Restriction map for
pF1KSDA1934
(Circular) (Six-base) MAPSORT of: pF1KSDA1934.seq Check: 8373 from: 1 to: 4481
pF1KSDA1934 4481 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 438 1185 1519 438
Size: 747 334 3400
Fragments arranged by size:
3400 747 334
Acc65I G'GTAC_C
Cuts at: 1497 1497
Size: 4481
AclI AA'CG_TT
Cuts at: 4321 4321
Size: 4481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2306 2738 3485 3753 2306
Size: 432 747 268 3034
Fragments arranged by size:
3034 747 432 268
AflIII A'CryG_T
Cuts at: 1833 2937 3914 1833
Size: 1104 977 2400
Fragments arranged by size:
2400 1104 977
AgeI A'CCGG_T
Cuts at: 2869 2869
Size: 4481
AlwNI CAG_nnn'CTG
Cuts at: 261 1435 1786 3353 261
Size: 1174 351 1567 1389
Fragments arranged by size:
1567 1389 1174 351
ApaLI G'TGCA_C
Cuts at: 946 3251 946
Size: 2305 2176
ApoI r'AATT_y
Cuts at: 227 1485 3834 4090 227
Size: 1258 2349 256 618
Fragments arranged by size:
2349 1258 618 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
AvaI C'yCGr_G
Cuts at: 1007 1501 1007
Size: 494 3987
AvrII C'CTAG_G
Cuts at: 279 279
Size: 4481
BamHI G'GATC_C
Cuts at: 1506 1506
Size: 4481
BanI G'GyrC_C
Cuts at: 1497 1911 2159 2194 1497
Size: 414 248 35 3784
Fragments arranged by size:
3784 414 248 35
BanII G_rGCy'C
Cuts at: 495 929 958 1049 1495 2525 495
Size: 434 29 91 446 1030 2451
Fragments arranged by size:
2451 1030 446 434 91 29
BbeI G_GCGC'C
Cuts at: 1915 2163 1915
Size: 248 4233
BbsI GAAGACnn'nnnn_
Cuts at: 308 312 659 1343 308
Size: 4 347 684 3446
Fragments arranged by size:
3446 684 347 4
BciVI GTATCCnnnnn_n'
Cuts at: 1367 2379 3140 4481 1367
Size: 1012 761 1341 1367
Fragments arranged by size:
1367 1341 1012 761
BclI T'GATC_A
Cuts at: 2001 2001
Size: 4481
BglI GCCn_nnn'nGGC
Cuts at: 690 690
Size: 4481
BglII A'GATC_T
Cuts at: 824 1996 4033 824
Size: 1172 2037 1272
Fragments arranged by size:
2037 1272 1172
BlpI GC'TnA_GC
Cuts at: 1593 1593
Size: 4481
Bme1580I G_kGCm'C
Cuts at: 950 2106 2199 3255 950
Size: 1156 93 1056 2176
Fragments arranged by size:
2176 1156 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 356 1693 1774 2109 4253 356
Size: 1337 81 335 2144 584
Fragments arranged by size:
2144 1337 584 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 918 2848 918
Size: 1930 2551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 754 1063 1102 1364 748
Size: 6 309 39 262 3865
Fragments arranged by size:
3865 309 262 39 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1657 2536 3028 3326 1657
Size: 879 492 298 2812
Fragments arranged by size:
2812 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 637 4185 637
Size: 3548 933
BsaAI yAC'GTr
Cuts at: 739 1355 1473 1834 739
Size: 616 118 361 3386
Fragments arranged by size:
3386 616 361 118
BsaBI GATnn'nnATC
Cuts at: 6 1995 4032 6
Size: 1989 2037 455
Fragments arranged by size:
2037 1989 455
BsaHI Gr'CG_yC
Cuts at: 812 1912 2160 2862 4358 812
Size: 1100 248 702 1496 935
Fragments arranged by size:
1496 1100 935 702 248
BsaWI w'CCGG_w
Cuts at: 1180 1670 2191 2869 3143 3290 3701 1180
Size: 490 521 678 274 147 411 1960
Fragments arranged by size:
1960 678 521 490 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 769 799 884 914 769
Size: 30 85 30 4336
Fragments arranged by size:
4336 85 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1426 1426
Size: 4481
BseYI C'CCAG_C
Cuts at: 1435 1906 2664 3241 3737 1435
Size: 471 758 577 496 2179
Fragments arranged by size:
2179 758 577 496 471
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1199 1252 1199
Size: 53 4428
BsiEI CG_ry'CG
Cuts at: 104 2023 2069 3277 104
Size: 1919 46 1208 1308
Fragments arranged by size:
1919 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 770 950 1049 1495 2273 2463 3255 770
Size: 180 99 446 778 190 792 1996
Fragments arranged by size:
1996 792 778 446 190 180 99
Bsp1286I G_dGCh'C
Cuts at: 495 770 929 950 958 1049 1495 2106 2199
Size: 275 159 21 8 91 446 611 93
Cuts at: 2199 2273 2463 2525 3255 495
Size: 74 190 62 730 1721
Fragments arranged by size:
1721 730 611 446 275 190 159 93
91 74 62 21 8
BspEI T'CCGG_A
Cuts at: 3701 3701
Size: 4481
BspHI T'CATG_A
Cuts at: 3657 3657
Size: 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 657 1531 1745 2047 2428 657
Size: 874 214 302 381 2710
Fragments arranged by size:
2710 874 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2773 2827 4311 4476 50
Size: 2723 54 1484 165 55
Fragments arranged by size:
2723 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2393 2393
Size: 4481
BsrFI r'CCGG_y
Cuts at: 2479 2660 2869 2479
Size: 181 209 4091
Fragments arranged by size:
4091 209 181
BssSI C'ACGA_G
Cuts at: 2752 3110 2752
Size: 358 4123
BstAPI GCAn_nnn'nTGC
Cuts at: 1858 1858
Size: 4481
BstBI TT'CG_AA
Cuts at: 110 2842 110
Size: 2732 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 495 795 1080 495
Size: 300 285 3896
Fragments arranged by size:
3896 300 285
BstYI r'GATC_y
Cuts at: 1 553 824 1506 1973 1996 2331 2577 3589
Size: 552 271 682 467 23 335 246 1012
Cuts at: 3589 3675 3687 4033 1
Size: 86 12 346 449
Fragments arranged by size:
1012 682 552 467 449 346 335 271
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1030 1220 1030
Size: 190 4291
BtgI C'CryG_G
Cuts at: 125 206 1341 125
Size: 81 1135 3265
Fragments arranged by size:
3265 1135 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2431 2612 2775 2431
Size: 181 163 4137
Fragments arranged by size:
4137 181 163
BtsI GCAGTG_nn'
Cuts at: 136 808 1139 1323 1724 136
Size: 672 331 184 401 2893
Fragments arranged by size:
2893 672 401 331 184
DraI TTT'AAA
Cuts at: 1480 1480
Size: 4481
DrdI GACnn_nn'nnGTC
Cuts at: 2187 3045 2187
Size: 858 3623
EaeI y'GGCC_r
Cuts at: 485 1344 2066 2240 2631 2658 4003 485
Size: 859 722 174 391 27 1345 963
Fragments arranged by size:
1345 963 859 722 391 174 27
EagI C'GGCC_G
Cuts at: 2066 2066
Size: 4481
EarI CTCTTCn'nnn_
Cuts at: 635 706 1106 2504 2714 635
Size: 71 400 1398 210 2402
Fragments arranged by size:
2402 1398 400 210 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2689 2999 3145 4291 2689
Size: 310 146 1146 2879
Fragments arranged by size:
2879 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1047 1493 1047
Size: 446 4035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 754 1063 1102 1364 2306 2738 3485 3753
Size: 6 309 39 262 942 432 747 268
Cuts at: 3753 748
Size: 1476
Fragments arranged by size:
1476 942 747 432 309 268 262 39
6
EcoNI CCTnn'n_nnAGG
Cuts at: 632 632
Size: 4481
EcoO109I rG'GnC_Cy
Cuts at: 183 276 1175 1620 183
Size: 93 899 445 3044
Fragments arranged by size:
3044 899 445 93
EcoRI G'AATT_C
Cuts at: 1485 1485
Size: 4481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 267 336 843 975 2500 267
Size: 69 507 132 1525 2248
Fragments arranged by size:
2248 1525 507 132 69
FspI TGC'GCA
Cuts at: 267 970 2262 267
Size: 703 1292 2486
Fragments arranged by size:
2486 1292 703
FspAI rTGC'GCAy
Cuts at: 267 267
Size: 4481
HaeII r_GCGC'y
