Restriction map for
pF1KSDA2008
(Circular) (Six-base) MAPSORT of: pF1KSDA2008.seq Check: 7015 from: 1 to: 5513
pF1KSDA2008 5513 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2011 2242 2301 2011
Size: 231 59 5223
Fragments arranged by size:
5223 231 59
AatII G_ACGT'C
Cuts at: 244 244
Size: 5513
AccI GT'mk_AC
Cuts at: 1916 1976 2551 1916
Size: 60 575 4878
Fragments arranged by size:
4878 575 60
Acc65I G'GTAC_C
Cuts at: 1161 2529 1161
Size: 1368 4145
AclI AA'CG_TT
Cuts at: 5353 5353
Size: 5513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1156 1779 2394 3338 3770 4517 4785 1063
Size: 93 623 615 944 432 747 268 1791
Fragments arranged by size:
1791 944 747 623 615 432 268 93
AflIII A'CryG_T
Cuts at: 1022 1904 2865 3969 4946 1022
Size: 882 961 1104 977 1589
Fragments arranged by size:
1589 1104 977 961 882
AgeI A'CCGG_T
Cuts at: 1280 3901 1280
Size: 2621 2892
AleI CACnn'nnGTG
Cuts at: 304 304
Size: 5513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 430 1798 430
Size: 1368 4145
AlwNI CAG_nnn'CTG
Cuts at: 540 939 951 984 1692 1695 2071 2818 4385
Size: 399 12 33 708 3 376 747 1567
Cuts at: 4385 540
Size: 1668
Fragments arranged by size:
1668 1567 747 708 399 376 33 12
3
ApaI G_GGCC'C
Cuts at: 319 1055 1744 319
Size: 736 689 4088
Fragments arranged by size:
4088 736 689
ApaLI G'TGCA_C
Cuts at: 4283 4283
Size: 5513
ApoI r'AATT_y
Cuts at: 2517 4866 5122 2517
Size: 2349 256 2908
Fragments arranged by size:
2908 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5513
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
AvaI C'yCGr_G
Cuts at: 319 1034 1633 2134 2533 319
Size: 715 599 501 399 3299
Fragments arranged by size:
3299 715 599 501 399
AvrII C'CTAG_G
Cuts at: 1056 1056
Size: 5513
BamHI G'GATC_C
Cuts at: 485 2024 2538 485
Size: 1539 514 3460
Fragments arranged by size:
3460 1539 514
BanI G'GyrC_C
Cuts at: 269 600 700 789 1161 1283 1603 1638 2313
Size: 331 100 89 372 122 320 35 675
Cuts at: 2313 2529 2943 3191 3226 269
Size: 216 414 248 35 2556
Fragments arranged by size:
2556 675 414 372 331 320 248 216
122 100 89 35 35
BanII G_rGCy'C
Cuts at: 319 1055 1463 1744 2064 2104 2145 2264 2527
Size: 736 408 281 320 40 41 119 263
Cuts at: 2527 3557 319
Size: 1030 2275
Fragments arranged by size:
2275 1030 736 408 320 281 263 119
41 40
BbeI G_GCGC'C
Cuts at: 273 2947 3195 273
Size: 2674 248 2591
Fragments arranged by size:
2674 2591 248
BciVI GTATCCnnnnn_n'
Cuts at: 3411 4172 5513 3411
Size: 761 1341 3411
Fragments arranged by size:
3411 1341 761
BclI T'GATC_A
Cuts at: 128 3033 128
Size: 2905 2608
BglI GCCn_nnn'nGGC
Cuts at: 787 919 1954 2194 2203 787
Size: 132 1035 240 9 4097
Fragments arranged by size:
4097 1035 240 132 9
BglII A'GATC_T
Cuts at: 3028 5065 3028
Size: 2037 3476
BlpI GC'TnA_GC
Cuts at: 2625 2625
Size: 5513
Bme1580I G_kGCm'C
Cuts at: 319 657 792 1055 1744 2318 3138 3231 4287
Size: 338 135 263 689 574 820 93 1056
Cuts at: 4287 319
Size: 1545
Fragments arranged by size:
1545 1056 820 689 574 338 263 135
93
BmgBI CAC'GTC
Cuts at: 1023 1023
Size: 5513
BmrI ACTGGGnnnn_n'
Cuts at: 599 909 1380 2725 2806 3141 5285 599
Size: 310 471 1345 81 335 2144 827
Fragments arranged by size:
2144 1345 827 471 335 310 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1878 3880 1878
Size: 2002 3511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 418 783 1534 1774 1833 2038 2048 379
Size: 39 365 751 240 59 205 10 3844
Fragments arranged by size:
3844 751 365 240 205 59 39 10
Bpu10I CC'TnA_GC
Cuts at: 630 630
Size: 5513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2287 2689 3568 4060 4358 2287
