Restriction map for
pF1KSDA2003
(Circular) (Six-base) MAPSORT of: pF1KSDA2003.seq Check: 7758 from: 1 to: 4448
pF1KSDA2003 4448 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 403 403
Size: 4448
AccI GT'mk_AC
Cuts at: 1486 1486
Size: 4448
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 2705 3452 3720 2273
Size: 432 747 268 3001
Fragments arranged by size:
3001 747 432 268
AflIII A'CryG_T
Cuts at: 1800 2904 3881 1800
Size: 1104 977 2367
Fragments arranged by size:
2367 1104 977
AgeI A'CCGG_T
Cuts at: 2836 2836
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 1753 3320 1753
Size: 1567 2881
ApaLI G'TGCA_C
Cuts at: 1368 3218 1368
Size: 1850 2598
ApoI r'AATT_y
Cuts at: 1314 1424 1452 3801 4057 1314
Size: 110 28 2349 256 1705
Fragments arranged by size:
2349 1705 256 110 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 1468 1468
Size: 4448
BamHI G'GATC_C
Cuts at: 1015 1473 1015
Size: 458 3990
BanI G'GyrC_C
Cuts at: 1464 1878 2126 2161 1464
Size: 414 248 35 3751
Fragments arranged by size:
3751 414 248 35
BanII G_rGCy'C
Cuts at: 1462 2492 1462
Size: 1030 3418
BbeI G_GCGC'C
Cuts at: 1882 2130 1882
Size: 248 4200
BbsI GAAGACnn'nnnn_
Cuts at: 364 364
Size: 4448
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3107 4448 2346
Size: 761 1341 2346
Fragments arranged by size:
2346 1341 761
BclI T'GATC_A
Cuts at: 1968 1968
Size: 4448
BglII A'GATC_T
Cuts at: 1963 4000 1963
Size: 2037 2411
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 163 1372 2073 2166 3222 163
Size: 1209 701 93 1056 1389
Fragments arranged by size:
1389 1209 1056 701 93
BmrI ACTGGGnnnn_n'
Cuts at: 470 854 938 1190 1358 1660 1741 2076 4220
Size: 384 84 252 168 302 81 335 2144
Cuts at: 4220 470
Size: 698
Fragments arranged by size:
2144 698 384 335 302 252 168 84
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 698 1118 1218 1386 277
Size: 421 420 100 168 3339
Fragments arranged by size:
3339 421 420 168 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2503 2995 3293 1624
Size: 879 492 298 2779
Fragments arranged by size:
2779 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 4152 217
Size: 3935 513
BsaAI yAC'GTr
Cuts at: 1440 1801 1440
Size: 361 4087
BsaBI GATnn'nnATC
Cuts at: 6 290 1962 3999 6
Size: 284 1672 2037 455
Fragments arranged by size:
2037 1672 455 284
BsaHI Gr'CG_yC
Cuts at: 148 1879 2127 2829 4325 148
Size: 1731 248 702 1496 271
Fragments arranged by size:
1731 1496 702 271 248
BsaWI w'CCGG_w
Cuts at: 746 1637 2158 2836 3110 3257 3668 746
Size: 891 521 678 274 147 411 1526
Fragments arranged by size:
1526 891 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1873 2631 3208 3704 1873
Size: 758 577 496 2617
Fragments arranged by size:
2617 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 884 885 969 1053 1136 1137 1221 1305
Size: 536 1 84 84 83 1 84 84
Cuts at: 1305 348
Size: 3491
Fragments arranged by size:
3491 536 84 84 84 84 83 1
1
BsiEI CG_ry'CG
Cuts at: 104 1990 2036 3244 104
Size: 1886 46 1208 1308
Fragments arranged by size:
1886 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1372 1462 2240 2430 3222 1372
Size: 90 778 190 792 2598
Fragments arranged by size:
2598 792 778 190 90
BsmI GAATG_Cn'
Cuts at: 547 547
Size: 4448
Bsp1286I G_dGCh'C
Cuts at: 163 1372 1462 2073 2166 2240 2430 2492 3222
Size: 1209 90 611 93 74 190 62 730
Cuts at: 3222 163
Size: 1389
Fragments arranged by size:
1389 1209 730 611 190 93 90 74
62
BspEI T'CCGG_A
Cuts at: 3668 3668
Size: 4448
BspHI T'CATG_A
Cuts at: 774 3624 774
Size: 2850 1598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 370 403 1498 1712 2014 2395 370
Size: 33 1095 214 302 381 2423
Fragments arranged by size:
2423 1095 381 302 214 33
BsrBI CCG'CTC
Cuts at: 50 2740 2794 4278 4443 50
Size: 2690 54 1484 165 55
Fragments arranged by size:
2690 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 339 2360 339
Size: 2021 2427
BsrFI r'CCGG_y
Cuts at: 2446 2627 2836 2446
Size: 181 209 4058
Fragments arranged by size:
4058 209 181
BssSI C'ACGA_G
Cuts at: 2719 3077 2719
Size: 358 4090
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 110 2809 110
Size: 2699 1749
BstYI r'GATC_y
Cuts at: 1 1015 1473 1940 1963 2298 2544 3556 3642
Size: 1014 458 467 23 335 246 1012 86
Cuts at: 3642 3654 4000 1
Size: 12 346 449
Fragments arranged by size:
