Restriction map for
pF1KSDB0012
(Circular) (Six-base) MAPSORT of: pF1KSDB0012.seq Check: 7116 from: 1 to: 4570
pF1KSDB0012 4570 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 148 148
Size: 4570
AccI GT'mk_AC
Cuts at: 1606 1606
Size: 4570
Acc65I G'GTAC_C
Cuts at: 1584 1584
Size: 4570
AclI AA'CG_TT
Cuts at: 4410 4410
Size: 4570
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1378 1516 2393 2825 3574 3842 818
Size: 560 138 877 432 749 268 1546
Fragments arranged by size:
1546 877 749 560 432 268 138
AflIII A'CryG_T
Cuts at: 1920 3026 4003 1920
Size: 1106 977 2487
Fragments arranged by size:
2487 1106 977
AgeI A'CCGG_T
Cuts at: 2958 2958
Size: 4570
AlwNI CAG_nnn'CTG
Cuts at: 577 1084 1873 3442 577
Size: 507 789 1569 1705
Fragments arranged by size:
1705 1569 789 507
ApaI G_GGCC'C
Cuts at: 189 189
Size: 4570
ApaLI G'TGCA_C
Cuts at: 3340 3340
Size: 4570
ApoI r'AATT_y
Cuts at: 1150 1572 3923 4179 1150
Size: 422 2351 256 1541
Fragments arranged by size:
2351 1541 422 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4570
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4570
AvaI C'yCGr_G
Cuts at: 642 1588 642
Size: 946 3624
BamHI G'GATC_C
Cuts at: 1593 1593
Size: 4570
BanI G'GyrC_C
Cuts at: 206 564 865 1288 1584 1998 2246 2281 206
Size: 358 301 423 296 414 248 35 2495
Fragments arranged by size:
2495 423 414 358 301 296 248 35
BanII G_rGCy'C
Cuts at: 189 319 1410 1582 2612 189
Size: 130 1091 172 1030 2147
Fragments arranged by size:
2147 1091 1030 172 130
BbeI G_GCGC'C
Cuts at: 568 2002 2250 568
Size: 1434 248 2888
Fragments arranged by size:
2888 1434 248
BbsI GAAGACnn'nnnn_
Cuts at: 1504 1504
Size: 4570
BbvCI CC'TCA_GC
Cuts at: 845 1220 845
Size: 375 4195
BciVI GTATCCnnnnn_n'
Cuts at: 786 2466 3229 4570 786
Size: 1680 763 1341 786
Fragments arranged by size:
1680 1341 786 763
BclI T'GATC_A
Cuts at: 2088 2088
Size: 4570
BglI GCCn_nnn'nGGC
Cuts at: 241 424 535 241
Size: 183 111 4276
Fragments arranged by size:
4276 183 111
BglII A'GATC_T
Cuts at: 2083 4122 2083
Size: 2039 2531
BlpI GC'TnA_GC
Cuts at: 1680 1680
Size: 4570
Bme1580I G_kGCm'C
Cuts at: 189 2193 2286 3344 189
Size: 2004 93 1058 1415
Fragments arranged by size:
2004 1415 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 241 487 1780 1861 2196 4342 241
Size: 246 1293 81 335 2146 469
Fragments arranged by size:
2146 1293 469 335 246 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1248 2935 1248
Size: 1687 2883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 649 427
Size: 222 4348
Bpu10I CC'TnA_GC
Cuts at: 845 1220 845
Size: 375 4195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1744 2623 3117 3415 367
Size: 1377 879 494 298 1522
Fragments arranged by size:
1522 1377 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 632 4274 632
Size: 3642 928
BsaAI yAC'GTr
Cuts at: 1560 1921 1560
Size: 361 4209
BsaBI GATnn'nnATC
Cuts at: 6 2082 4121 6
Size: 2076 2039 455
Fragments arranged by size:
2076 2039 455
BsaHI Gr'CG_yC
Cuts at: 393 565 1999 2247 2951 4447 393
Size: 172 1434 248 704 1496 516
Fragments arranged by size:
1496 1434 704 516 248 172
BsaWI w'CCGG_w
Cuts at: 1757 2278 2958 3232 3379 3790 1757
Size: 521 680 274 147 411 2537
Fragments arranged by size:
2537 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 647 677 1004 1034 647
Size: 30 327 30 4183
Fragments arranged by size:
4183 327 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 319 724 736 739 847 1237 316
Size: 3 405 12 3 108 390 3649
Fragments arranged by size:
3649 405 390 108 12 3 3
BseYI C'CCAG_C
Cuts at: 539 570 1993 2751 3330 3826 539
Size: 31 1423 758 579 496 1283
Fragments arranged by size:
1423 1283 758 579 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 442 315
Size: 127 4443
BsiEI CG_ry'CG
Cuts at: 104 636 2110 2156 3366 104
Size: 532 1474 46 1210 1308
Fragments arranged by size:
1474 1308 1210 532 46
BsiHKAI G_wGCw'C
Cuts at: 1582 2360 2550 3344 1582
Size: 778 190 794 2808
Fragments arranged by size:
2808 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 740 740
Size: 4570
Bsp1286I G_dGCh'C
Cuts at: 189 319 1410 1582 2193 2286 2360 2550 2612
Size: 130 1091 172 611 93 74 190 62
Cuts at: 2612 3344 189
Size: 732 1415
Fragments arranged by size:
1415 1091 732 611 190 172 130 93
74 62
BspEI T'CCGG_A
Cuts at: 3790 3790
Size: 4570
BspHI T'CATG_A
Cuts at: 3746 3746
Size: 4570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 148 227 1618 1832 2134 2515 148
Size: 79 1391 214 302 381 2203
Fragments arranged by size:
2203 1391 381 302 214 79
BsrBI CCG'CTC
Cuts at: 50 1435 2860 2914 4400 4565 50
Size: 1385 1425 54 1486 165 55
Fragments arranged by size:
1486 1425 1385 165 55 54
BsrDI GCAATG_nn'
Cuts at: 809 2480 809
Size: 1671 2899
BsrFI r'CCGG_y
Cuts at: 2566 2747 2958 2566
Size: 181 211 4178
Fragments arranged by size:
4178 211 181
BsrGI T'GTAC_A
Cuts at: 1086 1086
Size: 4570
BssSI C'ACGA_G
Cuts at: 2839 3199 2839
Size: 360 4210
BstAPI GCAn_nnn'nTGC
Cuts at: 149 338 1037 1945 149
Size: 189 699 908 2774
Fragments arranged by size:
2774 908 699 189
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4570
BstYI r'GATC_y
Cuts at: 1 1593 2060 2083 2418 2664 3678 3764 3776
Size: 1592 467 23 335 246 1014 86 12
Cuts at: 3776 4122 1
Size: 346 449
Fragments arranged by size:
1592 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 605 621 1265 1379 125
Size: 480 16 644 114 3316
Fragments arranged by size:
3316 644 480 114 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1151 1288 2518 2699 2862 1151
Size: 137 1230 181 163 2859
Fragments arranged by size:
2859 1230 181 163 137
BtsI GCAGTG_nn'
Cuts at: 434 1811 434
Size: 1377 3193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1334 1369 1334
Size: 35 4535
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4570
DrdI GACnn_nn'nnGTC
Cuts at: 2274 3134 2274
Size: 860 3710
EaeI y'GGCC_r
Cuts at: 233 320 515 602 618 2153 2327 2718 2745
Size: 87 195 87 16 1535 174 391 27
Cuts at: 2745 4092 233
Size: 1347 711
Fragments arranged by size:
1535 1347 711 391 195 174 87 87
27 16
EagI C'GGCC_G
Cuts at: 2153 2153
Size: 4570
EarI CTCTTCn'nnn_
Cuts at: 725 833 1129 2591 2801 725
Size: 108 296 1462 210 2494
Fragments arranged by size:
2494 1462 296 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 240 2776 3088 3234 4380 240
Size: 2536 312 146 1146 430
Fragments arranged by size:
2536 1146 430 312 146
EcoICRI GAG'CTC
Cuts at: 1580 1580
Size: 4570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 649 818 1378 1516 2393 2825 3574 3842
Size: 222 169 560 138 877 432 749 268
Cuts at: 3842 427
Size: 1155
Fragments arranged by size:
1155 877 749 560 432 268 222 169
138
EcoO109I rG'GnC_Cy
Cuts at: 626 735 1206 1707 626
Size: 109 471 501 3489
Fragments arranged by size:
3489 501 471 109
EcoRI G'AATT_C
Cuts at: 1572 1572
Size: 4570
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1056 2587 1056
Size: 1531 3039
FspI TGC'GCA
Cuts at: 2349 2349
Size: 4570
HaeII r_GCGC'y
Cuts at: 568 577 2002 2250 3274 4253 568
Size: 9 1425 248 1024 979 885
Fragments arranged by size:
1425 1024 979 885 248 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1167 1199 2410 2440 2442 2472 1167
Size: 32 1211 30 2 30 3265
Fragments arranged by size:
3265 1211 32 30 30 2
HincII GTy'rAC
Cuts at: 1607 1607
Size: 4570
KasI G'GCGC_C
Cuts at: 564 1998 2246 564
