Restriction map for
pF1KSDA2038
(Circular) (Six-base) MAPSORT of: pF1KSDA2038.seq Check: 5655 from: 1 to: 5761
pF1KSDA2038 5761 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 2797 2797
Size: 5761
Acc65I G'GTAC_C
Cuts at: 2775 2775
Size: 5761
AclI AA'CG_TT
Cuts at: 5601 5601
Size: 5761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 483 819 2036 2381 3584 4016 4765 5033
Size: 66 336 1217 345 1203 432 749 268
Cuts at: 5033 417
Size: 1145
Fragments arranged by size:
1217 1203 1145 749 432 345 336 268
66
AfeI AGC'GCT
Cuts at: 628 1177 1993 628
Size: 549 816 4396
Fragments arranged by size:
4396 816 549
AflIII A'CryG_T
Cuts at: 3111 4217 5194 3111
Size: 1106 977 3678
Fragments arranged by size:
3678 1106 977
AgeI A'CCGG_T
Cuts at: 4149 4149
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1204 1204
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2549 2665 2549
Size: 116 5645
AlwNI CAG_nnn'CTG
Cuts at: 203 1113 2266 3064 4633 203
Size: 910 1153 798 1569 1331
Fragments arranged by size:
1569 1331 1153 910 798
ApaI G_GGCC'C
Cuts at: 350 1054 1439 1884 2075 350
Size: 704 385 445 191 4036
Fragments arranged by size:
4036 704 445 385 191
ApaLI G'TGCA_C
Cuts at: 4531 4531
Size: 5761
ApoI r'AATT_y
Cuts at: 2763 5114 5370 2763
Size: 2351 256 3154
Fragments arranged by size:
3154 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5761
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5761
AvaI C'yCGr_G
Cuts at: 404 1288 1378 1438 1598 1877 2085 2419 2480
Size: 884 90 60 160 279 208 334 61
Cuts at: 2480 2621 2779 404
Size: 141 158 3386
Fragments arranged by size:
3386 884 334 279 208 160 158 141
90 61 60
BamHI G'GATC_C
Cuts at: 2784 2784
Size: 5761
BanI G'GyrC_C
Cuts at: 172 739 930 1056 1215 2453 2775 3189 3437
Size: 567 191 126 159 1238 322 414 248
Cuts at: 3437 3472 172
Size: 35 2461
Fragments arranged by size:
2461 1238 567 414 322 248 191 159
126 35
BanII G_rGCy'C
Cuts at: 350 578 1054 1229 1295 1360 1385 1439 1469
Size: 228 476 175 66 65 25 54 30
Cuts at: 1469 1884 2013 2075 2225 2291 2594 2773 3803
Size: 415 129 62 150 66 303 179 1030
Cuts at: 3803 350
Size: 2308
Fragments arranged by size:
2308 1030 476 415 303 228 179 175
150 129 66 66 65 62 54 30
25
BbeI G_GCGC'C
Cuts at: 176 743 1060 1219 2457 3193 3441 176
Size: 567 317 159 1238 736 248 2496
Fragments arranged by size:
2496 1238 736 567 317 248 159
BbsI GAAGACnn'nnnn_
Cuts at: 453 1241 2057 453
Size: 788 816 4157
Fragments arranged by size:
4157 816 788
BciVI GTATCCnnnnn_n'
Cuts at: 3657 4420 5761 3657
Size: 763 1341 3657
Fragments arranged by size:
3657 1341 763
BclI T'GATC_A
Cuts at: 3279 3279
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 148 939 1049 1464 1879 148
Size: 791 110 415 415 4030
Fragments arranged by size:
4030 791 415 415 110
BglII A'GATC_T
Cuts at: 2744 3274 5313 2744
Size: 530 2039 3192
Fragments arranged by size:
3192 2039 530
BlpI GC'TnA_GC
Cuts at: 2871 2871
Size: 5761
Bme1580I G_kGCm'C
Cuts at: 350 935 1054 1439 1884 2075 3384 3477 4535
Size: 585 119 385 445 191 1309 93 1058
Cuts at: 4535 350
Size: 1576
Fragments arranged by size:
1576 1309 1058 585 445 385 191 119
93
BmrI ACTGGGnnnn_n'
Cuts at: 1374 2971 3052 3387 5533 1374
Size: 1597 81 335 2146 1602
Fragments arranged by size:
2146 1602 1597 335 81
BmtI G_CTAG'C
Cuts at: 1318 1318
Size: 5761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2583 4126 2583
Size: 1543 4218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 343 874 1514 1571 1611 1671 1699 2239
Size: 160 531 640 57 40 60 28 540
Cuts at: 2239 2243 2578 2754 183
Size: 4 335 176 3190
Fragments arranged by size:
3190 640 540 531 335 176 160 60
57 40 28 4
Bpu10I CC'TnA_GC
Cuts at: 805 2267 805
Size: 1462 4299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2935 3814 4308 4606 2935
Size: 879 494 298 4090
Fragments arranged by size:
4090 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 2571 5465 2571
Size: 2894 2867
BsaAI yAC'GTr
Cuts at: 1353 2751 3112 1353
Size: 1398 361 4002
Fragments arranged by size:
4002 1398 361
BsaBI GATnn'nnATC
Cuts at: 6 358 3273 5312 6
Size: 352 2915 2039 455
Fragments arranged by size:
2915 2039 455 352
BsaHI Gr'CG_yC
Cuts at: 173 254 740 1057 1216 2454 3190 3438 4142
Size: 81 486 317 159 1238 736 248 704
Cuts at: 4142 5638 173
Size: 1496 296
Fragments arranged by size:
1496 1238 736 704 486 317 296 248
159 81
BsaWI w'CCGG_w
Cuts at: 2948 3469 4149 4423 4570 4981 2948
Size: 521 680 274 147 411 3728
Fragments arranged by size:
3728 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 589 619 1144 1174 1180 1210 1462 1492 1753
Size: 30 525 30 6 30 252 30 261
Cuts at: 1753 1783 589
Size: 30 4567
Fragments arranged by size:
4567 525 261 252 30 30 30 30
30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 460 1429 1576 1695 1952 2157 2172 2175
Size: 120 969 147 119 257 205 15 3
Cuts at: 2175 2178 2444 2508 2575 340
Size: 3 266 64 67 3526
Fragments arranged by size:
3526 969 266 257 205 147 120 119
67 64 15 3 3
BseYI C'CCAG_C
Cuts at: 657 699 934 1026 1681 1830 1864 2127 2241
Size: 42 235 92 655 149 34 263 114
Cuts at: 2241 3184 3942 4521 5017 657
Size: 943 758 579 496 1401
Fragments arranged by size:
1401 943 758 655 579 496 263 235
149 114 92 42 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1048 2359 2531 2671 949
Size: 99 1311 172 140 4039
Fragments arranged by size:
4039 1311 172 140 99
BsiEI CG_ry'CG
Cuts at: 104 143 943 1205 1456 1620 1905 3301 3347
Size: 39 800 262 251 164 285 1396 46
Cuts at: 3347 4557 104
Size: 1210 1308
Fragments arranged by size:
1396 1308 1210 800 285 262 251 164
46 39
BsiHKAI G_wGCw'C
Cuts at: 578 881 1229 2013 2436 2773 3551 3741 4535
Size: 303 348 784 423 337 778 190 794
Cuts at: 4535 578
Size: 1804
Fragments arranged by size:
1804 794 784 778 423 348 337 303
190
Bsp1286I G_dGCh'C
Cuts at: 350 578 881 935 1054 1229 1295 1360 1385
Size: 228 303 54 119 175 66 65 25
Cuts at: 1385 1439 1469 1884 2013 2075 2225 2291 2436
Size: 54 30 415 129 62 150 66 145
Cuts at: 2436 2594 2773 3384 3477 3551 3741 3803 4535
Size: 158 179 611 93 74 190 62 732
Cuts at: 4535 350
Size: 1576
Fragments arranged by size:
1576 732 611 415 303 228 190 179
175 158 150 145 129 119 93 74
66 66 65 62 62 54 54 30
25
BspEI T'CCGG_A
Cuts at: 4981 4981
Size: 5761
BspHI T'CATG_A
Cuts at: 4937 4937
Size: 5761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 971 2691 2809 3023 3325 3706 971
Size: 1720 118 214 302 381 3026
Fragments arranged by size:
3026 1720 381 302 214 118
BsrBI CCG'CTC
Cuts at: 50 183 1901 4051 4105 5591 5756 50
Size: 133 1718 2150 54 1486 165 55
Fragments arranged by size:
2150 1718 1486 165 133 55 54
BsrDI GCAATG_nn'
Cuts at: 984 1614 3671 984
Size: 630 2057 3074
Fragments arranged by size:
3074 2057 630
BsrFI r'CCGG_y
Cuts at: 151 290 747 915 938 1320 1330 1619 1668
Size: 139 457 168 23 382 10 289 49
Cuts at: 1668 3757 3938 4149 151
Size: 2089 181 211 1763
Fragments arranged by size:
2089 1763 457 382 289 211 181 168
139 49 23 10
BsrGI T'GTAC_A
Cuts at: 545 545
Size: 5761
BssHII G'CGCG_C
Cuts at: 888 1256 1258 1281 888
Size: 368 2 23 5368
Fragments arranged by size:
5368 368 23 2
BssSI C'ACGA_G
Cuts at: 4030 4390 4030
Size: 360 5401
BstAPI GCAn_nnn'nTGC
Cuts at: 885 972 1071 1113 3136 885
Size: 87 99 42 2023 3510
Fragments arranged by size:
3510 2023 99 87 42
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5761
BstXI CCAn_nnnn'nTGG
Cuts at: 1020 1793 2298 1020
Size: 773 505 4483
Fragments arranged by size:
4483 773 505
BstYI r'GATC_y
Cuts at: 1 599 1403 2744 2784 3251 3274 3609 3855
Size: 598 804 1341 40 467 23 335 246
Cuts at: 3855 4869 4955 4967 5313 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1341 1014 804 598 467 449 346 335
246 86 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 2043 2043
Size: 5761
BtgI C'CryG_G
Cuts at: 125 716 1199 1490 125
Size: 591 483 291 4396
Fragments arranged by size:
4396 591 483 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1739 3709 3890 4053 1739
Size: 1970 181 163 3447
Fragments arranged by size:
3447 1970 181 163
BtsI GCAGTG_nn'
Cuts at: 301 1743 1749 2303 3002 301
Size: 1442 6 554 699 3060
Fragments arranged by size:
3060 1442 699 554 6
ClaI AT'CG_AT
Cuts at: 2633 2633
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2481 2516 2481
Size: 35 5726
DraI TTT'AAA
Cuts at: 2758 2758
Size: 5761
DraIII CAC_nnn'GTG
Cuts at: 885 972 885
Size: 87 5674
DrdI GACnn_nn'nnGTC
Cuts at: 193 1132 1502 3465 4325 193
Size: 939 370 1963 860 1629
Fragments arranged by size:
1963 1629 939 860 370
EaeI y'GGCC_r
Cuts at: 140 149 452 677 940 1118 1202 1453 1617
Size: 9 303 225 263 178 84 251 164
Cuts at: 1617 3344 3518 3909 3936 5283 140
Size: 1727 174 391 27 1347 618
Fragments arranged by size:
1727 1347 618 391 303 263 251 225
178 174 164 84 27 9
EagI C'GGCC_G
Cuts at: 140 940 1202 1453 1617 3344 140
Size: 800 262 251 164 1727 2557
Fragments arranged by size:
2557 1727 800 262 251 164
EarI CTCTTCn'nnn_
Cuts at: 2196 3782 3992 2196
Size: 1586 210 3965
Fragments arranged by size:
3965 1586 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 610 3967 4279 4425 5571 610
Size: 3357 312 146 1146 800
Fragments arranged by size:
3357 1146 800 312 146
EcoICRI GAG'CTC
Cuts at: 576 1227 2011 2771 576
Size: 651 784 760 3566
Fragments arranged by size:
3566 784 760 651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 343 417 483 819 874 1514 1571 1611
Size: 160 74 66 336 55 640 57 40
Cuts at: 1611 1671 1699 2036 2239 2243 2381 2578 2754
Size: 60 28 337 203 4 138 197 176
Cuts at: 2754 3584 4016 4765 5033 183
Size: 830 432 749 268 911
Fragments arranged by size:
911 830 749 640 432 337 336 268
