Restriction map for
pF1KSDA1951
(Circular) (Six-base) MAPSORT of: pF1KSDA1951.seq Check: 1507 from: 1 to: 5147
pF1KSDA1951 5147 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1449 1604 1449
Size: 155 4992
AccI GT'mk_AC
Cuts at: 1214 2185 1214
Size: 971 4176
Acc65I G'GTAC_C
Cuts at: 2163 2163
Size: 5147
AclI AA'CG_TT
Cuts at: 4987 4987
Size: 5147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2972 3404 4151 4419 2972
Size: 432 747 268 3700
Fragments arranged by size:
3700 747 432 268
AfeI AGC'GCT
Cuts at: 1732 1732
Size: 5147
AflIII A'CryG_T
Cuts at: 2499 3603 4580 2499
Size: 1104 977 3066
Fragments arranged by size:
3066 1104 977
AgeI A'CCGG_T
Cuts at: 3535 3535
Size: 5147
AleI CACnn'nnGTG
Cuts at: 1122 1249 1508 1544 1610 1637 1122
Size: 127 259 36 66 27 4632
Fragments arranged by size:
4632 259 127 66 36 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 383 635 1526 1612 383
Size: 252 891 86 3918
Fragments arranged by size:
3918 891 252 86
AlwNI CAG_nnn'CTG
Cuts at: 567 2046 2452 4019 567
Size: 1479 406 1567 1695
Fragments arranged by size:
1695 1567 1479 406
ApaI G_GGCC'C
Cuts at: 446 1104 1269 1526 446
Size: 658 165 257 4067
Fragments arranged by size:
4067 658 257 165
ApaLI G'TGCA_C
Cuts at: 1697 1998 3917 1697
Size: 301 1919 2927
Fragments arranged by size:
2927 1919 301
ApoI r'AATT_y
Cuts at: 2151 4500 4756 2151
Size: 2349 256 2542
Fragments arranged by size:
2542 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5147
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5147
AvaI C'yCGr_G
Cuts at: 580 1913 2167 580
Size: 1333 254 3560
Fragments arranged by size:
3560 1333 254
BamHI G'GATC_C
Cuts at: 2172 2172
Size: 5147
BanI G'GyrC_C
Cuts at: 426 775 1368 1502 2163 2577 2825 2860 426
Size: 349 593 134 661 414 248 35 2713
Fragments arranged by size:
2713 661 593 414 349 248 134 35
BanII G_rGCy'C
Cuts at: 446 494 713 942 987 1104 1269 1412 1526
Size: 48 219 229 45 117 165 143 114
Cuts at: 1526 1747 1920 2111 2161 3191 446
Size: 221 173 191 50 1030 2402
Fragments arranged by size:
2402 1030 229 221 219 191 173 165
143 117 114 50 48 45
BbeI G_GCGC'C
Cuts at: 2581 2829 2581
Size: 248 4899
BbsI GAAGACnn'nnnn_
Cuts at: 1137 1137
Size: 5147
BbvCI CC'TCA_GC
Cuts at: 143 437 975 1482 1669 143
Size: 294 538 507 187 3621
Fragments arranged by size:
3621 538 507 294 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1276 1310 1276
Size: 34 5113
BciVI GTATCCnnnnn_n'
Cuts at: 3045 3806 5147 3045
Size: 761 1341 3045
Fragments arranged by size:
3045 1341 761
BclI T'GATC_A
Cuts at: 2667 2667
Size: 5147
BglII A'GATC_T
Cuts at: 2662 4699 2662
Size: 2037 3110
BlpI GC'TnA_GC
Cuts at: 632 2259 632
Size: 1627 3520
Bme1580I G_kGCm'C
Cuts at: 446 778 1104 1269 1371 1526 1701 2002 2772
Size: 332 326 165 102 155 175 301 770
Cuts at: 2772 2865 3921 446
Size: 93 1056 1672
Fragments arranged by size:
1672 1056 770 332 326 301 175 165
155 102 93
BmgBI CAC'GTC
Cuts at: 1702 1702
Size: 5147
BmrI ACTGGGnnnn_n'
Cuts at: 2095 2359 2440 2775 4919 2095
Size: 264 81 335 2144 2323
Fragments arranged by size:
2323 2144 335 264 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3514 3514
Size: 5147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 433 1385 1958 352
Size: 81 952 573 3541
Fragments arranged by size:
3541 952 573 81
Bpu10I CC'TnA_GC
Cuts at: 143 437 941 975 1482 1669 143
