Restriction map for
pF1KSDA1971
(Circular) (Six-base) MAPSORT of: pF1KSDA1971.seq Check: 7157 from: 1 to: 5566
pF1KSDA1971 5566 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2211 2211
Size: 5566
AccI GT'mk_AC
Cuts at: 2602 2602
Size: 5566
Acc65I G'GTAC_C
Cuts at: 2580 2580
Size: 5566
AclI AA'CG_TT
Cuts at: 782 5406 782
Size: 4624 942
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1010 1126 1646 2404 3389 3821 4570 4838
Size: 21 116 520 758 985 432 749 268
Cuts at: 4838 989
Size: 1717
Fragments arranged by size:
1717 985 758 749 520 432 268 116
21
AfeI AGC'GCT
Cuts at: 433 433
Size: 5566
AflII C'TTAA_G
Cuts at: 2274 2274
Size: 5566
AflIII A'CryG_T
Cuts at: 2916 4022 4999 2916
Size: 1106 977 3483
Fragments arranged by size:
3483 1106 977
AgeI A'CCGG_T
Cuts at: 3954 3954
Size: 5566
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 229
Size: 32 5534
AlwNI CAG_nnn'CTG
Cuts at: 558 1527 1550 1810 2869 4438 558
Size: 969 23 260 1059 1569 1686
Fragments arranged by size:
1686 1569 1059 969 260 23
ApaI G_GGCC'C
Cuts at: 303 314 303
Size: 11 5555
ApaLI G'TGCA_C
Cuts at: 4336 4336
Size: 5566
ApoI r'AATT_y
Cuts at: 1217 1298 1324 1488 2568 4919 5175 1217
Size: 81 26 164 1080 2351 256 1608
Fragments arranged by size:
2351 1608 1080 256 164 81 26
AseI AT'TA_AT
Cuts at: 20 775 20
Size: 755 4811
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5566
AvaI C'yCGr_G
Cuts at: 193 313 319 496 1157 2584 193
Size: 120 6 177 661 1427 3175
Fragments arranged by size:
3175 1427 661 177 120 6
AvrII C'CTAG_G
Cuts at: 930 930
Size: 5566
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 252 285 1632 1665 252
Size: 33 1347 33 4153
Fragments arranged by size:
4153 1347 33 33
BamHI G'GATC_C
Cuts at: 2589 2589
Size: 5566
BanI G'GyrC_C
Cuts at: 162 200 610 2580 2994 3242 3277 162
Size: 38 410 1970 414 248 35 2451
Fragments arranged by size:
2451 1970 414 410 248 38 35
BanII G_rGCy'C
Cuts at: 194 303 314 320 326 386 497 2160 2292
Size: 109 11 6 6 60 111 1663 132
Cuts at: 2292 2578 3608 194
Size: 286 1030 2152
Fragments arranged by size:
2152 1663 1030 286 132 111 109 60
11 6 6
BbeI G_GCGC'C
Cuts at: 614 2998 3246 614
Size: 2384 248 2934
Fragments arranged by size:
2934 2384 248
BbvCI CC'TCA_GC
Cuts at: 641 641
Size: 5566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2185 2219 2185
Size: 34 5532
BciVI GTATCCnnnnn_n'
Cuts at: 3462 4225 5566 3462
Size: 763 1341 3462
Fragments arranged by size:
3462 1341 763
BclI T'GATC_A
Cuts at: 2001 3084 2001
Size: 1083 4483
BglI GCCn_nnn'nGGC
Cuts at: 155 198 155
Size: 43 5523
BglII A'GATC_T
Cuts at: 1236 3079 5118 1236
Size: 1843 2039 1684
Fragments arranged by size:
2039 1843 1684
BlpI GC'TnA_GC
Cuts at: 2207 2676 2207
Size: 469 5097
Bme1580I G_kGCm'C
Cuts at: 303 314 3189 3282 4340 303
Size: 11 2875 93 1058 1529
Fragments arranged by size:
2875 1529 1058 93 11
BmgBI CAC'GTC
Cuts at: 465 2182 465
Size: 1717 3849
BmrI ACTGGGnnnn_n'
Cuts at: 2776 2857 3192 5338 2776
Size: 81 335 2146 3004
Fragments arranged by size:
3004 2146 335 81
BmtI G_CTAG'C
Cuts at: 1156 1156
Size: 5566
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1585 2311 3931 1585
Size: 726 1620 3220
Fragments arranged by size:
3220 1620 726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 448 976 1549 2144 2464 172
Size: 276 528 573 595 320 3274
Fragments arranged by size:
3274 595 573 528 320 276
Bpu10I CC'TnA_GC
Cuts at: 641 641
