Restriction map for
pF1KSDA1957
(Circular) (Six-base) MAPSORT of: pF1KSDA1957.seq Check: 3354 from: 1 to: 4400
pF1KSDA1957 4400 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1109 1109
Size: 4400
AatII G_ACGT'C
Cuts at: 565 565
Size: 4400
AccI GT'mk_AC
Cuts at: 1438 1438
Size: 4400
Acc65I G'GTAC_C
Cuts at: 1416 1416
Size: 4400
AclI AA'CG_TT
Cuts at: 4240 4240
Size: 4400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 741 2225 2657 3404 3672 457
Size: 284 1484 432 747 268 1185
Fragments arranged by size:
1484 1185 747 432 284 268
AflIII A'CryG_T
Cuts at: 140 1752 2856 3833 140
Size: 1612 1104 977 707
Fragments arranged by size:
1612 1104 977 707
AgeI A'CCGG_T
Cuts at: 2788 2788
Size: 4400
AhdI GACnn_n'nnGTC
Cuts at: 486 780 486
Size: 294 4106
AleI CACnn'nnGTG
Cuts at: 1210 1210
Size: 4400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 448 448
Size: 4400
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 604 636 604
Size: 32 4368
AlwNI CAG_nnn'CTG
Cuts at: 357 1705 3272 357
Size: 1348 1567 1485
Fragments arranged by size:
1567 1485 1348
ApaI G_GGCC'C
Cuts at: 233 304 337 715 735 1039 1271 233
Size: 71 33 378 20 304 232 3362
Fragments arranged by size:
3362 378 304 232 71 33 20
ApaLI G'TGCA_C
Cuts at: 1096 3170 1096
Size: 2074 2326
ApoI r'AATT_y
Cuts at: 1404 3753 4009 1404
Size: 2349 256 1795
Fragments arranged by size:
2349 1795 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4400
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
AvaI C'yCGr_G
Cuts at: 188 297 946 994 1040 1345 1420 188
Size: 109 649 48 46 305 75 3168
Fragments arranged by size:
3168 649 305 109 75 48 46
BamHI G'GATC_C
Cuts at: 1425 1425
Size: 4400
BanI G'GyrC_C
Cuts at: 526 546 671 956 975 1176 1416 1830 2078
Size: 20 125 285 19 201 240 414 248
Cuts at: 2078 2113 526
Size: 35 2813
Fragments arranged by size:
2813 414 285 248 240 201 125 35
20 19
BanII G_rGCy'C
Cuts at: 233 304 337 394 595 642 658 715 735
Size: 71 33 57 201 47 16 57 20
Cuts at: 735 745 1039 1271 1414 2444 233
Size: 10 294 232 143 1030 2189
Fragments arranged by size:
2189 1030 294 232 201 143 71 57
57 47 33 20 16 10
BbeI G_GCGC'C
Cuts at: 675 960 1834 2082 675
Size: 285 874 248 2993
Fragments arranged by size:
2993 874 285 248
BbsI GAAGACnn'nnnn_
Cuts at: 1227 1227
Size: 4400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1191 1225 1191
Size: 34 4366
BciVI GTATCCnnnnn_n'
Cuts at: 2298 3059 4400 2298
Size: 761 1341 2298
Fragments arranged by size:
2298 1341 761
BclI T'GATC_A
Cuts at: 1920 1920
Size: 4400
BglI GCCn_nnn'nGGC
Cuts at: 367 412 583 905 1043 1081 367
Size: 45 171 322 138 38 3686
Fragments arranged by size:
3686 322 171 138 45 38
BglII A'GATC_T
Cuts at: 1915 3952 1915
Size: 2037 2363
BlpI GC'TnA_GC
Cuts at: 1512 1512
Size: 4400
Bme1580I G_kGCm'C
Cuts at: 233 304 337 551 715 735 978 1039 1100
Size: 71 33 214 164 20 243 61 61
Cuts at: 1100 1271 2025 2118 3174 233
Size: 171 754 93 1056 1459
Fragments arranged by size:
1459 1056 754 243 214 171 164 93
71 61 61 33 20
BmgBI CAC'GTC
Cuts at: 344 344
Size: 4400
BmrI ACTGGGnnnn_n'
Cuts at: 1612 1693 2028 4172 1612
Size: 81 335 2144 1840
