Restriction map for
pF1KSDA1949
(Circular) (Six-base) MAPSORT of: pF1KSDA1949.seq Check: 8669 from: 1 to: 4976
pF1KSDA1949 4976 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 789 2014 789
Size: 1225 3751
Acc65I G'GTAC_C
Cuts at: 1640 1992 1640
Size: 352 4624
AclI AA'CG_TT
Cuts at: 4816 4816
Size: 4976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1734 2801 3233 3980 4248 1734
Size: 1067 432 747 268 2462
Fragments arranged by size:
2462 1067 747 432 268
AflII C'TTAA_G
Cuts at: 1738 1738
Size: 4976
AflIII A'CryG_T
Cuts at: 2328 3432 4409 2328
Size: 1104 977 2895
Fragments arranged by size:
2895 1104 977
AgeI A'CCGG_T
Cuts at: 3364 3364
Size: 4976
AlwNI CAG_nnn'CTG
Cuts at: 2281 3848 2281
Size: 1567 3409
ApaI G_GGCC'C
Cuts at: 1518 1819 1518
Size: 301 4675
ApaLI G'TGCA_C
Cuts at: 379 3746 379
Size: 3367 1609
ApoI r'AATT_y
Cuts at: 1980 4329 4585 1980
Size: 2349 256 2371
Fragments arranged by size:
2371 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4976
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4976
AvaI C'yCGr_G
Cuts at: 482 632 752 1148 1620 1996 482
Size: 150 120 396 472 376 3462
Fragments arranged by size:
3462 472 396 376 150 120
BamHI G'GATC_C
Cuts at: 1461 2001 1461
Size: 540 4436
BanI G'GyrC_C
Cuts at: 167 1640 1992 2406 2654 2689 167
Size: 1473 352 414 248 35 2454
Fragments arranged by size:
2454 1473 414 352 248 35
BanII G_rGCy'C
Cuts at: 254 526 603 676 799 1281 1302 1394 1518
Size: 272 77 73 123 482 21 92 124
Cuts at: 1518 1819 1913 1990 3020 254
Size: 301 94 77 1030 2210
Fragments arranged by size:
2210 1030 482 301 272 124 123 94
92 77 77 73 21
BbeI G_GCGC'C
Cuts at: 171 2410 2658 171
Size: 2239 248 2489
Fragments arranged by size:
2489 2239 248
BbvCI CC'TCA_GC
Cuts at: 913 913
Size: 4976
BciVI GTATCCnnnnn_n'
Cuts at: 2874 3635 4976 2874
Size: 761 1341 2874
Fragments arranged by size:
2874 1341 761
BclI T'GATC_A
Cuts at: 2496 2496
Size: 4976
BglII A'GATC_T
Cuts at: 1658 1742 2491 4528 1658
Size: 84 749 2037 2106
Fragments arranged by size:
2106 2037 749 84
BlpI GC'TnA_GC
Cuts at: 2088 2088
Size: 4976
Bme1580I G_kGCm'C
Cuts at: 383 1325 1518 1568 1819 1842 2601 2694 3750
Size: 942 193 50 251 23 759 93 1056
Cuts at: 3750 383
Size: 1609
Fragments arranged by size:
1609 1056 942 759 251 193 93 50
23
BmrI ACTGGGnnnn_n'
Cuts at: 1829 2188 2269 2604 4748 1829
Size: 359 81 335 2144 2057
Fragments arranged by size:
2144 2057 359 335 81
BmtI G_CTAG'C
Cuts at: 163 454 163
Size: 291 4685
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3343 3343
Size: 4976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 317 382 496 646 660 707 734 843
Size: 40 65 114 150 14 47 27 109
Cuts at: 843 913 1184 1265 1376 1378 1580 1786 1825
Size: 70 271 81 111 2 202 206 39
Cuts at: 1825 1835 1897 277
Size: 10 62 3356
Fragments arranged by size:
3356 271 206 202 150 114 111 109
81 70 65 62 47 40 39 27
14 10 2
Bpu10I CC'TnA_GC
Cuts at: 913 1280 913
Size: 367 4609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2152 3031 3523 3821 2152
Size: 879 492 298 3307
Fragments arranged by size:
3307 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 486 562 843 1009 1762 4680 486
Size: 76 281 166 753 2918 782
Fragments arranged by size:
2918 782 753 281 166 76