Size: 5566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2476 2740 3619 4113 4411 2108
Size: 368 264 879 494 298 3263
Fragments arranged by size:
3263 879 494 368 298 264
BsaI GGTCTCn'nnnn_
Cuts at: 5270 5270
Size: 5566
BsaAI yAC'GTr
Cuts at: 2218 2556 2917 2218
Size: 338 361 4867
Fragments arranged by size:
4867 361 338
BsaBI GATnn'nnATC
Cuts at: 6 1752 3078 5117 6
Size: 1746 1326 2039 455
Fragments arranged by size:
2039 1746 1326 455
BsaHI Gr'CG_yC
Cuts at: 611 2995 3243 3947 5443 611
Size: 2384 248 704 1496 734
Fragments arranged by size:
2384 1496 734 704 248
BsaWI w'CCGG_w
Cuts at: 1571 2297 2753 3274 3954 4228 4375 4786 1571
Size: 726 456 521 680 274 147 411 2351
Fragments arranged by size:
2351 726 680 521 456 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 229 259 229
Size: 30 5536
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1708 2039 2042 2045 2087 2282 2475 2527 1708
Size: 331 3 3 42 195 193 52 4747
Fragments arranged by size:
4747 331 195 193 52 42 3 3
BseYI C'CCAG_C
Cuts at: 158 356 1841 2416 2989 3747 4326 4822 158
Size: 198 1485 575 573 758 579 496 902
Fragments arranged by size:
1485 902 758 579 575 573 496 198
BsiEI CG_ry'CG
Cuts at: 104 267 377 596 656 3106 3152 4362 104
Size: 163 110 219 60 2450 46 1210 1308
Fragments arranged by size:
2450 1308 1210 219 163 110 60 46
BsiHKAI G_wGCw'C
Cuts at: 2109 2292 2351 2578 3356 3546 4340 2109
Size: 183 59 227 778 190 794 3335
Fragments arranged by size:
3335 794 778 227 190 183 59
BsmI GAATG_Cn'
Cuts at: 1067 1067
Size: 5566
BsmBI CGTCTCn'nnnn_
Cuts at: 348 1008 348
Size: 660 4906
Bsp1286I G_dGCh'C
Cuts at: 194 303 314 320 326 386 497 2109 2160
Size: 109 11 6 6 60 111 1612 51
Cuts at: 2160 2292 2351 2578 3189 3282 3356 3546 3608
Size: 132 59 227 611 93 74 190 62
Cuts at: 3608 4340 194
Size: 732 1420
Fragments arranged by size:
1612 1420 732 611 227 190 132 111
109 93 74 62 60 59 51 11
6 6
BspEI T'CCGG_A
Cuts at: 1571 2297 4786 1571
Size: 726 2489 2351
Fragments arranged by size:
2489 2351 726
BspHI T'CATG_A
Cuts at: 1175 4742 1175
Size: 3567 1999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1832 2211 2614 2828 3130 3511 1832
Size: 379 403 214 302 381 3887
Fragments arranged by size:
3887 403 381 379 302 214
BsrBI CCG'CTC
Cuts at: 50 687 3856 3910 5396 5561 50
Size: 637 3169 54 1486 165 55
Fragments arranged by size:
3169 1486 637 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3476 3476
Size: 5566
BsrFI r'CCGG_y
Cuts at: 165 203 361 398 2313 3562 3743 3954 165
Size: 38 158 37 1915 1249 181 211 1777
Fragments arranged by size:
1915 1777 1249 211 181 158 38 37
BssHII G'CGCG_C
Cuts at: 392 525 696 698 392
Size: 133 171 2 5260
Fragments arranged by size:
5260 171 133 2
BssSI C'ACGA_G
Cuts at: 3835 4195 3835
Size: 360 5206
BstAPI GCAn_nnn'nTGC
Cuts at: 207 2941 207
Size: 2734 2832
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5566
BstEII G'GTnAC_C
Cuts at: 819 819
Size: 5566
BstXI CCAn_nnnn'nTGG
Cuts at: 765 2548 765
Size: 1783 3783
BstYI r'GATC_y
Cuts at: 1 1236 1547 2248 2589 3056 3079 3414 3660
Size: 1235 311 701 341 467 23 335 246
Cuts at: 3660 4674 4760 4772 5118 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1235 1014 701 467 449 346 341 335
311 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 332 332
Size: 5566
BtgI C'CryG_G
Cuts at: 125 377 387 442 764 824 125
Size: 252 10 55 322 60 4867
Fragments arranged by size:
4867 322 252 60 55 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3514 3695 3858 3514
Size: 181 163 5222
Fragments arranged by size:
5222 181 163
BtsI GCAGTG_nn'
Cuts at: 1522 2035 2807 1522
Size: 513 772 4281
Fragments arranged by size:
4281 772 513
DraI TTT'AAA
Cuts at: 1199 1761 2563 1199
Size: 562 802 4202
Fragments arranged by size:
4202 802 562
DraIII CAC_nnn'GTG
Cuts at: 1872 1872
Size: 5566
DrdI GACnn_nn'nnGTC
Cuts at: 3270 4130 3270
Size: 860 4706
EaeI y'GGCC_r
Cuts at: 374 418 517 593 653 2538 3149 3323 3714
Size: 44 99 76 60 1885 611 174 391
Cuts at: 3714 3741 5088 374
Size: 27 1347 852
Fragments arranged by size:
1885 1347 852 611 391 174 99 76
60 44 27
EagI C'GGCC_G
Cuts at: 374 593 653 3149 374
Size: 219 60 2496 2791
Fragments arranged by size:
2791 2496 219 60
EarI CTCTTCn'nnn_
Cuts at: 187 637 1084 1685 3587 3797 187
Size: 450 447 601 1902 210 1956
Fragments arranged by size:
1956 1902 601 450 447 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 730 3772 4084 4230 5376 730
Size: 3042 312 146 1146 920
Fragments arranged by size:
3042 1146 920 312 146
EcoICRI GAG'CTC
Cuts at: 2290 2576 2290
Size: 286 5280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 448 976 989 1010 1126 1549 1646 2144
Size: 276 528 13 21 116 423 97 498
Cuts at: 2144 2404 2464 3389 3821 4570 4838 172
Size: 260 60 925 432 749 268 900
Fragments arranged by size:
925 900 749 528 498 432 423 276
268 260 116 97 60 21 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2274 2274
Size: 5566
EcoO109I rG'GnC_Cy
Cuts at: 177 299 310 479 532 934 2532 2703 177
Size: 122 11 169 53 402 1598 171 3040
Fragments arranged by size:
3040 1598 402 171 169 122 53 11
EcoRI G'AATT_C
Cuts at: 2568 2568
Size: 5566
EcoRV GAT'ATC
Cuts at: 1017 1017
Size: 5566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3583 3583
Size: 5566
FseI GG_CCGG'CC
Cuts at: 365 365
Size: 5566
FspI TGC'GCA
Cuts at: 3345 3345
Size: 5566
HaeII r_GCGC'y
Cuts at: 415 435 511 550 614 2998 3246 4270 5249
Size: 20 76 39 64 2384 248 1024 979
Cuts at: 5249 415
Size: 732
Fragments arranged by size:
2384 1024 979 732 248 76 64 39
20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1083 1115 1221 1253 2017 2049 3406 3436 3438
Size: 32 106 32 764 32 1357 30 2
Cuts at: 3438 3468 1083
Size: 30 3181
Fragments arranged by size:
3181 1357 764 106 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2603 2603
Size: 5566
HindIII A'AGCT_T
Cuts at: 895 895
Size: 5566
KasI G'GCGC_C
Cuts at: 610 2994 3242 610
Size: 2384 248 2934
Fragments arranged by size:
2934 2384 248
KpnI G_GTAC'C
Cuts at: 2584 2584
Size: 5566
MluI A'CGCG_T
Cuts at: 4999 4999
Size: 5566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1112 1349 1767 4237 4421 1112
Size: 237 418 2470 184 2257
Fragments arranged by size:
2470 2257 418 237 184
MscI TGG'CCA
Cuts at: 2540 3325 2540
Size: 785 4781
MslI CAynn'nnrTG
Cuts at: 3553 3680 3553
Size: 127 5439
MspA1I CmG'CkG
Cuts at: 282 356 379 389 444 570 1527 2675 2989
Size: 74 23 10 55 126 957 1148 314
Cuts at: 2989 3349 4364 4609 282
Size: 360 1015 245 1239
Fragments arranged by size:
1239 1148 1015 957 360 314 245 126
74 55 23 10
NaeI GCC'GGC
Cuts at: 363 2315 3745 363
Size: 1952 1430 2184
Fragments arranged by size:
2184 1952 1430
NarI GG'CG_CC
Cuts at: 611 2995 3243 611
Size: 2384 248 2934
Fragments arranged by size:
2934 2384 248
NcoI C'CATG_G
Cuts at: 125 764 125
Size: 639 4927
NgoMIV G'CCGG_C
Cuts at: 361 2313 3743 361
Size: 1952 1430 2184
Fragments arranged by size:
2184 1952 1430
NheI G'CTAG_C
Cuts at: 1152 1152
Size: 5566
NruI TCG'CGA
Cuts at: 3927 3927
Size: 5566
NspI r_CATG'y
Cuts at: 2617 3117 4026 2617
Size: 500 909 4157
Fragments arranged by size:
4157 909 500
PciI A'CATG_T
Cuts at: 4022 4022
Size: 5566
PflMI CCAn_nnn'nTGG
Cuts at: 816 1527 816
Size: 711 4855
PfoI T'CCnGG_A
Cuts at: 170 680 2244 170
Size: 510 1564 3492
Fragments arranged by size:
3492 1564 510
PmeI GTTT'AAAC
Cuts at: 2563 2563
Size: 5566
PmlI CAC'GTG
Cuts at: 2218 2218
Size: 5566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 229 261 4705 4737 229
Size: 32 4444 32 1058
Fragments arranged by size:
4444 1058 32 32
PpuMI rG'GwC_Cy
Cuts at: 532 934 2532 532
Size: 402 1598 3566
Fragments arranged by size:
3566 1598 402
PshAI GACnn'nnGTC
Cuts at: 932 932
Size: 5566
PsiI TTA'TAA
Cuts at: 1390 1390
Size: 5566
PspOMI G'GGCC_C
Cuts at: 299 310 299
Size: 11 5555
PstI C_TGCA'G
Cuts at: 554 1478 2611 3296 554
Size: 924 1133 685 2824
Fragments arranged by size:
2824 1133 924 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5566
PvuII CAG'CTG
Cuts at: 356 570 1527 2989 3349 356
Size: 214 957 1462 360 2573
Fragments arranged by size:
2573 1462 957 360 214
RsrII CG'GwC_CG
Cuts at: 168 3759 168
Size: 3591 1975
SacI G_AGCT'C
Cuts at: 2292 2578 2292
Size: 286 5280
SacII CC_GC'GG
Cuts at: 380 390 445 380
Size: 10 55 5501
Fragments arranged by size:
5501 55 10
SalI G'TCGA_C
Cuts at: 2601 2601
Size: 5566
SanDI GG'GwC_CC
Cuts at: 532 532
Size: 5566
SapI GCTCTTCn'nnn_
Cuts at: 3587 3797 3587
Size: 210 5356
SbfI CC_TGCA'GG
Cuts at: 2611 2611
Size: 5566
ScaI AGT'ACT
Cuts at: 847 5083 847
Size: 4236 1330
SfcI C'TryA_G
Cuts at: 33 550 1474 2607 3292 4287 4478 33
Size: 517 924 1133 685 995 191 1121
Fragments arranged by size:
1133 1121 995 924 685 517 191
SfoI GGC'GCC
Cuts at: 612 2996 3244 612
Size: 2384 248 2934
Fragments arranged by size:
2934 2384 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5566
SmaI CCC'GGG
Cuts at: 2586 2586
Size: 5566
SmlI C'TyrA_G
Cuts at: 1157 2123 2274 2455 2719 3634 4128 4390 1157
Size: 966 151 181 264 915 494 262 2333
Fragments arranged by size:
2333 966 915 494 264 262 181 151
SnaBI TAC'GTA
Cuts at: 2556 2556
Size: 5566
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5566
SphI G_CATG'C
Cuts at: 2617 3117 2617
Size: 500 5066
StyI C'CwwG_G
Cuts at: 125 764 930 2225 2698 3052 125
Size: 639 166 1295 473 354 2639
Fragments arranged by size:
2639 1295 639 473 354 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 150 3147 3280 3739 3950 150
Size: 2997 133 459 211 1766
Fragments arranged by size:
2997 1766 459 211 133
TatI w'GTAC_w
Cuts at: 845 5081 845
Size: 4236 1330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1107 1139 5083 5115 1107
Size: 32 3944 32 1558
Fragments arranged by size:
3944 1558 32 32
Tth111I GACn'n_nGTC
Cuts at: 3361 3361
Size: 5566
XbaI T'CTAG_A
Cuts at: 2595 2595
Size: 5566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3059 3059
Size: 5566
XhoI C'TCGA_G
Cuts at: 1157 1157
Size: 5566
XmaI C'CCGG_G
Cuts at: 2584 2584
Size: 5566
XmnI GAAnn'nnTTC
Cuts at: 1139 1238 1139
Size: 99 5467
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AlfI AscI BbsI BfrBI BsgI
BsiWI BsrGI BstZ17I ClaI CspCI FspAI HpaI MfeI
NdeI NotI NsiI PacI PasI PspXI PsrI SexAI
SfiI SgrAI SrfI SspI StuI SwaI ZraI