Restriction map for
pF1KSDA1993
(Circular) (Six-base) MAPSORT of: pF1KSDA1993.seq Check: 4247 from: 1 to: 4651
pF1KSDA1993 4651 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1133 1133
Size: 4651
AccI GT'mk_AC
Cuts at: 1687 1687
Size: 4651
Acc65I G'GTAC_C
Cuts at: 905 1232 1459 1665 905
Size: 327 227 206 3891
Fragments arranged by size:
3891 327 227 206
AclI AA'CG_TT
Cuts at: 4491 4491
Size: 4651
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 448 581 1523 2474 2906 3655 3923 445
Size: 3 133 942 951 432 749 268 1173
Fragments arranged by size:
1173 951 942 749 432 268 133 3
AflIII A'CryG_T
Cuts at: 2001 3107 4084 2001
Size: 1106 977 2568
Fragments arranged by size:
2568 1106 977
AgeI A'CCGG_T
Cuts at: 3039 3039
Size: 4651
AleI CACnn'nnGTG
Cuts at: 835 835
Size: 4651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 721 721
Size: 4651
AlwNI CAG_nnn'CTG
Cuts at: 1146 1624 1954 3523 1146
Size: 478 330 1569 2274
Fragments arranged by size:
2274 1569 478 330
ApaLI G'TGCA_C
Cuts at: 489 3421 489
Size: 2932 1719
ApoI r'AATT_y
Cuts at: 1610 1653 4004 4260 1610
Size: 43 2351 256 2001
Fragments arranged by size:
2351 2001 256 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 4651
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4651
AvaI C'yCGr_G
Cuts at: 172 571 1186 1205 1528 1669 172
Size: 399 615 19 323 141 3154
Fragments arranged by size:
3154 615 399 323 141 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1004 1037 1369 1402 1004
Size: 33 332 33 4253
Fragments arranged by size:
4253 332 33 33
BamHI G'GATC_C
Cuts at: 1674 1674
Size: 4651
BanI G'GyrC_C
Cuts at: 905 1232 1459 1665 2079 2327 2362 905
Size: 327 227 206 414 248 35 3194
Fragments arranged by size:
3194 414 327 248 227 206 35
BanII G_rGCy'C
Cuts at: 273 958 1159 1663 2693 273
Size: 685 201 504 1030 2231
Fragments arranged by size:
2231 1030 685 504 201
BbeI G_GCGC'C
Cuts at: 2083 2331 2083
Size: 248 4403
BciVI GTATCCnnnnn_n'
Cuts at: 2547 3310 4651 2547
Size: 763 1341 2547
Fragments arranged by size:
2547 1341 763
BclI T'GATC_A
Cuts at: 2169 2169
Size: 4651
BglII A'GATC_T
Cuts at: 623 1430 2164 4203 623
Size: 807 734 2039 1071
Fragments arranged by size:
2039 1071 807 734
BlpI GC'TnA_GC
Cuts at: 1072 1761 1072
Size: 689 3962
Bme1580I G_kGCm'C
Cuts at: 173 493 734 1098 2274 2367 3425 173
Size: 320 241 364 1176 93 1058 1399
Fragments arranged by size:
1399 1176 1058 364 320 241 93
BmrI ACTGGGnnnn_n'
Cuts at: 610 1861 1942 2277 4423 610
Size: 1251 81 335 2146 838
Fragments arranged by size:
2146 1251 838 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 750 3016 750
Size: 2266 2385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1402 958
Size: 444 4207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1825 2704 3198 3496 1825
Size: 879 494 298 2980
Fragments arranged by size:
2980 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 782 797 4355 782
Size: 15 3558 1078
Fragments arranged by size:
3558 1078 15
BsaAI yAC'GTr
Cuts at: 1548 1641 2002 1548
Size: 93 361 4197
Fragments arranged by size:
4197 361 93
BsaBI GATnn'nnATC
Cuts at: 6 2163 4202 6
Size: 2157 2039 455
Fragments arranged by size:
2157 2039 455
BsaHI Gr'CG_yC
Cuts at: 2080 2328 3032 4528 2080
Size: 248 704 1496 2203
Fragments arranged by size:
2203 1496 704 248
BsaWI w'CCGG_w
Cuts at: 677 1838 2359 3039 3313 3460 3871 677
Size: 1161 521 680 274 147 411 1457
Fragments arranged by size:
1457 1161 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 871 901 970 1000 871
Size: 30 69 30 4522
Fragments arranged by size:
4522 69 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 620 739 876 1100 1227 620
Size: 119 137 224 127 4044
Fragments arranged by size:
4044 224 137 127 119
BseYI C'CCAG_C
Cuts at: 694 868 2074 2832 3411 3907 694
Size: 174 1206 758 579 496 1438
Fragments arranged by size:
1438 1206 758 579 496 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1171 1111
Size: 60 4591
BsiEI CG_ry'CG
Cuts at: 104 864 2191 2237 3447 104
Size: 760 1327 46 1210 1308
Fragments arranged by size:
1327 1308 1210 760 46
BsiHKAI G_wGCw'C
Cuts at: 493 974 1663 2441 2631 3425 493
Size: 481 689 778 190 794 1719
Fragments arranged by size:
1719 794 778 689 481 190
BsmBI CGTCTCn'nnnn_
Cuts at: 679 679
Size: 4651
Bsp1286I G_dGCh'C
Cuts at: 173 273 493 734 958 974 1098 1159 1663
Size: 100 220 241 224 16 124 61 504
Cuts at: 1663 2274 2367 2441 2631 2693 3425 173
Size: 611 93 74 190 62 732 1399
Fragments arranged by size:
1399 732 611 504 241 224 220 190
124 100 93 74 62 61 16
BspEI T'CCGG_A
Cuts at: 677 3871 677
Size: 3194 1457
BspHI T'CATG_A
Cuts at: 3827 3827
Size: 4651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1133 1145 1699 1913 2215 2596 1133
Size: 12 554 214 302 381 3188
Fragments arranged by size:
3188 554 381 302 214 12
BsrBI CCG'CTC
Cuts at: 50 2941 2995 4481 4646 50
Size: 2891 54 1486 165 55
Fragments arranged by size:
2891 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2561 2561
Size: 4651
BsrFI r'CCGG_y
Cuts at: 863 2647 2828 3039 863
Size: 1784 181 211 2475
Fragments arranged by size:
2475 1784 211 181
BsrGI T'GTAC_A
Cuts at: 243 243
Size: 4651
BssSI C'ACGA_G
Cuts at: 2920 3280 2920
Size: 360 4291
BstAPI GCAn_nnn'nTGC
Cuts at: 286 2026 286
Size: 1740 2911
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4651
BstXI CCAn_nnnn'nTGG
Cuts at: 618 618
Size: 4651
BstYI r'GATC_y
Cuts at: 1 497 623 1430 1674 2141 2164 2499 2745
Size: 496 126 807 244 467 23 335 246
Cuts at: 2745 3759 3845 3857 4203 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 807 496 467 449 346 335 246
244 126 86 23 12
BtgI C'CryG_G
Cuts at: 741 741
Size: 4651
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2599 2780 2943 2599
Size: 181 163 4307
Fragments arranged by size:
4307 181 163
BtsI GCAGTG_nn'
Cuts at: 478 675 756 950 1892 478
Size: 197 81 194 942 3237
Fragments arranged by size:
3237 942 197 194 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1332 1367 1332
Size: 35 4616
DraI TTT'AAA
Cuts at: 1648 1648
Size: 4651
DraIII CAC_nnn'GTG
Cuts at: 1620 1620
Size: 4651
DrdI GACnn_nn'nnGTC
Cuts at: 2355 3215 2355
Size: 860 3791
EaeI y'GGCC_r
Cuts at: 2234 2408 2799 2826 4173 2234
Size: 174 391 27 1347 2712
Fragments arranged by size:
2712 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2234 2234
Size: 4651
EarI CTCTTCn'nnn_
Cuts at: 557 2672 2882 557
Size: 2115 210 2326
Fragments arranged by size:
2326 2115 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 202 2857 3169 3315 4461 202
Size: 2655 312 146 1146 392
Fragments arranged by size:
2655 1146 392 312 146
EcoICRI GAG'CTC
Cuts at: 1661 1661
Size: 4651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 448 581 958 1402 1523 2474 2906 3655
Size: 3 133 377 444 121 951 432 749
Cuts at: 3655 3923 445
Size: 268 1173
Fragments arranged by size:
1173 951 749 444 432 377 268 133
121 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1150 1150
Size: 4651
EcoO109I rG'GnC_Cy
Cuts at: 1143 1788 1143
Size: 645 4006
EcoRI G'AATT_C
Cuts at: 1653 1653
Size: 4651
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 301 1312 2668 301
Size: 1011 1356 2284
Fragments arranged by size:
2284 1356 1011
FspI TGC'GCA
Cuts at: 709 2430 709
Size: 1721 2930
HaeII r_GCGC'y
Cuts at: 2083 2331 3355 4334 2083
Size: 248 1024 979 2400
Fragments arranged by size:
2400 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 348 380 526 558 615 647 871 903 2491
Size: 32 146 32 57 32 224 32 1588
Cuts at: 2491 2521 2523 2553 348
Size: 30 2 30 2446
Fragments arranged by size:
2446 1588 224 146 57 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 540 1688 540
Size: 1148 3503
HindIII A'AGCT_T