Cuts at: 1915 2163 3185 4164 1915
Size: 248 1022 979 2232
Fragments arranged by size:
2232 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 884 916 1141 1173 2323 2353 2355 2385 884
Size: 32 225 32 1150 30 2 30 2980
Fragments arranged by size:
2980 1150 225 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1520 1520
Size: 4481
KasI G'GCGC_C
Cuts at: 1911 2159 1911
Size: 248 4233
KpnI G_GTAC'C
Cuts at: 1501 1501
Size: 4481
MluI A'CGCG_T
Cuts at: 3914 3914
Size: 4481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3152 3336 3152
Size: 184 4297
MscI TGG'CCA
Cuts at: 487 1346 2242 487
Size: 859 896 2726
Fragments arranged by size:
2726 896 859
MslI CAynn'nnrTG
Cuts at: 205 763 793 979 2470 2597 205
Size: 558 30 186 1491 127 2089
Fragments arranged by size:
2089 1491 558 186 127 30
MspA1I CmG'CkG
Cuts at: 1592 1906 2266 3279 3524 1592
Size: 314 360 1013 245 2549
Fragments arranged by size:
2549 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2662 2662
Size: 4481
NarI GG'CG_CC
Cuts at: 1912 2160 1912
Size: 248 4233
NcoI C'CATG_G
Cuts at: 125 206 1341 125
Size: 81 1135 3265
Fragments arranged by size:
3265 1135 81
NdeI CA'TA_TG
Cuts at: 688 688
Size: 4481
NgoMIV G'CCGG_C
Cuts at: 2660 2660
Size: 4481
NspI r_CATG'y
Cuts at: 1534 2034 2941 1534
Size: 500 907 3074
Fragments arranged by size:
3074 907 500
PasI CC'CwG_GG
Cuts at: 495 1460 495
Size: 965 3516
PciI A'CATG_T
Cuts at: 2937 2937
Size: 4481
PflMI CCAn_nnn'nTGG
Cuts at: 1460 1460
Size: 4481
PmeI GTTT'AAAC
Cuts at: 1480 1480
Size: 4481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4449
PpuMI rG'GwC_Cy
Cuts at: 276 276
Size: 4481
PstI C_TGCA'G
Cuts at: 875 938 1407 1528 2213 875
Size: 63 469 121 685 3143
Fragments arranged by size:
3143 685 469 121 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4481
PvuII CAG'CTG
Cuts at: 1906 2266 1906
Size: 360 4121
RsrII CG'GwC_CG
Cuts at: 2676 2676
Size: 4481
SacI G_AGCT'C
Cuts at: 1049 1495 1049
Size: 446 4035
SalI G'TCGA_C
Cuts at: 1518 1518
Size: 4481
SapI GCTCTTCn'nnn_
Cuts at: 2504 2714 2504
Size: 210 4271
SbfI CC_TGCA'GG
Cuts at: 1528 1528
Size: 4481
ScaI AGT'ACT
Cuts at: 3998 3998
Size: 4481
SfcI C'TryA_G
Cuts at: 33 871 934 1403 1524 2209 3202 3393 33
Size: 838 63 469 121 685 993 191 1121
Fragments arranged by size:
1121 993 838 685 469 191 121 63
SfoI GGC'GCC
Cuts at: 1913 2161 1913
Size: 248 4233
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
SmaI CCC'GGG
Cuts at: 1503 1503
Size: 4481
SmlI C'TyrA_G
Cuts at: 1636 2551 3043 3305 1636
Size: 915 492 262 2812
Fragments arranged by size:
2812 915 492 262
SnaBI TAC'GTA
Cuts at: 1473 1473
Size: 4481
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4481
SphI G_CATG'C
Cuts at: 1534 2034 1534
Size: 500 3981
StyI C'CwwG_G
Cuts at: 125 206 279 311 1341 1361 1615 1969 125
Size: 81 73 32 1030 20 254 354 2637
Fragments arranged by size:
2637 1030 354 254 81 73 32 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2064 2197 2656 2865 2064
Size: 133 459 209 3680
Fragments arranged by size:
3680 459 209 133
TatI w'GTAC_w
Cuts at: 3996 3996
Size: 4481
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 4449
Tth111I GACn'n_nGTC
Cuts at: 340 2278 340
Size: 1938 2543
XbaI T'CTAG_A
Cuts at: 1512 1512
Size: 4481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1976 1976
Size: 4481
XmaI C'CCGG_G
Cuts at: 1501 1501
Size: 4481
XmnI GAAnn'nnTTC
Cuts at: 448 621 448
Size: 173 4308
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI ZraI