Size: 1434 248 2888
Fragments arranged by size:
2888 1434 248
KpnI G_GTAC'C
Cuts at: 1588 1588
Size: 4570
MluI A'CGCG_T
Cuts at: 4003 4003
Size: 4570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3241 3425 3241
Size: 184 4386
MscI TGG'CCA
Cuts at: 235 2329 235
Size: 2094 2476
MslI CAynn'nnrTG
Cuts at: 1100 2557 2684 1100
Size: 1457 127 2986
Fragments arranged by size:
2986 1457 127
MspA1I CmG'CkG
Cuts at: 183 288 1679 1993 2353 3368 3613 183
Size: 105 1391 314 360 1015 245 1140
Fragments arranged by size:
1391 1140 1015 360 314 245 105
NaeI GCC'GGC
Cuts at: 2749 2749
Size: 4570
NarI GG'CG_CC
Cuts at: 565 1999 2247 565
Size: 1434 248 2888
Fragments arranged by size:
2888 1434 248
NcoI C'CATG_G
Cuts at: 125 1265 125
Size: 1140 3430
NdeI CA'TA_TG
Cuts at: 1097 1097
Size: 4570
NgoMIV G'CCGG_C
Cuts at: 2747 2747
Size: 4570
NruI TCG'CGA
Cuts at: 2931 2931
Size: 4570
NspI r_CATG'y
Cuts at: 180 1461 1621 2121 3030 180
Size: 1281 160 500 909 1720
Fragments arranged by size:
1720 1281 909 500 160
PasI CC'CwG_GG
Cuts at: 1410 1410
Size: 4570
PciI A'CATG_T
Cuts at: 3026 3026
Size: 4570
PflMI CCAn_nnn'nTGG
Cuts at: 862 862
Size: 4570
PfoI T'CCnGG_A
Cuts at: 266 266
Size: 4570
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3709 3741 3709
Size: 32 4538
PpuMI rG'GwC_Cy
Cuts at: 626 735 1206 626
Size: 109 471 3990
Fragments arranged by size:
3990 471 109
PspOMI G'GGCC_C
Cuts at: 185 185
Size: 4570
PstI C_TGCA'G
Cuts at: 1615 2300 1615
Size: 685 3885
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4570
PvuII CAG'CTG
Cuts at: 1993 2353 1993
Size: 360 4210
RsrII CG'GwC_CG
Cuts at: 2763 2763
Size: 4570
SacI G_AGCT'C
Cuts at: 1582 1582
Size: 4570
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4570
SanDI GG'GwC_CC
Cuts at: 626 626
Size: 4570
SapI GCTCTTCn'nnn_
Cuts at: 1129 2591 2801 1129
Size: 1462 210 2898
Fragments arranged by size:
2898 1462 210
SbfI CC_TGCA'GG
Cuts at: 1615 1615
Size: 4570
ScaI AGT'ACT
Cuts at: 1391 4087 1391
Size: 2696 1874
SfcI C'TryA_G
Cuts at: 33 1611 2296 3291 3482 33
Size: 1578 685 995 191 1121
Fragments arranged by size:
1578 1121 995 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 241 535 241
Size: 294 4276
SfoI GGC'GCC
Cuts at: 566 2000 2248 566
Size: 1434 248 2888
Fragments arranged by size:
2888 1434 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4570
SmaI CCC'GGG
Cuts at: 1590 1590
Size: 4570
SmlI C'TyrA_G
Cuts at: 382 1723 2638 3132 3394 382
Size: 1341 915 494 262 1558
Fragments arranged by size:
1558 1341 915 494 262
SnaBI TAC'GTA
Cuts at: 1560 1560
Size: 4570
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4570
SphI G_CATG'C
Cuts at: 1621 2121 1621
Size: 500 4070
StyI C'CwwG_G
Cuts at: 125 861 1265 1702 2056 125
Size: 736 404 437 354 2639
Fragments arranged by size:
2639 736 437 404 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 650 2151 2284 2743 2954 650
Size: 1501 133 459 211 2266
Fragments arranged by size:
2266 1501 459 211 133
TatI w'GTAC_w
Cuts at: 1086 1389 1400 4085 1086
Size: 303 11 2685 1571
Fragments arranged by size:
2685 1571 303 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 370 402 467 499 647 679 4087 4119 370
Size: 32 65 32 148 32 3408 32 821
Fragments arranged by size:
3408 821 148 65 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2365 2365
Size: 4570
XbaI T'CTAG_A
Cuts at: 1599 1599
Size: 4570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 597 2063 597
Size: 1466 3104
XmaI C'CCGG_G
Cuts at: 1588 1588
Size: 4570
XmnI GAAnn'nnTTC
Cuts at: 1066 1274 1066
Size: 208 4362
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SgrAI SrfI SspI StuI SwaI XhoI ZraI