Restriction map for
pF1KSDB0005
(Circular) (Six-base) MAPSORT of: pF1KSDB0005.seq Check: 1530 from: 1 to: 6952
pF1KSDB0005 6952 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 3446 3446
Size: 6952
AccI GT'mk_AC
Cuts at: 851 2972 3938 3988 851
Size: 2121 966 50 3815
Fragments arranged by size:
3815 2121 966 50
Acc65I G'GTAC_C
Cuts at: 3966 3966
Size: 6952
AclI AA'CG_TT
Cuts at: 6792 6792
Size: 6952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 2128 2275 2587 2610 3448 3529 4775 5207
Size: 1215 147 312 23 838 81 1246 432
Cuts at: 5207 5956 6224 913
Size: 749 268 1641
Fragments arranged by size:
1641 1246 1215 838 749 432 312 268
147 81 23
AfeI AGC'GCT
Cuts at: 235 283 235
Size: 48 6904
AflIII A'CryG_T
Cuts at: 2657 4302 5408 6385 2657
Size: 1645 1106 977 3224
Fragments arranged by size:
3224 1645 1106 977
AgeI A'CCGG_T
Cuts at: 5340 5340
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 3828 3828
Size: 6952
AleI CACnn'nnGTG
Cuts at: 1336 1336
Size: 6952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 214 2135 214
Size: 1921 5031
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1126 1158 2164 2196 1126
Size: 32 1006 32 5882
Fragments arranged by size:
5882 1006 32 32
AlwNI CAG_nnn'CTG
Cuts at: 228 1875 2253 2769 2887 3039 3153 3167 3426
Size: 1647 378 516 118 152 114 14 259
Cuts at: 3426 3861 4255 5824 228
Size: 435 394 1569 1356
Fragments arranged by size:
1647 1569 1356 516 435 394 378 259
152 118 114 14
ApaI G_GGCC'C
Cuts at: 1009 2292 1009
Size: 1283 5669
ApaLI G'TGCA_C
Cuts at: 2632 5722 2632
Size: 3090 3862
ApoI r'AATT_y
Cuts at: 3007 3954 6305 6561 3007
Size: 947 2351 256 3398
Fragments arranged by size:
3398 2351 947 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6952
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6952
AvaI C'yCGr_G
Cuts at: 395 463 508 526 628 786 1560 3532 3970
Size: 68 45 18 102 158 774 1972 438
Cuts at: 3970 395
Size: 3377
Fragments arranged by size:
3377 1972 774 438 158 102 68 45
18
BamHI G'GATC_C
Cuts at: 605 2019 3975 605
Size: 1414 1956 3582
Fragments arranged by size:
3582 1956 1414
BanI G'GyrC_C
Cuts at: 254 382 1298 1384 1469 2460 3324 3966 4380
Size: 128 916 86 85 991 864 642 414
Cuts at: 4380 4628 4663 254
Size: 248 35 2543
Fragments arranged by size:
2543 991 916 864 642 414 248 128
86 85 35
BanII G_rGCy'C
Cuts at: 185 395 464 787 1009 1569 1784 1817 2292
Size: 210 69 323 222 560 215 33 475
Cuts at: 2292 2852 3031 3539 3964 4994 185
Size: 560 179 508 425 1030 2143
Fragments arranged by size:
2143 1030 560 560 508 475 425 323
222 215 210 179 69 33
BbeI G_GCGC'C
Cuts at: 258 386 1388 4384 4632 258
Size: 128 1002 2996 248 2578
Fragments arranged by size:
2996 2578 1002 248 128
BbsI GAAGACnn'nnnn_
Cuts at: 2301 3270 2301
Size: 969 5983
BbvCI CC'TCA_GC
Cuts at: 1522 1522
Size: 6952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 521 555 957 991 1391 1425 3165 3199 521
Size: 34 402 34 400 34 1740 34 4274
Fragments arranged by size:
4274 1740 402 400 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1423 3038 4848 5611 6952 1423
Size: 1615 1810 763 1341 1423
Fragments arranged by size:
1810 1615 1423 1341 763
BclI T'GATC_A
Cuts at: 4470 4470
Size: 6952
BfrBI ATG'CAT
Cuts at: 802 802
Size: 6952
BglI GCCn_nnn'nGGC
Cuts at: 577 936 2645 577
Size: 359 1709 4884
Fragments arranged by size:
4884 1709 359
BglII A'GATC_T
Cuts at: 1361 2495 4465 6504 1361
Size: 1134 1970 2039 1809
Fragments arranged by size:
2039 1970 1809 1134
BlpI GC'TnA_GC
Cuts at: 2987 4062 2987
Size: 1075 5877
Bme1580I G_kGCm'C
Cuts at: 1009 2292 2447 2465 2636 3329 4575 4668 5726
Size: 1283 155 18 171 693 1246 93 1058
Cuts at: 5726 1009
Size: 2235
Fragments arranged by size:
2235 1283 1246 1058 693 171 155 93
18
BmgBI CAC'GTC
Cuts at: 2307 3486 2307
Size: 1179 5773
BmrI ACTGGGnnnn_n'
Cuts at: 4162 4243 4578 6724 4162
Size: 81 335 2146 4390
Fragments arranged by size:
4390 2146 335 81
BmtI G_CTAG'C
Cuts at: 1652 1652
Size: 6952
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 219 1176 5317 219
Size: 957 4141 1854
Fragments arranged by size:
4141 1854 957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 280 967 1128 1591 1696 2455 2776 2944
Size: 30 687 161 463 105 759 321 168
Cuts at: 2944 3043 3901 250
Size: 99 858 3301
Fragments arranged by size:
3301 858 759 687 463 321 168 161
105 99 30
Bpu10I CC'TnA_GC
Cuts at: 586 1522 1666 1778 586
Size: 936 144 112 5760
Fragments arranged by size:
5760 936 144 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2329 4126 5005 5499 5797 2329
Size: 1797 879 494 298 3484
Fragments arranged by size:
3484 1797 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1563 1750 6656 1563
Size: 187 4906 1859
Fragments arranged by size:
4906 1859 187
BsaAI yAC'GTr
Cuts at: 657 3942 4303 657
Size: 3285 361 3306
Fragments arranged by size:
3306 3285 361
BsaBI GATnn'nnATC
Cuts at: 6 2494 4464 6503 6
Size: 2488 1970 2039 455
Fragments arranged by size:
2488 2039 1970 455
BsaHI Gr'CG_yC
Cuts at: 255 383 638 848 869 911 1343 1385 2153
Size: 128 255 210 21 42 432 42 768
Cuts at: 2153 3443 4381 4629 5333 6829 255
Size: 1290 938 248 704 1496 378
Fragments arranged by size:
1496 1290 938 768 704 432 378 255
248 210 128 42 42 21
BsaWI w'CCGG_w
Cuts at: 3400 4139 4660 5340 5614 5761 6172 3400
Size: 739 521 680 274 147 411 4180
Fragments arranged by size:
4180 739 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 370 400 1108 1126 1138 1156 1489 1519 2575
Size: 30 708 18 12 18 333 30 1056
Cuts at: 2575 2605 3829 3859 370
Size: 30 1224 30 3463
Fragments arranged by size:
3463 1224 1056 708 333 30 30 30
30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 472 819 1152 1165 1471 1497 1669 2094 2209
Size: 347 333 13 306 26 172 425 115
Cuts at: 2209 2485 2661 3367 3391 472
Size: 276 176 706 24 4033
Fragments arranged by size:
4033 706 425 347 333 306 276 176
172 115 26 24 13
BseYI C'CCAG_C
Cuts at: 770 1887 2614 3799 4375 5133 5712 6208 770
Size: 1117 727 1185 576 758 579 496 1514
Fragments arranged by size:
1514 1185 1117 758 727 579 576 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 2319 2708 2901 3709 1849
Size: 470 389 193 808 5092
Fragments arranged by size:
5092 808 470 389 193
BsiEI CG_ry'CG
Cuts at: 104 558 4492 4538 5748 104
Size: 454 3934 46 1210 1308
Fragments arranged by size:
3934 1308 1210 454 46
BsiHKAI G_wGCw'C
Cuts at: 1030 1784 2086 2243 2636 3106 3964 4742 4932
Size: 754 302 157 393 470 858 778 190
Cuts at: 4932 5726 1030
Size: 794 2256
Fragments arranged by size:
2256 858 794 778 754 470 393 302
190 157
BsmI GAATG_Cn'
Cuts at: 2807 2807
Size: 6952
BsmBI CGTCTCn'nnnn_
Cuts at: 1391 1391
Size: 6952
Bsp1286I G_dGCh'C
Cuts at: 185 395 464 787 1009 1030 1569 1784 1817
Size: 210 69 323 222 21 539 215 33
Cuts at: 1817 2086 2243 2292 2447 2465 2636 2852 3031
Size: 269 157 49 155 18 171 216 179
Cuts at: 3031 3106 3329 3539 3964 4575 4668 4742 4932
Size: 75 223 210 425 611 93 74 190
Cuts at: 4932 4994 5726 185
Size: 62 732 1411
Fragments arranged by size:
1411 732 611 539 425 323 269 223
222 216 215 210 210 190 179 171
157 155 93 75 74 69 62 49
33 21 18
BspEI T'CCGG_A
Cuts at: 6172 6172
Size: 6952
BspHI T'CATG_A
Cuts at: 6128 6128
Size: 6952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 308 1055 2333 3632 3659 4000 4214 4516 4897
Size: 747 1278 1299 27 341 214 302 381
Cuts at: 4897 308
Size: 2363
Fragments arranged by size:
2363 1299 1278 747 381 341 302 214
27
BsrBI CCG'CTC
Cuts at: 50 603 1011 1229 2357 2400 5242 5296 6782
Size: 553 408 218 1128 43 2842 54 1486
Cuts at: 6782 6947 50
Size: 165 55
Fragments arranged by size:
2842 1486 1128 553 408 218 165 55
54 43
BsrDI GCAATG_nn'
Cuts at: 4862 4862
Size: 6952
BsrFI r'CCGG_y
Cuts at: 338 1301 1466 1731 2243 4948 5129 5340 338
Size: 963 165 265 512 2705 181 211 1950
Fragments arranged by size:
2705 1950 963 512 265 211 181 165
BssHII G'CGCG_C
Cuts at: 360 360
Size: 6952
BssSI C'ACGA_G
Cuts at: 676 2778 5221 5581 676
Size: 2102 2443 360 2047
Fragments arranged by size:
2443 2102 2047 360
BstAPI GCAn_nnn'nTGC
Cuts at: 309 3153 4327 309
Size: 2844 1174 2934
Fragments arranged by size:
2934 2844 1174
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6952
BstEII G'GTnAC_C
Cuts at: 3300 3300
Size: 6952
BstXI CCAn_nnnn'nTGG
Cuts at: 753 1338 1887 1935 753
Size: 585 549 48 5770
Fragments arranged by size:
5770 585 549 48
BstYI r'GATC_y
Cuts at: 1 605 1361 1790 2019 2406 2495 3080 3975
Size: 604 756 429 229 387 89 585 895
Cuts at: 3975 4442 4465 4800 5046 6060 6146 6158 6504
Size: 467 23 335 246 1014 86 12 346
Cuts at: 6504 1
Size: 449
Fragments arranged by size:
1014 895 756 604 585 467 449 429
387 346 335 246 229 89 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 3040 3040
Size: 6952
BtgI C'CryG_G
Cuts at: 125 618 1392 1928 2753 3410 3737 125
Size: 493 774 536 825 657 327 3340
Fragments arranged by size:
3340 825 774 657 536 493 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 360 1496 3168 3480 3564 4900 5081 5244 360
Size: 1136 1672 312 84 1336 181 163 2068
Fragments arranged by size:
2068 1672 1336 1136 312 181 163 84
BtsI GCAGTG_nn'
Cuts at: 1291 1305 1681 1840 2447 2593 4193 1291
Size: 14 376 159 607 146 1600 4050
Fragments arranged by size:
4050 1600 607 376 159 146 14
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3726 3761 3726
Size: 35 6917
DraI TTT'AAA
Cuts at: 3949 3949
Size: 6952
DrdI GACnn_nn'nnGTC
Cuts at: 4656 5516 4656
Size: 860 6092
EaeI y'GGCC_r
Cuts at: 314 621 937 1167 1877 3407 4535 4709 5100
Size: 307 316 230 710 1530 1128 174 391
Cuts at: 5100 5127 6474 314
Size: 27 1347 792
Fragments arranged by size:
1530 1347 1128 792 710 391 316 307
230 174 27
EagI C'GGCC_G
Cuts at: 4535 4535
Size: 6952
EarI CTCTTCn'nnn_
Cuts at: 322 1822 1870 2524 3241 3547 4973 5183 322
Size: 1500 48 654 717 306 1426 210 2091
Fragments arranged by size:
2091 1500 1426 717 654 306 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 649 5158 5470 5616 6762 649
Size: 4509 312 146 1146 839
Fragments arranged by size:
4509 1146 839 312 146
EcoICRI GAG'CTC
Cuts at: 1782 3962 1782
Size: 2180 4772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 280 913 967 1128 1591 1696 2128 2275
Size: 30 633 54 161 463 105 432 147
Cuts at: 2275 2455 2587 2610 2776 2944 3043 3448 3529
Size: 180 132 23 166 168 99 405 81
Cuts at: 3529 3901 4775 5207 5956 6224 250
Size: 372 874 432 749 268 978
Fragments arranged by size:
978 874 749 633 463 432 432 405
372 268 180 168 166 161 147 132
105 99 81 54 30 23
EcoNI CCTnn'n_nnAGG
Cuts at: 262 691 3640 262
Size: 429 2949 3574
Fragments arranged by size:
3574 2949 429
EcoO109I rG'GnC_Cy
Cuts at: 372 562 809 929 1005 1067 1629 2288 3150
Size: 190 247 120 76 62 562 659 862
Cuts at: 3150 4089 372
Size: 939 3235
Fragments arranged by size:
3235 939 862 659 562 247 190 120
76 62
EcoRI G'AATT_C
Cuts at: 3954 3954
Size: 6952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2280 3453 3534 4969 2280
Size: 1173 81 1435 4263
Fragments arranged by size:
4263 1435 1173 81
FspI TGC'GCA
Cuts at: 1018 4731 1018
Size: 3713 3239
HaeII r_GCGC'y
Cuts at: 237 258 285 386 427 586 1050 1388 1467
Size: 21 27 101 41 159 464 338 79
Cuts at: 1467 3366 4384 4632 5656 6635 237
Size: 1899 1018 248 1024 979 554
Fragments arranged by size:
1899 1024 1018 979 554 464 338 248
159 101 79 41 27 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 370 402 1108 1140 1147 1179 1489 1521 2575
Size: 32 706 32 7 32 310 32 1054
Cuts at: 2575 2607 2824 2856 2892 2924 3814 3829 3846
Size: 32 217 32 36 32 890 15 17
Cuts at: 3846 3861 3902 3934 4792 4822 4824 4854 370
Size: 15 41 32 858 30 2 30 2468
Fragments arranged by size:
2468 1054 890 858 706 310 217 41
36 32 32 32 32 32 32 32
32 30 30 17 15 15 7 2
HincII GTy'rAC
Cuts at: 699 852 3989 699
Size: 153 3137 3662
Fragments arranged by size:
3662 3137 153
HindIII A'AGCT_T
Cuts at: 2736 2736
Size: 6952
KasI G'GCGC_C
Cuts at: 254 382 1384 4380 4628 254
Size: 128 1002 2996 248 2578
Fragments arranged by size:
2996 2578 1002 248 128
KpnI G_GTAC'C
Cuts at: 3970 3970
Size: 6952
MluI A'CGCG_T
Cuts at: 6385 6385
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 695 2047 2561 5623 5807 617
Size: 78 1352 514 3062 184 1762
Fragments arranged by size:
3062 1762 1352 514 184 78
MscI TGG'CCA
Cuts at: 316 623 1169 1879 3409 4711 316
Size: 307 546 710 1530 1302 2557
Fragments arranged by size:
2557 1530 1302 710 546 307
MslI CAynn'nnrTG
Cuts at: 751 1336 1475 1933 2224 3187 4939 5066 751
Size: 585 139 458 291 963 1752 127 2637
Fragments arranged by size:
2637 1752 963 585 458 291 139 127
MspA1I CmG'CkG
Cuts at: 842 1714 1851 1887 2250 2814 2986 3138 3827
Size: 872 137 36 363 564 172 152 689
Cuts at: 3827 3879 4061 4375 4735 5750 5995 842
Size: 52 182 314 360 1015 245 1799
Fragments arranged by size:
1799 1015 872 689 564 363 360 314
245 182 172 152 137 52 36
NaeI GCC'GGC
Cuts at: 1468 5131 1468
Size: 3663 3289
NarI GG'CG_CC
Cuts at: 255 383 1385 4381 4629 255
Size: 128 1002 2996 248 2578
Fragments arranged by size:
2996 2578 1002 248 128
NcoI C'CATG_G
Cuts at: 125 618 1392 1928 125
Size: 493 774 536 5149
Fragments arranged by size:
5149 774 536 493
NgoMIV G'CCGG_C
Cuts at: 1466 5129 1466
Size: 3663 3289
NheI G'CTAG_C
Cuts at: 1648 1648
Size: 6952
NruI TCG'CGA
Cuts at: 5313 5313
Size: 6952
NsiI A_TGCA'T
Cuts at: 804 804
Size: 6952
NspI r_CATG'y
Cuts at: 1212 3618 4003 4503 5412 1212
Size: 2406 385 500 909 2752
Fragments arranged by size:
2752 2406 909 500 385
PasI CC'CwG_GG
Cuts at: 732 732
Size: 6952
PciI A'CATG_T
Cuts at: 5408 5408
Size: 6952
PflMI CCAn_nnn'nTGG
Cuts at: 1875 2424 3348 1875
Size: 549 924 5479
Fragments arranged by size:
5479 924 549
PfoI T'CCnGG_A
Cuts at: 170 1214 2708 170
Size: 1044 1494 4414
Fragments arranged by size:
4414 1494 1044
PmeI GTTT'AAAC
Cuts at: 3949 3949
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1126 1158 6091 6123 1126
Size: 32 4933 32 1955
Fragments arranged by size:
4933 1955 32 32
PpuMI rG'GwC_Cy
Cuts at: 562 1067 1629 562
Size: 505 562 5885
Fragments arranged by size:
5885 562 505
PshAI GACnn'nnGTC
Cuts at: 2214 2968 3274 2214
Size: 754 306 5892
Fragments arranged by size:
5892 754 306
PspOMI G'GGCC_C
Cuts at: 1005 2288 1005
Size: 1283 5669
PspXI vC'TCGA_Gb
Cuts at: 786 786
Size: 6952
PstI C_TGCA'G
Cuts at: 305 1123 1446 2150 2765 3158 3878 3997 4682
Size: 818 323 704 615 393 720 119 685
Cuts at: 4682 305
Size: 2575
Fragments arranged by size:
2575 818 720 704 685 615 393 323
119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6952
PvuII CAG'CTG
Cuts at: 842 1887 2250 2986 3138 3827 3879 4375 4735
Size: 1045 363 736 152 689 52 496 360
Cuts at: 4735 842
Size: 3059
Fragments arranged by size:
3059 1045 736 689 496 363 360 152
52
RsrII CG'GwC_CG
Cuts at: 717 5145 717
Size: 4428 2524
SacI G_AGCT'C
Cuts at: 1784 3964 1784
Size: 2180 4772
SalI G'TCGA_C
Cuts at: 850 3987 850
Size: 3137 3815
SanDI GG'GwC_CC
Cuts at: 562 562
Size: 6952
SapI GCTCTTCn'nnn_
Cuts at: 322 1822 4973 5183 322
Size: 1500 3151 210 2091
Fragments arranged by size:
3151 2091 1500 210
SbfI CC_TGCA'GG
Cuts at: 3158 3997 3158
Size: 839 6113
ScaI AGT'ACT
Cuts at: 6469 6469
Size: 6952
SfcI C'TryA_G
Cuts at: 33 301 1119 1442 2146 2761 2973 3154 3874
Size: 268 818 323 704 615 212 181 720
Cuts at: 3874 3993 4678 5673 5864 33
Size: 119 685 995 191 1121
Fragments arranged by size:
1121 995 818 720 704 685 615 323
268 212 191 181 119
SfiI GGCCn_nnn'nGGCC
Cuts at: 936 936
Size: 6952
SfoI GGC'GCC
Cuts at: 256 384 1386 4382 4630 256
Size: 128 1002 2996 248 2578
Fragments arranged by size:
2996 2578 1002 248 128
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6952
SmaI CCC'GGG
Cuts at: 510 528 630 3972 510
Size: 18 102 3342 3490
Fragments arranged by size:
3490 3342 102 18
SmlI C'TyrA_G
Cuts at: 786 2344 4105 5020 5514 5776 786
Size: 1558 1761 915 494 262 1962
Fragments arranged by size:
1962 1761 1558 915 494 262
SnaBI TAC'GTA
Cuts at: 3942 3942
Size: 6952
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6952
SphI G_CATG'C
Cuts at: 3618 4003 4503 3618
Size: 385 500 6067
Fragments arranged by size:
6067 500 385
StuI AGG'CCT
Cuts at: 244 244
Size: 6952
StyI C'CwwG_G
Cuts at: 125 317 618 934 1392 1928 2015 3086 3320
Size: 192 301 316 458 536 87 1071 234
Cuts at: 3320 3833 4084 4438 125
Size: 513 251 354 2639
Fragments arranged by size:
2639 1071 536 513 458 354 316 301
251 234 192 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1879 3204 4533 4666 5125 5336 1879
Size: 1325 1329 133 459 211 3495
Fragments arranged by size:
3495 1329 1325 459 211 133
TatI w'GTAC_w
Cuts at: 2912 3515 3866 6467 2912
Size: 603 351 2601 3397
Fragments arranged by size:
3397 2601 603 351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 6469 6501 2159
Size: 32 4278 32 2610
Fragments arranged by size:
4278 2610 32 32
Tth111I GACn'n_nGTC
Cuts at: 4747 4747
Size: 6952
XbaI T'CTAG_A
Cuts at: 3981 3981
Size: 6952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 2037 2454 2884 4445 255
Size: 1782 417 430 1561 2762
Fragments arranged by size:
2762 1782 1561 430 417
XhoI C'TCGA_G
Cuts at: 786 786
Size: 6952
XmaI C'CCGG_G
Cuts at: 508 526 628 3970 508
Size: 18 102 3342 3490
Fragments arranged by size:
3490 3342 102 18
XmnI GAAnn'nnTTC
Cuts at: 649 2728 649
Size: 2079 4873
ZraI GAC'GTC
Cuts at: 3444 3444
Size: 6952
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AscI AvrII BaeI BsiWI BsrGI BstZ17I
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NotI PacI PmlI PsiI PsrI SacII SexAI SgrAI
SrfI SspI SwaI