Restriction map for
pF1KSDA1942
(Circular) (Six-base) MAPSORT of: pF1KSDA1942.seq Check: 4978 from: 1 to: 4475
pF1KSDA1942 4475 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 669 669
Size: 4475
AatII G_ACGT'C
Cuts at: 1058 1058
Size: 4475
AccI GT'mk_AC
Cuts at: 221 1463 1513 221
Size: 1242 50 3183
Fragments arranged by size:
3183 1242 50
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2300 2732 3479 3747 1396
Size: 904 432 747 268 2124
Fragments arranged by size:
2124 904 747 432 268
AflIII A'CryG_T
Cuts at: 154 1827 2931 3908 154
Size: 1673 1104 977 721
Fragments arranged by size:
1673 1104 977 721
AgeI A'CCGG_T
Cuts at: 805 820 2863 805
Size: 15 2043 2417
Fragments arranged by size:
2417 2043 15
AleI CACnn'nnGTG
Cuts at: 572 572
Size: 4475
AlwNI CAG_nnn'CTG
Cuts at: 702 1344 1347 1780 3347 702
Size: 642 3 433 1567 1830
Fragments arranged by size:
1830 1567 642 433 3
ApaI G_GGCC'C
Cuts at: 211 1119 211
Size: 908 3567
ApaLI G'TGCA_C
Cuts at: 1354 3245 1354
Size: 1891 2584
ApoI r'AATT_y
Cuts at: 1479 3828 4084 1479
Size: 2349 256 1870
Fragments arranged by size:
2349 1870 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 797 1239 1495 797
Size: 442 256 3777
Fragments arranged by size:
3777 442 256
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 313 561 1208 1391 1491 1905 2153 2188 313
Size: 248 647 183 100 414 248 35 2600
Fragments arranged by size:
2600 647 414 248 248 183 100 35
BanII G_rGCy'C
Cuts at: 211 695 1088 1119 1412 1489 2519 211
Size: 484 393 31 293 77 1030 2167
Fragments arranged by size:
2167 1030 484 393 293 77 31
BbeI G_GCGC'C
Cuts at: 317 1909 2157 317
Size: 1592 248 2635
Fragments arranged by size:
2635 1592 248
BbvCI CC'TCA_GC
Cuts at: 542 542
Size: 4475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 760 794 760
Size: 34 4441
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3134 4475 2373
Size: 761 1341 2373
Fragments arranged by size:
2373 1341 761
BclI T'GATC_A
Cuts at: 1995 1995
Size: 4475
BglII A'GATC_T
Cuts at: 1124 1990 4027 1124
Size: 866 2037 1572
Fragments arranged by size:
2037 1572 866
BlpI GC'TnA_GC
Cuts at: 966 1587 966
Size: 621 3854
Bme1580I G_kGCm'C
Cuts at: 211 566 1119 1358 1405 2100 2193 3249 211
Size: 355 553 239 47 695 93 1056 1437
Fragments arranged by size:
1437 1056 695 553 355 239 93 47
BmgBI CAC'GTC
Cuts at: 1305 1305
Size: 4475
BmrI ACTGGGnnnn_n'
Cuts at: 1153 1687 1768 2103 4247 1153
Size: 534 81 335 2144 1381
Fragments arranged by size:
2144 1381 534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1092 568
Size: 524 3951
Bpu10I CC'TnA_GC
Cuts at: 542 542
Size: 4475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1651 2530 3022 3320 1105
Size: 546 879 492 298 2260
Fragments arranged by size:
2260 879 546 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1360 4179 1360
Size: 2819 1656
BsaAI yAC'GTr
Cuts at: 155 879 1182 1467 1828 155
Size: 724 303 285 361 2802
Fragments arranged by size:
2802 724 361 303 285
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 147 314 974 1055 1906 2154 2856 4352 147
Size: 167 660 81 851 248 702 1496 270
Fragments arranged by size:
1496 851 702 660 270 248 167 81
BsaWI w'CCGG_w
Cuts at: 362 805 820 1664 2185 2863 3137 3284 3695
Size: 443 15 844 521 678 274 147 411
Cuts at: 3695 362
Size: 1142
Fragments arranged by size:
1142 844 678 521 443 411 274 147
15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 853 883 853
Size: 30 4445
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 292 520 703 274
Size: 18 228 183 4046
Fragments arranged by size:
4046 228 183 18
BseYI C'CCAG_C
Cuts at: 397 603 622 1009 1900 2658 3235 3731 397
Size: 206 19 387 891 758 577 496 1141
Fragments arranged by size:
1141 891 758 577 496 387 206 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1265 1369 1265
Size: 104 4371
BsiEI CG_ry'CG
Cuts at: 104 234 810 2017 2063 3271 104
Size: 130 576 1207 46 1208 1308
Fragments arranged by size:
1308 1208 1207 576 130 46
BsiHKAI G_wGCw'C
Cuts at: 293 1358 1489 2267 2457 3249 293
Size: 1065 131 778 190 792 1519
Fragments arranged by size:
1519 1065 792 778 190 131
Bsp1286I G_dGCh'C
Cuts at: 211 293 566 695 1088 1119 1358 1405 1412
Size: 82 273 129 393 31 239 47 7
Cuts at: 1412 1489 2100 2193 2267 2457 2519 3249 211
Size: 77 611 93 74 190 62 730 1437
Fragments arranged by size:
1437 730 611 393 273 239 190 129
93 82 77 74 62 47 31 7
BspEI T'CCGG_A
Cuts at: 3695 3695
Size: 4475
BspHI T'CATG_A
Cuts at: 3651 3651
Size: 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 669 932 1525 1739 2041 2422 233
Size: 436 263 593 214 302 381 2286
Fragments arranged by size:
2286 593 436 381 302 263 214
BsrBI CCG'CTC
Cuts at: 50 534 1284 2767 2821 4305 4470 50
Size: 484 750 1483 54 1484 165 55
Fragments arranged by size:
1484 1483 750 484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 805 820 2473 2654 2863 805
Size: 15 1653 181 209 2417
Fragments arranged by size:
2417 1653 209 181 15
BssHII G'CGCG_C
Cuts at: 133 133
Size: 4475
BssSI C'ACGA_G
Cuts at: 2746 3104 2746
Size: 358 4117
BstAPI GCAn_nnn'nTGC
Cuts at: 456 1019 1344 1852 456
Size: 563 325 508 3079
Fragments arranged by size:
3079 563 508 325
BstBI TT'CG_AA
Cuts at: 110 2836 110
Size: 2726 1749
BstEII G'GTnAC_C
Cuts at: 801 932 801
Size: 131 4344
BstXI CCAn_nnnn'nTGG
Cuts at: 574 574
Size: 4475
BstYI r'GATC_y
Cuts at: 1 1124 1500 1967 1990 2325 2571 3583 3669
Size: 1123 376 467 23 335 246 1012 86
Cuts at: 3669 3681 4027 1
Size: 12 346 449
Fragments arranged by size:
1123 1012 467 449 376 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1292 1292
Size: 4475
BtgI C'CryG_G
Cuts at: 125 173 557 125
Size: 48 384 4043
Fragments arranged by size:
4043 384 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 495 934 1283 1405 1718 495
Size: 439 349 122 313 3252
Fragments arranged by size:
3252 439 349 313 122
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DraIII CAC_nnn'GTG
Cuts at: 1265 1265
Size: 4475
DrdI GACnn_nn'nnGTC
Cuts at: 1060 1225 2181 3039 1060
Size: 165 956 858 2496
Fragments arranged by size:
2496 956 858 165
EaeI y'GGCC_r
Cuts at: 231 554 889 1163 1325 2060 2234 2625 2652
Size: 323 335 274 162 735 174 391 27
Cuts at: 2652 3997 231
Size: 1345 709
Fragments arranged by size:
1345 735 709 391 335 323 274 174
162 27
EagI C'GGCC_G
Cuts at: 231 2060 231
Size: 1829 2646
EarI CTCTTCn'nnn_
Cuts at: 497 512 524 605 2498 2708 497
Size: 15 12 81 1893 210 2264
Fragments arranged by size:
2264 1893 210 81 15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2683 2993 3139 4285 2683
Size: 310 146 1146 2873
Fragments arranged by size:
2873 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1487 1487
Size: 4475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1092 1396 2300 2732 3479 3747 568
Size: 524 304 904 432 747 268 1296
Fragments arranged by size:
1296 904 747 524 432 304 268
EcoO109I rG'GnC_Cy
Cuts at: 207 401 699 921 1115 1116 1131 1269 1287
Size: 194 298 222 194 1 15 138 18
Cuts at: 1287 1614 207
Size: 327 3068
Fragments arranged by size:
3068 327 298 222 194 194 138 18
15 1
EcoRI G'AATT_C
Cuts at: 1479 1479
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 762 2494 762
Size: 1732 2743
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 317 419 1429 1909 2157 3179 4158 317
Size: 102 1010 480 248 1022 979 634
Fragments arranged by size:
1022 1010 979 634 480 248 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 438 470 853 885 2317 2347 2349 2379 438
Size: 32 383 32 1432 30 2 30 2534
Fragments arranged by size:
2534 1432 383 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1514 1514
Size: 4475
KasI G'GCGC_C
Cuts at: 313 1905 2153 313
