Restriction map for
pF1KSDB0002
(Circular) (Six-base) MAPSORT of: pF1KSDB0002.seq Check: 5897 from: 1 to: 6865
pF1KSDB0002 6865 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 831 831
Size: 6865
AccI GT'mk_AC
Cuts at: 3901 3901
Size: 6865
Acc65I G'GTAC_C
Cuts at: 443 3879 443
Size: 3436 3429
AclI AA'CG_TT
Cuts at: 6705 6705
Size: 6865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 4688 5120 5869 6137 649
Size: 4039 432 749 268 1377
Fragments arranged by size:
4039 1377 749 432 268
AflIII A'CryG_T
Cuts at: 2327 4215 5321 6298 2327
Size: 1888 1106 977 2894
Fragments arranged by size:
2894 1888 1106 977
AgeI A'CCGG_T
Cuts at: 5253 5253
Size: 6865
AleI CACnn'nnGTG
Cuts at: 470 3346 3405 3460 470
Size: 2876 59 55 3875
Fragments arranged by size:
3875 2876 59 55
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 488 3490 488
Size: 3002 3863
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1669 1701 1669
Size: 32 6833
AlwNI CAG_nnn'CTG
Cuts at: 764 1430 2634 3247 4168 5737 764
Size: 666 1204 613 921 1569 1892
Fragments arranged by size:
1892 1569 1204 921 666 613
ApaI G_GGCC'C
Cuts at: 247 824 1544 2748 3025 3313 247
Size: 577 720 1204 277 288 3799
Fragments arranged by size:
3799 1204 720 577 288 277
ApaLI G'TGCA_C
Cuts at: 3708 5635 3708
Size: 1927 4938
ApoI r'AATT_y
Cuts at: 2824 3867 6218 6474 2824
Size: 1043 2351 256 3215
Fragments arranged by size:
3215 2351 1043 256
AscI GG'CGCG_CC
Cuts at: 250 250
Size: 6865
AseI AT'TA_AT
Cuts at: 20 20
Size: 6865
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6865
AvaI C'yCGr_G
Cuts at: 256 389 505 823 1583 2455 2741 2747 2802
Size: 133 116 318 760 872 286 6 55
Cuts at: 2802 3034 3062 3344 3458 3883 256
Size: 232 28 282 114 425 3238
Fragments arranged by size:
3238 872 760 425 318 286 282 232
133 116 114 55 28 6
BamHI G'GATC_C
Cuts at: 2718 3888 2718
Size: 1170 5695
BanI G'GyrC_C
Cuts at: 443 983 1126 1916 2473 3407 3879 4293 4541
Size: 540 143 790 557 934 472 414 248
Cuts at: 4541 4576 443
Size: 35 2732
Fragments arranged by size:
2732 934 790 557 540 472 414 248
143 35
BanII G_rGCy'C
Cuts at: 136 247 824 1544 2035 2606 2748 2795 2803
Size: 111 577 720 491 571 142 47 8
Cuts at: 2803 3025 3313 3603 3877 4907 136
Size: 222 288 290 274 1030 2094
Fragments arranged by size:
2094 1030 720 577 571 491 290 288
274 222 142 111 47 8
BbeI G_GCGC'C
Cuts at: 4297 4545 4297
Size: 248 6617
BbsI GAAGACnn'nnnn_
Cuts at: 1744 1744
Size: 6865
BbvCI CC'TCA_GC
Cuts at: 3237 3577 3237
Size: 340 6525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 385 419 478 512 2985 3019 385
Size: 34 59 34 2473 34 4231
Fragments arranged by size:
4231 2473 59 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2575 4761 5524 6865 2575
Size: 2186 763 1341 2575
Fragments arranged by size:
2575 2186 1341 763
BclI T'GATC_A
Cuts at: 2835 4383 2835
Size: 1548 5317
BglI GCCn_nnn'nGGC
Cuts at: 235 412 508 898 2006 2703 3217 235
Size: 177 96 390 1108 697 514 3883
Fragments arranged by size:
3883 1108 697 514 390 177 96
BglII A'GATC_T
Cuts at: 1671 4378 6417 1671
Size: 2707 2039 2119
Fragments arranged by size:
2707 2119 2039
BlpI GC'TnA_GC
Cuts at: 1510 3975 1510
Size: 2465 4400
Bme1580I G_kGCm'C
Cuts at: 247 590 824 1544 1630 1859 1919 1994 2478
Size: 343 234 720 86 229 60 75 484
Cuts at: 2478 2748 3025 3074 3313 3479 3700 3712 4488
Size: 270 277 49 239 166 221 12 776
Cuts at: 4488 4581 5639 247
Size: 93 1058 1473
Fragments arranged by size:
1473 1058 776 720 484 343 277 270
239 234 229 221 166 93 86 75
60 49 12
BmgBI CAC'GTC
Cuts at: 162 585 162
Size: 423 6442
BmrI ACTGGGnnnn_n'
Cuts at: 1743 1981 1988 2269 3008 3028 3269 3443 4075
Size: 238 7 281 739 20 241 174 632
Cuts at: 4075 4156 4491 6637 1743
Size: 81 335 2146 1971
Fragments arranged by size:
2146 1971 739 632 335 281 241 238
174 81 20 7
BmtI G_CTAG'C
Cuts at: 3047 3047
Size: 6865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1437 3453 3792 5230 1437
Size: 2016 339 1438 3072
Fragments arranged by size:
3072 2016 1438 339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1095 1407 2620 2771 2890 907
Size: 188 312 1213 151 119 4882
Fragments arranged by size:
4882 1213 312 188 151 119
Bpu10I CC'TnA_GC
Cuts at: 476 3237 3577 476
Size: 2761 340 3764
Fragments arranged by size:
3764 2761 340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 591 3251 3774 4039 4918 5412 5710 343
Size: 248 2660 523 265 879 494 298 1498
Fragments arranged by size:
2660 1498 879 523 494 298 265 248
BsaI GGTCTCn'nnnn_
Cuts at: 871 6569 871
Size: 5698 1167
BsaAI yAC'GTr
Cuts at: 3129 3855 4216 3129
Size: 726 361 5778
Fragments arranged by size:
5778 726 361
BsaBI GATnn'nnATC
Cuts at: 6 667 754 4377 6416 6
Size: 661 87 3623 2039 455
Fragments arranged by size:
3623 2039 661 455 87
BsaHI Gr'CG_yC
Cuts at: 2079 4294 4542 5246 6742 2079
Size: 2215 248 704 1496 2202
Fragments arranged by size:
2215 2202 1496 704 248
BsaWI w'CCGG_w
Cuts at: 138 1439 1464 4052 4573 5253 5527 5674 6085
Size: 1301 25 2588 521 680 274 147 411
Cuts at: 6085 138
Size: 918
Fragments arranged by size:
2588 1301 918 680 521 411 274 147
25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1228 1258 1828 1858 1228
Size: 30 570 30 6235
Fragments arranged by size:
6235 570 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1395 1516 1519 1786 1891 2482 2956 3205 3219
Size: 121 3 267 105 591 474 249 14
Cuts at: 3219 3429 1395
Size: 210 4831
Fragments arranged by size:
4831 591 474 267 249 210 121 105
14 3
BseYI C'CCAG_C
Cuts at: 238 559 1006 1140 1171 1384 1645 2125 2161
Size: 321 447 134 31 213 261 480 36
Cuts at: 2161 2393 2447 2605 2900 3073 3303 3634 4288
Size: 232 54 158 295 173 230 331 654
Cuts at: 4288 5046 5625 6121 238
Size: 758 579 496 982
Fragments arranged by size:
982 758 654 579 496 480 447 331
321 295 261 232 230 213 173 158
134 54 36 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1204 1590 2764 3011 3413 448
Size: 756 386 1174 247 402 3900
Fragments arranged by size:
3900 1174 756 402 386 247
BsiEI CG_ry'CG
Cuts at: 104 734 4405 4451 5661 104
Size: 630 3671 46 1210 1308
Fragments arranged by size:
3671 1308 1210 630 46
BsiHKAI G_wGCw'C
Cuts at: 401 2998 3712 3877 4655 4845 5639 401
Size: 2597 714 165 778 190 794 1627
Fragments arranged by size:
2597 1627 794 778 714 190 165
BsmI GAATG_Cn'
Cuts at: 601 601
Size: 6865
Bsp1286I G_dGCh'C
Cuts at: 136 247 401 590 824 1544 1630 1859 1919
Size: 111 154 189 234 720 86 229 60
Cuts at: 1919 1994 2035 2478 2606 2748 2795 2803 2998
Size: 75 41 443 128 142 47 8 195
Cuts at: 2998 3025 3074 3313 3479 3603 3700 3712 3877
Size: 27 49 239 166 124 97 12 165
Cuts at: 3877 4488 4581 4655 4845 4907 5639 136
Size: 611 93 74 190 62 732 1362
Fragments arranged by size:
1362 732 720 611 443 239 234 229
195 190 189 166 165 154 142 128
124 111 97 93 86 75 74 62
60 49 47 41 27 12 8
BspEI T'CCGG_A
Cuts at: 138 1439 1464 6085 138
Size: 1301 25 4621 918
Fragments arranged by size:
4621 1301 918 25
BspHI T'CATG_A
Cuts at: 6041 6041
Size: 6865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 188 831 3913 4127 4429 4810 188
Size: 643 3082 214 302 381 2243
Fragments arranged by size:
3082 2243 643 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1230 1244 1865 3487 3788 5155 5209 6695
Size: 1180 14 621 1622 301 1367 54 1486
Cuts at: 6695 6860 50
Size: 165 55
Fragments arranged by size:
1622 1486 1367 1180 621 301 165 55
54 14
BsrDI GCAATG_nn'
Cuts at: 1599 2422 3179 4775 1599
Size: 823 757 1596 3689
Fragments arranged by size:
3689 1596 823 757
BsrFI r'CCGG_y
Cuts at: 980 1196 1257 3410 4861 5042 5253 980
Size: 216 61 2153 1451 181 211 2592
Fragments arranged by size:
2592 2153 1451 216 211 181 61
BssHII G'CGCG_C
Cuts at: 222 250 222
Size: 28 6837
BssSI C'ACGA_G
Cuts at: 874 1629 2700 5134 5494 874
Size: 755 1071 2434 360 2245
Fragments arranged by size:
2434 2245 1071 755 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1663 2775 3247 4240 1663
Size: 1112 472 993 4288
Fragments arranged by size:
4288 1112 993 472
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6865
BstXI CCAn_nnnn'nTGG
Cuts at: 642 1014 1074 3462 642
Size: 372 60 2388 4045
Fragments arranged by size:
4045 2388 372 60
BstYI r'GATC_y
Cuts at: 1 1671 2431 2718 3888 4355 4378 4713 4959
Size: 1670 760 287 1170 467 23 335 246
Cuts at: 4959 5973 6059 6071 6417 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1670 1170 1014 760 467 449 346 335
287 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2384 3701 2384
Size: 1317 5548
BtgI C'CryG_G
Cuts at: 125 734 1323 1351 1494 3215 125
Size: 609 589 28 143 1721 3775
Fragments arranged by size:
3775 1721 609 589 143 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 161 393 1403 4813 4994 5157 161
Size: 232 1010 3410 181 163 1869
Fragments arranged by size:
3410 1869 1010 232 181 163
BtsI GCAGTG_nn'
Cuts at: 1461 1821 2016 3074 4106 1461
Size: 360 195 1058 1032 4220
Fragments arranged by size:
4220 1058 1032 360 195
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3606 3641 3606
Size: 35 6830
DraI TTT'AAA
Cuts at: 3862 3862
Size: 6865
DraIII CAC_nnn'GTG
Cuts at: 375 3451 375
Size: 3076 3789
DrdI GACnn_nn'nnGTC
Cuts at: 4569 5429 4569
Size: 860 6005
EaeI y'GGCC_r
Cuts at: 227 731 768 1064 1280 1320 1596 1998 4448
Size: 504 37 296 216 40 276 402 2450
Cuts at: 4448 4622 5013 5040 6387 227
Size: 174 391 27 1347 705
Fragments arranged by size:
2450 1347 705 504 402 391 296 276
216 174 40 37 27
EagI C'GGCC_G
Cuts at: 731 4448 731
Size: 3717 3148
EarI CTCTTCn'nnn_
Cuts at: 1351 2556 2921 4886 5096 1351
Size: 1205 365 1965 210 3120
Fragments arranged by size:
3120 1965 1205 365 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 430 907 1836 2482 3429 5071 5383 5529 6675
Size: 477 929 646 947 1642 312 146 1146
Cuts at: 6675 430
Size: 620
Fragments arranged by size:
1642 1146 947 929 646 620 477 312
146
EcoICRI GAG'CTC
Cuts at: 3875 3875
Size: 6865
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 907 1095 1407 2620 2771 2890 4688 5120
Size: 258 188 312 1213 151 119 1798 432
Cuts at: 5120 5869 6137 649
Size: 749 268 1377
Fragments arranged by size:
1798 1377 1213 749 432 312 268 258
188 151 119
