2199 2666 2689 3024 3270
Size: 659 215 222 1102 467 23 335 246
Cuts at: 3270 4282 4368 4380 4726 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1102 1012 659 467 449 346 335 246
222 215 86 23 12
BtgI C'CryG_G
Cuts at: 125 539 656 734 1688 125
Size: 414 117 78 954 3611
Fragments arranged by size:
3611 954 414 117 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1728 1977 3124 3305 3468 1728
Size: 249 1147 181 163 3434
Fragments arranged by size:
3434 1147 249 181 163
BtsI GCAGTG_nn'
Cuts at: 1087 1338 2417 1087
Size: 251 1079 3844
Fragments arranged by size:
3844 1079 251
DraI TTT'AAA
Cuts at: 2173 2173
Size: 5174
DraIII CAC_nnn'GTG
Cuts at: 657 657
Size: 5174
DrdI GACnn_nn'nnGTC
Cuts at: 1623 2071 2880 3738 1623
Size: 448 809 858 3059
Fragments arranged by size:
3059 858 809 448
EaeI y'GGCC_r
Cuts at: 762 1299 1338 2759 2933 3324 3351 4696 762
Size: 537 39 1421 174 391 27 1345 1240
Fragments arranged by size:
1421 1345 1240 537 391 174 39 27
EagI C'GGCC_G
Cuts at: 2759 2759
Size: 5174
EarI CTCTTCn'nnn_
Cuts at: 433 446 539 674 1175 1235 1452 1457 1754
Size: 13 93 135 501 60 217 5 297
Cuts at: 1754 1785 1904 3197 3407 433
Size: 31 119 1293 210 2200
Fragments arranged by size:
2200 1293 501 297 217 210 135 119
93 60 31 13 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3382 3692 3838 4984 3382
Size: 310 146 1146 3572
Fragments arranged by size:
3572 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2186 2186
Size: 5174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 467 577 853 944 1078 1199 1255 1538
Size: 174 110 276 91 134 121 56 283
Cuts at: 1538 1607 1778 1826 1892 2137 2999 3431 4178
Size: 69 171 48 66 245 862 432 747
Cuts at: 4178 4446 293
Size: 268 1021
Fragments arranged by size:
1021 862 747 432 283 276 268 245
174 171 134 121 110 91 69 66
56 48
EcoNI CCTnn'n_nnAGG
Cuts at: 868 868
Size: 5174
EcoO109I rG'GnC_Cy
Cuts at: 131 132 1123 1239 1250 1651 1802 1803 2135
Size: 1 991 116 11 401 151 1 332
Cuts at: 2135 2148 2149 2313 131
Size: 13 1 164 2992
Fragments arranged by size:
2992 991 401 332 164 151 116 13
11 1 1 1
EcoRI G'AATT_C
Cuts at: 2178 2178
Size: 5174
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 703 3193 703
Size: 2490 2684
FspI TGC'GCA
Cuts at: 1950 2955 1950
Size: 1005 4169
FspAI rTGC'GCAy
Cuts at: 1950 1950
Size: 5174
HaeII r_GCGC'y
Cuts at: 2094 2100 2608 2856 3878 4857 2094
Size: 6 508 248 1022 979 2411
Fragments arranged by size:
2411 1022 979 508 248 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 1174 1206 1353 1366 1385 1394 1398
Size: 32 979 32 147 13 19 9 4
Cuts at: 1398 1426 1588 1620 1705 1737 1903 1935 3016
Size: 28 162 32 85 32 166 32 1081
Cuts at: 3016 3046 3048 3078 163
Size: 30 2 30 2259
Fragments arranged by size:
2259 1081 979 166 162 147 85 32
32 32 32 32 30 30 28 19
13 9 4 2
HincII GTy'rAC
Cuts at: 2213 2213
Size: 5174
KasI G'GCGC_C
Cuts at: 2604 2852 2604
Size: 248 4926
KpnI G_GTAC'C
Cuts at: 2194 2194
Size: 5174
MluI A'CGCG_T
Cuts at: 4607 4607
Size: 5174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 578 1472 2059 3845 4029 578
Size: 894 587 1786 184 1723
Fragments arranged by size:
1786 1723 894 587 184
MscI TGG'CCA
Cuts at: 764 1301 1340 2935 764
Size: 537 39 1595 3003
Fragments arranged by size:
3003 1595 537 39
MslI CAynn'nnrTG
Cuts at: 479 770 803 1687 3163 3290 479
Size: 291 33 884 1476 127 2363
Fragments arranged by size:
2363 1476 884 291 127 33
MspA1I CmG'CkG
Cuts at: 1834 1870 2285 2599 2959 3972 4217 1834
Size: 36 415 314 360 1013 245 2791
Fragments arranged by size:
2791 1013 415 360 314 245 36
NaeI GCC'GGC
Cuts at: 145 2095 3355 145
Size: 1950 1260 1964
Fragments arranged by size:
1964 1950 1260
NarI GG'CG_CC
Cuts at: 2605 2853 2605
Size: 248 4926
NcoI C'CATG_G
Cuts at: 125 1688 125
Size: 1563 3611
NdeI CA'TA_TG
Cuts at: 1326 1326
Size: 5174
NgoMIV G'CCGG_C
Cuts at: 143 2093 3353 143
Size: 1950 1260 1964
Fragments arranged by size:
1964 1950 1260
NspI r_CATG'y
Cuts at: 733 2227 2727 3634 733
Size: 1494 500 907 2273
Fragments arranged by size:
2273 1494 907 500
PciI A'CATG_T
Cuts at: 3630 3630
Size: 5174
PflMI CCAn_nnn'nTGG
Cuts at: 657 657
Size: 5174
PfoI T'CCnGG_A
Cuts at: 977 977
Size: 5174
PmeI GTTT'AAAC
Cuts at: 2173 2173
Size: 5174
PmlI CAC'GTG
Cuts at: 1287 1287
Size: 5174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 4313
Size: 32 5142
PspOMI G'GGCC_C
Cuts at: 131 1122 1249 1802 2148 131
Size: 991 127 553 346 3157
Fragments arranged by size:
3157 991 553 346 127
PstI C_TGCA'G
Cuts at: 1486 2221 2906 1486
Size: 735 685 3754
Fragments arranged by size:
3754 735 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5174
PvuII CAG'CTG
Cuts at: 1870 2599 2959 1870
Size: 729 360 4085
Fragments arranged by size:
4085 729 360
RsrII CG'GwC_CG
Cuts at: 3369 3369
Size: 5174
SacI G_AGCT'C
Cuts at: 2188 2188
Size: 5174
SalI G'TCGA_C
Cuts at: 2211 2211
Size: 5174
SapI GCTCTTCn'nnn_
Cuts at: 433 1175 3197 3407 433
Size: 742 2022 210 2200
Fragments arranged by size:
2200 2022 742 210
SbfI CC_TGCA'GG
Cuts at: 2221 2221
Size: 5174
ScaI AGT'ACT
Cuts at: 4691 4691
Size: 5174
SexAI A'CCwGG_T
Cuts at: 2043 2043
Size: 5174
SfcI C'TryA_G
Cuts at: 33 1482 2217 2902 3895 4086 33
Size: 1449 735 685 993 191 1121
Fragments arranged by size:
1449 1121 993 735 685 191
SfoI GGC'GCC
Cuts at: 2606 2854 2606
Size: 248 4926
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5174
SgrAI Cr'CCGG_yG
Cuts at: 2093 2093
Size: 5174
SmaI CCC'GGG
Cuts at: 2196 2196
Size: 5174
SmlI C'TyrA_G
Cuts at: 2329 3244 3736 3998 2329
Size: 915 492 262 3505
Fragments arranged by size:
3505 915 492 262
SnaBI TAC'GTA
Cuts at: 2166 2166
Size: 5174
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5174
SphI G_CATG'C
Cuts at: 2227 2727 2227
Size: 500 4674
SspI AAT'ATT
Cuts at: 1203 1203
Size: 5174
StyI C'CwwG_G
Cuts at: 125 1688 2308 2662 125
Size: 1563 620 354 2637
Fragments arranged by size:
2637 1563 620 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 719 1938 2757 2890 3349 3558 719
Size: 1219 819 133 459 209 2335
Fragments arranged by size:
2335 1219 819 459 209 133
TatI w'GTAC_w
Cuts at: 611 774 896 4689 611
Size: 163 122 3793 1096
Fragments arranged by size:
3793 1096 163 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 645 677 4691 4723 645
Size: 32 4014 32 1096
Fragments arranged by size:
4014 1096 32 32
Tth111I GACn'n_nGTC
Cuts at: 853 1717 2971 853
Size: 864 1254 3056
Fragments arranged by size:
3056 1254 864
XbaI T'CTAG_A
Cuts at: 2205 2205
Size: 5174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1665 2669 1665
Size: 1004 4170
XmaI C'CCGG_G
Cuts at: 2194 2194
Size: 5174
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SrfI StuI
SwaI XhoI XmnI ZraI