BsaAI yAC'GTr
Cuts at: 1775 1968 2329 1775
Size: 193 361 4422
Fragments arranged by size:
4422 361 193
BsaBI GATnn'nnATC
Cuts at: 6 2490 4527 6
Size: 2484 2037 455
Fragments arranged by size:
2484 2037 455
BsaHI Gr'CG_yC
Cuts at: 168 184 2407 2655 3357 4853 168
Size: 16 2223 248 702 1496 291
Fragments arranged by size:
2223 1496 702 291 248 16
BsaWI w'CCGG_w
Cuts at: 2165 2686 3364 3638 3785 4196 2165
Size: 521 678 274 147 411 2945
Fragments arranged by size:
2945 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 199 208 236 238 266 658 688 693
Size: 30 9 28 2 28 392 30 5
Cuts at: 693 723 1759 1789 169
Size: 30 1036 30 3356
Fragments arranged by size:
3356 1036 392 30 30 30 30 28
28 9 5 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 594 1282 1669 1822 193
Size: 401 688 387 153 3347
Fragments arranged by size:
3347 688 401 387 153
BseYI C'CCAG_C
Cuts at: 496 585 1348 1431 1578 1627 1812 2401 3159
Size: 89 763 83 147 49 185 589 758
Cuts at: 3159 3736 4232 496
Size: 577 496 1240
Fragments arranged by size:
1240 763 758 589 577 496 185 147
89 83 49
BsiEI CG_ry'CG
Cuts at: 104 2518 2564 3772 104
Size: 2414 46 1208 1308
Fragments arranged by size:
2414 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 383 676 1394 1913 1990 2768 2958 3750 383
Size: 293 718 519 77 778 190 792 1609
Fragments arranged by size:
1609 792 778 718 519 293 190 77
BsmBI CGTCTCn'nnnn_
Cuts at: 1690 1690
Size: 4976
Bsp1286I G_dGCh'C
Cuts at: 254 383 526 603 676 799 1281 1302 1325
Size: 129 143 77 73 123 482 21 23
Cuts at: 1325 1394 1518 1568 1819 1842 1913 1990 2601
Size: 69 124 50 251 23 71 77 611
Cuts at: 2601 2694 2768 2958 3020 3750 254
Size: 93 74 190 62 730 1480
Fragments arranged by size:
1480 730 611 482 251 190 143 129
124 123 93 77 77 74 73 71
69 62 50 23 23 21
BspEI T'CCGG_A
Cuts at: 4196 4196
Size: 4976
BspHI T'CATG_A
Cuts at: 1469 4152 1469
Size: 2683 2293
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2026 2240 2542 2923 2026
Size: 214 302 381 4079
Fragments arranged by size:
4079 381 302 214
BsrBI CCG'CTC
Cuts at: 50 408 489 729 3268 3322 4806 4971 50
Size: 358 81 240 2539 54 1484 165 55
Fragments arranged by size:
2539 1484 358 240 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 2888 2888
Size: 4976
BsrFI r'CCGG_y
Cuts at: 170 1958 2974 3155 3364 170
Size: 1788 1016 181 209 1782
Fragments arranged by size:
1788 1782 1016 209 181
BssSI C'ACGA_G
Cuts at: 3247 3605 3247
Size: 358 4618
BstAPI GCAn_nnn'nTGC
Cuts at: 2353 2353
Size: 4976
BstBI TT'CG_AA
Cuts at: 110 3337 110
Size: 3227 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1456 1456
Size: 4976
BstYI r'GATC_y
Cuts at: 1 1461 1658 1742 2001 2468 2491 2826 3072
Size: 1460 197 84 259 467 23 335 246
Cuts at: 3072 4084 4170 4182 4528 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1460 1012 467 449 346 335 259 246
197 86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 616 1826 616
Size: 1210 3766
BtgI C'CryG_G
Cuts at: 125 125
Size: 4976
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2926 3107 3270 2926
Size: 181 163 4632
Fragments arranged by size:
4632 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 1533 2219 1391
Size: 142 686 4148
Fragments arranged by size:
4148 686 142