203 197 176 160 138 74 66 60
57 55 40 28 4
EcoNI CCTnn'n_nnAGG
Cuts at: 325 397 2560 325
Size: 72 2163 3526
Fragments arranged by size:
3526 2163 72
EcoO109I rG'GnC_Cy
Cuts at: 1050 1110 1125 1435 1443 1881 2047 2089 2382
Size: 60 15 310 8 438 166 42 293
Cuts at: 2382 2416 2464 2898 1050
Size: 34 48 434 3913
Fragments arranged by size:
3913 438 434 310 293 166 60 48
42 34 15 8
EcoRI G'AATT_C
Cuts at: 2763 2763
Size: 5761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3778 3778
Size: 5761
FseI GG_CCGG'CC
Cuts at: 155 155
Size: 5761
FspI TGC'GCA
Cuts at: 3540 3540
Size: 5761
HaeII r_GCGC'y
Cuts at: 176 630 676 743 1039 1060 1143 1160 1179
Size: 454 46 67 296 21 83 17 19
Cuts at: 1179 1219 1321 1522 1995 2457 3193 3441 4465
Size: 40 102 201 473 462 736 248 1024
Cuts at: 4465 5444 176
Size: 979 493
Fragments arranged by size:
1024 979 736 493 473 462 454 296
248 201 102 83 67 46 40 21
19 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1144 1176 1180 1212 1462 1494 1753 1785 2503
Size: 32 4 32 250 32 259 32 718
Cuts at: 2503 2535 2615 2647 3601 3631 3633 3663 1144
Size: 32 80 32 954 30 2 30 3242
Fragments arranged by size:
3242 954 718 259 250 80 32 32
32 32 32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 2798 2798
Size: 5761
KasI G'GCGC_C
Cuts at: 172 739 1056 1215 2453 3189 3437 172
Size: 567 317 159 1238 736 248 2496
Fragments arranged by size:
2496 1238 736 567 317 248 159
KpnI G_GTAC'C
Cuts at: 2779 2779
Size: 5761
MluI A'CGCG_T
Cuts at: 5194 5194
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4432 4616 4432
Size: 184 5577
MscI TGG'CCA
Cuts at: 454 3520 454
Size: 3066 2695
MslI CAynn'nnrTG
Cuts at: 478 583 778 1204 2647 3748 3875 478
Size: 105 195 426 1443 1101 127 2364
Fragments arranged by size:
2364 1443 1101 426 195 127 105
MspA1I CmG'CkG
Cuts at: 203 718 852 1062 1222 1492 1616 1955 2056
Size: 515 134 210 160 270 124 339 101
Cuts at: 2056 2155 2404 2870 3184 3544 4559 4804 203
Size: 99 249 466 314 360 1015 245 1160
Fragments arranged by size:
1160 1015 515 466 360 339 314 270
249 245 210 160 134 124 101 99
NaeI GCC'GGC
Cuts at: 153 292 940 1322 1332 1621 3940 153
Size: 139 648 382 10 289 2319 1974
Fragments arranged by size:
2319 1974 648 382 289 139 10
NarI GG'CG_CC
Cuts at: 173 740 1057 1216 2454 3190 3438 173
Size: 567 317 159 1238 736 248 2496
Fragments arranged by size:
2496 1238 736 567 317 248 159
NcoI C'CATG_G
Cuts at: 125 125
Size: 5761
NgoMIV G'CCGG_C
Cuts at: 151 290 938 1320 1330 1619 3938 151
Size: 139 648 382 10 289 2319 1974
Fragments arranged by size:
2319 1974 648 382 289 139 10
NheI G'CTAG_C
Cuts at: 1314 1314
Size: 5761
NruI TCG'CGA
Cuts at: 4122 4122
Size: 5761
NspI r_CATG'y
Cuts at: 565 2812 3312 4221 565
Size: 2247 500 909 2105
Fragments arranged by size:
2247 2105 909 500
PasI CC'CwG_GG
Cuts at: 376 1462 376
Size: 1086 4675
PciI A'CATG_T
Cuts at: 4217 4217
Size: 5761
PfoI T'CCnGG_A
Cuts at: 392 1715 2216 392
Size: 1323 501 3937
Fragments arranged by size:
3937 1323 501
PmeI GTTT'AAAC
Cuts at: 2758 2758
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4900 4932 4900
Size: 32 5729
PpuMI rG'GwC_Cy
Cuts at: 1125 2089 2382 2416 1125
Size: 964 293 34 4470
Fragments arranged by size:
4470 964 293 34
PspOMI G'GGCC_C
Cuts at: 346 1050 1435 1880 2071 346
Size: 704 385 445 191 4036
Fragments arranged by size:
4036 704 445 385 191
PstI C_TGCA'G
Cuts at: 371 863 1667 2414 2702 2806 3491 371
Size: 492 804 747 288 104 685 2641
Fragments arranged by size:
2641 804 747 685 492 288 104
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5761
PvuII CAG'CTG
Cuts at: 1955 2155 2404 3184 3544 1955
Size: 200 249 780 360 4172
Fragments arranged by size:
4172 780 360 249 200
RsrII CG'GwC_CG
Cuts at: 3954 3954
Size: 5761
SacI G_AGCT'C
Cuts at: 578 1229 2013 2773 578
Size: 651 784 760 3566
Fragments arranged by size:
3566 784 760 651
SacII CC_GC'GG
Cuts at: 719 1493 719
Size: 774 4987
SalI G'TCGA_C
Cuts at: 2796 2796
Size: 5761
SanDI GG'GwC_CC
Cuts at: 1125 2416 1125
Size: 1291 4470
SapI GCTCTTCn'nnn_
Cuts at: 3782 3992 3782
Size: 210 5551
SbfI CC_TGCA'GG
Cuts at: 2806 2806
Size: 5761
ScaI AGT'ACT
Cuts at: 1195 5278 1195
Size: 4083 1678
SexAI A'CCwGG_T
Cuts at: 2378 2378
Size: 5761
SfcI C'TryA_G
Cuts at: 33 367 859 1663 2410 2698 2802 3487 4482
Size: 334 492 804 747 288 104 685 995
Cuts at: 4482 4673 33
Size: 191 1121
Fragments arranged by size:
1121 995 804 747 685 492 334 288
191 104
SfiI GGCCn_nnn'nGGCC
Cuts at: 148 1879 148
Size: 1731 4030
SfoI GGC'GCC
Cuts at: 174 741 1058 1217 2455 3191 3439 174
Size: 567 317 159 1238 736 248 2496
Fragments arranged by size:
2496 1238 736 567 317 248 159
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1330 1330
Size: 5761
SmaI CCC'GGG
Cuts at: 406 1290 1600 1879 2482 2781 406
Size: 884 310 279 603 299 3386
Fragments arranged by size:
3386 884 603 310 299 279
SmlI C'TyrA_G
Cuts at: 2914 3829 4323 4585 2914
Size: 915 494 262 4090
Fragments arranged by size:
4090 915 494 262
SnaBI TAC'GTA
Cuts at: 2751 2751
Size: 5761
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5761
SphI G_CATG'C
Cuts at: 565 2812 3312 565
Size: 2247 500 3014
Fragments arranged by size:
3014 2247 500
StuI AGG'CCT
Cuts at: 1873 2146 2350 1873
Size: 273 204 5284
Fragments arranged by size:
5284 273 204
StyI C'CwwG_G
Cuts at: 125 228 620 2345 2448 2893 3247 125
Size: 103 392 1725 103 445 354 2639
Fragments arranged by size:
2639 1725 445 392 354 103 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3342 3475 3934 4145 3342
Size: 133 459 211 4958
Fragments arranged by size:
4958 459 211 133
TatI w'GTAC_w
Cuts at: 545 1193 5276 545
Size: 648 4083 1030
Fragments arranged by size:
4083 1030 648
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 589 621 2073 2105 5278 5310 589
Size: 32 1452 32 3173 32 1040
Fragments arranged by size:
3173 1452 1040 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3556 3556
Size: 5761
XbaI T'CTAG_A
Cuts at: 2790 2790
Size: 5761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 3254 132
Size: 3122 2639
XmaI C'CCGG_G
Cuts at: 404 1288 1598 1877 2480 2779 404
Size: 884 310 279 603 299 3386
Fragments arranged by size:
3386 884 603 310 299 279
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI
PasI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BsiWI BsmI BsmBI BstEII
BstZ17I EcoRV FspAI HindIII HpaI MfeI NdeI NotI
NsiI PacI PflMI PmlI PshAI PsiI PspXI PsrI
SrfI SspI SwaI XhoI XmnI ZraI