Restriction map for
pF1KSDA1956
(Circular) (Six-base) MAPSORT of: pF1KSDA1956.seq Check: 3788 from: 1 to: 5165
pF1KSDA1956 5165 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 337 337
Size: 5165
AccI GT'mk_AC
Cuts at: 2203 2203
Size: 5165
Acc65I G'GTAC_C
Cuts at: 2181 2181
Size: 5165
AclI AA'CG_TT
Cuts at: 5005 5005
Size: 5165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2004 2990 3422 4169 4437 2004
Size: 986 432 747 268 2732
Fragments arranged by size:
2732 986 747 432 268
AflIII A'CryG_T
Cuts at: 771 2517 3621 4598 771
Size: 1746 1104 977 1338
Fragments arranged by size:
1746 1338 1104 977
AgeI A'CCGG_T
Cuts at: 3553 3553
Size: 5165
AleI CACnn'nnGTG
Cuts at: 374 458 1220 374
Size: 84 762 4319
Fragments arranged by size:
4319 762 84
AlwNI CAG_nnn'CTG
Cuts at: 450 2470 4037 450
Size: 2020 1567 1578
Fragments arranged by size:
2020 1578 1567
ApaLI G'TGCA_C
Cuts at: 3935 3935
Size: 5165
ApoI r'AATT_y
Cuts at: 1843 2169 4518 4774 1843
Size: 326 2349 256 2234
Fragments arranged by size:
2349 2234 326 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5165
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
AvaI C'yCGr_G
Cuts at: 1967 2185 1967
Size: 218 4947
BamHI G'GATC_C
Cuts at: 2190 2190
Size: 5165
BanI G'GyrC_C
Cuts at: 288 1273 2181 2595 2843 2878 288
Size: 985 908 414 248 35 2575
Fragments arranged by size:
2575 985 908 414 248 35
BanII G_rGCy'C
Cuts at: 1777 2179 3209 1777
Size: 402 1030 3733
Fragments arranged by size:
3733 1030 402
BbeI G_GCGC'C
Cuts at: 2599 2847 2599
Size: 248 4917
BciVI GTATCCnnnnn_n'
Cuts at: 3063 3824 5165 3063
Size: 761 1341 3063
Fragments arranged by size:
3063 1341 761
BclI T'GATC_A
Cuts at: 2685 2685
Size: 5165
BfrBI ATG'CAT
Cuts at: 749 749
Size: 5165
BglII A'GATC_T
Cuts at: 2680 4717 2680
Size: 2037 3128
BlpI GC'TnA_GC
Cuts at: 2277 2277
Size: 5165
Bme1580I G_kGCm'C
Cuts at: 136 1276 2790 2883 3939 136
Size: 1140 1514 93 1056 1362
Fragments arranged by size:
1514 1362 1140 1056 93
BmgBI CAC'GTC
Cuts at: 222 222
Size: 5165
BmrI ACTGGGnnnn_n'
Cuts at: 246 680 893 977 2377 2458 2793 4937 246
Size: 434 213 84 1400 81 335 2144 474
Fragments arranged by size:
2144 1400 474 434 335 213 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 5165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1073 1157 1409 1493 1577 1661 1745 1836
Size: 823 84 252 84 84 84 84 91
Cuts at: 1836 1904 2072 250
Size: 68 168 3343
Fragments arranged by size:
3343 823 252 168 91 84 84 84
84 84 68
Bpu10I CC'TnA_GC
Cuts at: 639 639
Size: 5165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2341 3220 3712 4010 2341
Size: 879 492 298 3496
Fragments arranged by size:
3496 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4869 4869
Size: 5165
BsaAI yAC'GTr
Cuts at: 774 2157 2518 774
Size: 1383 361 3421
Fragments arranged by size:
3421 1383 361
BsaBI GATnn'nnATC
Cuts at: 6 2679 4716 6
Size: 2673 2037 455
Fragments arranged by size:
2673 2037 455
BsaHI Gr'CG_yC
Cuts at: 2596 2844 3546 5042 2596
Size: 248 702 1496 2719
Fragments arranged by size:
2719 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2354 2875 3553 3827 3974 4385 2354
Size: 521 678 274 147 411 3134
Fragments arranged by size:
3134 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 298 607 190
Size: 108 309 4748
Fragments arranged by size:
4748 309 108
BseYI C'CCAG_C
Cuts at: 634 821 2590 3348 3925 4421 634
Size: 187 1769 758 577 496 1378
Fragments arranged by size:
1769 1378 758 577 496 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1848 2007 2091 436
Size: 1412 159 84 3510
Fragments arranged by size:
3510 1412 159 84
BsiEI CG_ry'CG
Cuts at: 104 2707 2753 3961 104
Size: 2603 46 1208 1308
Fragments arranged by size:
2603 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1777 2179 2957 3147 3939 1777
Size: 402 778 190 792 3003
Fragments arranged by size:
3003 792 778 402 190
BsmI GAATG_Cn'
Cuts at: 751 1906 751
Size: 1155 4010
Bsp1286I G_dGCh'C
Cuts at: 136 1276 1777 2179 2790 2883 2957 3147 3209
Size: 1140 501 402 611 93 74 190 62
Cuts at: 3209 3939 136
Size: 730 1362
Fragments arranged by size:
1362 1140 730 611 501 402 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4385 4385
Size: 5165
BspHI T'CATG_A
Cuts at: 4341 4341
Size: 5165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 337 2215 2429 2731 3112 337
Size: 1878 214 302 381 2390
Fragments arranged by size:
2390 1878 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3457 3511 4995 5160 50
Size: 3407 54 1484 165 55
Fragments arranged by size:
3407 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3077 3077
Size: 5165
BsrFI r'CCGG_y
Cuts at: 3163 3344 3553 3163
Size: 181 209 4775
Fragments arranged by size:
4775 209 181
BssSI C'ACGA_G
Cuts at: 3436 3794 3436
Size: 358 4807
BstAPI GCAn_nnn'nTGC
Cuts at: 450 2542 450
Size: 2092 3073
BstBI TT'CG_AA
Cuts at: 110 3526 110
Size: 3416 1749
BstYI r'GATC_y
Cuts at: 1 2190 2657 2680 3015 3261 4273 4359 4371
Size: 2189 467 23 335 246 1012 86 12
Cuts at: 4371 4717 1
Size: 346 449
Fragments arranged by size:
2189 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1363 1363
Size: 5165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3115 3296 3459 3115
Size: 181 163 4821
Fragments arranged by size:
4821 181 163
BtsI GCAGTG_nn'
Cuts at: 549 1805 2079 2408 549
Size: 1256 274 329 3306
Fragments arranged by size:
3306 1256 329 274
DraI TTT'AAA
Cuts at: 1947 2164 1947
Size: 217 4948
DrdI GACnn_nn'nnGTC
Cuts at: 2871 3729 2871
Size: 858 4307
EaeI y'GGCC_r
Cuts at: 2750 2924 3315 3342 4687 2750
Size: 174 391 27 1345 3228
Fragments arranged by size:
3228 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2750 2750
Size: 5165
EarI CTCTTCn'nnn_
Cuts at: 295 562 1782 2109 3188 3398 295
Size: 267 1220 327 1079 210 2062
Fragments arranged by size:
2062 1220 1079 327 267 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3373 3683 3829 4975 3373
Size: 310 146 1146 3563
Fragments arranged by size:
3563 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1775 2177 1775
Size: 402 4763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1073 1157 1409 1493 1577 1661 1745 1836
Size: 823 84 252 84 84 84 84 91
Cuts at: 1836 1904 2004 2072 2990 3422 4169 4437 250
Size: 68 100 68 918 432 747 268 978
Fragments arranged by size:
978 918 823 747 432 268 252 100
91 84 84 84 84 84 68 68
EcoNI CCTnn'n_nnAGG
Cuts at: 193 344 193
Size: 151 5014
EcoO109I rG'GnC_Cy
Cuts at: 2304 2304
Size: 5165
EcoRI G'AATT_C
Cuts at: 2169 2169
Size: 5165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2162 3184 2162
Size: 1022 4143
FspI TGC'GCA
Cuts at: 1940 2946 1940
Size: 1006 4159
HaeII r_GCGC'y
Cuts at: 2599 2847 3869 4848 2599
Size: 248 1022 979 2916
Fragments arranged by size:
2916 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 583 615 688 720 3007 3037 3039 3069 583
Size: 32 73 32 2287 30 2 30 2679
Fragments arranged by size:
2679 2287 73 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2204 2204
Size: 5165
KasI G'GCGC_C
Cuts at: 2595 2843 2595
Size: 248 4917
KpnI G_GTAC'C
Cuts at: 2185 2185
Size: 5165
MluI A'CGCG_T
Cuts at: 4598 4598
Size: 5165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3836 4020 3836
Size: 184 4981
MscI TGG'CCA
Cuts at: 2926 2926
Size: 5165
MslI CAynn'nnrTG
Cuts at: 374 458 1220 1572 3154 3281 374
Size: 84 762 352 1582 127 2258
Fragments arranged by size:
2258 1582 762 352 127 84
MspA1I CmG'CkG
Cuts at: 737 2276 2590 2950 3963 4208 737
Size: 1539 314 360 1013 245 1694
Fragments arranged by size:
1694 1539 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3346 3346
Size: 5165
NarI GG'CG_CC
Cuts at: 2596 2844 2596
Size: 248 4917
NdeI CA'TA_TG
Cuts at: 1569 1569
Size: 5165
NgoMIV G'CCGG_C
Cuts at: 3344 3344
Size: 5165
NsiI A_TGCA'T
Cuts at: 751 751
Size: 5165
NspI r_CATG'y
Cuts at: 2218 2718 3625 2218
Size: 500 907 3758
Fragments arranged by size:
3758 907 500
PasI CC'CwG_GG
Cuts at: 255 255
Size: 5165
PciI A'CATG_T
Cuts at: 3621 3621
Size: 5165
PmeI GTTT'AAAC
Cuts at: 2164 2164
Size: 5165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 4304
Size: 32 5133
PspXI vC'TCGA_Gb
Cuts at: 1967 1967
Size: 5165
PstI C_TGCA'G
Cuts at: 656 1812 2212 2897 656
Size: 1156 400 685 2924
Fragments arranged by size:
2924 1156 685 400
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5165
PvuII CAG'CTG
Cuts at: 737 2590 2950 737
Size: 1853 360 2952
Fragments arranged by size:
2952 1853 360
RsrII CG'GwC_CG
Cuts at: 3360 3360
Size: 5165
SacI G_AGCT'C
Cuts at: 1777 2179 1777
Size: 402 4763
SalI G'TCGA_C
Cuts at: 2202 2202
Size: 5165
SapI GCTCTTCn'nnn_
Cuts at: 295 1782 2109 3188 3398 295
Size: 1487 327 1079 210 2062
Fragments arranged by size:
2062 1487 1079 327 210
SbfI CC_TGCA'GG
Cuts at: 2212 2212
Size: 5165
ScaI AGT'ACT
Cuts at: 4682 4682
Size: 5165
SfcI C'TryA_G
Cuts at: 33 534 652 1808 2208 2893 3886 4077 33
Size: 501 118 1156 400 685 993 191 1121
Fragments arranged by size:
1156 1121 993 685 501 400 191 118
SfoI GGC'GCC
Cuts at: 2597 2845 2597
Size: 248 4917
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
SmaI CCC'GGG
Cuts at: 2187 2187
Size: 5165
SmlI C'TyrA_G
Cuts at: 1967 2320 3235 3727 3989 1967
Size: 353 915 492 262 3143
Fragments arranged by size:
3143 915 492 353 262
SnaBI TAC'GTA
Cuts at: 2157 2157
Size: 5165
SpeI A'CTAG_T
Cuts at: 67 1219 67
Size: 1152 4013
SphI G_CATG'C
Cuts at: 2218 2718 2218
Size: 500 4665
StuI AGG'CCT
Cuts at: 1482 1734 1893 2061 1482
Size: 252 159 168 4586
Fragments arranged by size:
4586 252 168 159
StyI C'CwwG_G
Cuts at: 519 2299 2653 519
Size: 1780 354 3031
Fragments arranged by size:
3031 1780 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2748 2881 3340 3549 2748
Size: 133 459 209 4364
Fragments arranged by size:
4364 459 209 133
TatI w'GTAC_w
Cuts at: 2043 4680 2043
Size: 2637 2528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4682 4714 4682
Size: 32 5133
Tth111I GACn'n_nGTC
Cuts at: 2962 2962
Size: 5165
XbaI T'CTAG_A
Cuts at: 802 2196 802
Size: 1394 3771
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2660 2660
Size: 5165
XhoI C'TCGA_G
Cuts at: 1967 1967
Size: 5165
XmaI C'CCGG_G
Cuts at: 2185 2185
Size: 5165
XmnI GAAnn'nnTTC
Cuts at: 1846 2103 1846
Size: 257 4908
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PasI PciI PmeI PpiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI PacI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI ZraI