DraI TTT'AAA
Cuts at: 1975 1975
Size: 4976
DrdI GACnn_nn'nnGTC
Cuts at: 2682 3540 2682
Size: 858 4118
EaeI y'GGCC_r
Cuts at: 128 970 2561 2735 3126 3153 4498 128
Size: 842 1591 174 391 27 1345 606
Fragments arranged by size:
1591 1345 842 606 391 174 27
EagI C'GGCC_G
Cuts at: 2561 2561
Size: 4976
EarI CTCTTCn'nnn_
Cuts at: 437 528 542 959 1370 1868 1880 2999 3209
Size: 91 14 417 411 498 12 1119 210
Cuts at: 3209 437
Size: 2204
Fragments arranged by size:
2204 1119 498 417 411 210 91 14
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3184 3494 3640 4786 3184
Size: 310 146 1146 3374
Fragments arranged by size:
3374 1146 310 146
EcoICRI GAG'CTC
Cuts at: 674 1392 1911 1988 674
Size: 718 519 77 3662
Fragments arranged by size:
3662 718 519 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 317 382 496 646 660 707 734 843
Size: 40 65 114 150 14 47 27 109
Cuts at: 843 913 1184 1265 1376 1378 1580 1734 1786
Size: 70 271 81 111 2 202 154 52
Cuts at: 1786 1825 1835 1897 2801 3233 3980 4248 277
Size: 39 10 62 904 432 747 268 1005
Fragments arranged by size:
1005 904 747 432 271 268 202 154
150 114 111 109 81 70 65 62
52 47 40 39 27 14 10 2
EcoNI CCTnn'n_nnAGG
Cuts at: 308 361 832 1705 308
Size: 53 471 873 3579
Fragments arranged by size:
3579 873 471 53
EcoO109I rG'GnC_Cy
Cuts at: 1514 1515 1712 1830 1935 2115 1514
Size: 1 197 118 105 180 4375
Fragments arranged by size:
4375 197 180 118 105 1
EcoRI G'AATT_C
Cuts at: 1980 1980
Size: 4976
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1764 2995 1764
Size: 1231 3745
FspI TGC'GCA
Cuts at: 1927 2757 1927
Size: 830 4146
HaeII r_GCGC'y
Cuts at: 171 222 264 2410 2658 3680 4659 171
Size: 51 42 2146 248 1022 979 488
Fragments arranged by size:
2146 1022 979 488 248 51 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 208 240 501 533 543 575 658
Size: 32 7 32 261 32 10 32 83
Cuts at: 658 690 693 725 2818 2848 2850 2880 169
Size: 32 3 32 2093 30 2 30 2265
Fragments arranged by size:
2265 2093 261 83 32 32 32 32
32 32 30 30 10 7 3 2
HincII GTy'rAC
Cuts at: 1548 2015 1548
Size: 467 4509
HindIII A'AGCT_T
Cuts at: 274 1216 274
Size: 942 4034
KasI G'GCGC_C
Cuts at: 167 2406 2654 167
Size: 2239 248 2489
Fragments arranged by size:
2489 2239 248
KpnI G_GTAC'C
Cuts at: 1644 1996 1644
Size: 352 4624
MluI A'CGCG_T
Cuts at: 4409 4409
Size: 4976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3647 3831 3647
Size: 184 4792
MscI TGG'CCA
Cuts at: 130 972 2737 130
Size: 842 1765 2369
Fragments arranged by size:
2369 1765 842
MslI CAynn'nnrTG
Cuts at: 696 2965 3092 696
Size: 2269 127 2580
Fragments arranged by size:
2580 2269 127
MspA1I CmG'CkG
Cuts at: 156 1697 2087 2401 2761 3774 4019 156
Size: 1541 390 314 360 1013 245 1113
Fragments arranged by size:
1541 1113 1013 390 360 314 245
NaeI GCC'GGC
Cuts at: 172 1960 3157 172
Size: 1788 1197 1991
Fragments arranged by size:
1991 1788 1197
NarI GG'CG_CC
Cuts at: 168 2407 2655 168
Size: 2239 248 2489
Fragments arranged by size:
2489 2239 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4976
NgoMIV G'CCGG_C
Cuts at: 170 1958 3155 170
Size: 1788 1197 1991
Fragments arranged by size:
1991 1788 1197
NheI G'CTAG_C
Cuts at: 159 450 159
Size: 291 4685
NspI r_CATG'y
Cuts at: 2029 2529 3436 2029
