Restriction map for
pF1KSDA1994
(Circular) (Six-base) MAPSORT of: pF1KSDA1994.seq Check: 4577 from: 1 to: 6358
pF1KSDA1994 6358 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 990 2433 990
Size: 1443 4915
AccI GT'mk_AC
Cuts at: 1728 3394 1728
Size: 1666 4692
Acc65I G'GTAC_C
Cuts at: 259 3372 259
Size: 3113 3245
AclI AA'CG_TT
Cuts at: 6198 6198
Size: 6358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1795 1874 2069 2363 3226 4181 4613 5362
Size: 1494 79 195 294 863 955 432 749
Cuts at: 5362 5630 301
Size: 268 1029
Fragments arranged by size:
1494 1029 955 863 749 432 294 268
195 79
AfeI AGC'GCT
Cuts at: 180 180
Size: 6358
AflIII A'CryG_T
Cuts at: 3708 4814 5791 3708
Size: 1106 977 4275
Fragments arranged by size:
4275 1106 977
AgeI A'CCGG_T
Cuts at: 4746 4746
Size: 6358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2597 2597
Size: 6358
AlwNI CAG_nnn'CTG
Cuts at: 2287 2971 3661 5230 2287
Size: 684 690 1569 3415
Fragments arranged by size:
3415 1569 690 684
ApaLI G'TGCA_C
Cuts at: 5128 5128
Size: 6358
ApoI r'AATT_y
Cuts at: 1038 2772 3174 3360 5711 5967 1038
Size: 1734 402 186 2351 256 1429
Fragments arranged by size:
2351 1734 1429 402 256 186
AseI AT'TA_AT
Cuts at: 20 20
Size: 6358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6358
AvaI C'yCGr_G
Cuts at: 3376 3376
Size: 6358
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 251 284 251
Size: 33 6325
BamHI G'GATC_C
Cuts at: 3381 3381
Size: 6358
BanI G'GyrC_C
Cuts at: 259 812 1986 3292 3372 3786 4034 4069 259
Size: 553 1174 1306 80 414 248 35 2548
Fragments arranged by size:
2548 1306 1174 553 414 248 80 35
BanII G_rGCy'C
Cuts at: 629 1379 1599 2882 3040 3331 3370 4400 629
Size: 750 220 1283 158 291 39 1030 2587
Fragments arranged by size:
2587 1283 1030 750 291 220 158 39
BbeI G_GCGC'C
Cuts at: 3790 4038 3790
Size: 248 6110
BbsI GAAGACnn'nnnn_
Cuts at: 1801 3214 1801
Size: 1413 4945
BbvCI CC'TCA_GC
Cuts at: 716 716
Size: 6358
BciVI GTATCCnnnnn_n'
Cuts at: 1864 2209 4254 5017 6358 1864
Size: 345 2045 763 1341 1864
Fragments arranged by size:
2045 1864 1341 763 345
BclI T'GATC_A
Cuts at: 755 3876 755
Size: 3121 3237
BglII A'GATC_T
Cuts at: 485 567 1754 3871 5910 485
Size: 82 1187 2117 2039 933
Fragments arranged by size:
2117 2039 1187 933 82
BlpI GC'TnA_GC
Cuts at: 398 2186 3468 398
Size: 1788 1282 3288
Fragments arranged by size:
3288 1788 1282
Bme1580I G_kGCm'C
Cuts at: 815 1989 3295 3981 4074 5132 815
Size: 1174 1306 686 93 1058 2041
Fragments arranged by size:
2041 1306 1174 1058 686 93
BmrI ACTGGGnnnn_n'
Cuts at: 889 1301 2135 2444 2805 3231 3568 3649 3984
Size: 412 834 309 361 426 337 81 335
Cuts at: 3984 6130 889
Size: 2146 1117
Fragments arranged by size:
2146 1117 834 426 412 361 337 335
309 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1760 3057 4723 1760
Size: 1297 1666 3395
Fragments arranged by size:
3395 1666 1297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1205 1946 3205 1205
Size: 741 1259 4358
Fragments arranged by size:
4358 1259 741
Bpu10I CC'TnA_GC
Cuts at: 716 2834 3224 3330 716
Size: 2118 390 106 3744
Fragments arranged by size:
3744 2118 390 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 1236 1763 2540 3532 4411 4905 5203 873
Size: 363 527 777 992 879 494 298 2028
