Restriction map for
pF1KSDB0003
(Circular) (Six-base) MAPSORT of: pF1KSDB0003.seq Check: 1701 from: 1 to: 4645
pF1KSDB0003 4645 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 575 575
Size: 4645
AatII G_ACGT'C
Cuts at: 824 824
Size: 4645
AccI GT'mk_AC
Cuts at: 1398 1681 1398
Size: 283 4362
Acc65I G'GTAC_C
Cuts at: 1659 1659
Size: 4645
AclI AA'CG_TT
Cuts at: 4485 4485
Size: 4645
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 2468 2900 3649 3917 1210
Size: 1258 432 749 268 1938
Fragments arranged by size:
1938 1258 749 432 268
AflII C'TTAA_G
Cuts at: 1188 1188
Size: 4645
AflIII A'CryG_T
Cuts at: 1995 3101 4078 1995
Size: 1106 977 2562
Fragments arranged by size:
2562 1106 977
AgeI A'CCGG_T
Cuts at: 3033 3033
Size: 4645
AleI CACnn'nnGTG
Cuts at: 684 684
Size: 4645
AlwNI CAG_nnn'CTG
Cuts at: 1104 1948 3517 1104
Size: 844 1569 2232
Fragments arranged by size:
2232 1569 844
ApaI G_GGCC'C
Cuts at: 1452 1452
Size: 4645
ApaLI G'TGCA_C
Cuts at: 3415 3415
Size: 4645
ApoI r'AATT_y
Cuts at: 1147 1312 1342 1647 3998 4254 1147
Size: 165 30 305 2351 256 1538
Fragments arranged by size:
2351 1538 305 256 165 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4645
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4645
AvaI C'yCGr_G
Cuts at: 207 1663 207
Size: 1456 3189
BamHI G'GATC_C
Cuts at: 1668 1668
Size: 4645
BanI G'GyrC_C
Cuts at: 224 472 1659 2073 2321 2356 224
Size: 248 1187 414 248 35 2513
Fragments arranged by size:
2513 1187 414 248 248 35
BanII G_rGCy'C
Cuts at: 1111 1452 1657 2687 1111
Size: 341 205 1030 3069
Fragments arranged by size:
3069 1030 341 205
BbeI G_GCGC'C
Cuts at: 2077 2325 2077
Size: 248 4397
BbsI GAAGACnn'nnnn_
Cuts at: 584 584
Size: 4645
BciVI GTATCCnnnnn_n'
Cuts at: 2541 3304 4645 2541
Size: 763 1341 2541
Fragments arranged by size:
2541 1341 763
BclI T'GATC_A
Cuts at: 263 2163 263
Size: 1900 2745
BfrBI ATG'CAT
Cuts at: 523 523
Size: 4645
BglII A'GATC_T
Cuts at: 2158 4197 2158
Size: 2039 2606
BlpI GC'TnA_GC
Cuts at: 1755 1755
Size: 4645
Bme1580I G_kGCm'C
Cuts at: 1452 2268 2361 3419 1452
Size: 816 93 1058 2678
Fragments arranged by size:
2678 1058 816 93
BmrI ACTGGGnnnn_n'
Cuts at: 1509 1855 1936 2271 4417 1509
Size: 346 81 335 2146 1737
Fragments arranged by size:
2146 1737 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3010 3010
Size: 4645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1216 1027
Size: 189 4456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1819 2698 3192 3490 1819
Size: 879 494 298 2974
Fragments arranged by size:
2974 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4349 4349
Size: 4645
BsaAI yAC'GTr
Cuts at: 1635 1996 1635
Size: 361 4284
BsaBI GATnn'nnATC
Cuts at: 6 2157 4196 6
Size: 2151 2039 455
Fragments arranged by size:
2151 2039 455
BsaHI Gr'CG_yC
Cuts at: 821 2074 2322 3026 4522 821
Size: 1253 248 704 1496 944
Fragments arranged by size:
1496 1253 944 704 248
BsaWI w'CCGG_w
Cuts at: 1832 2353 3033 3307 3454 3865 1832
Size: 521 680 274 147 411 2612
Fragments arranged by size:
2612 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 192 1624 192
Size: 1432 3213
BseYI C'CCAG_C
Cuts at: 153 1104 1488 2068 2826 3405 3901 153
Size: 951 384 580 758 579 496 897
Fragments arranged by size:
951 897 758 580 579 496 384
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 547
Size: 4645
BsiEI CG_ry'CG
Cuts at: 104 220 2185 2231 3441 104
Size: 116 1965 46 1210 1308
Fragments arranged by size:
1965 1308 1210 116 46
BsiHKAI G_wGCw'C
Cuts at: 1657 2435 2625 3419 1657
Size: 778 190 794 2883
Fragments arranged by size:
2883 794 778 190
BsmI GAATG_Cn'
Cuts at: 326 326
Size: 4645
Bsp1286I G_dGCh'C
Cuts at: 1111 1452 1657 2268 2361 2435 2625 2687 3419
Size: 341 205 611 93 74 190 62 732
Cuts at: 3419 1111
Size: 2337
Fragments arranged by size:
2337 732 611 341 205 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3865 3865
Size: 4645
BspHI T'CATG_A
Cuts at: 1412 3821 1412
Size: 2409 2236
BspMI ACCTGCnnnn'nnnn_
Cuts at: 575 1693 1907 2209 2590 575
Size: 1118 214 302 381 2630
Fragments arranged by size:
2630 1118 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1434 2935 2989 4475 4640 50
Size: 1384 1501 54 1486 165 55
Fragments arranged by size:
1501 1486 1384 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2555 2555
Size: 4645
BsrFI r'CCGG_y
Cuts at: 215 2641 2822 3033 215
Size: 2426 181 211 1827
Fragments arranged by size:
2426 1827 211 181
BssSI C'ACGA_G
Cuts at: 2914 3274 2914
Size: 360 4285
BstAPI GCAn_nnn'nTGC
Cuts at: 1221 2020 1221
Size: 799 3846
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4645
BstEII G'GTnAC_C
Cuts at: 738 738
Size: 4645
BstYI r'GATC_y
Cuts at: 1 1668 2135 2158 2493 2739 3753 3839 3851
Size: 1667 467 23 335 246 1014 86 12
Cuts at: 3851 4197 1
Size: 346 449
Fragments arranged by size:
1667 1014 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1399 1399
Size: 4645
BtgI C'CryG_G
Cuts at: 408 705 408
Size: 297 4348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2593 2774 2937 2593
Size: 181 163 4301
Fragments arranged by size:
4301 181 163
BtsI GCAGTG_nn'
Cuts at: 837 1294 1566 1886 837
Size: 457 272 320 3596
Fragments arranged by size:
3596 457 320 272
DraI TTT'AAA
Cuts at: 1642 1642
Size: 4645
DraIII CAC_nnn'GTG
Cuts at: 924 924
Size: 4645
DrdI GACnn_nn'nnGTC
Cuts at: 380 2349 3209 380
Size: 1969 860 1816
Fragments arranged by size:
1969 1816 860
EaeI y'GGCC_r
Cuts at: 217 2228 2402 2793 2820 4167 217
Size: 2011 174 391 27 1347 695
Fragments arranged by size:
2011 1347 695 391 174 27
EagI C'GGCC_G
Cuts at: 217 2228 217
Size: 2011 2634
EarI CTCTTCn'nnn_
Cuts at: 700 1441 1516 2666 2876 700
Size: 741 75 1150 210 2469
Fragments arranged by size:
2469 1150 741 210 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 424 2851 3163 3309 4455 424
Size: 2427 312 146 1146 614
Fragments arranged by size:
2427 1146 614 312 146
EcoICRI GAG'CTC
Cuts at: 1655 1655
Size: 4645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1210 1216 2468 2900 3649 3917 1027
Size: 183 6 1252 432 749 268 1755
Fragments arranged by size:
1755 1252 749 432 268 183 6
EcoO109I rG'GnC_Cy
Cuts at: 1782 1782
Size: 4645
EcoRI G'AATT_C
Cuts at: 1312 1647 1312
Size: 335 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 601 1348 2662 601
Size: 747 1314 2584
Fragments arranged by size:
2584 1314 747
FspI TGC'GCA
Cuts at: 2424 2424
Size: 4645
HaeII r_GCGC'y
Cuts at: 2077 2325 3349 4328 2077
Size: 248 1024 979 2394
Fragments arranged by size:
2394 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 743 775 2485 2515 2517 2547 743
Size: 32 1710 30 2 30 2841
Fragments arranged by size:
2841 1710 32 30 30 2
HincII GTy'rAC
Cuts at: 1682 1682
Size: 4645
HindIII A'AGCT_T
Cuts at: 501 859 501
Size: 358 4287
KasI G'GCGC_C
Cuts at: 2073 2321 2073
Size: 248 4397
KpnI G_GTAC'C
Cuts at: 1663 1663
Size: 4645
MfeI C'AATT_G
Cuts at: 1031 1031
Size: 4645
MluI A'CGCG_T
Cuts at: 4078 4078
Size: 4645
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1457 3316 3500 1457
Size: 1859 184 2602
Fragments arranged by size:
2602 1859 184
MscI TGG'CCA
Cuts at: 2404 2404
Size: 4645
MslI CAynn'nnrTG
Cuts at: 130 684 2632 2759 130
Size: 554 1948 127 2016
Fragments arranged by size:
2016 1948 554 127
MspA1I CmG'CkG
Cuts at: 240 1104 1568 1754 2068 2428 3443 3688 240
Size: 864 464 186 314 360 1015 245 1197
Fragments arranged by size:
1197 1015 864 464 360 314 245 186
NaeI GCC'GGC
Cuts at: 217 2824 217
Size: 2607 2038
NarI GG'CG_CC
Cuts at: 2074 2322 2074
Size: 248 4397
NcoI C'CATG_G
Cuts at: 705 705
Size: 4645
NdeI CA'TA_TG
Cuts at: 602 602
Size: 4645
NgoMIV G'CCGG_C
Cuts at: 215 2822 215
Size: 2607 2038
NruI TCG'CGA
Cuts at: 3006 3006
Size: 4645
NsiI A_TGCA'T
Cuts at: 525 525
Size: 4645
NspI r_CATG'y
Cuts at: 1041 1488 1696 2196 3105 1041
Size: 447 208 500 909 2581
Fragments arranged by size:
2581 909 500 447 208
PasI CC'CwG_GG
Cuts at: 245 934 245
Size: 689 3956
PciI A'CATG_T
Cuts at: 3101 3101
Size: 4645
PfoI T'CCnGG_A
Cuts at: 360 1439 360
Size: 1079 3566
PmeI GTTT'AAAC
Cuts at: 1642 1642
Size: 4645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3784 3816 3784
Size: 32 4613
PspOMI G'GGCC_C
Cuts at: 1448 1448
Size: 4645
PstI C_TGCA'G
Cuts at: 308 912 1690 2375 308
Size: 604 778 685 2578
Fragments arranged by size:
2578 778 685 604
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4645
PvuII CAG'CTG
Cuts at: 1104 2068 2428 1104
Size: 964 360 3321
Fragments arranged by size:
3321 964 360
RsrII CG'GwC_CG
Cuts at: 2838 2838
Size: 4645
SacI G_AGCT'C
Cuts at: 1657 1657
Size: 4645
SalI G'TCGA_C
Cuts at: 1680 1680
Size: 4645
SapI GCTCTTCn'nnn_
Cuts at: 1441 1516 2666 2876 1441
Size: 75 1150 210 3210
Fragments arranged by size:
3210 1150 210 75
SbfI CC_TGCA'GG
Cuts at: 1690 1690
Size: 4645
ScaI AGT'ACT
Cuts at: 4162 4162
Size: 4645
SfcI C'TryA_G
Cuts at: 33 304 908 1317 1686 2371 3366 3557 33
Size: 271 604 409 369 685 995 191 1121
Fragments arranged by size:
1121 995 685 604 409 369 271 191
SfoI GGC'GCC
Cuts at: 2075 2323 2075
Size: 248 4397
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4645
SmaI CCC'GGG
Cuts at: 209 1665 209
Size: 1456 3189
SmlI C'TyrA_G
Cuts at: 1188 1798 2713 3207 3469 1188
Size: 610 915 494 262 2364
Fragments arranged by size:
2364 915 610 494 262
SnaBI TAC'GTA
Cuts at: 1635 1635
Size: 4645
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4645
SphI G_CATG'C
Cuts at: 1041 1696 2196 1041
Size: 655 500 3490
Fragments arranged by size:
3490 655 500
StyI C'CwwG_G
Cuts at: 585 705 1593 1777 2131 585
Size: 120 888 184 354 3099
Fragments arranged by size:
3099 888 354 184 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 167 841 947 2226 2359 2818 3029 167
Size: 674 106 1279 133 459 211 1783
Fragments arranged by size:
1783 1279 674 459 211 133 106
TatI w'GTAC_w
Cuts at: 448 4160 448
Size: 3712 933
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4162 4194 4162
Size: 32 4613
Tth111I GACn'n_nGTC
Cuts at: 2440 2440
Size: 4645
XbaI T'CTAG_A
Cuts at: 1674 1674
Size: 4645
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2138 2138
Size: 4645
XmaI C'CCGG_G
Cuts at: 207 1663 207
Size: 1456 3189
ZraI GAC'GTC
Cuts at: 822 822
Size: 4645
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NsiI NspI PasI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI AscI AvrII BaeI BbvCI
BcgI BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI
BsrGI BssHII BstXI Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI NheI NotI PacI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI