Restriction map for
pF1KSDA1959
(Circular) (Six-base) MAPSORT of: pF1KSDA1959.seq Check: 1167 from: 1 to: 5084
pF1KSDA1959 5084 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 833 833
Size: 5084
AccI GT'mk_AC
Cuts at: 2122 2122
Size: 5084
Acc65I G'GTAC_C
Cuts at: 2100 2100
Size: 5084
AclI AA'CG_TT
Cuts at: 4924 4924
Size: 5084
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1870 2909 3341 4088 4356 1870
Size: 1039 432 747 268 2598
Fragments arranged by size:
2598 1039 747 432 268
AflIII A'CryG_T
Cuts at: 759 2436 3540 4517 759
Size: 1677 1104 977 1326
Fragments arranged by size:
1677 1326 1104 977
AgeI A'CCGG_T
Cuts at: 3472 3472
Size: 5084
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 305 337 305
Size: 32 5052
AlwNI CAG_nnn'CTG
Cuts at: 584 712 1091 1242 1893 2389 3956 584
Size: 128 379 151 651 496 1567 1712
Fragments arranged by size:
1712 1567 651 496 379 151 128
ApaLI G'TGCA_C
Cuts at: 3854 3854
Size: 5084
ApoI r'AATT_y
Cuts at: 2088 4437 4693 2088
Size: 2349 256 2479
Fragments arranged by size:
2479 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5084
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
AvaI C'yCGr_G
Cuts at: 386 869 955 1195 2104 386
Size: 483 86 240 909 3366
Fragments arranged by size:
3366 909 483 240 86
BamHI G'GATC_C
Cuts at: 2109 2109
Size: 5084
BanI G'GyrC_C
Cuts at: 223 2100 2514 2762 2797 223
Size: 1877 414 248 35 2510
Fragments arranged by size:
2510 1877 414 248 35
BanII G_rGCy'C
Cuts at: 282 623 1196 1616 2098 3128 282
Size: 341 573 420 482 1030 2238
Fragments arranged by size:
2238 1030 573 482 420 341
BbeI G_GCGC'C
Cuts at: 2518 2766 2518
Size: 248 4836
BbsI GAAGACnn'nnnn_
Cuts at: 668 668
Size: 5084
BbvCI CC'TCA_GC
Cuts at: 1243 1403 1243
Size: 160 4924
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1420 1454 1420
Size: 34 5050
BciVI GTATCCnnnnn_n'
Cuts at: 1661 2982 3743 5084 1661
Size: 1321 761 1341 1661
Fragments arranged by size:
1661 1341 1321 761
BclI T'GATC_A
Cuts at: 2604 2604
Size: 5084
BfrBI ATG'CAT
Cuts at: 1463 1463
Size: 5084
BglI GCCn_nnn'nGGC
Cuts at: 1262 1262
Size: 5084
BglII A'GATC_T
Cuts at: 464 2599 4636 464
Size: 2135 2037 912
Fragments arranged by size:
2135 2037 912
BlpI GC'TnA_GC
Cuts at: 2196 2196
Size: 5084
Bme1580I G_kGCm'C
Cuts at: 2709 2802 3858 2709
Size: 93 1056 3935
Fragments arranged by size:
3935 1056 93
BmgBI CAC'GTC
Cuts at: 252 1022 252
Size: 770 4314
BmrI ACTGGGnnnn_n'
Cuts at: 140 858 1335 2045 2296 2377 2712 4856 140
Size: 718 477 710 251 81 335 2144 368
Fragments arranged by size:
2144 718 710 477 368 335 251 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3451 3451
Size: 5084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 964 2070 637
Size: 327 1106 3651
Fragments arranged by size:
3651 1106 327
Bpu10I CC'TnA_GC
Cuts at: 423 1243 1403 423
Size: 820 160 4104
Fragments arranged by size:
4104 820 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 2260 3139 3631 3929 664
Size: 1596 879 492 298 1819
Fragments arranged by size:
1819 1596 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2050 4788 2050
Size: 2738 2346
BsaAI yAC'GTr
Cuts at: 2076 2437 2076
Size: 361 4723
BsaBI GATnn'nnATC
Cuts at: 6 2598 4635 6
Size: 2592 2037 455
Fragments arranged by size:
2592 2037 455
BsaHI Gr'CG_yC
Cuts at: 830 2515 2763 3465 4961 830
Size: 1685 248 702 1496 953
Fragments arranged by size:
1685 1496 953 702 248
BsaWI w'CCGG_w
Cuts at: 2273 2794 3472 3746 3893 4304 2273
Size: 521 678 274 147 411 3053
Fragments arranged by size:
3053 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1401 1431 1401
Size: 30 5054
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 247 1199 187
Size: 60 952 4072
Fragments arranged by size:
4072 952 60
BseYI C'CCAG_C
Cuts at: 1645 2509 3267 3844 4340 1645
Size: 864 758 577 496 2389
Fragments arranged by size:
2389 864 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1541
Size: 5084
BsiEI CG_ry'CG
Cuts at: 104 2626 2672 3880 104
Size: 2522 46 1208 1308
Fragments arranged by size:
2522 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 257 623 1654 2098 2876 3066 3858 257
Size: 366 1031 444 778 190 792 1483
Fragments arranged by size:
1483 1031 792 778 444 366 190
BsmI GAATG_Cn'
Cuts at: 1087 1087
Size: 5084
BsmBI CGTCTCn'nnnn_
Cuts at: 1195 1195
Size: 5084
Bsp1286I G_dGCh'C
Cuts at: 257 282 623 1196 1616 1654 2098 2709 2802
Size: 25 341 573 420 38 444 611 93
Cuts at: 2802 2876 3066 3128 3858 257
Size: 74 190 62 730 1483
Fragments arranged by size:
1483 730 611 573 444 420 341 190
93 74 62 38 25
BspEI T'CCGG_A
Cuts at: 4304 4304
Size: 5084
BspHI T'CATG_A
Cuts at: 4260 4260
Size: 5084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1668 1885 2134 2348 2650 3031 1668
Size: 217 249 214 302 381 3721
Fragments arranged by size:
3721 381 302 249 217 214
BsrBI CCG'CTC
Cuts at: 50 153 3376 3430 4914 5079 50
Size: 103 3223 54 1484 165 55
Fragments arranged by size:
3223 1484 165 103 55 54
BsrDI GCAATG_nn'
Cuts at: 2996 2996
Size: 5084
BsrFI r'CCGG_y
Cuts at: 415 1033 3082 3263 3472 415
Size: 618 2049 181 209 2027
Fragments arranged by size:
2049 2027 618 209 181
BsrGI T'GTAC_A
Cuts at: 179 179
Size: 5084
BssSI C'ACGA_G
Cuts at: 3355 3713 3355
Size: 358 4726
BstAPI GCAn_nnn'nTGC
Cuts at: 712 2461 712
Size: 1749 3335
BstBI TT'CG_AA
Cuts at: 110 3445 110
Size: 3335 1749
BstEII G'GTnAC_C
Cuts at: 358 358
Size: 5084
BstYI r'GATC_y
Cuts at: 1 464 1010 1601 1937 2001 2109 2576 2599
Size: 463 546 591 336 64 108 467 23
Cuts at: 2599 2934 3180 4192 4278 4290 4636 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 591 546 467 463 449 346 336
335 246 108 86 64 23 12
BtgI C'CryG_G
Cuts at: 125 263 305 572 2025 125
Size: 138 42 267 1453 3184
Fragments arranged by size:
3184 1453 267 138 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1290 3034 3215 3378 1290
Size: 1744 181 163 2996
Fragments arranged by size:
2996 1744 181 163
BtsI GCAGTG_nn'
Cuts at: 2327 2327
Size: 5084
ClaI AT'CG_AT
Cuts at: 1514 1514
Size: 5084
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1460 1495 1460
Size: 35 5049
DraI TTT'AAA
Cuts at: 2083 2083
Size: 5084
DrdI GACnn_nn'nnGTC
Cuts at: 2790 3648 2790
Size: 858 4226
EaeI y'GGCC_r
Cuts at: 138 2669 2843 3234 3261 4606 138
Size: 2531 174 391 27 1345 616
Fragments arranged by size:
2531 1345 616 391 174 27
EagI C'GGCC_G
Cuts at: 2669 2669
Size: 5084
EarI CTCTTCn'nnn_
Cuts at: 3107 3317 3107
Size: 210 4874
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3292 3602 3748 4894 3292
Size: 310 146 1146 3482
Fragments arranged by size:
3482 1146 310 146
EcoICRI GAG'CTC
Cuts at: 621 2096 621
Size: 1475 3609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 964 1870 2070 2909 3341 4088 4356 637
Size: 327 906 200 839 432 747 268 1365
Fragments arranged by size:
1365 906 839 747 432 327 268 200
EcoO109I rG'GnC_Cy
Cuts at: 675 1890 2223 675
Size: 1215 333 3536
Fragments arranged by size:
3536 1215 333
EcoRI G'AATT_C
Cuts at: 2088 2088
Size: 5084
EcoRV GAT'ATC
Cuts at: 876 876
Size: 5084
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 5084
FspI TGC'GCA
Cuts at: 2865 2865
Size: 5084
HaeII r_GCGC'y
Cuts at: 219 247 2518 2766 3788 4767 219
Size: 28 2271 248 1022 979 536
Fragments arranged by size:
2271 1022 979 536 248 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 700 732 1194 1226 1929 1961 2926 2956 2958
Size: 32 462 32 703 32 965 30 2
Cuts at: 2958 2988 700
Size: 30 2796
Fragments arranged by size:
2796 965 703 462 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1251 2123 1251
Size: 872 4212
HindIII A'AGCT_T
Cuts at: 433 433
Size: 5084
KasI G'GCGC_C
Cuts at: 2514 2762 2514
Size: 248 4836
KpnI G_GTAC'C
Cuts at: 2104 2104
Size: 5084
MluI A'CGCG_T
Cuts at: 4517 4517
