Restriction map for
pF1KSDA1969
(Circular) (Six-base) MAPSORT of: pF1KSDA1969.seq Check: 841 from: 1 to: 4867
pF1KSDA1969 4867 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1903 1903
Size: 4867
Acc65I G'GTAC_C
Cuts at: 1881 1881
Size: 4867
AclI AA'CG_TT
Cuts at: 4707 4707
Size: 4867
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 3122 3871 4139 2690
Size: 432 749 268 3418
Fragments arranged by size:
3418 749 432 268
AflIII A'CryG_T
Cuts at: 2217 3323 4300 2217
Size: 1106 977 2784
Fragments arranged by size:
2784 1106 977
AgeI A'CCGG_T
Cuts at: 1019 1439 3255 1019
Size: 420 1816 2631
Fragments arranged by size:
2631 1816 420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 189 189
Size: 4867
AlwNI CAG_nnn'CTG
Cuts at: 2170 3739 2170
Size: 1569 3298
ApaLI G'TGCA_C
Cuts at: 592 3637 592
Size: 3045 1822
ApoI r'AATT_y
Cuts at: 253 297 546 685 797 813 881 1301 1553
Size: 44 249 139 112 16 68 420 252
Cuts at: 1553 1721 1869 4220 4476 253
Size: 168 148 2351 256 644
Fragments arranged by size:
2351 644 420 256 252 249 168 148
139 112 68 44 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 4867
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4867
AvaI C'yCGr_G
Cuts at: 1885 1885
Size: 4867
BamHI G'GATC_C
Cuts at: 1890 1890
Size: 4867
BanI G'GyrC_C
Cuts at: 1881 2295 2543 2578 1881
Size: 414 248 35 4170
Fragments arranged by size:
4170 414 248 35
BanII G_rGCy'C
Cuts at: 404 1879 2909 404
Size: 1475 1030 2362
Fragments arranged by size:
2362 1475 1030
BbeI G_GCGC'C
Cuts at: 2299 2547 2299
Size: 248 4619
BbsI GAAGACnn'nnnn_
Cuts at: 333 1100 333
Size: 767 4100
BciVI GTATCCnnnnn_n'
Cuts at: 161 2763 3526 4867 161
Size: 2602 763 1341 161
Fragments arranged by size:
2602 1341 763 161
BclI T'GATC_A
Cuts at: 2385 2385
Size: 4867
BglII A'GATC_T
Cuts at: 2380 4419 2380
Size: 2039 2828
BlpI GC'TnA_GC
Cuts at: 290 1977 290
Size: 1687 3180
Bme1580I G_kGCm'C
Cuts at: 596 2490 2583 3641 596
Size: 1894 93 1058 1822
Fragments arranged by size:
1894 1822 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 367 983 2077 2158 2493 4639 367
Size: 616 1094 81 335 2146 595
Fragments arranged by size:
2146 1094 616 595 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1838 3232 1838
Size: 1394 3473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 406 911 1079 1163 1247 1331 1415 1499
Size: 123 505 168 84 84 84 84 84
Cuts at: 1499 1583 1833 283
Size: 84 250 3317
Fragments arranged by size:
3317 505 250 168 123 84 84 84
84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 2041 2920 3414 3712 142
Size: 1899 879 494 298 1297
Fragments arranged by size:
1899 1297 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4571 4571
Size: 4867
BsaAI yAC'GTr
Cuts at: 1857 2218 1857
Size: 361 4506
BsaBI GATnn'nnATC
Cuts at: 6 2379 4418 6
Size: 2373 2039 455
Fragments arranged by size:
2373 2039 455
BsaHI Gr'CG_yC
Cuts at: 2296 2544 3248 4744 2296
Size: 248 704 1496 2419
Fragments arranged by size:
2419 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1019 1439 2054 2575 3255 3529 3676 4087 1019
Size: 420 615 521 680 274 147 411 1799
Fragments arranged by size:
1799 680 615 521 420 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 280
Size: 4867
BseYI C'CCAG_C
Cuts at: 441 2290 3048 3627 4123 441
Size: 1849 758 579 496 1185