Size: 500 907 3569
Fragments arranged by size:
3569 907 500
PasI CC'CwG_GG
Cuts at: 289 502 799 1163 289
Size: 213 297 364 4102
Fragments arranged by size:
4102 364 297 213
PciI A'CATG_T
Cuts at: 3432 3432
Size: 4976
PflMI CCAn_nnn'nTGG
Cuts at: 822 822
Size: 4976
PfoI T'CCnGG_A
Cuts at: 254 711 1138 254
Size: 457 427 4092
Fragments arranged by size:
4092 457 427
PmeI GTTT'AAAC
Cuts at: 1975 1975
Size: 4976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1300 1332 4115 4147 1300
Size: 32 2783 32 2129
Fragments arranged by size:
2783 2129 32 32
PpuMI rG'GwC_Cy
Cuts at: 1712 1830 1712
Size: 118 4858
PspOMI G'GGCC_C
Cuts at: 1514 1815 1514
Size: 301 4675
PstI C_TGCA'G
Cuts at: 1005 1307 1617 1904 2023 2708 1005
Size: 302 310 287 119 685 3273
Fragments arranged by size:
3273 685 310 302 287 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4976
PvuII CAG'CTG
Cuts at: 156 1697 2401 2761 156
Size: 1541 704 360 2371
Fragments arranged by size:
2371 1541 704 360
RsrII CG'GwC_CG
Cuts at: 3171 3171
Size: 4976
SacI G_AGCT'C
Cuts at: 676 1394 1913 1990 676
Size: 718 519 77 3662
Fragments arranged by size:
3662 718 519 77
SalI G'TCGA_C
Cuts at: 2013 2013
Size: 4976
SanDI GG'GwC_CC
Cuts at: 1712 1712
Size: 4976
SapI GCTCTTCn'nnn_
Cuts at: 437 1880 2999 3209 437
Size: 1443 1119 210 2204
Fragments arranged by size:
2204 1443 1119 210
SbfI CC_TGCA'GG
Cuts at: 2023 2023
Size: 4976
ScaI AGT'ACT
Cuts at: 4493 4493
Size: 4976
SfcI C'TryA_G
Cuts at: 33 151 317 1001 1303 1613 1900 2019 2704
Size: 118 166 684 302 310 287 119 685
Cuts at: 2704 3697 3888 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 684 310 302 287 191
166 119 118
SfoI GGC'GCC
Cuts at: 169 2408 2656 169
Size: 2239 248 2489
Fragments arranged by size:
2489 2239 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4976
SmaI CCC'GGG
Cuts at: 484 1622 1998 484
Size: 1138 376 3462
Fragments arranged by size:
3462 1138 376
SmlI C'TyrA_G
Cuts at: 752 1148 1738 2131 3046 3538 3800 752
Size: 396 590 393 915 492 262 1928
Fragments arranged by size:
1928 915 590 492 396 393 262
SnaBI TAC'GTA
Cuts at: 1968 1968
Size: 4976
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4976
SphI G_CATG'C
Cuts at: 2029 2529 2029
Size: 500 4476
StuI AGG'CCT
Cuts at: 621 1077 1290 1361 621
Size: 456 213 71 4236
Fragments arranged by size:
4236 456 213 71
StyI C'CwwG_G
Cuts at: 125 527 1035 1706 1931 2110 2464 125
Size: 402 508 671 225 179 354 2637
Fragments arranged by size:
2637 671 508 402 354 225 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1176 2559 2692 3151 3360 1176
Size: 1383 133 459 209 2792
Fragments arranged by size:
2792 1383 459 209 133
TatI w'GTAC_w
Cuts at: 4491 4491
Size: 4976
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4493 4525 4493
Size: 32 4944
Tth111I GACn'n_nGTC
Cuts at: 557 2773 557
Size: 2216 2760
XbaI T'CTAG_A
Cuts at: 2007 2007
Size: 4976
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1115 2471 1115
Size: 1356 3620
XhoI C'TCGA_G
Cuts at: 752 1148 752
Size: 396 4580
XmaI C'CCGG_G
Cuts at: 482 1620 1996 482
Size: 1138 376 3462
Fragments arranged by size:
3462 1138 376
XmnI GAAnn'nnTTC
Cuts at: 391 391
Size: 4976
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SrfI SspI SwaI ZraI