Size: 1592 248 2635
Fragments arranged by size:
2635 1592 248
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3146 3330 3146
Size: 184 4291
MscI TGG'CCA
Cuts at: 556 1165 2236 556
Size: 609 1071 2795
Fragments arranged by size:
2795 1071 609
MslI CAynn'nnrTG
Cuts at: 572 2464 2591 572
Size: 1892 127 2456
Fragments arranged by size:
2456 1892 127
MspA1I CmG'CkG
Cuts at: 546 888 970 1070 1223 1344 1586 1900 2260
Size: 342 82 100 153 121 242 314 360
Cuts at: 2260 3273 3518 546
Size: 1013 245 1503
Fragments arranged by size:
1503 1013 360 342 314 245 242 153
121 100 82
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 314 1906 2154 314
Size: 1592 248 2635
Fragments arranged by size:
2635 1592 248
NcoI C'CATG_G
Cuts at: 125 173 557 125
Size: 48 384 4043
Fragments arranged by size:
4043 384 48
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NspI r_CATG'y
Cuts at: 1026 1528 2028 2935 1026
Size: 502 500 907 2566
Fragments arranged by size:
2566 907 502 500
PasI CC'CwG_GG
Cuts at: 1405 1405
Size: 4475
PciI A'CATG_T
Cuts at: 2931 2931
Size: 4475
PflMI CCAn_nnn'nTGG
Cuts at: 702 702
Size: 4475
PfoI T'CCnGG_A
Cuts at: 341 716 341
Size: 375 4100
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PmlI CAC'GTG
Cuts at: 155 879 1182 155
Size: 724 303 3448
Fragments arranged by size:
3448 724 303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4443
PpuMI rG'GwC_Cy
Cuts at: 401 921 1131 1269 1287 401
Size: 520 210 138 18 3589
Fragments arranged by size:
3589 520 210 138 18
PshAI GACnn'nnGTC
Cuts at: 1198 1198
Size: 4475
PspOMI G'GGCC_C
Cuts at: 207 1115 207
Size: 908 3567
PstI C_TGCA'G
Cuts at: 680 929 1522 2207 680
Size: 249 593 685 2948
Fragments arranged by size:
2948 685 593 249
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 546 970 1070 1344 1900 2260 546
Size: 424 100 274 556 360 2761
Fragments arranged by size:
2761 556 424 360 274 100
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 1489 1489
Size: 4475
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SanDI GG'GwC_CC
Cuts at: 401 1287 401
Size: 886 3589
SapI GCTCTTCn'nnn_
Cuts at: 524 605 2498 2708 524
Size: 81 1893 210 2291
Fragments arranged by size:
2291 1893 210 81
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SfcI C'TryA_G
Cuts at: 33 676 925 1518 2203 3196 3387 33
Size: 643 249 593 685 993 191 1121
Fragments arranged by size:
1121 993 685 643 593 249 191
SfoI GGC'GCC
Cuts at: 315 1907 2155 315
Size: 1592 248 2635
Fragments arranged by size:
2635 1592 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 799 1497 799
Size: 698 3777
SmlI C'TyrA_G
Cuts at: 1120 1630 2545 3037 3299 1120
Size: 510 915 492 262 2296
Fragments arranged by size:
2296 915 510 492 262
SnaBI TAC'GTA
Cuts at: 1467 1467
Size: 4475
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1026 1528 2028 1026
Size: 502 500 3473
Fragments arranged by size:
3473 502 500
StuI AGG'CCT
Cuts at: 283 1018 283
Size: 735 3740
StyI C'CwwG_G
Cuts at: 125 173 557 1609 1963 125
Size: 48 384 1052 354 2637
Fragments arranged by size:
2637 1052 384 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 595 1384 2058 2191 2650 2859 595
Size: 789 674 133 459 209 2211
Fragments arranged by size:
2211 789 674 459 209 133
TatI w'GTAC_w
Cuts at: 3990 3990
Size: 4475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 913 945 3992 4024 913
Size: 32 3047 32 1364
Fragments arranged by size:
3047 1364 32 32
Tth111I GACn'n_nGTC
Cuts at: 337 2272 337
Size: 1935 2540
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1970 1970
Size: 4475
XmaI C'CCGG_G
Cuts at: 797 1495 797
Size: 698 3777
ZraI GAC'GTC
Cuts at: 1056 1056
Size: 4475
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BfrBI BglI BmtI BsiWI BsmI BsmBI BsrGI
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SrfI
SspI SwaI XhoI XmnI