Fragments arranged by size:
1849 1185 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1442 1442
Size: 4867
BsiEI CG_ry'CG
Cuts at: 104 2407 2453 3663 104
Size: 2303 46 1210 1308
Fragments arranged by size:
2303 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 596 1879 2657 2847 3641 596
Size: 1283 778 190 794 1822
Fragments arranged by size:
1822 1283 794 778 190
BsmI GAATG_Cn'
Cuts at: 280 280
Size: 4867
Bsp1286I G_dGCh'C
Cuts at: 404 596 1879 2490 2583 2657 2847 2909 3641
Size: 192 1283 611 93 74 190 62 732
Cuts at: 3641 404
Size: 1630
Fragments arranged by size:
1630 1283 732 611 192 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4087 4087
Size: 4867
BspHI T'CATG_A
Cuts at: 4043 4043
Size: 4867
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1915 2129 2431 2812 1915
Size: 214 302 381 3970
Fragments arranged by size:
3970 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3157 3211 4697 4862 50
Size: 3107 54 1486 165 55
Fragments arranged by size:
3107 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2777 2777
Size: 4867
BsrFI r'CCGG_y
Cuts at: 1019 1439 2863 3044 3255 1019
Size: 420 1424 181 211 2631
Fragments arranged by size:
2631 1424 420 211 181
BssSI C'ACGA_G
Cuts at: 3136 3496 3136
Size: 360 4507
BstAPI GCAn_nnn'nTGC
Cuts at: 2242 2242
Size: 4867
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4867
BstYI r'GATC_y
Cuts at: 1 1890 2357 2380 2715 2961 3975 4061 4073
Size: 1889 467 23 335 246 1014 86 12
Cuts at: 4073 4419 1
Size: 346 449
Fragments arranged by size:
1889 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4867
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2815 2996 3159 2815
Size: 181 163 4523
Fragments arranged by size:
4523 181 163
BtsI GCAGTG_nn'
Cuts at: 2108 2108
Size: 4867
ClaI AT'CG_AT
Cuts at: 1188 1188
Size: 4867
DraI TTT'AAA
Cuts at: 692 849 1864 692
Size: 157 1015 3695
Fragments arranged by size:
3695 1015 157
DrdI GACnn_nn'nnGTC
Cuts at: 2571 3431 2571
Size: 860 4007
EaeI y'GGCC_r
Cuts at: 245 475 2450 2624 3015 3042 4389 245
Size: 230 1975 174 391 27 1347 723
Fragments arranged by size:
1975 1347 723 391 230 174 27
EagI C'GGCC_G
Cuts at: 2450 2450
Size: 4867
EarI CTCTTCn'nnn_
Cuts at: 409 2888 3098 409
Size: 2479 210 2178
Fragments arranged by size:
2479 2178 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3073 3385 3531 4677 3073
Size: 312 146 1146 3263
Fragments arranged by size:
3263 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1877 1877
Size: 4867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 406 911 1079 1163 1247 1331 1415 1499
Size: 123 505 168 84 84 84 84 84
Cuts at: 1499 1583 1833 2690 3122 3871 4139 283
Size: 84 250 857 432 749 268 1011
Fragments arranged by size:
1011 857 749 505 432 268 250 168
123 84 84 84 84 84 84
EcoO109I rG'GnC_Cy
Cuts at: 2004 2004
Size: 4867
EcoRI G'AATT_C
Cuts at: 253 797 813 881 1301 1553 1869 253
Size: 544 16 68 420 252 316 3251
Fragments arranged by size:
3251 544 420 316 252 68 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 4867
FspI TGC'GCA
Cuts at: 2646 2646
Size: 4867
HaeII r_GCGC'y
Cuts at: 2299 2547 3571 4550 2299
Size: 248 1024 979 2616
Fragments arranged by size:
2616 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2707 2737 2739 2769 2707
Size: 30 2 30 4805
Fragments arranged by size:
4805 30 30 2
HincII GTy'rAC
Cuts at: 787 1904 787
Size: 1117 3750
HindIII A'AGCT_T
Cuts at: 842 926 1094 1178 1430 1514 1598 842
Size: 84 168 84 252 84 84 4111
Fragments arranged by size:
4111 252 168 84 84 84 84
KasI G'GCGC_C
Cuts at: 2295 2543 2295
Size: 248 4619
KpnI G_GTAC'C
Cuts at: 1885 1885
Size: 4867
MluI A'CGCG_T
Cuts at: 4300 4300
Size: 4867
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3538 3722 3538
Size: 184 4683
MscI TGG'CCA
Cuts at: 247 477 2626 247
Size: 230 2149 2488
Fragments arranged by size:
2488 2149 230
MslI CAynn'nnrTG
Cuts at: 990 2854 2981 990
Size: 1864 127 2876
Fragments arranged by size:
2876 1864 127
MspA1I CmG'CkG
Cuts at: 1976 2290 2650 3665 3910 1976
Size: 314 360 1015 245 2933
Fragments arranged by size:
2933 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3046 3046
Size: 4867
NarI GG'CG_CC
Cuts at: 2296 2544 2296
Size: 248 4619
NcoI C'CATG_G
Cuts at: 125 125
Size: 4867
NgoMIV G'CCGG_C
Cuts at: 3044 3044
Size: 4867
NruI TCG'CGA
Cuts at: 3228 3228
Size: 4867
NspI r_CATG'y
Cuts at: 771 1918 2418 3327 771
Size: 1147 500 909 2311
Fragments arranged by size:
2311 1147 909 500
PasI CC'CwG_GG
Cuts at: 178 178
Size: 4867
PciI A'CATG_T
Cuts at: 3323 3323
Size: 4867
PmeI GTTT'AAAC
Cuts at: 1864 1864
Size: 4867
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4006 4038 4006
Size: 32 4835
PsiI TTA'TAA
Cuts at: 608 608
Size: 4867
PstI C_TGCA'G
Cuts at: 1912 2597 1912
Size: 685 4182
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4867
PvuII CAG'CTG
Cuts at: 2290 2650 2290
Size: 360 4507
RsrII CG'GwC_CG
Cuts at: 3060 3060
Size: 4867
SacI G_AGCT'C
Cuts at: 1879 1879
Size: 4867
SalI G'TCGA_C
Cuts at: 1902 1902
Size: 4867
SapI GCTCTTCn'nnn_
Cuts at: 2888 3098 2888
Size: 210 4657
SbfI CC_TGCA'GG
Cuts at: 1912 1912
Size: 4867
ScaI AGT'ACT
Cuts at: 4384 4384
Size: 4867
SexAI A'CCwGG_T
Cuts at: 335 335
Size: 4867
SfcI C'TryA_G
Cuts at: 33 1908 2593 3588 3779 33
Size: 1875 685 995 191 1121
Fragments arranged by size:
1875 1121 995 685 191
SfoI GGC'GCC
Cuts at: 2297 2545 2297
Size: 248 4619
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4867
SmaI CCC'GGG
Cuts at: 1887 1887
Size: 4867
SmlI C'TyrA_G
Cuts at: 157 2020 2935 3429 3691 157
Size: 1863 915 494 262 1333
Fragments arranged by size:
1863 1333 915 494 262
SnaBI TAC'GTA
Cuts at: 1857 1857
Size: 4867
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4867
SphI G_CATG'C
Cuts at: 771 1918 2418 771
Size: 1147 500 3220
Fragments arranged by size:
3220 1147 500
SspI AAT'ATT
Cuts at: 650 650
Size: 4867
StyI C'CwwG_G
Cuts at: 125 1999 2353 125
Size: 1874 354 2639
Fragments arranged by size:
2639 1874 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 337 2448 2581 3040 3251 337
Size: 2111 133 459 211 1953
Fragments arranged by size:
2111 1953 459 211 133
TatI w'GTAC_w
Cuts at: 1120 4382 1120
Size: 3262 1605
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4384 4416 4384
Size: 32 4835
Tth111I GACn'n_nGTC
Cuts at: 373 2662 373
Size: 2289 2578
XbaI T'CTAG_A
Cuts at: 1896 1896
Size: 4867
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2360 2360
Size: 4867
XmaI C'CCGG_G
Cuts at: 1885 1885
Size: 4867
XmnI GAAnn'nnTTC
Cuts at: 733 901 733
Size: 168 4699
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI StuI SwaI XhoI
ZraI