Restriction map for
pF1KSDA1972
(Circular) (Six-base) MAPSORT of: pF1KSDA1972.seq Check: 4248 from: 1 to: 4865
pF1KSDA1972 4865 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1041 1903 1041
Size: 862 4003
Acc65I G'GTAC_C
Cuts at: 1881 1881
Size: 4865
AclI AA'CG_TT
Cuts at: 4705 4705
Size: 4865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1138 1652 2690 3122 3869 4137 847
Size: 291 514 1038 432 747 268 1575
Fragments arranged by size:
1575 1038 747 514 432 291 268
AflIII A'CryG_T
Cuts at: 2217 3321 4298 2217
Size: 1104 977 2784
Fragments arranged by size:
2784 1104 977
AgeI A'CCGG_T
Cuts at: 3253 3253
Size: 4865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1483 1483
Size: 4865
AlwNI CAG_nnn'CTG
Cuts at: 180 772 825 2170 3737 180
Size: 592 53 1345 1567 1308
Fragments arranged by size:
1567 1345 1308 592 53
ApaLI G'TGCA_C
Cuts at: 3635 3635
Size: 4865
ApoI r'AATT_y
Cuts at: 665 1273 1594 1869 4218 4474 665
Size: 608 321 275 2349 256 1056
Fragments arranged by size:
2349 1056 608 321 275 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4865
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4865
AvaI C'yCGr_G
Cuts at: 1262 1885 1262
Size: 623 4242
AvrII C'CTAG_G
Cuts at: 216 741 216
Size: 525 4340
BamHI G'GATC_C
Cuts at: 1890 1890
Size: 4865
BanI G'GyrC_C
Cuts at: 229 1881 2295 2543 2578 229
Size: 1652 414 248 35 2516
Fragments arranged by size:
2516 1652 414 248 35
BanII G_rGCy'C
Cuts at: 1879 2909 1879
Size: 1030 3835
BbeI G_GCGC'C
Cuts at: 2299 2547 2299
Size: 248 4617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 304 338 1682 1716 304
Size: 34 1344 34 3453
Fragments arranged by size:
3453 1344 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1699 2763 3524 4865 1699
Size: 1064 761 1341 1699
Fragments arranged by size:
1699 1341 1064 761
BclI T'GATC_A
Cuts at: 477 2385 477
Size: 1908 2957
BglII A'GATC_T
Cuts at: 1121 2380 4417 1121
Size: 1259 2037 1569
Fragments arranged by size:
2037 1569 1259
BlpI GC'TnA_GC
Cuts at: 1977 1977
Size: 4865
Bme1580I G_kGCm'C
Cuts at: 1005 2490 2583 3639 1005
Size: 1485 93 1056 2231
Fragments arranged by size:
2231 1485 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 598 999 2077 2158 2493 4637 598
Size: 401 1078 81 335 2144 826
Fragments arranged by size:
2144 1078 826 401 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3232 3232
Size: 4865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 634 1810 245
Size: 389 1176 3300
Fragments arranged by size:
3300 1176 389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 1089 2041 2920 3412 3710 687
Size: 402 952 879 492 298 1842
Fragments arranged by size:
1842 952 879 492 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 1786 4569 1786
Size: 2783 2082
BsaAI yAC'GTr
Cuts at: 1857 2218 1857
Size: 361 4504
BsaBI GATnn'nnATC
Cuts at: 6 476 2379 4416 6
Size: 470 1903 2037 455
Fragments arranged by size:
2037 1903 470 455
BsaHI Gr'CG_yC
Cuts at: 1238 1662 2296 2544 3246 4742 1238
Size: 424 634 248 702 1496 1361
Fragments arranged by size:
1496 1361 702 634 424 248
BsaWI w'CCGG_w
Cuts at: 2054 2575 3253 3527 3674 4085 2054
Size: 521 678 274 147 411 2834
Fragments arranged by size:
2834 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1391 1421 1391
Size: 30 4835
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1149 1149
Size: 4865
BseYI C'CCAG_C
Cuts at: 1253 2290 3048 3625 4121 1253
Size: 1037 758 577 496 1997
Fragments arranged by size:
1997 1037 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 344
Size: 4865
BsiEI CG_ry'CG
Cuts at: 104 2407 2453 3661 104
Size: 2303 46 1208 1308
Fragments arranged by size:
2303 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 203 1879 2657 2847 3639 203
Size: 1676 778 190 792 1429
Fragments arranged by size:
1676 1429 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 203 1005 1879 2490 2583 2657 2847 2909 3639
Size: 802 874 611 93 74 190 62 730
Cuts at: 3639 203
Size: 1429
Fragments arranged by size:
1429 874 802 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4085 4085
Size: 4865
BspHI T'CATG_A
Cuts at: 396 1284 4041 396
Size: 888 2757 1220
Fragments arranged by size:
2757 1220 888
BspMI ACCTGCnnnn'nnnn_
Cuts at: 757 1804 1915 2129 2431 2812 757
Size: 1047 111 214 302 381 2810
Fragments arranged by size:
2810 1047 381 302 214 111
BsrBI CCG'CTC
Cuts at: 50 3157 3211 4695 4860 50
Size: 3107 54 1484 165 55
Fragments arranged by size:
3107 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 628 1104 2777 628
Size: 476 1673 2716
Fragments arranged by size:
2716 1673 476
BsrFI r'CCGG_y
Cuts at: 935 1337 2863 3044 3253 935
Size: 402 1526 181 209 2547
Fragments arranged by size:
2547 1526 402 209 181
BssSI C'ACGA_G
Cuts at: 3136 3494 3136
Size: 358 4507
BstAPI GCAn_nnn'nTGC
Cuts at: 772 2242 772
Size: 1470 3395
BstBI TT'CG_AA
Cuts at: 110 3226 110
Size: 3116 1749
BstYI r'GATC_y
Cuts at: 1 582 1121 1890 2357 2380 2715 2961 3973
Size: 581 539 769 467 23 335 246 1012
Cuts at: 3973 4059 4071 4417 1
Size: 86 12 346 449
Fragments arranged by size:
1012 769 581 539 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 242 355 242
Size: 113 4752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2815 2996 3159 2815
Size: 181 163 4521
Fragments arranged by size:
4521 181 163
BtsI GCAGTG_nn'
Cuts at: 332 2108 332
Size: 1776 3089
DraI TTT'AAA
Cuts at: 677 1031 1454 1864 677
Size: 354 423 410 3678
Fragments arranged by size:
3678 423 410 354
DrdI GACnn_nn'nnGTC
Cuts at: 940 2571 3429 940
Size: 1631 858 2376
Fragments arranged by size:
2376 1631 858
EaeI y'GGCC_r
Cuts at: 171 370 752 2450 2624 3015 3042 4387 171
Size: 199 382 1698 174 391 27 1345 649
Fragments arranged by size:
1698 1345 649 391 382 199 174 27
EagI C'GGCC_G
Cuts at: 2450 2450
Size: 4865
EarI CTCTTCn'nnn_
Cuts at: 191 2888 3098 191
Size: 2697 210 1958
Fragments arranged by size:
2697 1958 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3073 3383 3529 4675 3073
Size: 310 146 1146 3263
Fragments arranged by size:
3263 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1877 1877
Size: 4865
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 634 847 1138 1652 1810 2690 3122 3869
Size: 389 213 291 514 158 880 432 747
Cuts at: 3869 4137 245
Size: 268 973
Fragments arranged by size:
973 880 747 514 432 389 291 268
213 158
EcoNI CCTnn'n_nnAGG
Cuts at: 172 172
Size: 4865
EcoO109I rG'GnC_Cy
Cuts at: 360 391 769 2004 360
Size: 31 378 1235 3221
Fragments arranged by size:
3221 1235 378 31
EcoRI G'AATT_C
Cuts at: 1869 1869
Size: 4865
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1503 2884 1503
Size: 1381 3484
FspI TGC'GCA
Cuts at: 2646 2646
Size: 4865
HaeII r_GCGC'y
Cuts at: 2299 2547 3569 4548 2299
Size: 248 1022 979 2616
Fragments arranged by size:
2616 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1113 1144 1145 1176 1300 1332 2707 2737 2739
Size: 31 1 31 124 32 1375 30 2
Cuts at: 2739 2769 1113
Size: 30 3209
Fragments arranged by size:
3209 1375 124 32 31 31 30 30
2 1
HincII GTy'rAC
Cuts at: 188 294 1904 188
Size: 106 1610 3149
Fragments arranged by size:
3149 1610 106
KasI G'GCGC_C
Cuts at: 2295 2543 2295