Fragments arranged by size:
2028 992 879 777 527 494 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 643 6062 643
Size: 5419 939
BsaAI yAC'GTr
Cuts at: 3348 3709 3348
Size: 361 5997
BsaBI GATnn'nnATC
Cuts at: 6 3870 5909 6
Size: 3864 2039 455
Fragments arranged by size:
3864 2039 455
BsaHI Gr'CG_yC
Cuts at: 266 3787 4035 4739 6235 266
Size: 3521 248 704 1496 389
Fragments arranged by size:
3521 1496 704 389 248
BsaWI w'CCGG_w
Cuts at: 2131 3545 4066 4746 5020 5167 5578 2131
Size: 1414 521 680 274 147 411 2911
Fragments arranged by size:
2911 1414 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 767 797 1643 1673 2451 2481 2508 2538 767
Size: 30 846 30 778 30 27 30 4587
Fragments arranged by size:
4587 846 778 30 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 630 1949 2390 3030 630
Size: 1319 441 640 3958
Fragments arranged by size:
3958 1319 640 441
BseYI C'CCAG_C
Cuts at: 1934 2229 2528 3179 3248 3281 3311 3781 4539
Size: 295 299 651 69 33 30 470 758
Cuts at: 4539 5118 5614 1934
Size: 579 496 2678
Fragments arranged by size:
2678 758 651 579 496 470 299 295
69 33 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1633 2233 2371 2891 1633
Size: 600 138 520 5100
Fragments arranged by size:
5100 600 520 138
BsiEI CG_ry'CG
Cuts at: 104 158 3898 3944 5154 104
Size: 54 3740 46 1210 1308
Fragments arranged by size:
3740 1308 1210 54 46
BsiHKAI G_wGCw'C
Cuts at: 1011 1519 2400 2469 3040 3370 4148 4338 5132
Size: 508 881 69 571 330 778 190 794
Cuts at: 5132 1011
Size: 2237
Fragments arranged by size:
2237 881 794 778 571 508 330 190
69
BsmI GAATG_Cn'
Cuts at: 771 2660 771
Size: 1889 4469
Bsp1286I G_dGCh'C
Cuts at: 629 815 1011 1379 1519 1599 1989 2400 2469
Size: 186 196 368 140 80 390 411 69
Cuts at: 2469 2882 3040 3295 3331 3370 3981 4074 4148
Size: 413 158 255 36 39 611 93 74
Cuts at: 4148 4338 4400 5132 629
Size: 190 62 732 1855
Fragments arranged by size:
1855 732 611 413 411 390 368 255
196 190 186 158 140 93 80 74
69 62 39 36
BspEI T'CCGG_A
Cuts at: 2131 5578 2131
Size: 3447 2911
BspHI T'CATG_A
Cuts at: 5534 5534
Size: 6358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 247 990 1443 2247 2433 3406 3620 3922 4303
Size: 743 453 804 186 973 214 302 381
Cuts at: 4303 247
Size: 2302
Fragments arranged by size:
2302 973 804 743 453 381 302 214
186
BsrBI CCG'CTC
Cuts at: 50 4648 4702 6188 6353 50
Size: 4598 54 1486 165 55
Fragments arranged by size:
4598 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 209 1236 2129 4268 209
Size: 1027 893 2139 2299
Fragments arranged by size:
2299 2139 1027 893
BsrFI r'CCGG_y
Cuts at: 262 4354 4535 4746 262
Size: 4092 181 211 1874
Fragments arranged by size:
4092 1874 211 181
BsrGI T'GTAC_A
Cuts at: 2094 2094
Size: 6358
BssSI C'ACGA_G
Cuts at: 4627 4987 4627
Size: 360 5998
BstAPI GCAn_nnn'nTGC
Cuts at: 2239 2287 2971 3733 2239
Size: 48 684 762 4864
Fragments arranged by size:
4864 762 684 48
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6358
BstEII G'GTnAC_C
Cuts at: 829 829
Size: 6358
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2263
Size: 6358
BstYI r'GATC_y
Cuts at: 1 485 567 584 1754 3090 3381 3848 3871
Size: 484 82 17 1170 1336 291 467 23
Cuts at: 3871 4206 4452 5466 5552 5564 5910 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1336 1170 1014 484 467 449 346 335
291 246 86 82 23 17 12
BstZ17I GTA'TAC
Cuts at: 1729 1729
Size: 6358
BtgI C'CryG_G
Cuts at: 152 152
Size: 6358
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1847 3305 4306 4487 4650 1847
Size: 1458 1001 181 163 3555
Fragments arranged by size:
3555 1458 1001 181 163
BtsI GCAGTG_nn'
Cuts at: 1269 2288 2354 3599 1269
Size: 1019 66 1245 4028
Fragments arranged by size:
4028 1245 1019 66
DraI TTT'AAA
Cuts at: 1383 3355 1383
Size: 1972 4386
DrdI GACnn_nn'nnGTC
Cuts at: 4062 4922 4062
Size: 860 5498
EaeI y'GGCC_r
Cuts at: 155 857 2265 3215 3941 4115 4506 4533 5880
Size: 702 1408 950 726 174 391 27 1347
Cuts at: 5880 155
Size: 633
Fragments arranged by size:
1408 1347 950 726 702 633 391 174
27
EagI C'GGCC_G
Cuts at: 155 3941 155
Size: 3786 2572
EarI CTCTTCn'nnn_
Cuts at: 579 641 4379 4589 579
Size: 62 3738 210 2348
Fragments arranged by size:
3738 2348 210 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 290 353 443 4564 4876 5022 6168 290
Size: 63 90 4121 312 146 1146 480
Fragments arranged by size:
4121 1146 480 312 146 90 63
EcoICRI GAG'CTC
Cuts at: 3038 3368 3038
Size: 330 6028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1205 1795 1874 1946 2069 2363 3205 3226
Size: 904 590 79 72 123 294 842 21
Cuts at: 3226 4181 4613 5362 5630 301
Size: 955 432 749 268 1029
Fragments arranged by size:
1029 955 904 842 749 590 432 294
268 123 79 72 21
EcoNI CCTnn'n_nnAGG
Cuts at: 391 391
Size: 6358
EcoO109I rG'GnC_Cy
Cuts at: 3138 3495 3138
Size: 357 6001
EcoRI G'AATT_C
Cuts at: 3360 3360
Size: 6358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4375 4375
Size: 6358
FspI TGC'GCA
Cuts at: 4137 4137
Size: 6358
HaeII r_GCGC'y
Cuts at: 182 239 3790 4038 5062 6041 182
Size: 57 3551 248 1024 979 499
Fragments arranged by size:
3551 1024 979 499 248 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2061 2093 2430 2462 3022 3054 4198 4228 4230
Size: 32 337 32 560 32 1144 30 2
Cuts at: 4230 4260 2061
Size: 30 4159
Fragments arranged by size:
4159 1144 560 337 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1074 1332 2516 2746 3395 1074
Size: 258 1184 230 649 4037
Fragments arranged by size:
4037 1184 649 258 230
HpaI GTT'AAC
Cuts at: 1074 1332 2516 1074
Size: 258 1184 4916
Fragments arranged by size:
4916 1184 258
KasI G'GCGC_C
Cuts at: 3786 4034 3786
Size: 248 6110
KpnI G_GTAC'C
Cuts at: 263 3376 263
Size: 3113 3245
MfeI C'AATT_G
Cuts at: 2485 2840 2485
Size: 355 6003
MluI A'CGCG_T
Cuts at: 5791 5791
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 846 1701 5029 5213 846
Size: 855 3328 184 1991
Fragments arranged by size:
3328 1991 855 184
MscI TGG'CCA
Cuts at: 859 2267 3217 4117 859
Size: 1408 950 900 3100
Fragments arranged by size:
3100 1408 950 900
MslI CAynn'nnrTG
Cuts at: 4345 4472 4345
Size: 127 6231
MspA1I CmG'CkG
Cuts at: 154 166 224 402 1279 2205 2875 3003 3183
Size: 12 58 178 877 926 670 128 180
Cuts at: 3183 3252 3467 3781 4141 5156 5401 154
Size: 69 215 314 360 1015 245 1111
Fragments arranged by size:
1111 1015 926 877 670 360 314 245
215 180 178 128 69 58 12
NaeI GCC'GGC
Cuts at: 4537 4537
Size: 6358
NarI GG'CG_CC
Cuts at: 3787 4035 3787
Size: 248 6110
NgoMIV G'CCGG_C
Cuts at: 4535 4535
Size: 6358
NotI GC'GGCC_GC
Cuts at: 155 155