Size: 294 504 34 507 187 3621
Fragments arranged by size:
3621 507 504 294 187 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2074 2323 3202 3694 3992 1186
Size: 888 249 879 492 298 2341
Fragments arranged by size:
2341 888 879 492 298 249
BsaI GGTCTCn'nnnn_
Cuts at: 1828 2008 4851 1828
Size: 180 2843 2124
Fragments arranged by size:
2843 2124 180
BsaAI yAC'GTr
Cuts at: 1584 2139 2500 1584
Size: 555 361 4231
Fragments arranged by size:
4231 555 361
BsaBI GATnn'nnATC
Cuts at: 6 1950 2661 4698 6
Size: 1944 711 2037 455
Fragments arranged by size:
2037 1944 711 455
BsaHI Gr'CG_yC
Cuts at: 1536 2578 2826 3528 5024 1536
Size: 1042 248 702 1496 1659
Fragments arranged by size:
1659 1496 1042 702 248
BsaWI w'CCGG_w
Cuts at: 2336 2857 3535 3809 3956 4367 2336
Size: 521 678 274 147 411 3116
Fragments arranged by size:
3116 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 772 802 772
Size: 30 5117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 397 769 817 835 838 856 892 394
Size: 3 372 48 18 3 18 36 4649
Fragments arranged by size:
4649 372 48 36 18 18 3 3
BseYI C'CCAG_C
Cuts at: 551 608 935 962 1395 1445 2007 2039 2572
Size: 57 327 27 433 50 562 32 533
Cuts at: 2572 3330 3907 4403 551
Size: 758 577 496 1295
Fragments arranged by size:
1295 758 577 562 533 496 433 327
57 50 32 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 2066 1532
Size: 534 4613
BsiEI CG_ry'CG
Cuts at: 104 2689 2735 3943 104
Size: 2585 46 1208 1308
Fragments arranged by size:
2585 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 304 494 733 1468 1701 1747 1920 2002 2161
Size: 190 239 735 233 46 173 82 159
Cuts at: 2161 2939 3129 3921 304
Size: 778 190 792 1530
Fragments arranged by size:
1530 792 778 735 239 233 190 190
173 159 82 46
BsmI GAATG_Cn'
Cuts at: 466 466
Size: 5147
Bsp1286I G_dGCh'C
Cuts at: 304 446 494 713 733 778 942 987 1104
Size: 142 48 219 20 45 164 45 117
Cuts at: 1104 1269 1371 1412 1468 1526 1701 1747 1920
Size: 165 102 41 56 58 175 46 173
Cuts at: 1920 2002 2111 2161 2772 2865 2939 3129 3191
Size: 82 109 50 611 93 74 190 62
Cuts at: 3191 3921 304
Size: 730 1530
Fragments arranged by size:
1530 730 611 219 190 175 173 165
164 142 117 109 102 93 82 74
62 58 56 50 48 46 45 45
41 20
BspEI T'CCGG_A
Cuts at: 4367 4367
Size: 5147
BspHI T'CATG_A
Cuts at: 4323 4323
Size: 5147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1141 1449 1604 1760 2197 2411 2713 3094 1141
Size: 308 155 156 437 214 302 381 3194
Fragments arranged by size:
3194 437 381 308 302 214 156 155
BsrBI CCG'CTC
Cuts at: 50 3439 3493 4977 5142 50
Size: 3389 54 1484 165 55
Fragments arranged by size:
3389 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 816 1282 3059 816
Size: 466 1777 2904
Fragments arranged by size:
2904 1777 466
BsrFI r'CCGG_y
Cuts at: 989 998 1079 1259 1340 1354 1505 1763 3145
Size: 9 81 180 81 14 151 258 1382
Cuts at: 3145 3326 3535 989
Size: 181 209 2601
Fragments arranged by size:
2601 1382 258 209 181 180 151 81
81 14 9
BsrGI T'GTAC_A
Cuts at: 1126 1126
Size: 5147
BssSI C'ACGA_G
Cuts at: 3418 3776 3418
Size: 358 4789
BstAPI GCAn_nnn'nTGC
Cuts at: 363 1176 2524 363
Size: 813 1348 2986
Fragments arranged by size:
2986 1348 813
BstBI TT'CG_AA
Cuts at: 110 3508 110
Size: 3398 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1111 1639 1111
Size: 528 4619
BstYI r'GATC_y