Size: 402 879 492 298 3442
Fragments arranged by size:
3442 879 492 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 177 5217 177
Size: 5040 473
BsaAI yAC'GTr
Cuts at: 1905 2505 2866 1905
Size: 600 361 4552
Fragments arranged by size:
4552 600 361
BsaBI GATnn'nnATC
Cuts at: 6 3027 5064 6
Size: 3021 2037 455
Fragments arranged by size:
3021 2037 455
BsaHI Gr'CG_yC
Cuts at: 241 270 332 2294 2944 3192 3894 5390 241
Size: 29 62 1962 650 248 702 1496 364
Fragments arranged by size:
1962 1496 702 650 364 248 62 29
BsaWI w'CCGG_w
Cuts at: 1280 1361 2702 3223 3901 4175 4322 4733 1280
Size: 81 1341 521 678 274 147 411 2060
Fragments arranged by size:
2060 1341 678 521 411 274 147 81
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1024 1351 1471 1948 1024
Size: 327 120 477 4589
Fragments arranged by size:
4589 477 327 120
BseYI C'CCAG_C
Cuts at: 932 951 1865 2366 2938 3696 4273 4769 932
Size: 19 914 501 572 758 577 496 1676
Fragments arranged by size:
1676 914 758 577 572 501 496 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 421 544 1076 1090 1336 2208 400
Size: 21 123 532 14 246 872 3705
Fragments arranged by size:
3705 872 532 246 123 21 14
BsiEI CG_ry'CG
Cuts at: 104 3055 3101 4309 104
Size: 2951 46 1208 1308
Fragments arranged by size:
2951 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 712 1463 2064 2527 3305 3495 4287 712
Size: 751 601 463 778 190 792 1938
Fragments arranged by size:
1938 792 778 751 601 463 190
BsmBI CGTCTCn'nnnn_
Cuts at: 248 513 1462 248
Size: 265 949 4299
Fragments arranged by size:
4299 949 265
Bsp1286I G_dGCh'C
Cuts at: 319 657 712 792 1055 1463 1744 2064 2104
Size: 338 55 80 263 408 281 320 40
Cuts at: 2104 2145 2264 2318 2527 3138 3231 3305 3495
Size: 41 119 54 209 611 93 74 190
Cuts at: 3495 3557 4287 319
Size: 62 730 1545
Fragments arranged by size:
1545 730 611 408 338 320 281 263
209 190 119 93 80 74 62 55
54 41 40
BspEI T'CCGG_A
Cuts at: 4733 4733
Size: 5513
BspHI T'CATG_A
Cuts at: 884 1403 4689 884
Size: 519 3286 1708
Fragments arranged by size:
3286 1708 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 362 443 1329 2011 2242 2301 2396 2563 2777
Size: 81 886 682 231 59 95 167 214
Cuts at: 2777 3079 3460 362
Size: 302 381 2415
Fragments arranged by size:
2415 886 682 381 302 231 214 167
95 81 59
BsrBI CCG'CTC
Cuts at: 50 483 1188 2022 3805 3859 5343 5508 50
Size: 433 705 834 1783 54 1484 165 55
Fragments arranged by size:
1783 1484 834 705 433 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3425 3425
Size: 5513
BsrFI r'CCGG_y
Cuts at: 424 469 581 1280 2419 2441 3511 3692 3901
Size: 45 112 699 1139 22 1070 181 209
Cuts at: 3901 424
Size: 2036
Fragments arranged by size:
2036 1139 1070 699 209 181 112 45
22
BssSI C'ACGA_G
Cuts at: 1333 1822 3784 4142 1333
Size: 489 1962 358 2704
Fragments arranged by size:
2704 1962 489 358
BstAPI GCAn_nnn'nTGC
Cuts at: 707 1695 2190 2890 707
Size: 988 495 700 3330
Fragments arranged by size:
3330 988 700 495
BstBI TT'CG_AA
Cuts at: 110 3874 110
Size: 3764 1749
BstEII G'GTnAC_C
Cuts at: 174 174
Size: 5513
BstXI CCAn_nnnn'nTGG
Cuts at: 1509 1509
Size: 5513
BstYI r'GATC_y
Cuts at: 1 485 536 980 1595 2024 2538 3005 3028
Size: 484 51 444 615 429 514 467 23
Cuts at: 3028 3363 3609 4621 4707 4719 5065 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 615 514 484 467 449 444 429
346 335 246 86 51 23 12
BtgI C'CryG_G
Cuts at: 327 1110 1230 1514 1599 1659 2244 327
Size: 783 120 284 85 60 585 3596
Fragments arranged by size:
3596 783 585 284 120 85 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 501 1746 3463 3644 3807 501
Size: 1245 1717 181 163 2207
Fragments arranged by size:
2207 1717 1245 181 163
BtsI GCAGTG_nn'
Cuts at: 414 1078 2756 414
Size: 664 1678 3171
Fragments arranged by size:
3171 1678 664
DraI TTT'AAA
Cuts at: 2512 2512
Size: 5513
DraIII CAC_nnn'GTG
Cuts at: 607 707 607
Size: 100 5413
DrdI GACnn_nn'nnGTC
Cuts at: 1011 3219 4077 1011
Size: 2208 858 2447
Fragments arranged by size:
2447 2208 858
EaeI y'GGCC_r
Cuts at: 389 1511 2204 2373 3098 3272 3663 3690 5035
Size: 1122 693 169 725 174 391 27 1345
Cuts at: 5035 389
Size: 867
Fragments arranged by size:
1345 1122 867 725 693 391 174 169
27
EagI C'GGCC_G
Cuts at: 3098 3098
Size: 5513
EarI CTCTTCn'nnn_
Cuts at: 1717 2413 3536 3746 1717
Size: 696 1123 210 3484
Fragments arranged by size:
3484 1123 696 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1093 3721 4031 4177 5323 1093
Size: 2628 310 146 1146 1283
Fragments arranged by size:
2628 1283 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1461 2062 2525 1461
Size: 601 463 4449
Fragments arranged by size:
4449 601 463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 418 783 1063 1156 1534 1774 1779 1833
Size: 39 365 280 93 378 240 5 54
Cuts at: 1833 2038 2048 2394 3338 3770 4517 4785 379
Size: 205 10 346 944 432 747 268 1107
Fragments arranged by size:
1107 944 747 432 378 365 346 280
268 240 205 93 54 39 10 5
EcoNI CCTnn'n_nnAGG
Cuts at: 730 1006 2080 2392 730
Size: 276 1074 312 3851
Fragments arranged by size:
3851 1074 312 276
EcoO109I rG'GnC_Cy
Cuts at: 315 316 1038 1052 1740 1741 2168 2219 2652
Size: 1 722 14 688 1 427 51 433
Cuts at: 2652 315
Size: 3176
Fragments arranged by size:
3176 722 688 433 427 51 14 1
1
EcoRI G'AATT_C
Cuts at: 2517 2517
Size: 5513
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 5513
FseI GG_CCGG'CC
Cuts at: 473 473
Size: 5513
FspI TGC'GCA
Cuts at: 340 1699 3294 340
Size: 1359 1595 2559
Fragments arranged by size:
2559 1595 1359
HaeII r_GCGC'y
Cuts at: 273 925 939 2947 3195 4217 5196 273
Size: 652 14 2008 248 1022 979 590
Fragments arranged by size:
2008 1022 979 652 590 248 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 158 190 228 260 3355 3385 3387 3417 158
Size: 32 38 32 3095 30 2 30 2254
Fragments arranged by size:
3095 2254 38 32 32 30 30 2
HincII GTy'rAC
Cuts at: 138 1917 2552 138
Size: 1779 635 3099
Fragments arranged by size:
3099 1779 635
KasI G'GCGC_C
Cuts at: 269 2943 3191 269
Size: 2674 248 2591
Fragments arranged by size:
2674 2591 248
KpnI G_GTAC'C
Cuts at: 1165 2533 1165
Size: 1368 4145
MluI A'CGCG_T
Cuts at: 4946 4946
Size: 5513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 536 2315 2450 4184 4368 536
Size: 1779 135 1734 184 1681
Fragments arranged by size:
1779 1734 1681 184 135
MscI TGG'CCA
Cuts at: 391 2375 3274 391
Size: 1984 899 2630
Fragments arranged by size:
2630 1984 899
MslI CAynn'nnrTG
Cuts at: 304 520 1408 1513 3502 3629 304
Size: 216 888 105 1989 127 2188
Fragments arranged by size:
2188 1989 888 216 127 105
MspA1I CmG'CkG
Cuts at: 329 659 756 863 1626 1692 2414 2624 2938
Size: 330 97 107 763 66 722 210 314
Cuts at: 2938 3298 4311 4556 329
Size: 360 1013 245 1286
Fragments arranged by size:
1286 1013 763 722 360 330 314 245
210 107 97 66
NaeI GCC'GGC
Cuts at: 426 471 583 2421 2443 3694 426
Size: 45 112 1838 22 1251 2245
Fragments arranged by size:
2245 1838 1251 112 45 22
NarI GG'CG_CC
Cuts at: 270 2944 3192 270
Size: 2674 248 2591
Fragments arranged by size:
2674 2591 248
NcoI C'CATG_G
Cuts at: 1110 1599 1110
Size: 489 5024
NgoMIV G'CCGG_C
Cuts at: 424 469 581 2419 2441 3692 424
Size: 45 112 1838 22 1251 2245
Fragments arranged by size:
2245 1838 1251 112 45 22
NspI r_CATG'y
Cuts at: 2566 3066 3973 2566
Size: 500 907 4106
Fragments arranged by size:
4106 907 500
PasI CC'CwG_GG
Cuts at: 463 463
Size: 5513
PciI A'CATG_T
Cuts at: 3969 3969
Size: 5513
PflMI CCAn_nnn'nTGG
Cuts at: 846 939 846
Size: 93 5420
PmeI GTTT'AAAC
Cuts at: 2512 2512
Size: 5513
PmlI CAC'GTG
Cuts at: 1905 1905
Size: 5513
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 4652
Size: 32 5481
PpuMI rG'GwC_Cy
Cuts at: 1038 1038
Size: 5513
PspOMI G'GGCC_C
Cuts at: 315 1051 1740 315
Size: 736 689 4088
Fragments arranged by size:
4088 736 689
PspXI vC'TCGA_Gb
Cuts at: 2134 2134
Size: 5513
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2335 2344 2367 2376 2335
Size: 9 23 9 5472
Fragments arranged by size:
5472 23 9 9
PstI C_TGCA'G
Cuts at: 1148 1241 1787 2059 2312 2393 2560 3245 1148
Size: 93 546 272 253 81 167 685 3416
Fragments arranged by size:
3416 685 546 272 253 167 93 81
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5513
PvuII CAG'CTG
Cuts at: 1692 2414 2938 3298 1692
Size: 722 524 360 3907
Fragments arranged by size:
3907 722 524 360
RsrII CG'GwC_CG
Cuts at: 3708 3708
Size: 5513
SacI G_AGCT'C
Cuts at: 1463 2064 2527 1463
Size: 601 463 4449
Fragments arranged by size:
4449 601 463
SacII CC_GC'GG
Cuts at: 330 330
Size: 5513
SalI G'TCGA_C
Cuts at: 1915 2550 1915
Size: 635 4878
SapI GCTCTTCn'nnn_
Cuts at: 2413 3536 3746 2413
Size: 1123 210 4180
Fragments arranged by size:
4180 1123 210
SbfI CC_TGCA'GG
Cuts at: 2560 2560
Size: 5513
ScaI AGT'ACT
Cuts at: 5030 5030
Size: 5513
SfcI C'TryA_G
Cuts at: 33 1144 1237 1783 2055 2308 2389 2556 3241
Size: 1111 93 546 272 253 81 167 685
Cuts at: 3241 4234 4425 33
Size: 993 191 1121
Fragments arranged by size:
1121 1111 993 685 546 272 253 191
167 93 81
SfiI GGCCn_nnn'nGGCC
Cuts at: 2203 2203
Size: 5513
SfoI GGC'GCC
Cuts at: 271 2945 3193 271
Size: 2674 248 2591
Fragments arranged by size:
2674 2591 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
SmaI CCC'GGG
Cuts at: 2535 2535
Size: 5513
SmlI C'TyrA_G
Cuts at: 2134 2302 2668 3583 4075 4337 2134
Size: 168 366 915 492 262 3310
Fragments arranged by size:
3310 915 492 366 262 168
SnaBI TAC'GTA
Cuts at: 2505 2505
Size: 5513
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5513
SphI G_CATG'C
Cuts at: 2566 3066 2566
Size: 500 5013
StyI C'CwwG_G
Cuts at: 1056 1110 1599 2647 3001 1056
Size: 54 489 1048 354 3568
Fragments arranged by size:
3568 1048 489 354 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 618 807 976 2143 3096 3229 3688 3897 618
Size: 189 169 1167 953 133 459 209 2234
Fragments arranged by size:
2234 1167 953 459 209 189 169 133
TatI w'GTAC_w
Cuts at: 1610 1997 5028 1610
Size: 387 3031 2095
Fragments arranged by size:
3031 2095 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1085 1117 5030 5062 1085
Size: 32 3913 32 1536
Fragments arranged by size:
3913 1536 32 32
Tth111I GACn'n_nGTC
Cuts at: 3310 3310
Size: 5513
XbaI T'CTAG_A
Cuts at: 2544 2544
Size: 5513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3008 3008
Size: 5513
XhoI C'TCGA_G
Cuts at: 2134 2134
Size: 5513
XmaI C'CCGG_G
Cuts at: 2533 2533
Size: 5513
ZraI GAC'GTC
Cuts at: 242 242
Size: 5513
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI BaeI BbsI BbvCI
BcgI BfrBI BmtI BsaXI BsiWI BsmI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PshAI PsiI SanDI SexAI SgrAI SrfI SspI StuI
SwaI XmnI