Restriction map for
pF1KSDB0008
(Circular) (Six-base) MAPSORT of: pF1KSDB0008.seq Check: 5013 from: 1 to: 5032
pF1KSDB0008 5032 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 2068 2068
Size: 5032
Acc65I G'GTAC_C
Cuts at: 1447 2046 1447
Size: 599 4433
AclI AA'CG_TT
Cuts at: 4872 4872
Size: 5032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 1052 1216 2855 3287 4036 4304 890
Size: 162 164 1639 432 749 268 1618
Fragments arranged by size:
1639 1618 749 432 268 164 162
AflIII A'CryG_T
Cuts at: 2382 3488 4465 2382
Size: 1106 977 2949
Fragments arranged by size:
2949 1106 977
AgeI A'CCGG_T
Cuts at: 3420 3420
Size: 5032
AhdI GACnn_n'nnGTC
Cuts at: 575 575
Size: 5032
AlwNI CAG_nnn'CTG
Cuts at: 492 891 1281 2335 3904 492
Size: 399 390 1054 1569 1620
Fragments arranged by size:
1620 1569 1054 399 390
ApaLI G'TGCA_C
Cuts at: 1657 3802 1657
Size: 2145 2887
ApoI r'AATT_y
Cuts at: 212 1330 2034 4385 4641 212
Size: 1118 704 2351 256 603
Fragments arranged by size:
2351 1118 704 603 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5032
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5032
AvaI C'yCGr_G
Cuts at: 1237 1790 2050 1237
Size: 553 260 4219
Fragments arranged by size:
4219 553 260
BamHI G'GATC_C
Cuts at: 2055 2055
Size: 5032
BanI G'GyrC_C
Cuts at: 1447 2046 2460 2708 2743 1447
Size: 599 414 248 35 3736
Fragments arranged by size:
3736 599 414 248 35
BanII G_rGCy'C
Cuts at: 224 440 2044 3074 224
Size: 216 1604 1030 2182
Fragments arranged by size:
2182 1604 1030 216
BbeI G_GCGC'C
Cuts at: 2464 2712 2464
Size: 248 4784
BbsI GAAGACnn'nnnn_
Cuts at: 574 1204 1484 574
Size: 630 280 4122
Fragments arranged by size:
4122 630 280
BbvCI CC'TCA_GC
Cuts at: 785 785
Size: 5032
BciVI GTATCCnnnnn_n'
Cuts at: 564 1279 2928 3691 5032 564
Size: 715 1649 763 1341 564
Fragments arranged by size:
1649 1341 763 715 564
BclI T'GATC_A
Cuts at: 2550 2550
Size: 5032
BglI GCCn_nnn'nGGC
Cuts at: 141 141
Size: 5032
BglII A'GATC_T
Cuts at: 2545 4584 2545
Size: 2039 2993
BlpI GC'TnA_GC
Cuts at: 2142 2142
Size: 5032
Bme1580I G_kGCm'C
Cuts at: 1661 2655 2748 3806 1661
Size: 994 93 1058 2887
Fragments arranged by size:
2887 1058 994 93
BmrI ACTGGGnnnn_n'
Cuts at: 552 2242 2323 2658 4804 552
Size: 1690 81 335 2146 780
Fragments arranged by size:
2146 1690 780 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1779 3397 1779
Size: 1618 3414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 454 1413 238
Size: 216 959 3857
Fragments arranged by size:
3857 959 216
Bpu10I CC'TnA_GC
Cuts at: 785 785
Size: 5032
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2206 3085 3579 3877 2206
Size: 879 494 298 3361
Fragments arranged by size:
3361 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 163 1883 4736 163
Size: 1720 2853 459
Fragments arranged by size:
2853 1720 459
BsaAI yAC'GTr
Cuts at: 1408 2022 2383 1408
Size: 614 361 4057
Fragments arranged by size:
4057 614 361
BsaBI GATnn'nnATC
Cuts at: 6 2544 4583 6
Size: 2538 2039 455
Fragments arranged by size:
2538 2039 455
BsaHI Gr'CG_yC
Cuts at: 2461 2709 3413 4909 2461
Size: 248 704 1496 2584
Fragments arranged by size:
2584 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2219 2740 3420 3694 3841 4252 2219
Size: 521 680 274 147 411 2999
Fragments arranged by size:
2999 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 706 937 1366 1981 706
Size: 231 429 615 3757
Fragments arranged by size:
3757 615 429 231
BseYI C'CCAG_C
Cuts at: 235 1061 2455 3213 3792 4288 235
Size: 826 1394 758 579 496 979
Fragments arranged by size:
1394 979 826 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 2572 2618 3828 104
Size: 2468 46 1210 1308
Fragments arranged by size:
2468 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 224 1290 1404 1661 2044 2822 3012 3806 224
Size: 1066 114 257 383 778 190 794 1450
Fragments arranged by size:
1450 1066 794 778 383 257 190 114
BsmBI CGTCTCn'nnnn_
Cuts at: 937 937
Size: 5032
Bsp1286I G_dGCh'C
Cuts at: 224 440 1290 1404 1661 2044 2655 2748 2822
Size: 216 850 114 257 383 611 93 74
Cuts at: 2822 3012 3074 3806 224
Size: 190 62 732 1450
Fragments arranged by size:
1450 850 732 611 383 257 216 190
114 93 74 62
BspEI T'CCGG_A
Cuts at: 4252 4252
Size: 5032
BspHI T'CATG_A
Cuts at: 1470 1752 4208 1470
Size: 282 2456 2294
Fragments arranged by size:
2456 2294 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2080 2294 2596 2977 2080
Size: 214 302 381 4135
Fragments arranged by size:
4135 381 302 214
BsrBI CCG'CTC
Cuts at: 50 542 3322 3376 4862 5027 50
Size: 492 2780 54 1486 165 55
Fragments arranged by size:
2780 1486 492 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1469 2942 1469
Size: 1473 3559
BsrFI r'CCGG_y
Cuts at: 3028 3209 3420 3028
Size: 181 211 4640
Fragments arranged by size:
4640 211 181
BsrGI T'GTAC_A
Cuts at: 650 650
Size: 5032
BssSI C'ACGA_G
Cuts at: 844 3301 3661 844
Size: 2457 360 2215
Fragments arranged by size:
2457 2215 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2407 2407
Size: 5032
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5032
BstYI r'GATC_y
Cuts at: 1 446 2055 2522 2545 2880 3126 4140 4226
Size: 445 1609 467 23 335 246 1014 86
Cuts at: 4226 4238 4584 1
Size: 12 346 449
Fragments arranged by size:
1609 1014 467 449 445 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 584 584
Size: 5032
BtgI C'CryG_G
Cuts at: 125 947 1772 125
Size: 822 825 3385
Fragments arranged by size:
3385 825 822
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1054 2980 3161 3324 1054
Size: 1926 181 163 2762
Fragments arranged by size:
2762 1926 181 163
BtsI GCAGTG_nn'
Cuts at: 1661 2273 1661
Size: 612 4420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 929 964 929
Size: 35 4997
DraI TTT'AAA
Cuts at: 1335 2029 1335
Size: 694 4338
DraIII CAC_nnn'GTG
Cuts at: 1408 1408
Size: 5032
DrdI GACnn_nn'nnGTC
Cuts at: 2736 3596 2736
Size: 860 4172
EaeI y'GGCC_r
Cuts at: 2615 2789 3180 3207 4554 2615
Size: 174 391 27 1347 3093
Fragments arranged by size:
3093 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2615 2615
Size: 5032
EarI CTCTTCn'nnn_
Cuts at: 515 866 1121 1615 1881 3053 3263 515
Size: 351 255 494 266 1172 210 2284
Fragments arranged by size:
2284 1172 494 351 266 255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3238 3550 3696 4842 3238
Size: 312 146 1146 3428
Fragments arranged by size:
3428 1146 312 146
EcoICRI GAG'CTC
Cuts at: 222 2042 222
Size: 1820 3212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 454 890 1052 1216 1413 2855 3287 4036
Size: 216 436 162 164 197 1442 432 749
Cuts at: 4036 4304 238
Size: 268 966
Fragments arranged by size:
1442 966 749 436 432 268 216 197
164 162
EcoO109I rG'GnC_Cy
Cuts at: 2169 2169
Size: 5032
EcoRI G'AATT_C
Cuts at: 2034 2034
Size: 5032
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 874 1531 1695 3049 874
Size: 657 164 1354 2857
Fragments arranged by size:
2857 1354 657 164
FspI TGC'GCA
Cuts at: 1106 2811 1106
Size: 1705 3327
HaeII r_GCGC'y
Cuts at: 2464 2712 3736 4715 2464
Size: 248 1024 979 2781
Fragments arranged by size:
2781 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 561 593 694 726 1000 1032 1104 1136 2872
Size: 32 101 32 274 32 72 32 1736
Cuts at: 2872 2902 2904 2934 561
Size: 30 2 30 2659
Fragments arranged by size:
2659 1736 274 101 72 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 206 2069 206
Size: 1863 3169
KasI G'GCGC_C
Cuts at: 2460 2708 2460
Size: 248 4784
KpnI G_GTAC'C
Cuts at: 1451 2050 1451
Size: 599 4433
MluI A'CGCG_T
Cuts at: 4465 4465
Size: 5032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 643 3703 3887 643
Size: 3060 184 1788
Fragments arranged by size:
3060 1788 184
MscI TGG'CCA
Cuts at: 2791 2791
Size: 5032
MslI CAynn'nnrTG
Cuts at: 3019 3146 3019
Size: 127 4905
MspA1I CmG'CkG
Cuts at: 235 432 868 1030 2141 2455 2815 3830 4075
Size: 197 436 162 1111 314 360 1015 245
Cuts at: 4075 235
Size: 1192
Fragments arranged by size:
1192 1111 1015 436 360 314 245 197
162
NaeI GCC'GGC
Cuts at: 3211 3211
Size: 5032
NarI GG'CG_CC
Cuts at: 2461 2709 2461
Size: 248 4784
NcoI C'CATG_G
Cuts at: 125 1772 125
Size: 1647 3385
NgoMIV G'CCGG_C
Cuts at: 3209 3209
Size: 5032
NruI TCG'CGA
Cuts at: 3393 3393
Size: 5032
NspI r_CATG'y
Cuts at: 2083 2583 3492 2083
Size: 500 909 3623
Fragments arranged by size:
3623 909 500
PasI CC'CwG_GG
Cuts at: 137 137
Size: 5032
PciI A'CATG_T
Cuts at: 3488 3488
Size: 5032
PflMI CCAn_nnn'nTGG
Cuts at: 456 960 1911 456
Size: 504 951 3577
Fragments arranged by size:
3577 951 504
PfoI T'CCnGG_A
Cuts at: 161 161
Size: 5032
PmeI GTTT'AAAC
Cuts at: 2029 2029
Size: 5032
PmlI CAC'GTG
Cuts at: 1408 1408
Size: 5032
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4171 4203 4171
Size: 32 5000
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1891 1923 1891
Size: 32 5000
PstI C_TGCA'G
Cuts at: 2077 2762 2077
Size: 685 4347
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5032
PvuII CAG'CTG
Cuts at: 235 432 868 1030 2455 2815 235
Size: 197 436 162 1425 360 2452
Fragments arranged by size:
2452 1425 436 360 197 162
RsrII CG'GwC_CG
Cuts at: 3225 3225
Size: 5032
SacI G_AGCT'C
Cuts at: 224 2044 224
Size: 1820 3212
SalI G'TCGA_C
Cuts at: 2067 2067
Size: 5032
SapI GCTCTTCn'nnn_
Cuts at: 866 1121 3053 3263 866
Size: 255 1932 210 2635
Fragments arranged by size:
2635 1932 255 210
SbfI CC_TGCA'GG
Cuts at: 2077 2077
Size: 5032
ScaI AGT'ACT
Cuts at: 1909 4549 1909
Size: 2640 2392
SfcI C'TryA_G
Cuts at: 33 506 824 1273 1366 1534 2073 2758 3753
Size: 473 318 449 93 168 539 685 995
Cuts at: 3753 3944 33
Size: 191 1121
Fragments arranged by size:
1121 995 685 539 473 449 318 191
168 93
SfoI GGC'GCC
Cuts at: 2462 2710 2462
Size: 248 4784
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5032
SmaI CCC'GGG
Cuts at: 1792 2052 1792
Size: 260 4772
SmlI C'TyrA_G
Cuts at: 1237 2185 3100 3594 3856 1237
Size: 948 915 494 262 2413
Fragments arranged by size:
2413 948 915 494 262
SnaBI TAC'GTA
Cuts at: 2022 2022
Size: 5032
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5032
SphI G_CATG'C
Cuts at: 2083 2583 2083
Size: 500 4532
StuI AGG'CCT
Cuts at: 2014 2014
Size: 5032
StyI C'CwwG_G
Cuts at: 125 409 1772 1997 2164 2518 125
Size: 284 1363 225 167 354 2639
Fragments arranged by size:
2639 1363 354 284 225 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 390 517 2613 2746 3205 3416 390
Size: 127 2096 133 459 211 2006
Fragments arranged by size:
2096 2006 459 211 133 127
TatI w'GTAC_w
Cuts at: 650 1907 4547 650
Size: 1257 2640 1135
Fragments arranged by size:
2640 1257 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4549 4581 4549
Size: 32 5000
Tth111I GACn'n_nGTC
Cuts at: 2827 2827
Size: 5032
XbaI T'CTAG_A
Cuts at: 2061 2061
Size: 5032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2525 2525
Size: 5032
XhoI C'TCGA_G
Cuts at: 1237 1237
Size: 5032
XmaI C'CCGG_G
Cuts at: 1790 2050 1790
Size: 260 4772
XmnI GAAnn'nnTTC
Cuts at: 1957 1957
Size: 5032
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BssHII BstEII BstXI BstZ17I ClaI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI ZraI