Cuts at: 1 536 2172 2639 2662 2997 3243 4255 4341
Size: 535 1636 467 23 335 246 1012 86
Cuts at: 4341 4353 4699 1
Size: 12 346 449
Fragments arranged by size:
1636 1012 535 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 752 752
Size: 5147
BtgI C'CryG_G
Cuts at: 125 884 1224 1877 125
Size: 759 340 653 3395
Fragments arranged by size:
3395 759 653 340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1145 1171 1273 2004 3097 3278 3441 1145
Size: 26 102 731 1093 181 163 2851
Fragments arranged by size:
2851 1093 731 181 163 102 26
BtsI GCAGTG_nn'
Cuts at: 188 670 1041 1271 2390 188
Size: 482 371 230 1119 2945
Fragments arranged by size:
2945 1119 482 371 230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 635 670 635
Size: 35 5112
DraI TTT'AAA
Cuts at: 2146 2146
Size: 5147
DrdI GACnn_nn'nnGTC
Cuts at: 2853 3711 2853
Size: 858 4289
EaeI y'GGCC_r
Cuts at: 149 1221 1661 1780 1860 2732 2906 3297 3324
Size: 1072 440 119 80 872 174 391 27
Cuts at: 3324 4669 149
Size: 1345 627
Fragments arranged by size:
1345 1072 872 627 440 391 174 119
80 27
EagI C'GGCC_G
Cuts at: 2732 2732
Size: 5147
EarI CTCTTCn'nnn_
Cuts at: 280 824 857 3170 3380 280
Size: 544 33 2313 210 2047
Fragments arranged by size:
2313 2047 544 210 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1017 1062 3355 3665 3811 4957 1017
Size: 45 2293 310 146 1146 1207
Fragments arranged by size:
2293 1207 1146 310 146 45
EcoICRI GAG'CTC
Cuts at: 492 1745 1918 2159 492
Size: 1253 173 241 3480
Fragments arranged by size:
3480 1253 241 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 433 1385 1958 2972 3404 4151 4419 352
Size: 81 952 573 1014 432 747 268 1080
Fragments arranged by size:
1080 1014 952 747 573 432 268 81
EcoNI CCTnn'n_nnAGG
Cuts at: 1376 1376
Size: 5147
EcoO109I rG'GnC_Cy
Cuts at: 258 442 443 1523 1619 2286 258
Size: 184 1 1080 96 667 3119
Fragments arranged by size:
3119 1080 667 184 96 1
EcoRI G'AATT_C
Cuts at: 2151 2151
Size: 5147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3166 3166
Size: 5147
FspI TGC'GCA
Cuts at: 1588 2928 1588
Size: 1340 3807
HaeII r_GCGC'y
Cuts at: 1734 2053 2581 2829 3851 4830 1734
Size: 319 528 248 1022 979 2051
Fragments arranged by size:
2051 1022 979 528 319 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2989 3019 3021 3051 2989
Size: 30 2 30 5085
Fragments arranged by size:
5085 30 30 2
HincII GTy'rAC
Cuts at: 194 227 2186 194
Size: 33 1959 3155
Fragments arranged by size:
3155 1959 33
KasI G'GCGC_C
Cuts at: 2577 2825 2577
Size: 248 4899
KpnI G_GTAC'C
Cuts at: 2167 2167
Size: 5147
MluI A'CGCG_T
Cuts at: 4580 4580
Size: 5147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 797 1772 2099 3818 4002 797
Size: 975 327 1719 184 1942
Fragments arranged by size:
1942 1719 975 327 184
MscI TGG'CCA
Cuts at: 1663 2908 1663
Size: 1245 3902
MslI CAynn'nnrTG
Cuts at: 506 1109 1122 1249 1508 1544 1610 1637 1694
Size: 603 13 127 259 36 66 27 57
Cuts at: 1694 3136 3263 506
Size: 1442 127 2390
Fragments arranged by size:
2390 1442 603 259 127 127 66 57
36 27 13
MspA1I CmG'CkG
Cuts at: 931 1226 1405 1449 1544 1683 1865 1902 2070
Size: 295 179 44 95 139 182 37 168
Cuts at: 2070 2258 2572 2932 3945 4190 931
Size: 188 314 360 1013 245 1888
Fragments arranged by size:
1888 1013 360 314 295 245 188 182
179 168 139 95 44 37
NaeI GCC'GGC
Cuts at: 991 1356 3328 991
Size: 365 1972 2810
Fragments arranged by size:
2810 1972 365
NarI GG'CG_CC
Cuts at: 2578 2826 2578
Size: 248 4899
NcoI C'CATG_G
Cuts at: 125 884 1877 125
Size: 759 993 3395
Fragments arranged by size:
3395 993 759
NgoMIV G'CCGG_C
Cuts at: 989 1354 3326 989
Size: 365 1972 2810
Fragments arranged by size:
2810 1972 365
NspI r_CATG'y
Cuts at: 366 2200 2700 3607 366
Size: 1834 500 907 1906
Fragments arranged by size:
1906 1834 907 500
PasI CC'CwG_GG
Cuts at: 484 484
Size: 5147
PciI A'CATG_T
Cuts at: 3603 3603
Size: 5147
PflMI CCAn_nnn'nTGG
Cuts at: 1716 2046 1716
Size: 330 4817
PmeI GTTT'AAAC
Cuts at: 2146 2146
Size: 5147
PmlI CAC'GTG
Cuts at: 1584 1584
Size: 5147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 4286
Size: 32 5115
PspOMI G'GGCC_C
Cuts at: 442 1100 1265 1522 442
Size: 658 165 257 4067
Fragments arranged by size:
4067 658 257 165
PstI C_TGCA'G
Cuts at: 1048 1088 1757 2069 2194 2879 1048
Size: 40 669 312 125 685 3316
Fragments arranged by size:
3316 685 669 312 125 40
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5147
PvuII CAG'CTG
Cuts at: 931 1449 1683 2070 2572 2932 931
Size: 518 234 387 502 360 3146
Fragments arranged by size:
3146 518 502 387 360 234
RsrII CG'GwC_CG
Cuts at: 3342 3342
Size: 5147
SacI G_AGCT'C
Cuts at: 494 1747 1920 2161 494
Size: 1253 173 241 3480
Fragments arranged by size:
3480 1253 241 173
SacII CC_GC'GG
Cuts at: 1227 1227
Size: 5147
SalI G'TCGA_C
Cuts at: 2184 2184
Size: 5147
SapI GCTCTTCn'nnn_
Cuts at: 3170 3380 3170
Size: 210 4937
SbfI CC_TGCA'GG
Cuts at: 2194 2194
Size: 5147
ScaI AGT'ACT
Cuts at: 4664 4664
Size: 5147
SfcI C'TryA_G
Cuts at: 33 1044 1084 1753 1933 2065 2190 2875 3868
Size: 1011 40 669 180 132 125 685 993
Cuts at: 3868 4059 33
Size: 191 1121
Fragments arranged by size:
1121 1011 993 685 669 191 180 132
125 40
SfoI GGC'GCC
Cuts at: 2579 2827 2579
Size: 248 4899
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5147
SgrAI Cr'CCGG_yG
Cuts at: 998 1259 1340 1505 1763 998
Size: 261 81 165 258 4382
Fragments arranged by size:
4382 261 258 165 81
SmaI CCC'GGG
Cuts at: 2169 2169
Size: 5147
SmlI C'TyrA_G
Cuts at: 1165 2053 2302 3217 3709 3971 1165
Size: 888 249 915 492 262 2341
Fragments arranged by size:
2341 915 888 492 262 249
SnaBI TAC'GTA
Cuts at: 2139 2139
Size: 5147
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5147
SphI G_CATG'C
Cuts at: 2200 2700 2200
Size: 500 4647
StyI C'CwwG_G
Cuts at: 125 884 1139 1877 2281 2635 125
Size: 759 255 738 404 354 2637
Fragments arranged by size:
2637 759 738 404 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 579 793 1045 2730 2863 3322 3531 579
Size: 214 252 1685 133 459 209 2195
Fragments arranged by size:
2195 1685 459 252 214 209 133
TatI w'GTAC_w
Cuts at: 1126 1515 4662 1126
Size: 389 3147 1611
Fragments arranged by size:
3147 1611 389
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 772 804 4664 4696 772
Size: 32 3860 32 1223
Fragments arranged by size:
3860 1223 32 32
Tth111I GACn'n_nGTC
Cuts at: 2944 2944
Size: 5147
XbaI T'CTAG_A
Cuts at: 2178 2178
Size: 5147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 2642 132
Size: 2510 2637
XmaI C'CCGG_G
Cuts at: 2167 2167
Size: 5147
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BglI BmtI BsiWI BsmBI BssHII BstEII BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SrfI SspI
StuI SwaI XhoI XmnI ZraI