EcoNI CCTnn'n_nnAGG
Cuts at: 553 2309 553
Size: 1756 5109
EcoO109I rG'GnC_Cy
Cuts at: 243 595 639 820 1540 1541 2388 3022 3309
Size: 352 44 181 720 1 847 634 287
Cuts at: 3309 3319 4002 243
Size: 10 683 3106
Fragments arranged by size:
3106 847 720 683 634 352 287 181
44 10 1
EcoRI G'AATT_C
Cuts at: 3867 3867
Size: 6865
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4882 4882
Size: 6865
FspI TGC'GCA
Cuts at: 166 184 4644 166
Size: 18 4460 2387
Fragments arranged by size:
4460 2387 18
HaeII r_GCGC'y
Cuts at: 302 765 1214 2454 3338 3809 4297 4545 5569
Size: 463 449 1240 884 471 488 248 1024
Cuts at: 5569 6548 302
Size: 979 619
Fragments arranged by size:
1240 1024 979 884 619 488 471 463
449 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 171 2052 2084 2533 2565 3096 3128 3249
Size: 32 1881 32 449 32 531 32 121
Cuts at: 3249 3281 4705 4735 4737 4767 139
Size: 32 1424 30 2 30 2237
Fragments arranged by size:
2237 1881 1424 531 449 121 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3902 3902
Size: 6865
KasI G'GCGC_C
Cuts at: 4293 4541 4293
Size: 248 6617
KpnI G_GTAC'C
Cuts at: 447 3883 447
Size: 3436 3429
MluI A'CGCG_T
Cuts at: 6298 6298
Size: 6865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 971 2372 2390 5536 5720 971
Size: 1401 18 3146 184 2116
Fragments arranged by size:
3146 2116 1401 184 18
MscI TGG'CCA
Cuts at: 770 1322 1598 4624 770
Size: 552 276 3026 3011
Fragments arranged by size:
3026 3011 552 276
MslI CAynn'nnrTG
Cuts at: 470 796 1252 1783 1949 1966 2116 2249 2326
Size: 326 456 531 166 17 150 133 77
Cuts at: 2326 2953 3097 3103 3346 3405 3460 4852 4979
Size: 627 144 6 243 59 55 1392 127
Cuts at: 4979 470
Size: 2356
Fragments arranged by size:
2356 1392 627 531 456 326 243 166
150 144 133 127 77 59 55 17
6
MspA1I CmG'CkG
Cuts at: 440 462 530 536 730 747 1268 1496 1604
Size: 22 68 6 194 17 521 228 108
Cuts at: 1604 2129 2288 2668 3145 3764 3974 4288 4648
Size: 525 159 380 477 619 210 314 360
Cuts at: 4648 5663 5908 440
Size: 1015 245 1397
Fragments arranged by size:
1397 1015 619 525 521 477 380 360
314 245 228 210 194 159 108 68
22 17 6
NaeI GCC'GGC
Cuts at: 982 1259 3412 5044 982
Size: 277 2153 1632 2803
Fragments arranged by size:
2803 2153 1632 277
NarI GG'CG_CC
Cuts at: 4294 4542 4294
Size: 248 6617
NcoI C'CATG_G
Cuts at: 125 1323 3215 125
Size: 1198 1892 3775
Fragments arranged by size:
3775 1892 1198
NgoMIV G'CCGG_C
Cuts at: 980 1257 3410 5042 980
Size: 277 2153 1632 2803
Fragments arranged by size:
2803 2153 1632 277
NheI G'CTAG_C
Cuts at: 3043 3043
Size: 6865
NruI TCG'CGA
Cuts at: 5226 5226
Size: 6865
NspI r_CATG'y
Cuts at: 201 873 1965 2331 3171 3916 4416 5325 201
Size: 672 1092 366 840 745 500 909 1741
Fragments arranged by size:
1741 1092 909 840 745 672 500 366
PasI CC'CwG_GG
Cuts at: 1473 1975 2712 3206 3756 1473
Size: 502 737 494 550 4582
Fragments arranged by size:
4582 737 550 502 494
PciI A'CATG_T
Cuts at: 2327 5321 2327
Size: 2994 3871
PflMI CCAn_nnn'nTGG
Cuts at: 1810 2094 1810
Size: 284 6581
PfoI T'CCnGG_A
Cuts at: 884 2197 884
Size: 1313 5552
PmeI GTTT'AAAC
Cuts at: 3862 3862
Size: 6865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6004 6036 6004
Size: 32 6833
PpuMI rG'GwC_Cy
Cuts at: 595 2388 3319 595
Size: 1793 931 4141
Fragments arranged by size:
4141 1793 931
PspOMI G'GGCC_C
Cuts at: 243 820 1540 2744 3021 3309 243
Size: 577 720 1204 277 288 3799
Fragments arranged by size:
3799 1204 720 577 288 277
PspXI vC'TCGA_Gb
Cuts at: 256 256
Size: 6865
PstI C_TGCA'G
Cuts at: 535 746 1671 2639 3189 3910 4595 535
Size: 211 925 968 550 721 685 2805
Fragments arranged by size:
2805 968 925 721 685 550 211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6865
PvuII CAG'CTG
Cuts at: 440 462 530 747 2668 3145 4288 4648 440
Size: 22 68 217 1921 477 1143 360 2657
Fragments arranged by size:
2657 1921 1143 477 360 217 68 22
RsrII CG'GwC_CG
Cuts at: 5058 5058
Size: 6865
SacI G_AGCT'C
Cuts at: 3877 3877
Size: 6865
SacII CC_GC'GG
Cuts at: 1497 1497
Size: 6865
SalI G'TCGA_C
Cuts at: 3900 3900
Size: 6865
SapI GCTCTTCn'nnn_
Cuts at: 4886 5096 4886
Size: 210 6655
SbfI CC_TGCA'GG
Cuts at: 3910 3910
Size: 6865
ScaI AGT'ACT
Cuts at: 262 6382 262
Size: 6120 745
SexAI A'CCwGG_T
Cuts at: 371 371
Size: 6865
SfcI C'TryA_G
Cuts at: 33 457 531 742 1667 2635 3185 3906 4591
Size: 424 74 211 925 968 550 721 685
Cuts at: 4591 5586 5777 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 968 925 721 685 550 424
211 191 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 898 898
Size: 6865
SfoI GGC'GCC
Cuts at: 4295 4543 4295
Size: 248 6617
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6865
SmaI CCC'GGG
Cuts at: 391 825 2457 2804 3036 3064 3346 3460 3885
Size: 434 1632 347 232 28 282 114 425
Cuts at: 3885 391
Size: 3371
Fragments arranged by size:
3371 1632 434 425 347 282 232 114
28
SmlI C'TyrA_G
Cuts at: 256 322 570 3266 3789 4018 4933 5427 5689
Size: 66 248 2696 523 229 915 494 262
Cuts at: 5689 256
Size: 1432
Fragments arranged by size:
2696 1432 915 523 494 262 248 229
66
SnaBI TAC'GTA
Cuts at: 3855 3855
Size: 6865
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6865
SphI G_CATG'C
Cuts at: 201 3916 4416 201
Size: 3715 500 2650
Fragments arranged by size:
3715 2650 500
SspI AAT'ATT
Cuts at: 2820 2820
Size: 6865
StuI AGG'CCT
Cuts at: 3057 3582 3057
Size: 525 6340
StyI C'CwwG_G
Cuts at: 125 549 635 1323 1640 1898 2723 3215 3605
Size: 424 86 688 317 258 825 492 390
Cuts at: 3605 3997 4351 125
Size: 392 354 2639
Fragments arranged by size:
2639 825 688 492 424 392 390 354
317 258 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1519 2461 2563 3462 4446 4579 5038 5249 1519
Size: 942 102 899 984 133 459 211 3135
Fragments arranged by size:
3135 984 942 899 459 211 133 102
TatI w'GTAC_w
Cuts at: 260 1775 2945 6380 260
Size: 1515 1170 3435 745
Fragments arranged by size:
3435 1515 1170 745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1228 1260 1828 1860 6382 6414 1228
Size: 32 568 32 4522 32 1679
Fragments arranged by size:
4522 1679 568 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1743 4660 1743
Size: 2917 3948
XbaI T'CTAG_A
Cuts at: 3894 3894
Size: 6865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1951 2274 2685 3213 4358 1951
Size: 323 411 528 1145 4458
Fragments arranged by size:
4458 1145 528 411 323
XhoI C'TCGA_G
Cuts at: 256 256
Size: 6865
XmaI C'CCGG_G
Cuts at: 389 823 2455 2802 3034 3062 3344 3458 3883
Size: 434 1632 347 232 28 282 114 425
Cuts at: 3883 389
Size: 3371
Fragments arranged by size:
3371 1632 434 425 347 282 232 114
28
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AvrII BaeI BfrBI BsiWI
BsmBI BsrGI BstEII BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NsiI PacI PmlI
PshAI PsiI PsrI SanDI SgrAI SrfI SwaI XmnI
ZraI