Restriction map for
pF1KSDB0010
(Circular) (Six-base) MAPSORT of: pF1KSDB0010.seq Check: 2733 from: 1 to: 4996
pF1KSDB0010 4996 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1463 2032 1463
Size: 569 4427
Acc65I G'GTAC_C
Cuts at: 256 2010 256
Size: 1754 3242
AclI AA'CG_TT
Cuts at: 4836 4836
Size: 4996
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 1395 2819 3251 4000 4268 677
Size: 718 1424 432 749 268 1405
Fragments arranged by size:
1424 1405 749 718 432 268
AfeI AGC'GCT
Cuts at: 1237 1570 1237
Size: 333 4663
AflIII A'CryG_T
Cuts at: 2346 3452 4429 2346
Size: 1106 977 2913
Fragments arranged by size:
2913 1106 977
AgeI A'CCGG_T
Cuts at: 3384 3384
Size: 4996
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 299
Size: 32 4964
AlwNI CAG_nnn'CTG
Cuts at: 271 1074 1143 1594 2299 3868 271
Size: 803 69 451 705 1569 1399
Fragments arranged by size:
1569 1399 803 705 451 69
ApaI G_GGCC'C
Cuts at: 227 464 634 227
Size: 237 170 4589
Fragments arranged by size:
4589 237 170
ApaLI G'TGCA_C
Cuts at: 3766 3766
Size: 4996
ApoI r'AATT_y
Cuts at: 1754 1998 4349 4605 1754
Size: 244 2351 256 2145
Fragments arranged by size:
2351 2145 256 244
AseI AT'TA_AT
Cuts at: 20 20
Size: 4996
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4996
AvaI C'yCGr_G
Cuts at: 829 1364 2014 829
Size: 535 650 3811
Fragments arranged by size:
3811 650 535
BamHI G'GATC_C
Cuts at: 1562 2019 1562
Size: 457 4539
BanI G'GyrC_C
Cuts at: 256 890 1079 2010 2424 2672 2707 256
Size: 634 189 931 414 248 35 2545
Fragments arranged by size:
2545 931 634 414 248 189 35
BanII G_rGCy'C
Cuts at: 227 330 464 634 1064 1747 1854 2008 3038
Size: 103 134 170 430 683 107 154 1030
Cuts at: 3038 227
Size: 2185
Fragments arranged by size:
2185 1030 683 430 170 154 134 107
103
BbeI G_GCGC'C
Cuts at: 2428 2676 2428
Size: 248 4748
BbsI GAAGACnn'nnnn_
Cuts at: 829 1377 829
Size: 548 4448
BbvCI CC'TCA_GC
Cuts at: 427 427
Size: 4996
BciVI GTATCCnnnnn_n'
Cuts at: 161 1179 2892 3655 4996 161
Size: 1018 1713 763 1341 161
Fragments arranged by size:
1713 1341 1018 763 161
BclI T'GATC_A
Cuts at: 2514 2514
Size: 4996
BglI GCCn_nnn'nGGC
Cuts at: 811 1728 811
Size: 917 4079
BglII A'GATC_T
Cuts at: 1631 2509 4548 1631
Size: 878 2039 2079
Fragments arranged by size:
2079 2039 878
BlpI GC'TnA_GC
Cuts at: 950 2106 950
Size: 1156 3840
Bme1580I G_kGCm'C
Cuts at: 227 464 634 1082 1232 2619 2712 3770 227
Size: 237 170 448 150 1387 93 1058 1453
Fragments arranged by size:
1453 1387 1058 448 237 170 150 93
BmrI ACTGGGnnnn_n'
Cuts at: 181 2206 2287 2622 4768 181
Size: 2025 81 335 2146 409
Fragments arranged by size:
2146 2025 409 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 257 3361 257
Size: 3104 1892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 1367 1519 1527 1754 1898 656
Size: 711 152 8 227 144 3754
Fragments arranged by size:
3754 711 227 152 144 8
Bpu10I CC'TnA_GC
Cuts at: 427 818 1295 427
Size: 391 477 4128
Fragments arranged by size:
4128 477 391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 1144 2170 3049 3543 3841 1132
Size: 12 1026 879 494 298 2287
Fragments arranged by size:
2287 1026 879 494 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 4700 4700
Size: 4996
BsaAI yAC'GTr
Cuts at: 768 1986 2347 768
Size: 1218 361 3417
Fragments arranged by size:
3417 1218 361
BsaBI GATnn'nnATC
Cuts at: 6 2508 4547 6
Size: 2502 2039 455
Fragments arranged by size:
2502 2039 455
BsaHI Gr'CG_yC
Cuts at: 2425 2673 3377 4873 2425
Size: 248 704 1496 2548
Fragments arranged by size:
2548 1496 704 248
BsaWI w'CCGG_w
Cuts at: 164 391 2183 2704 3384 3658 3805 4216 164
Size: 227 1792 521 680 274 147 411 944
Fragments arranged by size:
1792 944 680 521 411 274 227 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 1204 1234 1418 1448 655
Size: 30 519 30 184 30 4203
Fragments arranged by size:
4203 519 184 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 492 604 676 706 1072 1093 1442 1445 1901
Size: 112 72 30 366 21 349 3 456
Cuts at: 1901 492
Size: 3587
Fragments arranged by size:
3587 456 366 349 112 72 30 21
3
BseYI C'CCAG_C
Cuts at: 415 1074 1177 1257 2419 3177 3756 4252 415
Size: 659 103 80 1162 758 579 496 1159
Fragments arranged by size:
1162 1159 758 659 579 496 103 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1138
Size: 4996
BsiEI CG_ry'CG
Cuts at: 104 2536 2582 3792 104
Size: 2432 46 1210 1308
Fragments arranged by size:
2432 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1064 2008 2786 2976 3770 1064
Size: 944 778 190 794 2290
Fragments arranged by size:
2290 944 794 778 190
BsiWI C'GTAC_G
Cuts at: 277 277
Size: 4996
BsmBI CGTCTCn'nnnn_
Cuts at: 1683 1683
Size: 4996
Bsp1286I G_dGCh'C
Cuts at: 227 330 464 634 1064 1082 1232 1747 1854
Size: 103 134 170 430 18 150 515 107
Cuts at: 1854 2008 2619 2712 2786 2976 3038 3770 227
Size: 154 611 93 74 190 62 732 1453
Fragments arranged by size:
1453 732 611 515 430 190 170 154
150 134 107 103 93 74 62 18
BspEI T'CCGG_A
Cuts at: 4216 4216
Size: 4996
BspHI T'CATG_A
Cuts at: 1127 4172 1127
Size: 3045 1951
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2044 2258 2560 2941 2044
Size: 214 302 381 4099
Fragments arranged by size:
4099 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3286 3340 4826 4991 50
Size: 3236 54 1486 165 55
Fragments arranged by size:
3236 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 350 1663 2906 350
Size: 1313 1243 2440
Fragments arranged by size:
2440 1313 1243
BsrFI r'CCGG_y
Cuts at: 974 2992 3173 3384 974
Size: 2018 181 211 2586
Fragments arranged by size:
2586 2018 211 181
BssHII G'CGCG_C
Cuts at: 195 195
Size: 4996
BssSI C'ACGA_G
Cuts at: 967 3265 3625 967
Size: 2298 360 2338
Fragments arranged by size:
2338 2298 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1086 1594 2371 1086
Size: 508 777 3711
Fragments arranged by size:
3711 777 508
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4996
BstEII G'GTnAC_C
Cuts at: 1329 1516 1329
Size: 187 4809
BstXI CCAn_nnnn'nTGG
Cuts at: 624 1713 624
Size: 1089 3907
BstYI r'GATC_y
Cuts at: 1 933 1562 1631 2019 2486 2509 2844 3090
Size: 932 629 69 388 467 23 335 246
Cuts at: 3090 4104 4190 4202 4548 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 932 629 467 449 388 346 335
246 86 69 23 12
BstZ17I GTA'TAC
Cuts at: 1464 1464
Size: 4996
Bsu36I CC'TnA_GG
Cuts at: 475 1340 475
Size: 865 4131
BtgI C'CryG_G
Cuts at: 809 809
Size: 4996
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2944 3125 3288 2944
Size: 181 163 4652
Fragments arranged by size:
4652 181 163
BtsI GCAGTG_nn'
Cuts at: 366 2237 366
Size: 1871 3125
DraI TTT'AAA
Cuts at: 1993 1993
Size: 4996
DrdI GACnn_nn'nnGTC
Cuts at: 2700 3560 2700
Size: 860 4136
EaeI y'GGCC_r
Cuts at: 153 803 1720 1844 2579 2753 3144 3171 4518
Size: 650 917 124 735 174 391 27 1347
Cuts at: 4518 153
Size: 631
Fragments arranged by size:
1347 917 735 650 631 391 174 124
27
EagI C'GGCC_G
Cuts at: 2579 2579
Size: 4996
EarI CTCTTCn'nnn_
Cuts at: 653 3017 3227 653
Size: 2364 210 2422
Fragments arranged by size:
2422 2364 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3202 3514 3660 4806 3202
Size: 312 146 1146 3392
Fragments arranged by size:
3392 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1062 2006 1062
Size: 944 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 677 1367 1395 1519 1527 1754 1898 2819
Size: 21 690 28 124 8 227 144 921
Cuts at: 2819 3251 4000 4268 656
Size: 432 749 268 1384
Fragments arranged by size:
1384 921 749 690 432 268 227 144
124 28 21 8
EcoO109I rG'GnC_Cy
Cuts at: 223 224 315 451 460 630 631 825 1729
Size: 1 91 136 9 170 1 194 904
Cuts at: 1729 1875 2133 223
Size: 146 258 3086
Fragments arranged by size:
3086 904 258 194 170 146 136 91
9 1 1
EcoRI G'AATT_C
Cuts at: 1754 1998 1754
Size: 244 4752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1972 3013 1972
Size: 1041 3955
FspI TGC'GCA
Cuts at: 2775 2775
Size: 4996
HaeII r_GCGC'y
Cuts at: 975 1239 1572 2428 2676 3700 4679 975
Size: 264 333 856 248 1024 979 1292
Fragments arranged by size:
1292 1024 979 856 333 264 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 655 687 2836 2866 2868 2898 253
Size: 32 370 32 2149 30 2 30 2351
Fragments arranged by size:
2351 2149 370 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2033 2033
Size: 4996
KasI G'GCGC_C
Cuts at: 2424 2672 2424
Size: 248 4748
KpnI G_GTAC'C
Cuts at: 260 2014 260
Size: 1754 3242
MluI A'CGCG_T
Cuts at: 4429 4429
Size: 4996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 467 545 605 1948 3667 3851 467
Size: 78 60 1343 1719 184 1612
Fragments arranged by size:
1719 1612 1343 184 78 60
MscI TGG'CCA
Cuts at: 805 1846 2755 805
Size: 1041 909 3046
Fragments arranged by size:
3046 1041 909
MslI CAynn'nnrTG
Cuts at: 986 1480 2983 3110 986
Size: 494 1503 127 2872
Fragments arranged by size:
2872 1503 494 127
MspA1I CmG'CkG
Cuts at: 1074 1092 1122 1140 2105 2419 2779 3794 4039
Size: 18 30 18 965 314 360 1015 245
Cuts at: 4039 1074
Size: 2031
Fragments arranged by size:
2031 1015 965 360 314 245 30 18
18
NaeI GCC'GGC
Cuts at: 976 3175 976
Size: 2199 2797
NarI GG'CG_CC
Cuts at: 2425 2673 2425
Size: 248 4748
NcoI C'CATG_G
Cuts at: 809 809
Size: 4996
NdeI CA'TA_TG
Cuts at: 549 983 549
Size: 434 4562
NgoMIV G'CCGG_C
Cuts at: 974 3173 974
Size: 2199 2797
NruI TCG'CGA
Cuts at: 305 3357 305
Size: 3052 1944
NspI r_CATG'y
Cuts at: 192 203 1027 1624 2047 2547 3456 192
Size: 11 824 597 423 500 909 1732
Fragments arranged by size:
1732 909 824 597 500 423 11
PasI CC'CwG_GG
Cuts at: 211 457 1422 1776 211
Size: 246 965 354 3431
Fragments arranged by size:
3431 965 354 246
PciI A'CATG_T
Cuts at: 3452 3452
Size: 4996
PflMI CCAn_nnn'nTGG
Cuts at: 1558 1558
Size: 4996
PmeI GTTT'AAAC
Cuts at: 1993 1993
Size: 4996
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4135 4167 4135
Size: 32 4964
PpuMI rG'GwC_Cy
Cuts at: 315 825 315
Size: 510 4486
PshAI GACnn'nnGTC
Cuts at: 533 533
Size: 4996
PspOMI G'GGCC_C
Cuts at: 223 460 630 223
Size: 237 170 4589
Fragments arranged by size:
4589 237 170
PstI C_TGCA'G
Cuts at: 335 1091 1194 1583 1590 2041 2726 335
Size: 756 103 389 7 451 685 2605
Fragments arranged by size:
2605 756 685 451 389 103 7
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4996
PvuII CAG'CTG
Cuts at: 1074 1092 1140 2419 2779 1074
Size: 18 48 1279 360 3291
Fragments arranged by size:
3291 1279 360 48 18
RsrII CG'GwC_CG
Cuts at: 138 3189 138
Size: 3051 1945
SacI G_AGCT'C
Cuts at: 1064 2008 1064
Size: 944 4052
SalI G'TCGA_C
Cuts at: 2031 2031
Size: 4996
SanDI GG'GwC_CC
Cuts at: 825 825
Size: 4996
SapI GCTCTTCn'nnn_
Cuts at: 3017 3227 3017
Size: 210 4786
SbfI CC_TGCA'GG
Cuts at: 1583 2041 1583
Size: 458 4538
ScaI AGT'ACT
Cuts at: 4513 4513
Size: 4996
SfcI C'TryA_G
Cuts at: 33 331 1087 1190 1579 1586 2037 2722 3717
Size: 298 756 103 389 7 451 685 995
Cuts at: 3717 3908 33
Size: 191 1121
Fragments arranged by size:
1121 995 756 685 451 389 298 191
103 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 1728 1728
Size: 4996
SfoI GGC'GCC
Cuts at: 2426 2674 2426
Size: 248 4748
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4996
SmaI CCC'GGG
Cuts at: 2016 2016
Size: 4996
SmlI C'TyrA_G
Cuts at: 1147 1159 2149 3064 3558 3820 1147
Size: 12 990 915 494 262 2323
Fragments arranged by size:
2323 990 915 494 262 12
SnaBI TAC'GTA
Cuts at: 1986 1986
Size: 4996
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4996
SphI G_CATG'C
Cuts at: 192 203 2047 2547 192
Size: 11 1844 500 2641
Fragments arranged by size:
2641 1844 500 11
StuI AGG'CCT
Cuts at: 1609 1609
Size: 4996
StyI C'CwwG_G
Cuts at: 229 809 1512 1878 2128 2482 229
Size: 580 703 366 250 354 2743
Fragments arranged by size:
2743 703 580 366 354 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1689 1809 1845 2577 2710 3169 3380 1689
Size: 120 36 732 133 459 211 3305
Fragments arranged by size:
3305 732 459 211 133 120 36
TatI w'GTAC_w
Cuts at: 995 4511 995
Size: 3516 1480
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1204 1236 1418 1450 4513 4545 1204
Size: 32 182 32 3063 32 1655
Fragments arranged by size:
3063 1655 182 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 669 2791 669
Size: 2122 2874
XbaI T'CTAG_A
Cuts at: 2025 2025
Size: 4996
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 554 1839 2489 554
Size: 1285 650 3061
Fragments arranged by size:
3061 1285 650
XmaI C'CCGG_G
Cuts at: 2014 2014
Size: 4996
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsmI BsrGI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NsiI PacI PfoI PmlI PsiI
PspXI PsrI SacII SexAI SgrAI SrfI SspI SwaI
XhoI XmnI ZraI