Size: 5084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 464 659 988 1160 3755 3939 464
Size: 195 329 172 2595 184 1609
Fragments arranged by size:
2595 1609 329 195 184 172
MscI TGG'CCA
Cuts at: 2845 2845
Size: 5084
MslI CAynn'nnrTG
Cuts at: 356 970 1450 3073 3200 356
Size: 614 480 1623 127 2240
Fragments arranged by size:
2240 1623 614 480 127
MspA1I CmG'CkG
Cuts at: 609 1242 1407 1995 2195 2509 2869 3882 4127
Size: 633 165 588 200 314 360 1013 245
Cuts at: 4127 609
Size: 1566
Fragments arranged by size:
1566 1013 633 588 360 314 245 200
165
NaeI GCC'GGC
Cuts at: 3265 3265
Size: 5084
NarI GG'CG_CC
Cuts at: 2515 2763 2515
Size: 248 4836
NcoI C'CATG_G
Cuts at: 125 263 2025 125
Size: 138 1762 3184
Fragments arranged by size:
3184 1762 138
NgoMIV G'CCGG_C
Cuts at: 3263 3263
Size: 5084
NsiI A_TGCA'T
Cuts at: 1465 1465
Size: 5084
NspI r_CATG'y
Cuts at: 335 736 763 1389 1467 2137 2637 3544 335
Size: 401 27 626 78 670 500 907 1875
Fragments arranged by size:
1875 907 670 626 500 401 78 27
PasI CC'CwG_GG
Cuts at: 552 552
Size: 5084
PciI A'CATG_T
Cuts at: 759 3540 759
Size: 2781 2303
PfoI T'CCnGG_A
Cuts at: 368 368
Size: 5084
PmeI GTTT'AAAC
Cuts at: 2083 2083
Size: 5084
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 4223 4255 114
Size: 32 4077 32 943
Fragments arranged by size:
4077 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 675 675
Size: 5084
PshAI GACnn'nnGTC
Cuts at: 574 574
Size: 5084
PstI C_TGCA'G
Cuts at: 569 708 1692 2131 2816 569
Size: 139 984 439 685 2837
Fragments arranged by size:
2837 984 685 439 139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5084
PvuII CAG'CTG
Cuts at: 1995 2509 2869 1995
Size: 514 360 4210
Fragments arranged by size:
4210 514 360
RsrII CG'GwC_CG
Cuts at: 3279 3279
Size: 5084
SacI G_AGCT'C
Cuts at: 623 2098 623
Size: 1475 3609
SalI G'TCGA_C
Cuts at: 2121 2121
Size: 5084
SapI GCTCTTCn'nnn_
Cuts at: 3107 3317 3107
Size: 210 4874
SbfI CC_TGCA'GG
Cuts at: 2131 2131
Size: 5084
ScaI AGT'ACT
Cuts at: 1330 4601 1330
Size: 3271 1813
SfcI C'TryA_G
Cuts at: 33 565 704 1688 1716 2127 2812 3805 3996
Size: 532 139 984 28 411 685 993 191
Cuts at: 3996 33
Size: 1121
Fragments arranged by size:
1121 993 984 685 532 411 191 139
28
SfoI GGC'GCC
Cuts at: 2516 2764 2516
Size: 248 4836
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
SmaI CCC'GGG
Cuts at: 388 957 2106 388
Size: 569 1149 3366
Fragments arranged by size:
3366 1149 569
SmlI C'TyrA_G
Cuts at: 679 2239 3154 3646 3908 679
Size: 1560 915 492 262 1855
Fragments arranged by size:
1855 1560 915 492 262
SnaBI TAC'GTA
Cuts at: 2076 2076
Size: 5084
SpeI A'CTAG_T
Cuts at: 67 1476 67
Size: 1409 3675
SphI G_CATG'C
Cuts at: 1467 2137 2637 1467
Size: 670 500 3914
Fragments arranged by size:
3914 670 500
StyI C'CwwG_G
Cuts at: 125 263 296 1070 1910 2025 2218 2572 125
Size: 138 33 774 840 115 193 354 2637
Fragments arranged by size:
2637 840 774 354 193 138 115 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 840 1471 2667 2800 3259 3468 840
Size: 631 1196 133 459 209 2456
Fragments arranged by size:
2456 1196 631 459 209 133
TatI w'GTAC_w
Cuts at: 179 1328 4599 179
Size: 1149 3271 664
Fragments arranged by size:
3271 1149 664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1843 1875 4601 4633 1843
Size: 32 2726 32 2294
Fragments arranged by size:
2726 2294 32 32
Tth111I GACn'n_nGTC
Cuts at: 2881 2881
Size: 5084
XbaI T'CTAG_A
Cuts at: 2115 2115
Size: 5084
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2032 2579 2032
Size: 547 4537
XmaI C'CCGG_G
Cuts at: 386 955 2104 386
Size: 569 1149 3366
Fragments arranged by size:
3366 1149 569
ZraI GAC'GTC
Cuts at: 831 831
Size: 5084
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BmtI BsiWI BssHII BstXI BstZ17I Bsu36I
DraIII EcoNI FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PflMI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI