(Circular) (Six-base) MAPSORT of: pF1KSDA1969.seq Check: 841 from: 1 to: 4867 pF1KSDA1969 4867 bp With 182 enzymes: SgfI * June 20, 2008 10:36 .. AccI GT'mk_AC Cuts at: 1903 1903 Size: 4867 Acc65I G'GTAC_C Cuts at: 1881 1881 Size: 4867 AclI AA'CG_TT Cuts at: 4707 4707 Size: 4867 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2690 3122 3871 4139 2690 Size: 432 749 268 3418 Fragments arranged by size: 3418 749 432 268 AflIII A'CryG_T Cuts at: 2217 3323 4300 2217 Size: 1106 977 2784 Fragments arranged by size: 2784 1106 977 AgeI A'CCGG_T Cuts at: 1019 1439 3255 1019 Size: 420 1816 2631 Fragments arranged by size: 2631 1816 420 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 189 189 Size: 4867 AlwNI CAG_nnn'CTG Cuts at: 2170 3739 2170 Size: 1569 3298 ApaLI G'TGCA_C Cuts at: 592 3637 592 Size: 3045 1822 ApoI r'AATT_y Cuts at: 253 297 546 685 797 813 881 1301 1553 Size: 44 249 139 112 16 68 420 252 Cuts at: 1553 1721 1869 4220 4476 253 Size: 168 148 2351 256 644 Fragments arranged by size: 2351 644 420 256 252 249 168 148 139 112 68 44 16 AseI AT'TA_AT Cuts at: 20 20 Size: 4867 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4867 AvaI C'yCGr_G Cuts at: 1885 1885 Size: 4867 BamHI G'GATC_C Cuts at: 1890 1890 Size: 4867 BanI G'GyrC_C Cuts at: 1881 2295 2543 2578 1881 Size: 414 248 35 4170 Fragments arranged by size: 4170 414 248 35 BanII G_rGCy'C Cuts at: 404 1879 2909 404 Size: 1475 1030 2362 Fragments arranged by size: 2362 1475 1030 BbeI G_GCGC'C Cuts at: 2299 2547 2299 Size: 248 4619 BbsI GAAGACnn'nnnn_ Cuts at: 333 1100 333 Size: 767 4100 BciVI GTATCCnnnnn_n' Cuts at: 161 2763 3526 4867 161 Size: 2602 763 1341 161 Fragments arranged by size: 2602 1341 763 161 BclI T'GATC_A Cuts at: 2385 2385 Size: 4867 BglII A'GATC_T Cuts at: 2380 4419 2380 Size: 2039 2828 BlpI GC'TnA_GC Cuts at: 290 1977 290 Size: 1687 3180 Bme1580I G_kGCm'C Cuts at: 596 2490 2583 3641 596 Size: 1894 93 1058 1822 Fragments arranged by size: 1894 1822 1058 93 BmrI ACTGGGnnnn_n' Cuts at: 367 983 2077 2158 2493 4639 367 Size: 616 1094 81 335 2146 595 Fragments arranged by size: 2146 1094 616 595 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1838 3232 1838 Size: 1394 3473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 283 406 911 1079 1163 1247 1331 1415 1499 Size: 123 505 168 84 84 84 84 84 Cuts at: 1499 1583 1833 283 Size: 84 250 3317 Fragments arranged by size: 3317 505 250 168 123 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 142 2041 2920 3414 3712 142 Size: 1899 879 494 298 1297 Fragments arranged by size: 1899 1297 879 494 298 BsaI GGTCTCn'nnnn_ Cuts at: 4571 4571 Size: 4867 BsaAI yAC'GTr Cuts at: 1857 2218 1857 Size: 361 4506 BsaBI GATnn'nnATC Cuts at: 6 2379 4418 6 Size: 2373 2039 455 Fragments arranged by size: 2373 2039 455 BsaHI Gr'CG_yC Cuts at: 2296 2544 3248 4744 2296 Size: 248 704 1496 2419 Fragments arranged by size: 2419 1496 704 248 BsaWI w'CCGG_w Cuts at: 1019 1439 2054 2575 3255 3529 3676 4087 1019 Size: 420 615 521 680 274 147 411 1799 Fragments arranged by size: 1799 680 615 521 420 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 280 280 Size: 4867 BseYI C'CCAG_C Cuts at: 441 2290 3048 3627 4123 441 Size: 1849 758 579 496 1185 Fragments arranged by size: 1849 1185 758 579 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1442 1442 Size: 4867 BsiEI CG_ry'CG Cuts at: 104 2407 2453 3663 104 Size: 2303 46 1210 1308 Fragments arranged by size: 2303 1308 1210 46 BsiHKAI G_wGCw'C Cuts at: 596 1879 2657 2847 3641 596 Size: 1283 778 190 794 1822 Fragments arranged by size: 1822 1283 794 778 190 BsmI GAATG_Cn' Cuts at: 280 280 Size: 4867 Bsp1286I G_dGCh'C Cuts at: 404 596 1879 2490 2583 2657 2847 2909 3641 Size: 192 1283 611 93 74 190 62 732 Cuts at: 3641 404 Size: 1630 Fragments arranged by size: 1630 1283 732 611 192 190 93 74 62 BspEI T'CCGG_A Cuts at: 4087 4087 Size: 4867 BspHI T'CATG_A Cuts at: 4043 4043 Size: 4867 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1915 2129 2431 2812 1915 Size: 214 302 381 3970 Fragments arranged by size: 3970 381 302 214 BsrBI CCG'CTC Cuts at: 50 3157 3211 4697 4862 50 Size: 3107 54 1486 165 55 Fragments arranged by size: 3107 1486 165 55 54 BsrDI GCAATG_nn' Cuts at: 2777 2777 Size: 4867 BsrFI r'CCGG_y Cuts at: 1019 1439 2863 3044 3255 1019 Size: 420 1424 181 211 2631 Fragments arranged by size: 2631 1424 420 211 181 BssSI C'ACGA_G Cuts at: 3136 3496 3136 Size: 360 4507 BstAPI GCAn_nnn'nTGC Cuts at: 2242 2242 Size: 4867 BstBI TT'CG_AA Cuts at: 110 110 Size: 4867 BstYI r'GATC_y Cuts at: 1 1890 2357 2380 2715 2961 3975 4061 4073 Size: 1889 467 23 335 246 1014 86 12 Cuts at: 4073 4419 1 Size: 346 449 Fragments arranged by size: 1889 1014 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 125 125 Size: 4867 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2815 2996 3159 2815 Size: 181 163 4523 Fragments arranged by size: 4523 181 163 BtsI GCAGTG_nn' Cuts at: 2108 2108 Size: 4867 ClaI AT'CG_AT Cuts at: 1188 1188 Size: 4867 DraI TTT'AAA Cuts at: 692 849 1864 692 Size: 157 1015 3695 Fragments arranged by size: 3695 1015 157 DrdI GACnn_nn'nnGTC Cuts at: 2571 3431 2571 Size: 860 4007 EaeI y'GGCC_r Cuts at: 245 475 2450 2624 3015 3042 4389 245 Size: 230 1975 174 391 27 1347 723 Fragments arranged by size: 1975 1347 723 391 230 174 27 EagI C'GGCC_G Cuts at: 2450 2450 Size: 4867 EarI CTCTTCn'nnn_ Cuts at: 409 2888 3098 409 Size: 2479 210 2178 Fragments arranged by size: 2479 2178 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3073 3385 3531 4677 3073 Size: 312 146 1146 3263 Fragments arranged by size: 3263 1146 312 146 EcoICRI GAG'CTC Cuts at: 1877 1877 Size: 4867 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 283 406 911 1079 1163 1247 1331 1415 1499 Size: 123 505 168 84 84 84 84 84 Cuts at: 1499 1583 1833 2690 3122 3871 4139 283 Size: 84 250 857 432 749 268 1011 Fragments arranged by size: 1011 857 749 505 432 268 250 168 123 84 84 84 84 84 84 EcoO109I rG'GnC_Cy Cuts at: 2004 2004 Size: 4867 EcoRI G'AATT_C Cuts at: 253 797 813 881 1301 1553 1869 253 Size: 544 16 68 420 252 316 3251 Fragments arranged by size: 3251 544 420 316 252 68 16 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2884 2884 Size: 4867 FspI TGC'GCA Cuts at: 2646 2646 Size: 4867 HaeII r_GCGC'y Cuts at: 2299 2547 3571 4550 2299 Size: 248 1024 979 2616 Fragments arranged by size: 2616 1024 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2707 2737 2739 2769 2707 Size: 30 2 30 4805 Fragments arranged by size: 4805 30 30 2 HincII GTy'rAC Cuts at: 787 1904 787 Size: 1117 3750 HindIII A'AGCT_T Cuts at: 842 926 1094 1178 1430 1514 1598 842 Size: 84 168 84 252 84 84 4111 Fragments arranged by size: 4111 252 168 84 84 84 84 KasI G'GCGC_C Cuts at: 2295 2543 2295 Size: 248 4619 KpnI G_GTAC'C Cuts at: 1885 1885 Size: 4867 MluI A'CGCG_T Cuts at: 4300 4300 Size: 4867 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3538 3722 3538 Size: 184 4683 MscI TGG'CCA Cuts at: 247 477 2626 247 Size: 230 2149 2488 Fragments arranged by size: 2488 2149 230 MslI CAynn'nnrTG Cuts at: 990 2854 2981 990 Size: 1864 127 2876 Fragments arranged by size: 2876 1864 127 MspA1I CmG'CkG Cuts at: 1976 2290 2650 3665 3910 1976 Size: 314 360 1015 245 2933 Fragments arranged by size: 2933 1015 360 314 245 NaeI GCC'GGC Cuts at: 3046 3046 Size: 4867 NarI GG'CG_CC Cuts at: 2296 2544 2296 Size: 248 4619 NcoI C'CATG_G Cuts at: 125 125 Size: 4867 NgoMIV G'CCGG_C Cuts at: 3044 3044 Size: 4867 NruI TCG'CGA Cuts at: 3228 3228 Size: 4867 NspI r_CATG'y Cuts at: 771 1918 2418 3327 771 Size: 1147 500 909 2311 Fragments arranged by size: 2311 1147 909 500 PasI CC'CwG_GG Cuts at: 178 178 Size: 4867 PciI A'CATG_T Cuts at: 3323 3323 Size: 4867 PmeI GTTT'AAAC Cuts at: 1864 1864 Size: 4867 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4006 4038 4006 Size: 32 4835 PsiI TTA'TAA Cuts at: 608 608 Size: 4867 PstI C_TGCA'G Cuts at: 1912 2597 1912 Size: 685 4182 PvuI CG_AT'CG Cuts at: 104 104 Size: 4867 PvuII CAG'CTG Cuts at: 2290 2650 2290 Size: 360 4507 RsrII CG'GwC_CG Cuts at: 3060 3060 Size: 4867 SacI G_AGCT'C Cuts at: 1879 1879 Size: 4867 SalI G'TCGA_C Cuts at: 1902 1902 Size: 4867 SapI GCTCTTCn'nnn_ Cuts at: 2888 3098 2888 Size: 210 4657 SbfI CC_TGCA'GG Cuts at: 1912 1912 Size: 4867 ScaI AGT'ACT Cuts at: 4384 4384 Size: 4867 SexAI A'CCwGG_T Cuts at: 335 335 Size: 4867 SfcI C'TryA_G Cuts at: 33 1908 2593 3588 3779 33 Size: 1875 685 995 191 1121 Fragments arranged by size: 1875 1121 995 685 191 SfoI GGC'GCC Cuts at: 2297 2545 2297 Size: 248 4619 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4867 SmaI CCC'GGG Cuts at: 1887 1887 Size: 4867 SmlI C'TyrA_G Cuts at: 157 2020 2935 3429 3691 157 Size: 1863 915 494 262 1333 Fragments arranged by size: 1863 1333 915 494 262 SnaBI TAC'GTA Cuts at: 1857 1857 Size: 4867 SpeI A'CTAG_T Cuts at: 67 67 Size: 4867 SphI G_CATG'C Cuts at: 771 1918 2418 771 Size: 1147 500 3220 Fragments arranged by size: 3220 1147 500 SspI AAT'ATT Cuts at: 650 650 Size: 4867 StyI C'CwwG_G Cuts at: 125 1999 2353 125 Size: 1874 354 2639 Fragments arranged by size: 2639 1874 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 337 2448 2581 3040 3251 337 Size: 2111 133 459 211 1953 Fragments arranged by size: 2111 1953 459 211 133 TatI w'GTAC_w Cuts at: 1120 4382 1120 Size: 3262 1605 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4384 4416 4384 Size: 32 4835 Tth111I GACn'n_nGTC Cuts at: 373 2662 373 Size: 2289 2578 XbaI T'CTAG_A Cuts at: 1896 1896 Size: 4867 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2360 2360 Size: 4867 XmaI C'CCGG_G Cuts at: 1885 1885 Size: 4867 XmnI GAAnn'nnTTC Cuts at: 733 901 733 Size: 168 4699 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NsiI PacI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SrfI StuI SwaI XhoI ZraI