Restriction map for
pF1KSDA1963
(Circular) (Six-base) MAPSORT of: pF1KSDA1963.seq Check: 4350 from: 1 to: 4108
pF1KSDA1963 4108 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 551 551
Size: 4108
AccI GT'mk_AC
Cuts at: 1094 1144 1094
Size: 50 4058
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4108
AclI AA'CG_TT
Cuts at: 3948 3948
Size: 4108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1931 2363 3112 3380 1931
Size: 432 749 268 2659
Fragments arranged by size:
2659 749 432 268
AflIII A'CryG_T
Cuts at: 434 1458 2564 3541 434
Size: 1024 1106 977 1001
Fragments arranged by size:
1106 1024 1001 977
AgeI A'CCGG_T
Cuts at: 2496 2496
Size: 4108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 232 559 1017 232
Size: 327 458 3323
Fragments arranged by size:
3323 458 327
AlwNI CAG_nnn'CTG
Cuts at: 1411 2980 1411
Size: 1569 2539
ApaLI G'TGCA_C
Cuts at: 2878 2878
Size: 4108
ApoI r'AATT_y
Cuts at: 1110 3461 3717 1110
Size: 2351 256 1501
Fragments arranged by size:
2351 1501 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4108
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4108
AvaI C'yCGr_G
Cuts at: 577 1126 577
Size: 549 3559
BamHI G'GATC_C
Cuts at: 170 464 922 1131 170
Size: 294 458 209 3147
Fragments arranged by size:
3147 458 294 209
BanI G'GyrC_C
Cuts at: 271 635 1122 1536 1784 1819 271
Size: 364 487 414 248 35 2560
Fragments arranged by size:
2560 487 414 364 248 35
BanII G_rGCy'C
Cuts at: 707 1120 2150 707
Size: 413 1030 2665
Fragments arranged by size:
2665 1030 413
BbeI G_GCGC'C
Cuts at: 275 1540 1788 275
Size: 1265 248 2595
Fragments arranged by size:
2595 1265 248
BbsI GAAGACnn'nnnn_
Cuts at: 868 868
Size: 4108
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2767 4108 2004
Size: 763 1341 2004
Fragments arranged by size:
2004 1341 763
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4108
BglI GCCn_nnn'nGGC
Cuts at: 269 269
Size: 4108
BglII A'GATC_T
Cuts at: 1621 3660 1621
Size: 2039 2069
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4108
Bme1580I G_kGCm'C
Cuts at: 221 554 1731 1824 2882 221
Size: 333 1177 93 1058 1447
Fragments arranged by size:
1447 1177 1058 333 93
BmgBI CAC'GTC
Cuts at: 198 198
Size: 4108
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1399 1734 3880 1318
Size: 81 335 2146 1546
Fragments arranged by size:
2146 1546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 846 721
Size: 125 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2161 2655 2953 1282
Size: 879 494 298 2437
Fragments arranged by size:
2437 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 741 3812 741
Size: 3071 1037
BsaAI yAC'GTr
Cuts at: 549 1098 1459 549
Size: 549 361 3198
Fragments arranged by size:
3198 549 361
BsaBI GATnn'nnATC
Cuts at: 6 1620 3659 6
Size: 1614 2039 455
Fragments arranged by size:
2039 1614 455
BsaHI Gr'CG_yC
Cuts at: 272 1537 1785 2489 3985 272
Size: 1265 248 704 1496 395
Fragments arranged by size:
1496 1265 704 395 248
BsaWI w'CCGG_w
Cuts at: 1295 1816 2496 2770 2917 3328 1295
Size: 521 680 274 147 411 2075
Fragments arranged by size:
2075 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 269 299 269
Size: 30 4078
BseYI C'CCAG_C
Cuts at: 532 926 1531 2289 2868 3364 532
Size: 394 605 758 579 496 1276
Fragments arranged by size:
1276 758 605 579 496 394
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 442 529 987 424
Size: 18 87 458 3545
Fragments arranged by size:
3545 458 87 18