Fragments arranged by size:
2144 1840 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 4400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 1576 2455 2947 3245 1312
Size: 264 879 492 298 2467
Fragments arranged by size:
2467 879 492 298 264
BsaI GGTCTCn'nnnn_
Cuts at: 4104 4104
Size: 4400
BsaAI yAC'GTr
Cuts at: 456 1032 1392 1753 456
Size: 576 360 361 3103
Fragments arranged by size:
3103 576 361 360
BsaBI GATnn'nnATC
Cuts at: 6 463 1914 3951 6
Size: 457 1451 2037 455
Fragments arranged by size:
2037 1451 457 455
BsaHI Gr'CG_yC
Cuts at: 253 506 562 672 938 957 1139 1831 2079
Size: 253 56 110 266 19 182 692 248
Cuts at: 2079 2781 4277 253
Size: 702 1496 376
Fragments arranged by size:
1496 702 692 376 266 253 248 182
110 56 19
BsaWI w'CCGG_w
Cuts at: 1280 1589 2110 2788 3062 3209 3620 1280
Size: 309 521 678 274 147 411 2060
Fragments arranged by size:
2060 678 521 411 309 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 205 235 604 634 205
Size: 30 369 30 3971
Fragments arranged by size:
3971 369 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 508
Size: 4400
BseYI C'CCAG_C
Cuts at: 330 1825 2583 3160 3656 330
Size: 1495 758 577 496 1074
Fragments arranged by size:
1495 1074 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 750 791 1128 1942 1988 3196 104
Size: 646 41 337 814 46 1208 1308
Fragments arranged by size:
1308 1208 814 646 337 46 41
BsiHKAI G_wGCw'C
Cuts at: 635 1022 1100 1414 2192 2382 3174 635
Size: 387 78 314 778 190 792 1861
Fragments arranged by size:
1861 792 778 387 314 190 78
Bsp1286I G_dGCh'C
Cuts at: 233 304 337 394 551 595 635 642 658
Size: 71 33 57 157 44 40 7 16
Cuts at: 658 715 735 745 978 1022 1039 1100 1271
Size: 57 20 10 233 44 17 61 171
Cuts at: 1271 1414 2025 2118 2192 2382 2444 3174 233
Size: 143 611 93 74 190 62 730 1459
Fragments arranged by size:
1459 730 611 233 190 171 157 143
93 74 71 62 61 57 57 44
44 40 33 20 17 16 10 7
BspEI T'CCGG_A
Cuts at: 1280 3620 1280
Size: 2340 2060
BspHI T'CATG_A
Cuts at: 737 3576 737
Size: 2839 1561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1109 1450 1664 1966 2347 1109
Size: 341 214 302 381 3162
Fragments arranged by size:
3162 381 341 302 214
BsrBI CCG'CTC
Cuts at: 50 156 827 2692 2746 4230 4395 50
Size: 106 671 1865 54 1484 165 55
Fragments arranged by size:
1865 1484 671 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 249 2312 249
Size: 2063 2337
BsrFI r'CCGG_y
Cuts at: 953 1075 2398 2579 2788 953
Size: 122 1323 181 209 2565
Fragments arranged by size:
2565 1323 209 181 122
BsrGI T'GTAC_A
Cuts at: 236 236
Size: 4400
BssSI C'ACGA_G
Cuts at: 600 2671 3029 600
Size: 2071 358 1971
Fragments arranged by size:
2071 1971 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1154 1777 1154
Size: 623 3777
BstBI TT'CG_AA
Cuts at: 110 2761 110
Size: 2651 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1012 1012
Size: 4400
BstYI r'GATC_y
Cuts at: 1 263 1425 1892 1915 2250 2496 3508 3594
Size: 262 1162 467 23 335 246 1012 86
Cuts at: 3594 3606 3952 1
Size: 12 346 449
Fragments arranged by size:
1162 1012 467 449 346 335 262 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1294 1294
Size: 4400
BtgI C'CryG_G
Cuts at: 305 385 893 903 305
Size: 80 508 10 3802
Fragments arranged by size:
3802 508 80 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2350 2531 2694 2350
Size: 181 163 4056
Fragments arranged by size:
4056 181 163
BtsI GCAGTG_nn'
Cuts at: 1643 1643
Size: 4400
DraI TTT'AAA
Cuts at: 1399 1399
Size: 4400
DraIII CAC_nnn'GTG
Cuts at: 894 894
Size: 4400
DrdI GACnn_nn'nnGTC
Cuts at: 2106 2964 2106
Size: 858 3542
EaeI y'GGCC_r
Cuts at: 584 747 788 1061 1077 1106 1985 2159 2550
Size: 163 41 273 16 29 879 174 391
Cuts at: 2550 2577 3922 584
Size: 27 1345 1062
Fragments arranged by size:
1345 1062 879 391 273 174 163 41
29 27 16
EagI C'GGCC_G
Cuts at: 747 788 1985 747
Size: 41 1197 3162
Fragments arranged by size:
3162 1197 41
EarI CTCTTCn'nnn_
Cuts at: 2423 2633 2423
Size: 210 4190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2608 2918 3064 4210 2608
Size: 310 146 1146 2798
Fragments arranged by size:
2798 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1412 1412
Size: 4400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 741 2225 2657 3404 3672 457
Size: 284 1484 432 747 268 1185
Fragments arranged by size:
1484 1185 747 432 284 268
EcoNI CCTnn'n_nnAGG
Cuts at: 436 436
Size: 4400
EcoO109I rG'GnC_Cy
Cuts at: 229 230 301 334 711 712 731 732 782
Size: 1 71 33 377 1 19 1 50
Cuts at: 782 809 1036 1116 1267 1539 229
Size: 27 227 80 151 272 3090
Fragments arranged by size:
3090 377 272 227 151 80 71 50
33 27 19 1 1 1
EcoRI G'AATT_C
Cuts at: 1404 1404
Size: 4400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4400
FseI GG_CCGG'CC
Cuts at: 1079 1079
Size: 4400
FspI TGC'GCA
Cuts at: 2181 2181
Size: 4400
HaeII r_GCGC'y
Cuts at: 675 960 1834 2082 3104 4083 675
Size: 285 874 248 1022 979 992
Fragments arranged by size:
1022 992 979 874 285 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 472 504 766 798 2242 2272 2274
Size: 32 235 32 262 32 1444 30 2
Cuts at: 2274 2304 205
Size: 30 2301
Fragments arranged by size:
2301 1444 262 235 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 251 1439 251
Size: 1188 3212
KasI G'GCGC_C
Cuts at: 671 956 1830 2078 671
Size: 285 874 248 2993
Fragments arranged by size:
2993 874 285 248
KpnI G_GTAC'C
Cuts at: 1420 1420
Size: 4400
MluI A'CGCG_T
Cuts at: 3833 3833
Size: 4400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 3071 3255 326
Size: 2745 184 1471
Fragments arranged by size:
2745 1471 184
MscI TGG'CCA
Cuts at: 2161 2161
Size: 4400
MslI CAynn'nnrTG
Cuts at: 1210 2389 2516 1210
Size: 1179 127 3094
Fragments arranged by size:
3094 1179 127
MspA1I CmG'CkG
Cuts at: 307 685 1511 1825 2185 3198 3443 307
Size: 378 826 314 360 1013 245 1264
Fragments arranged by size:
1264 1013 826 378 360 314 245
NaeI GCC'GGC
Cuts at: 955 1077 2581 955
Size: 122 1504 2774
Fragments arranged by size:
2774 1504 122
NarI GG'CG_CC
Cuts at: 672 957 1831 2079 672
Size: 285 874 248 2993
Fragments arranged by size:
2993 874 285 248
NgoMIV G'CCGG_C
Cuts at: 953 1075 2579 953
Size: 122 1504 2774
Fragments arranged by size:
2774 1504 122
NspI r_CATG'y
Cuts at: 144 1453 1953 2860 144
Size: 1309 500 907 1684
Fragments arranged by size:
1684 1309 907 500
PasI CC'CwG_GG
Cuts at: 522 661 716 522
Size: 139 55 4206
Fragments arranged by size:
4206 139 55
PciI A'CATG_T
Cuts at: 140 2856 140
Size: 2716 1684
PflMI CCAn_nnn'nTGG
Cuts at: 143 143
Size: 4400
PmeI GTTT'AAAC
Cuts at: 1399 1399
Size: 4400
PmlI CAC'GTG
Cuts at: 456 1032 456
Size: 576 3824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 346 378 604 636 3539 3571 346
Size: 32 226 32 2903 32 1175
Fragments arranged by size:
2903 1175 226 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 782 809 782
Size: 27 4373
PspOMI G'GGCC_C
Cuts at: 229 300 333 711 731 1035 1267 229
Size: 71 33 378 20 304 232 3362
Fragments arranged by size:
3362 378 304 232 71 33 20
PstI C_TGCA'G
Cuts at: 1193 1447 2132 1193
Size: 254 685 3461
Fragments arranged by size:
3461 685 254
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4400
PvuII CAG'CTG
Cuts at: 685 1825 2185 685
Size: 1140 360 2900
Fragments arranged by size:
2900 1140 360
RsrII CG'GwC_CG
Cuts at: 2595 2595
Size: 4400
SacI G_AGCT'C
Cuts at: 1414 1414
Size: 4400
SacII CC_GC'GG
Cuts at: 308 308
Size: 4400
SalI G'TCGA_C
Cuts at: 1437 1437
Size: 4400
SanDI GG'GwC_CC
Cuts at: 809 809
Size: 4400
SapI GCTCTTCn'nnn_
Cuts at: 2423 2633 2423
Size: 210 4190
SbfI CC_TGCA'GG
Cuts at: 1447 1447
Size: 4400
ScaI AGT'ACT
Cuts at: 3917 3917
Size: 4400
SfcI C'TryA_G
Cuts at: 33 1189 1443 2128 3121 3312 33
Size: 1156 254 685 993 191 1121
Fragments arranged by size:
1156 1121 993 685 254 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 367 1081 367
Size: 714 3686
SfoI GGC'GCC
Cuts at: 673 958 1832 2080 673
Size: 285 874 248 2993
Fragments arranged by size:
2993 874 285 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
SmaI CCC'GGG
Cuts at: 190 948 1422 190
Size: 758 474 3168
Fragments arranged by size:
3168 758 474
SmlI C'TyrA_G
Cuts at: 1327 1555 2470 2962 3224 1327
Size: 228 915 492 262 2503
Fragments arranged by size:
2503 915 492 262 228
SnaBI TAC'GTA
Cuts at: 1392 1392
Size: 4400
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4400
SphI G_CATG'C
Cuts at: 1453 1953 1453
Size: 500 3900
StuI AGG'CCT
Cuts at: 220 220
Size: 4400
StyI C'CwwG_G
Cuts at: 224 551 689 869 1534 1888 224
Size: 327 138 180 665 354 2736
Fragments arranged by size:
2736 665 354 327 180 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1983 2116 2575 2784 1983
Size: 133 459 209 3599
Fragments arranged by size:
3599 459 209 133
TatI w'GTAC_w
Cuts at: 236 3915 236
Size: 3679 721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3917 3949 3917
Size: 32 4368
Tth111I GACn'n_nGTC
Cuts at: 2197 2197
Size: 4400
XbaI T'CTAG_A
Cuts at: 1431 1431
Size: 4400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1895 1895
Size: 4400
XmaI C'CCGG_G
Cuts at: 188 946 1420 188
Size: 758 474 3168
Fragments arranged by size:
3168 758 474
ZraI GAC'GTC
Cuts at: 563 563
Size: 4400
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AscI AvrII BaeI BbvCI BfrBI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PshAI PsiI PspXI PsrI SexAI SgrAI SrfI SspI
SwaI XhoI XmnI