Cuts at: 701 1028 701
Size: 327 4324
KasI G'GCGC_C
Cuts at: 2079 2327 2079
Size: 248 4403
KpnI G_GTAC'C
Cuts at: 909 1236 1463 1669 909
Size: 327 227 206 3891
Fragments arranged by size:
3891 327 227 206
MluI A'CGCG_T
Cuts at: 4084 4084
Size: 4651
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 510 884 902 3322 3506 510
Size: 374 18 2420 184 1655
Fragments arranged by size:
2420 1655 374 184 18
MscI TGG'CCA
Cuts at: 2410 2410
Size: 4651
MslI CAynn'nnrTG
Cuts at: 835 916 1406 2638 2765 835
Size: 81 490 1232 127 2721
Fragments arranged by size:
2721 1232 490 127 81
MspA1I CmG'CkG
Cuts at: 423 743 872 1424 1760 2074 2434 3449 3694
Size: 320 129 552 336 314 360 1015 245
Cuts at: 3694 423
Size: 1380
Fragments arranged by size:
1380 1015 552 360 336 320 314 245
129
NaeI GCC'GGC
Cuts at: 2830 2830
Size: 4651
NarI GG'CG_CC
Cuts at: 2080 2328 2080
Size: 248 4403
NdeI CA'TA_TG
Cuts at: 1319 1319
Size: 4651
NgoMIV G'CCGG_C
Cuts at: 2828 2828
Size: 4651
NruI TCG'CGA
Cuts at: 3012 3012
Size: 4651
NspI r_CATG'y
Cuts at: 1311 1702 2202 3111 1311
Size: 391 500 909 2851
Fragments arranged by size:
2851 909 500 391
PasI CC'CwG_GG
Cuts at: 891 891
Size: 4651
PciI A'CATG_T
Cuts at: 3107 3107
Size: 4651
PfoI T'CCnGG_A
Cuts at: 308 1600 308
Size: 1292 3359
PmeI GTTT'AAAC
Cuts at: 1648 1648
Size: 4651
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3790 3822 3790
Size: 32 4619
PstI C_TGCA'G
Cuts at: 221 453 1142 1696 2381 221
Size: 232 689 554 685 2491
Fragments arranged by size:
2491 689 685 554 232
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4651
PvuII CAG'CTG
Cuts at: 423 872 2074 2434 423
Size: 449 1202 360 2640
Fragments arranged by size:
2640 1202 449 360
RsrII CG'GwC_CG
Cuts at: 2844 2844
Size: 4651
SacI G_AGCT'C
Cuts at: 1663 1663
Size: 4651
SacII CC_GC'GG
Cuts at: 744 744
Size: 4651
SalI G'TCGA_C
Cuts at: 1686 1686
Size: 4651
SapI GCTCTTCn'nnn_
Cuts at: 2672 2882 2672
Size: 210 4441
SbfI CC_TGCA'GG
Cuts at: 221 1142 1696 221
Size: 921 554 3176
Fragments arranged by size:
3176 921 554
ScaI AGT'ACT
Cuts at: 4168 4168
Size: 4651
SfcI C'TryA_G
Cuts at: 33 217 449 1138 1692 2377 3372 3563 33
Size: 184 232 689 554 685 995 191 1121
Fragments arranged by size:
1121 995 689 685 554 232 191 184
SfoI GGC'GCC
Cuts at: 2081 2329 2081
Size: 248 4403
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4651
SmaI CCC'GGG
Cuts at: 1188 1207 1671 1188
Size: 19 464 4168
Fragments arranged by size:
4168 464 19
SmlI C'TyrA_G
Cuts at: 1804 2719 3213 3475 1804
Size: 915 494 262 2980
Fragments arranged by size:
2980 915 494 262
SnaBI TAC'GTA
Cuts at: 1641 1641
Size: 4651
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4651
SphI G_CATG'C
Cuts at: 1702 2202 1702
Size: 500 4151
StyI C'CwwG_G
Cuts at: 1455 1783 2137 1455
Size: 328 354 3969
Fragments arranged by size:
3969 354 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 883 1505 2232 2365 2824 3035 883
Size: 622 727 133 459 211 2499
Fragments arranged by size:
2499 727 622 459 211 133
TatI w'GTAC_w
Cuts at: 176 243 1361 4166 176
Size: 67 1118 2805 661
Fragments arranged by size:
2805 1118 661 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 216 248 970 1002 4168 4200 216
Size: 32 722 32 3166 32 667
Fragments arranged by size:
3166 722 667 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2446 2446
Size: 4651
XbaI T'CTAG_A
Cuts at: 1680 1680
Size: 4651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2144 2144
Size: 4651
XmaI C'CCGG_G
Cuts at: 1186 1205 1669 1186
Size: 19 464 4168
Fragments arranged by size:
4168 464 19
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NruI
NspI PasI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BssHII BstEII BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HpaI MfeI NcoI NheI NotI NsiI
PacI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI ZraI