Size: 6358
NruI TCG'CGA
Cuts at: 4719 4719
Size: 6358
NspI r_CATG'y
Cuts at: 3409 3909 4818 3409
Size: 500 909 4949
Fragments arranged by size:
4949 909 500
PciI A'CATG_T
Cuts at: 4814 4814
Size: 6358
PflMI CCAn_nnn'nTGG
Cuts at: 1683 1683
Size: 6358
PfoI T'CCnGG_A
Cuts at: 665 665
Size: 6358
PmeI GTTT'AAAC
Cuts at: 3355 3355
Size: 6358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5497 5529 5497
Size: 32 6326
PpuMI rG'GwC_Cy
Cuts at: 3138 3138
Size: 6358
PstI C_TGCA'G
Cuts at: 171 336 407 1284 2151 2244 2349 2586 3257
Size: 165 71 877 867 93 105 237 671
Cuts at: 3257 3403 4088 171
Size: 146 685 2441
Fragments arranged by size:
2441 877 867 685 671 237 165 146
105 93 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6358
PvuII CAG'CTG
Cuts at: 402 1279 2205 3183 3252 3781 4141 402
Size: 877 926 978 69 529 360 2619
Fragments arranged by size:
2619 978 926 877 529 360 69
RsrII CG'GwC_CG
Cuts at: 4551 4551
Size: 6358
SacI G_AGCT'C
Cuts at: 3040 3370 3040
Size: 330 6028
SacII CC_GC'GG
Cuts at: 155 155
Size: 6358
SalI G'TCGA_C
Cuts at: 3393 3393
Size: 6358
SapI GCTCTTCn'nnn_
Cuts at: 4379 4589 4379
Size: 210 6148
SbfI CC_TGCA'GG
Cuts at: 2244 3403 2244
Size: 1159 5199
ScaI AGT'ACT
Cuts at: 1017 5875 1017
Size: 4858 1500
SfcI C'TryA_G
Cuts at: 33 167 332 403 1019 1280 1439 2147 2240
Size: 134 165 71 616 261 159 708 93
Cuts at: 2240 2345 2582 3253 3399 4084 5079 5270 33
Size: 105 237 671 146 685 995 191 1121
Fragments arranged by size:
1121 995 708 685 671 616 261 237
191 165 159 146 134 105 93 71
SfoI GGC'GCC
Cuts at: 3788 4036 3788
Size: 248 6110
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6358
SmaI CCC'GGG
Cuts at: 3378 3378
Size: 6358
SmlI C'TyrA_G
Cuts at: 888 1215 1778 2555 3511 4426 4920 5182 888
Size: 327 563 777 956 915 494 262 2064
Fragments arranged by size:
2064 956 915 777 563 494 327 262
SnaBI TAC'GTA
Cuts at: 3348 3348
Size: 6358
SpeI A'CTAG_T
Cuts at: 67 1054 1519 2461 67
Size: 987 465 942 3964
Fragments arranged by size:
3964 987 942 465
SphI G_CATG'C
Cuts at: 3409 3909 3409
Size: 500 5858
SspI AAT'ATT
Cuts at: 1152 2319 1152
Size: 1167 5191
StyI C'CwwG_G
Cuts at: 1122 1662 3490 3844 1122
Size: 540 1828 354 3636
Fragments arranged by size:
3636 1828 540 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3295 3939 4072 4531 4742 3295
Size: 644 133 459 211 4911
Fragments arranged by size:
4911 644 459 211 133
TatI w'GTAC_w
Cuts at: 1015 1057 1141 1764 2094 5873 1015
Size: 42 84 623 330 3779 1500
Fragments arranged by size:
3779 1500 623 330 84 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 767 799 2156 2183 2188 2215 5875 5907 767
Size: 32 1357 27 5 27 3660 32 1218
Fragments arranged by size:
3660 1357 1218 32 32 27 27 5
Tth111I GACn'n_nGTC
Cuts at: 4153 4153
Size: 6358
XbaI T'CTAG_A
Cuts at: 901 3387 901
Size: 2486 3872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 823 1920 3256 3851 823
Size: 1097 1336 595 3330
Fragments arranged by size:
3330 1336 1097 595
XmaI C'CCGG_G
Cuts at: 3376 3376
Size: 6358
XmnI GAAnn'nnTTC
Cuts at: 2565 2658 2565
Size: 93 6265
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NruI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
NcoI NdeI NheI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SrfI StuI SwaI XhoI ZraI