Size: 248 4617
KpnI G_GTAC'C
Cuts at: 1885 1885
Size: 4865
MfeI C'AATT_G
Cuts at: 1738 1738
Size: 4865
MluI A'CGCG_T
Cuts at: 4298 4298
Size: 4865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 419 521 3536 3720 419
Size: 102 3015 184 1564
Fragments arranged by size:
3015 1564 184 102
MscI TGG'CCA
Cuts at: 173 2626 173
Size: 2453 2412
MslI CAynn'nnrTG
Cuts at: 850 2854 2981 850
Size: 2004 127 2734
Fragments arranged by size:
2734 2004 127
MspA1I CmG'CkG
Cuts at: 598 1047 1344 1467 1630 1788 1976 2290 2650
Size: 449 297 123 163 158 188 314 360
Cuts at: 2650 3663 3908 598
Size: 1013 245 1555
Fragments arranged by size:
1555 1013 449 360 314 297 245 188
163 158 123
NaeI GCC'GGC
Cuts at: 1339 3046 1339
Size: 1707 3158
NarI GG'CG_CC
Cuts at: 2296 2544 2296
Size: 248 4617
NcoI C'CATG_G
Cuts at: 242 242
Size: 4865
NgoMIV G'CCGG_C
Cuts at: 1337 3044 1337
Size: 1707 3158
NspI r_CATG'y
Cuts at: 1918 2418 3325 1918
Size: 500 907 3458
Fragments arranged by size:
3458 907 500
PciI A'CATG_T
Cuts at: 3321 3321
Size: 4865
PmeI GTTT'AAAC
Cuts at: 1864 1864
Size: 4865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 4004
Size: 32 4833
PpuMI rG'GwC_Cy
Cuts at: 360 391 769 360
Size: 31 378 4456
Fragments arranged by size:
4456 378 31
PsiI TTA'TAA
Cuts at: 604 604
Size: 4865
PstI C_TGCA'G
Cuts at: 738 1521 1912 2597 738
Size: 783 391 685 3006
Fragments arranged by size:
3006 783 685 391
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4865
PvuII CAG'CTG
Cuts at: 598 1047 1344 1467 1630 1788 2290 2650 598
Size: 449 297 123 163 158 502 360 2813
Fragments arranged by size:
2813 502 449 360 297 163 158 123
RsrII CG'GwC_CG
Cuts at: 3060 3060
Size: 4865
SacI G_AGCT'C
Cuts at: 1879 1879
Size: 4865
SalI G'TCGA_C
Cuts at: 1902 1902
Size: 4865
SanDI GG'GwC_CC
Cuts at: 360 360
Size: 4865
SapI GCTCTTCn'nnn_
Cuts at: 191 2888 3098 191
Size: 2697 210 1958
Fragments arranged by size:
2697 1958 210
SbfI CC_TGCA'GG
Cuts at: 1912 1912
Size: 4865
ScaI AGT'ACT
Cuts at: 4382 4382
Size: 4865
SfcI C'TryA_G
Cuts at: 33 734 817 1517 1908 2593 3586 3777 33
Size: 701 83 700 391 685 993 191 1121
Fragments arranged by size:
1121 993 701 700 685 391 191 83
SfoI GGC'GCC
Cuts at: 2297 2545 2297
Size: 248 4617
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4865
SmaI CCC'GGG
Cuts at: 1887 1887
Size: 4865
SmlI C'TyrA_G
Cuts at: 702 1068 1262 2020 2935 3427 3689 702
Size: 366 194 758 915 492 262 1878
Fragments arranged by size:
1878 915 758 492 366 262 194
SnaBI TAC'GTA
Cuts at: 1857 1857
Size: 4865
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4865
SphI G_CATG'C
Cuts at: 1918 2418 1918
Size: 500 4365
StyI C'CwwG_G
Cuts at: 168 216 242 376 403 741 1651 1999 2353
Size: 48 26 134 27 338 910 348 354
Cuts at: 2353 168
Size: 2680
Fragments arranged by size:
2680 910 354 348 338 134 48 27
26
SwaI ATTT'AAAT
Cuts at: 677 677
Size: 4865
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 313 1397 2448 2581 3040 3249 313
Size: 1084 1051 133 459 209 1929
Fragments arranged by size:
1929 1084 1051 459 209 133
TatI w'GTAC_w
Cuts at: 4380 4380
Size: 4865
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 213 245 4382 4414 213
Size: 32 4137 32 664
Fragments arranged by size:
4137 664 32 32
Tth111I GACn'n_nGTC
Cuts at: 2662 2662
Size: 4865
XbaI T'CTAG_A
Cuts at: 1825 1896 1825
Size: 71 4794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2360 2360
Size: 4865
XhoI C'TCGA_G
Cuts at: 1262 1262
Size: 4865
XmaI C'CCGG_G
Cuts at: 1885 1885
Size: 4865
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SrfI SspI StuI XmnI
ZraI