1014 1012 467 458 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2398 2579 2742 2398
Size: 181 163 4104
Fragments arranged by size:
4104 181 163
BtsI GCAGTG_nn'
Cuts at: 621 758 873 957 1209 1293 1691 621
Size: 137 115 84 252 84 398 3378
Fragments arranged by size:
3378 398 252 137 115 84 84
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3012 2154
Size: 858 3590
EaeI y'GGCC_r
Cuts at: 134 182 422 2033 2207 2598 2625 3970 134
Size: 48 240 1611 174 391 27 1345 612
Fragments arranged by size:
1611 1345 612 391 240 174 48 27
EagI C'GGCC_G
Cuts at: 2033 2033
Size: 4448
EarI CTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2656 2966 3112 4258 2656
Size: 310 146 1146 2846
Fragments arranged by size:
2846 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1460 1460
Size: 4448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 698 1118 1218 1386 2273 2705 3452 3720
Size: 421 420 100 168 887 432 747 268
Cuts at: 3720 277
Size: 1005
Fragments arranged by size:
1005 887 747 432 421 420 268 168
100
EcoNI CCTnn'n_nnAGG
Cuts at: 220 220
Size: 4448
EcoO109I rG'GnC_Cy
Cuts at: 1587 1587
Size: 4448
EcoRI G'AATT_C
Cuts at: 1424 1452 1424
Size: 28 4420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 1882 2130 3152 4131 1882
Size: 248 1022 979 2199
Fragments arranged by size:
2199 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 138 170 2290 2320 2322 2352 138
Size: 32 2120 30 2 30 2234
Fragments arranged by size:
2234 2120 32 30 30 2
HincII GTy'rAC
Cuts at: 1487 1487
Size: 4448
KasI G'GCGC_C
Cuts at: 1878 2126 1878
Size: 248 4200
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3119 3303 3119
Size: 184 4264
MscI TGG'CCA
Cuts at: 424 2209 424
Size: 1785 2663
MslI CAynn'nnrTG
Cuts at: 779 861 2437 2564 779
Size: 82 1576 127 2663
Fragments arranged by size:
2663 1576 127 82
MspA1I CmG'CkG
Cuts at: 133 554 1559 1873 2233 3246 3491 133
Size: 421 1005 314 360 1013 245 1090
Fragments arranged by size:
1090 1013 1005 421 360 314 245
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 4448
NarI GG'CG_CC
Cuts at: 1879 2127 1879
Size: 248 4200
NcoI C'CATG_G
Cuts at: 125 125
Size: 4448
NdeI CA'TA_TG
Cuts at: 610 858 610
Size: 248 4200
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 4448
NspI r_CATG'y
Cuts at: 1501 2001 2908 1501
Size: 500 907 3041
Fragments arranged by size:
3041 907 500
PciI A'CATG_T
Cuts at: 2904 2904
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4416
PsiI TTA'TAA
Cuts at: 1112 1112
Size: 4448
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 576 608 576
Size: 32 4416
PstI C_TGCA'G
Cuts at: 400 765 1495 2180 400
Size: 365 730 685 2668
Fragments arranged by size:
2668 730 685 365
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 1873 2233 1873
Size: 360 4088
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 1462 1462
Size: 4448
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 396 593 645 761 1227 1491 2176 3169
Size: 363 197 52 116 466 264 685 993
Cuts at: 3169 3360 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 466 363 264 197 191
116 52
SfoI GGC'GCC
Cuts at: 1880 2128 1880
Size: 248 4200
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 1470 1470
Size: 4448
SmlI C'TyrA_G
Cuts at: 1603 2518 3010 3272 1603
Size: 915 492 262 2779
Fragments arranged by size:
2779 915 492 262
SnaBI TAC'GTA
Cuts at: 1440 1440
Size: 4448
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
StuI AGG'CCT
Cuts at: 939 1023 1107 1191 939
Size: 84 84 84 4196
Fragments arranged by size:
4196 84 84 84
StyI C'CwwG_G
Cuts at: 125 309 1582 1936 125
Size: 184 1273 354 2637
Fragments arranged by size:
2637 1273 354 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2031 2164 2623 2832 2031
Size: 133 459 209 3647
Fragments arranged by size:
3647 459 209 133
TatI w'GTAC_w
Cuts at: 3963 3963
Size: 4448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1000 1032 3965 3997 1000
Size: 32 2933 32 1451
Fragments arranged by size:
2933 1451 32 32
Tth111I GACn'n_nGTC
Cuts at: 2245 2245
Size: 4448
XbaI T'CTAG_A
Cuts at: 282 1479 282
Size: 1197 3251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1943 1943
Size: 4448
XmaI C'CCGG_G
Cuts at: 1468 1468
Size: 4448
XmnI GAAnn'nnTTC
Cuts at: 415 415
Size: 4448
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PmeI PpiI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SrfI
SspI SwaI XhoI ZraI