BsiEI CG_ry'CG
Cuts at: 104 337 1648 1694 2904 104
Size: 233 1311 46 1210 1308
Fragments arranged by size:
1311 1308 1210 233 46
BsiHKAI G_wGCw'C
Cuts at: 668 707 1120 1898 2088 2882 668
Size: 39 413 778 190 794 1894
Fragments arranged by size:
1894 794 778 413 190 39
Bsp1286I G_dGCh'C
Cuts at: 221 554 668 707 1120 1731 1824 1898 2088
Size: 333 114 39 413 611 93 74 190
Cuts at: 2088 2150 2882 221
Size: 62 732 1447
Fragments arranged by size:
1447 732 611 413 333 190 114 93
74 62 39
BspEI T'CCGG_A
Cuts at: 3328 3328
Size: 4108
BspHI T'CATG_A
Cuts at: 3284 3284
Size: 4108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 551 1156 1370 1672 2053 551
Size: 605 214 302 381 2606
Fragments arranged by size:
2606 605 381 302 214
BsrBI CCG'CTC
Cuts at: 50 225 285 2398 2452 3938 4103 50
Size: 175 60 2113 54 1486 165 55
Fragments arranged by size:
2113 1486 175 165 60 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4108
BsrFI r'CCGG_y
Cuts at: 820 2104 2285 2496 820
Size: 1284 181 211 2432
Fragments arranged by size:
2432 1284 211 181
BssHII G'CGCG_C
Cuts at: 487 588 648 650 487
Size: 101 60 2 3945
Fragments arranged by size:
3945 101 60 2
BssSI C'ACGA_G
Cuts at: 1015 2377 2737 1015
Size: 1362 360 2386
Fragments arranged by size:
2386 1362 360
BstAPI GCAn_nnn'nTGC
Cuts at: 865 1483 865
Size: 618 3490
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4108
BstXI CCAn_nnnn'nTGG
Cuts at: 855 855
Size: 4108
BstYI r'GATC_y
Cuts at: 1 170 464 922 1131 1598 1621 1956 2202
Size: 169 294 458 209 467 23 335 246
Cuts at: 2202 3216 3302 3314 3660 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 467 458 449 346 335 294 246
209 169 86 23 12
BstZ17I GTA'TAC
Cuts at: 1095 1095
Size: 4108
BtgI C'CryG_G
Cuts at: 264 559 264
Size: 295 3813
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3764
Fragments arranged by size:
3764 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1349
Size: 4108
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4108
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2672 1812
Size: 860 3248
EaeI y'GGCC_r
Cuts at: 334 428 1691 1865 2256 2283 3630 334
Size: 94 1263 174 391 27 1347 812
Fragments arranged by size:
1347 1263 812 391 174 94 27
EagI C'GGCC_G
Cuts at: 334 1691 334
Size: 1357 2751
EarI CTCTTCn'nnn_
Cuts at: 673 712 2129 2339 673
Size: 39 1417 210 2442
Fragments arranged by size:
2442 1417 210 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 430 2314 2626 2772 3918 430
Size: 1884 312 146 1146 620
Fragments arranged by size:
1884 1146 620 312 146
EcoICRI GAG'CTC
Cuts at: 705 1118 705
Size: 413 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 846 1931 2363 3112 3380 721
Size: 125 1085 432 749 268 1449
Fragments arranged by size:
1449 1085 749 432 268 125
EcoNI CCTnn'n_nnAGG
Cuts at: 472 472
Size: 4108
EcoO109I rG'GnC_Cy
Cuts at: 311 1245 311
Size: 934 3174
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 4108
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4108
HaeII r_GCGC'y
Cuts at: 275 567 774 1540 1788 2812 3791 275
Size: 292 207 766 248 1024 979 592
Fragments arranged by size:
1024 979 766 592 292 248 207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 269 301 1948 1978 1980 2010 269
Size: 32 1647 30 2 30 2367
Fragments arranged by size:
2367 1647 32 30 30 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4108
KasI G'GCGC_C
Cuts at: 271 1536 1784 271
Size: 1265 248 2595
Fragments arranged by size:
2595 1265 248
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4108
MluI A'CGCG_T
Cuts at: 3541 3541
Size: 4108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2779 2963 2779
Size: 184 3924
MscI TGG'CCA
Cuts at: 430 1867 430
Size: 1437 2671
MslI CAynn'nnrTG
Cuts at: 187 853 2095 2222 187
Size: 666 1242 127 2073
Fragments arranged by size:
2073 1242 666 127
MspA1I CmG'CkG
Cuts at: 266 360 456 561 786 1217 1531 1891 2906
Size: 94 96 105 225 431 314 360 1015
Cuts at: 2906 3151 266
Size: 245 1223
Fragments arranged by size:
1223 1015 431 360 314 245 225 105
96 94
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4108
NarI GG'CG_CC
Cuts at: 272 1537 1785 272
Size: 1265 248 2595
Fragments arranged by size:
2595 1265 248
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4108
NruI TCG'CGA
Cuts at: 2469 2469
Size: 4108
NspI r_CATG'y
Cuts at: 1159 1659 2568 1159
Size: 500 909 2699
Fragments arranged by size:
2699 909 500
PciI A'CATG_T
Cuts at: 2564 2564
Size: 4108
PfoI T'CCnGG_A
Cuts at: 710 710
Size: 4108
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4108
PmlI CAC'GTG
Cuts at: 549 549
Size: 4108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3247 3279 3247
Size: 32 4076
PpuMI rG'GwC_Cy
Cuts at: 311 311
Size: 4108
PstI C_TGCA'G
Cuts at: 1153 1838 1153
Size: 685 3423
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4108
PvuII CAG'CTG
Cuts at: 360 456 1531 1891 360
Size: 96 1075 360 2577
Fragments arranged by size:
2577 1075 360 96
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4108
SacI G_AGCT'C
Cuts at: 707 1120 707
Size: 413 3695
SacII CC_GC'GG
Cuts at: 267 562 267
Size: 295 3813
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4108
SanDI GG'GwC_CC
Cuts at: 311 311
Size: 4108
SapI GCTCTTCn'nnn_
Cuts at: 673 712 2129 2339 673
Size: 39 1417 210 2442
Fragments arranged by size:
2442 1417 210 39
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4108
ScaI AGT'ACT
Cuts at: 3625 3625
Size: 4108
SfcI C'TryA_G
Cuts at: 33 390 693 1149 1834 2829 3020 33
Size: 357 303 456 685 995 191 1121
Fragments arranged by size:
1121 995 685 456 357 303 191
SfoI GGC'GCC
Cuts at: 273 1538 1786 273
Size: 1265 248 2595
Fragments arranged by size:
2595 1265 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4108
SmaI CCC'GGG
Cuts at: 579 1128 579
Size: 549 3559
SmlI C'TyrA_G
Cuts at: 1261 2176 2670 2932 1261
Size: 915 494 262 2437
Fragments arranged by size:
2437 915 494 262
SnaBI TAC'GTA
Cuts at: 1098 1098
Size: 4108
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4108
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3608
SrfI GCCC'GGGC
Cuts at: 579 579
Size: 4108
StuI AGG'CCT
Cuts at: 840 840
Size: 4108
StyI C'CwwG_G
Cuts at: 1240 1594 1240
Size: 354 3754
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1689 1822 2281 2492 1689
Size: 133 459 211 3305
Fragments arranged by size:
3305 459 211 133
TatI w'GTAC_w
Cuts at: 3623 3623
Size: 4108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3625 3657 3625
Size: 32 4076
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4108
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1601 1601
Size: 4108
XmaI C'CCGG_G
Cuts at: 577 1126 577
Size: 549 3559
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BseRI
BsiWI BsmI BsmBI BsrGI BstEII Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NsiI PacI PasI PflMI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SspI
SwaI XhoI XmnI ZraI