Restriction map for
pF1KSDB0014
(Circular) (Six-base) MAPSORT of: pF1KSDB0014.seq Check: 3431 from: 1 to: 9017
pF1KSDB0014 9017 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2631 2933 3951 4113 4713 5664 2631
Size: 302 1018 162 600 951 5984
Fragments arranged by size:
5984 1018 951 600 302 162
AatII G_ACGT'C
Cuts at: 2471 2471
Size: 9017
AccI GT'mk_AC
Cuts at: 6055 6055
Size: 9017
Acc65I G'GTAC_C
Cuts at: 6033 6033
Size: 9017
AclI AA'CG_TT
Cuts at: 8857 8857
Size: 9017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 1009 1150 1831 2476 2605 3661 5002 5170
Size: 470 141 681 645 129 1056 1341 168
Cuts at: 5170 5401 5677 5761 6842 7274 8021 8289 539
Size: 231 276 84 1081 432 747 268 1267
Fragments arranged by size:
1341 1267 1081 1056 747 681 645 470
432 276 268 231 168 141 129 84
AfeI AGC'GCT
Cuts at: 2914 4939 2914
Size: 2025 6992
AflII C'TTAA_G
Cuts at: 5494 5494
Size: 9017
AflIII A'CryG_T
Cuts at: 1044 1076 6369 7473 8450 1044
Size: 32 5293 1104 977 1611
Fragments arranged by size:
5293 1611 1104 977 32
AgeI A'CCGG_T
Cuts at: 7405 7405
Size: 9017
AhdI GACnn_n'nnGTC
Cuts at: 1212 3486 4008 1212
Size: 2274 522 6221
Fragments arranged by size:
6221 2274 522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2729 5650 2729
Size: 2921 6096
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 929 961 1708 1740 929
Size: 32 747 32 8206
Fragments arranged by size:
8206 747 32 32
AlwNI CAG_nnn'CTG
Cuts at: 696 759 2925 3009 3261 3789 3894 4071 4098
Size: 63 2166 84 252 528 105 177 27
Cuts at: 4098 4389 4473 5472 6322 7889 696
Size: 291 84 999 850 1567 1824
Fragments arranged by size:
2166 1824 1567 999 850 528 291 252
177 105 84 84 63 27
ApaI G_GGCC'C
Cuts at: 2838 4578 4713 2838
Size: 1740 135 7142
Fragments arranged by size:
7142 1740 135
ApaLI G'TGCA_C
Cuts at: 639 7787 639
Size: 7148 1869
ApoI r'AATT_y
Cuts at: 835 4140 6021 8370 8626 835
Size: 3305 1881 2349 256 1226
Fragments arranged by size:
3305 2349 1881 1226 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9017
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9017
AvaI C'yCGr_G
Cuts at: 1057 1689 2023 2218 3769 4538 4571 5560 6037
Size: 632 334 195 1551 769 33 989 477
Cuts at: 6037 1057
Size: 4037
Fragments arranged by size:
4037 1551 989 769 632 477 334 195
33
BamHI G'GATC_C
Cuts at: 1472 1893 6042 1472
Size: 421 4149 4447
Fragments arranged by size:
4447 4149 421
BanI G'GyrC_C
Cuts at: 225 1456 1777 4259 5938 6033 6447 6695 6730
Size: 1231 321 2482 1679 95 414 248 35
Cuts at: 6730 225
Size: 2512
Fragments arranged by size:
2512 2482 1679 1231 414 321 248 95
35
BanII G_rGCy'C
Cuts at: 392 452 2372 2831 2838 3326 3347 3380 3608
Size: 60 1920 459 7 488 21 33 228
Cuts at: 3608 4578 4660 4713 5280 6031 7061 392
Size: 970 82 53 567 751 1030 2348
Fragments arranged by size:
2348 1920 1030 970 751 567 488 459
228 82 60 53 33 21 7
BbeI G_GCGC'C
Cuts at: 1460 5942 6451 6699 1460
Size: 4482 509 248 3778
Fragments arranged by size:
4482 3778 509 248
BbsI GAAGACnn'nnnn_
Cuts at: 997 1176 2256 2400 3125 3274 3485 3652 4369
Size: 179 1080 144 725 149 211 167 717
Cuts at: 4369 4492 4744 997
Size: 123 252 5270
Fragments arranged by size:
5270 1080 725 717 252 211 179 167
149 144 123
BbvCI CC'TCA_GC
Cuts at: 2107 2822 3304 4274 4526 5987 2107
Size: 715 482 970 252 1461 5137
Fragments arranged by size:
5137 1461 970 715 482 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 187 221 2794 2828 187
Size: 34 2573 34 6376
Fragments arranged by size:
6376 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6915 7676 9017 6915
Size: 761 1341 6915
Fragments arranged by size:
6915 1341 761
BclI T'GATC_A
Cuts at: 3209 5366 6537 3209
Size: 2157 1171 5689
Fragments arranged by size:
5689 2157 1171
BglI GCCn_nnn'nGGC
Cuts at: 202 1369 1691 2243 3391 4222 4822 5563 5725
Size: 1167 322 552 1148 831 600 741 162
Cuts at: 5725 5788 202
Size: 63 3431
Fragments arranged by size:
3431 1167 1148 831 741 600 552 322
162 63
BglII A'GATC_T
Cuts at: 1496 2870 3461 6532 8569 1496
Size: 1374 591 3071 2037 1944
Fragments arranged by size:
3071 2037 1944 1374 591
BlpI GC'TnA_GC
Cuts at: 4099 6129 4099
Size: 2030 6987
Bme1580I G_kGCm'C
Cuts at: 643 1780 2838 4578 4713 5524 5918 6642 6735
Size: 1137 1058 1740 135 811 394 724 93
Cuts at: 6735 7791 643
Size: 1056 1869
Fragments arranged by size:
1869 1740 1137 1058 1056 811 724 394
135 93
BmgBI CAC'GTC
Cuts at: 1047 1047
Size: 9017
BmrI ACTGGGnnnn_n'
Cuts at: 210 4821 6229 6310 6645 8789 210
Size: 4611 1408 81 335 2144 438
Fragments arranged by size:
4611 2144 1408 438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1309 3309 7384 1309
Size: 2000 4075 2942
Fragments arranged by size:
4075 2942 2000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 766 799 998 1606 2386 2458 2545 2776
Size: 92 33 199 608 780 72 87 231
Cuts at: 2776 3001 3037 3073 3094 3283 3304 3310 3364
Size: 225 36 36 21 189 21 6 54
Cuts at: 3364 3457 3505 3673 3760 3811 4063 4180 4210
Size: 93 48 168 87 51 252 117 30
Cuts at: 4210 4279 4327 4378 4462 4600 4630 4714 4777
Size: 69 48 51 84 138 30 84 63
Cuts at: 4777 4939 4990 5011 5326 5347 5359 5449 5602
Size: 162 51 21 315 21 12 90 153
Cuts at: 5602 5782 5845 674
Size: 180 63 3846
Fragments arranged by size:
3846 780 608 315 252 231 225 199
189 180 168 162 153 138 117 93
92 90 87 87 84 84 72 69
63 63 54 51 51 51 48 48
36 36 33 30 30 21 21 21
21 12 6
Bpu10I CC'TnA_GC
Cuts at: 2107 2822 3304 3607 4105 4274 4315 4526 5887
Size: 715 482 303 498 169 41 211 1361
Cuts at: 5887 5987 2107
Size: 100 5137
Fragments arranged by size:
5137 1361 715 498 482 303 211 169
100 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 412 2989 3172 3331 3418 4102 5734 6193
Size: 165 2577 183 159 87 684 1632 459
Cuts at: 6193 7072 7564 7862 247
Size: 879 492 298 1402
Fragments arranged by size:
2577 1632 1402 879 684 492 459 298
183 165 159 87
BsaI GGTCTCn'nnnn_
Cuts at: 1081 2005 2444 4543 5596 8721 1081
Size: 924 439 2099 1053 3125 1377
Fragments arranged by size:
3125 2099 1377 1053 924 439
BsaAI yAC'GTr
Cuts at: 395 705 6009 6370 395
Size: 310 5304 361 3042
Fragments arranged by size:
5304 3042 361 310
BsaBI GATnn'nnATC
Cuts at: 6 149 6531 8568 6
Size: 143 6382 2037 455
Fragments arranged by size:
6382 2037 455 143
BsaHI Gr'CG_yC
Cuts at: 709 1213 1457 2468 5301 5831 5939 6448 6696
Size: 504 244 1011 2833 530 108 509 248
Cuts at: 6696 7398 8894 709
Size: 702 1496 832
Fragments arranged by size:
2833 1496 1011 832 702 530 509 504
248 244 108
BsaWI w'CCGG_w
Cuts at: 6206 6727 7405 7679 7826 8237 6206
Size: 521 678 274 147 411 6986
Fragments arranged by size:
6986 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 805 835 1708 1738 3595 3625 3908 3938 5530
Size: 30 873 30 1857 30 283 30 1592
Cuts at: 5530 5560 805
Size: 30 4262
Fragments arranged by size:
4262 1857 1592 873 283 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 283 810 1141 1609 1729 2455 2458 2671 2674
Size: 527 331 468 120 726 3 213 3
Cuts at: 2674 2692 2695 2839 2914 2917 2920 2923 2986
Size: 18 3 144 75 3 3 3 63
Cuts at: 2986 2998 3001 3004 3070 3073 3172 3175 3256
Size: 12 3 3 66 3 99 3 81
Cuts at: 3256 3385 3388 3670 3673 3736 4096 4177 4180
Size: 129 3 282 3 63 360 81 3
Cuts at: 4180 4183 4186 4291 4345 4495 4525 4744 4882
Size: 3 3 105 54 150 30 219 138
Cuts at: 4882 5125 5305 5344 5347 5356 5359 5599 5779
Size: 243 180 39 3 9 3 240 180
Cuts at: 5779 5791 283
Size: 12 3509
Fragments arranged by size:
3509 726 527 468 360 331 282 243
240 219 213 180 180 150 144 138
129 120 105 99 81 81 75 66
63 63 54 39 30 18 12 12
9 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 3596 4049 5222 5321 5783 6442 7200 7777 8273
Size: 453 1173 99 462 659 758 577 496
Cuts at: 8273 3596
Size: 4340
Fragments arranged by size:
4340 1173 758 659 577 496 462 453
99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 9017
BsiEI CG_ry'CG
Cuts at: 104 4521 5376 5846 6559 6605 7813 104
Size: 4417 855 470 713 46 1208 1308
Fragments arranged by size:
4417 1308 1208 855 713 470 46
BsiHKAI G_wGCw'C
Cuts at: 392 643 986 2351 2372 2831 3326 3347 3380
Size: 251 343 1365 21 459 495 21 33
Cuts at: 3380 3608 3860 4111 4660 6031 6809 6999 7791
Size: 228 252 251 549 1371 778 190 792
Cuts at: 7791 392
Size: 1618
Fragments arranged by size:
1618 1371 1365 792 778 549 495 459
343 252 251 251 228 190 33 21
21
BsiWI C'GTAC_G
Cuts at: 701 701
Size: 9017
BsmI GAATG_Cn'
Cuts at: 1694 5463 1694
Size: 3769 5248
BsmBI CGTCTCn'nnnn_
Cuts at: 2734 5833 2734
Size: 3099 5918
Bsp1286I G_dGCh'C
Cuts at: 392 452 643 986 1780 2351 2372 2831 2838
Size: 60 191 343 794 571 21 459 7
Cuts at: 2838 3326 3347 3380 3608 3860 4111 4578 4660
Size: 488 21 33 228 252 251 467 82
Cuts at: 4660 4713 5280 5524 5918 6031 6642 6735 6809
Size: 53 567 244 394 113 611 93 74
Cuts at: 6809 6999 7061 7791 392
Size: 190 62 730 1618
Fragments arranged by size:
1618 794 730 611 571 567 488 467
459 394 343 252 251 244 228 191
190 113 93 82 74 62 60 53
33 21 21 7
BspEI T'CCGG_A
Cuts at: 8237 8237
Size: 9017
BspHI T'CATG_A
Cuts at: 2351 8193 2351
Size: 5842 3175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 512 1655 2631 2933 3888 3951 4113 4406 4713
Size: 1143 976 302 955 63 162 293 307
Cuts at: 4713 4817 4850 5402 5664 5909 6006 6067 6281
Size: 104 33 552 262 245 97 61 214
Cuts at: 6281 6583 6964 512
Size: 302 381 2565
Fragments arranged by size:
2565 1143 976 955 552 381 307 302
302 293 262 245 214 162 104 97
63 61 33
BsrBI CCG'CTC
Cuts at: 50 750 1197 1395 3801 4614 4836 5232 5688
Size: 700 447 198 2406 813 222 396 456
Cuts at: 5688 7309 7363 8847 9012 50
Size: 1621 54 1484 165 55
Fragments arranged by size:
2406 1621 1484 813 700 456 447 396
222 198 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1481 3212 6929 1481
Size: 1731 3717 3569
Fragments arranged by size:
3717 3569 1731
BsrFI r'CCGG_y
Cuts at: 1486 1846 1999 2164 2419 2648 4088 5375 7015
Size: 360 153 165 255 229 1440 1287 1640
Cuts at: 7015 7196 7405 1486
Size: 181 209 3098
Fragments arranged by size:
3098 1640 1440 1287 360 255 229 209
181 165 153
BsrGI T'GTAC_A
Cuts at: 536 536
Size: 9017
BssSI C'ACGA_G
Cuts at: 556 911 2152 2185 3673 4327 4687 5105 7288
Size: 355 1241 33 1488 654 360 418 2183
Cuts at: 7288 7646 556
Size: 358 1927
Fragments arranged by size:
2183 1927 1488 1241 654 418 360 358
355 33
BstAPI GCAn_nnn'nTGC
Cuts at: 561 759 2187 3009 3894 6394 561
Size: 198 1428 822 885 2500 3184
Fragments arranged by size:
3184 2500 1428 885 822 198
BstBI TT'CG_AA
Cuts at: 110 7378 110
Size: 7268 1749
BstEII G'GTnAC_C
Cuts at: 3024 3948 3024
Size: 924 8093
BstXI CCAn_nnnn'nTGG
Cuts at: 216 2097 2259 216
Size: 1881 162 6974
Fragments arranged by size:
6974 1881 162
BstYI r'GATC_y
Cuts at: 1 1472 1496 1893 2291 2870 3440 3461 3683
Size: 1471 24 397 398 579 570 21 222
Cuts at: 3683 5114 6042 6509 6532 6867 7113 8125 8211
Size: 1431 928 467 23 335 246 1012 86
Cuts at: 8211 8223 8569 1
Size: 12 346 449
Fragments arranged by size:
1471 1431 1012 928 579 570 467 449
398 397 346 335 246 222 86 24
23 21 12
BtgI C'CryG_G
Cuts at: 125 1091 3716 4589 5198 125
Size: 966 2625 873 609 3944
Fragments arranged by size:
3944 2625 966 873 609
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1896 5301 6967 7148 7311 1896
Size: 3405 1666 181 163 3602
Fragments arranged by size:
3602 3405 1666 181 163
BtsI GCAGTG_nn'
Cuts at: 1918 2017 2608 4955 6260 1918
Size: 99 591 2347 1305 4675
Fragments arranged by size:
4675 2347 1305 591 99
DraI TTT'AAA
Cuts at: 6016 6016
Size: 9017
DrdI GACnn_nn'nnGTC
Cuts at: 1945 2748 6723 7581 1945
Size: 803 3975 858 3381
Fragments arranged by size:
3975 3381 858 803
EaeI y'GGCC_r
Cuts at: 1376 1997 2087 2394 2507 2669 3095 3359 3392
Size: 621 90 307 113 162 426 264 33
Cuts at: 3392 3812 3938 4223 4340 4952 5009 5843 6602
Size: 420 126 285 117 612 57 834 759
Cuts at: 6602 6776 7167 7194 8539 1376
Size: 174 391 27 1345 1854
Fragments arranged by size:
1854 1345 834 759 621 612 426 420
391 307 285 264 174 162 126 117
113 90 57 33 27
EagI C'GGCC_G
Cuts at: 5843 6602 5843
Size: 759 8258
EarI CTCTTCn'nnn_
Cuts at: 275 515 544 1118 1567 2620 2861 3149 3218
Size: 240 29 574 449 1053 241 288 69
Cuts at: 3218 3398 3536 4279 4745 5146 5768 5954 7040
Size: 180 138 743 466 401 622 186 1086
Cuts at: 7040 7250 275
Size: 210 2042
Fragments arranged by size:
2042 1086 1053 743 622 574 466 449
401 288 241 240 210 186 180 138
69 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 910 2947 3745 4492 4546 4618 7225 7535 7681
Size: 2037 798 747 54 72 2607 310 146
Cuts at: 7681 8827 910
Size: 1146 1100
Fragments arranged by size:
2607 2037 1146 1100 798 747 310 146
72 54
EcoICRI GAG'CTC
Cuts at: 390 2370 2829 3324 3345 3378 3606 4658 6029
Size: 1980 459 495 21 33 228 1052 1371
Cuts at: 6029 390
Size: 3378
Fragments arranged by size:
3378 1980 1371 1052 495 459 228 33
21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 674 766 799 998 1009 1150 1606 1831
Size: 135 92 33 199 11 141 456 225
Cuts at: 1831 2386 2458 2476 2545 2605 2776 3001 3037
Size: 555 72 18 69 60 171 225 36
Cuts at: 3037 3073 3094 3283 3304 3310 3364 3457 3505
Size: 36 21 189 21 6 54 93 48
Cuts at: 3505 3661 3673 3760 3811 4063 4180 4210 4279
Size: 156 12 87 51 252 117 30 69
Cuts at: 4279 4327 4378 4462 4600 4630 4714 4777 4939
Size: 48 51 84 138 30 84 63 162
Cuts at: 4939 4990 5002 5011 5170 5326 5347 5359 5401
Size: 51 12 9 159 156 21 12 42
Cuts at: 5401 5449 5602 5677 5761 5782 5845 6842 7274
Size: 48 153 75 84 21 63 997 432
Cuts at: 7274 8021 8289 539
Size: 747 268 1267
Fragments arranged by size:
1267 997 747 555 456 432 268 252
225 225 199 189 171 162 159 156
156 153 141 138 135 117 93 92
87 84 84 84 75 72 69 69
63 63 60 54 51 51 51 48
48 48 42 36 36 33 30 30
21 21 21 21 18 12 12 12
11 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 265 1588 1990 2440 2493 3655 3772 4477 5122
Size: 1323 402 450 53 1162 117 705 645
Cuts at: 5122 265
Size: 4160
Fragments arranged by size:
4160 1323 1162 705 645 450 402 117
53
EcoO109I rG'GnC_Cy
Cuts at: 1684 2027 2556 3531 4302 4311 4575 4647 4710
Size: 343 529 975 771 9 264 72 63
Cuts at: 4710 4839 4986 5898 6156 1684
Size: 129 147 912 258 4545
Fragments arranged by size:
4545 975 912 771 529 343 264 258
147 129 72 63 9
EcoRI G'AATT_C
Cuts at: 4140 6021 4140
Size: 1881 7136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 261 4161 7036 261
Size: 3900 2875 2242
Fragments arranged by size:
3900 2875 2242
FspI TGC'GCA
Cuts at: 1420 3928 6798 1420
Size: 2508 2870 3639
Fragments arranged by size:
3639 2870 2508
FspAI rTGC'GCAy
Cuts at: 3928 3928
Size: 9017
HaeII r_GCGC'y
Cuts at: 1460 2916 2999 3231 3556 4425 4941 5217 5616
Size: 1456 83 232 325 869 516 276 399
Cuts at: 5616 5942 6451 6699 7721 8700 1460
Size: 326 509 248 1022 979 1777
Fragments arranged by size:
1777 1456 1022 979 869 516 509 399
326 325 276 248 232 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 552 584 805 837 1198 1230 1488 1495 1520
Size: 32 221 32 361 32 258 7 25
Cuts at: 1520 1527 2276 2283 2308 2315 3151 3183 3439
Size: 7 749 7 25 7 836 32 256
Cuts at: 3439 3471 3472 3504 3595 3627 3994 4026 4542
Size: 32 1 32 91 32 367 32 516
Cuts at: 4542 4574 5713 5745 6859 6889 6891 6921 552
Size: 32 1139 32 1114 30 2 30 2648
Fragments arranged by size:
2648 1139 1114 836 749 516 367 361
258 256 221 91 32 32 32 32
32 32 32 32 32 32 30 30
25 25 7 7 7 7 2 1
HincII GTy'rAC
Cuts at: 4806 6056 4806
Size: 1250 7767
KasI G'GCGC_C
Cuts at: 1456 5938 6447 6695 1456
Size: 4482 509 248 3778
Fragments arranged by size:
4482 3778 509 248
KpnI G_GTAC'C
Cuts at: 6037 6037
Size: 9017
MluI A'CGCG_T
Cuts at: 8450 8450
Size: 9017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 257 855 986 1988 3548 4025 4778 5620 5978
Size: 598 131 1002 1560 477 753 842 358
Cuts at: 5978 7688 7872 257
Size: 1710 184 1402
Fragments arranged by size:
1710 1560 1402 1002 842 753 598 477
358 184 131
MscI TGG'CCA
Cuts at: 1378 2089 2396 2509 2671 3097 3361 3394 3814
Size: 711 307 113 162 426 264 33 420
Cuts at: 3814 6778 1378
Size: 2964 3617
Fragments arranged by size:
3617 2964 711 426 420 307 264 162
113 33
MslI CAynn'nnrTG
Cuts at: 575 1309 2734 4639 7006 7133 575
Size: 734 1425 1905 2367 127 2459
Fragments arranged by size:
2459 2367 1905 1425 734 127
MspA1I CmG'CkG
Cuts at: 186 756 1234 1406 1524 1809 2085 2135 2199
Size: 570 478 172 118 285 276 50 64
Cuts at: 2199 2565 2634 2709 2778 2979 3015 3357 3592
Size: 366 69 75 69 201 36 342 235
Cuts at: 3592 3738 3885 4053 4158 4323 4746 4884 5043
Size: 146 147 168 105 165 423 138 159
Cuts at: 5043 5200 5267 5325 5472 5580 5592 5760 5787
Size: 157 67 58 147 108 12 168 27
Cuts at: 5787 6128 6442 6802 7815 8060 186
Size: 341 314 360 1013 245 1143
Fragments arranged by size:
1143 1013 570 478 423 366 360 342
341 314 285 276 245 235 201 172
168 168 165 159 157 147 147 146
138 118 108 105 75 69 69 67
64 58 50 36 27 12
NaeI GCC'GGC
Cuts at: 1488 1848 2001 2166 2650 7198 1488
Size: 360 153 165 484 4548 3307
Fragments arranged by size:
4548 3307 484 360 165 153
NarI GG'CG_CC
Cuts at: 1457 5939 6448 6696 1457
Size: 4482 509 248 3778
Fragments arranged by size:
4482 3778 509 248
NcoI C'CATG_G
Cuts at: 125 1091 4589 125
Size: 966 3498 4553
Fragments arranged by size:
4553 3498 966
NgoMIV G'CCGG_C
Cuts at: 1486 1846 1999 2164 2648 7196 1486
Size: 360 153 165 484 4548 3307
Fragments arranged by size:
4548 3307 484 360 165 153
NspI r_CATG'y
Cuts at: 1080 2007 2052 5079 6070 6570 7477 1080
Size: 927 45 3027 991 500 907 2620
Fragments arranged by size:
3027 2620 991 927 907 500 45
PciI A'CATG_T
Cuts at: 1076 7473 1076
Size: 6397 2620
PflMI CCAn_nnn'nTGG
Cuts at: 696 1257 4473 4692 696
Size: 561 3216 219 5021
Fragments arranged by size:
5021 3216 561 219
PfoI T'CCnGG_A
Cuts at: 776 1082 1475 1583 2267 2960 3071 3443 3473
Size: 306 393 108 684 693 111 372 30
Cuts at: 3473 3502 3686 4148 776
Size: 29 184 462 5645
Fragments arranged by size:
5645 693 684 462 393 372 306 184
111 108 30 29
PmeI GTTT'AAAC
Cuts at: 6016 6016
Size: 9017
PmlI CAC'GTG
Cuts at: 395 395
Size: 9017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1384 1416 1708 1740 8156 8188 1384
Size: 32 292 32 6416 32 2213
Fragments arranged by size:
6416 2213 292 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2556 3531 4302 4647 4839 4986 5898 2556
Size: 975 771 345 192 147 912 5675
Fragments arranged by size:
5675 975 912 771 345 192 147
PspOMI G'GGCC_C
Cuts at: 2834 4574 4709 2834
Size: 1740 135 7142
Fragments arranged by size:
7142 1740 135
PspXI vC'TCGA_Gb
Cuts at: 5560 5560
Size: 9017
PstI C_TGCA'G
Cuts at: 764 1556 1652 2497 2642 2750 2783 2930 3014
Size: 792 96 845 145 108 33 147 84
Cuts at: 3014 3839 3890 3899 3962 4058 4079 4382 4772
Size: 825 51 9 63 96 21 303 390
Cuts at: 4772 4814 4847 5057 5399 5510 5675 5816 6064
Size: 42 33 210 342 111 165 141 248
Cuts at: 6064 6749 764
Size: 685 3032
Fragments arranged by size:
3032 845 825 792 685 390 342 303
248 210 165 147 145 141 111 108
96 96 84 63 51 42 33 33
21 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9017
PvuII CAG'CTG
Cuts at: 756 1234 1524 1809 2085 2199 2709 2778 2979
Size: 478 290 285 276 114 510 69 201
Cuts at: 2979 3015 3357 3592 3738 3885 4053 4158 4746
Size: 36 342 235 146 147 168 105 588
Cuts at: 4746 4884 5043 5325 5472 5580 5592 5760 6442
Size: 138 159 282 147 108 12 168 682
Cuts at: 6442 6802 756
Size: 360 2971
Fragments arranged by size:
2971 682 588 510 478 360 342 290
285 282 276 235 201 168 168 159
147 147 146 138 114 108 105 69
36 12
RsrII CG'GwC_CG
Cuts at: 5642 7212 5642
Size: 1570 7447
SacI G_AGCT'C
Cuts at: 392 2372 2831 3326 3347 3380 3608 4660 6031
Size: 1980 459 495 21 33 228 1052 1371
Cuts at: 6031 392
Size: 3378
Fragments arranged by size:
3378 1980 1371 1052 495 459 228 33
21
SacII CC_GC'GG
Cuts at: 5201 5201
Size: 9017
SalI G'TCGA_C
Cuts at: 6054 6054
Size: 9017
SapI GCTCTTCn'nnn_
Cuts at: 1567 2620 3218 3536 4745 5146 7040 7250 1567
Size: 1053 598 318 1209 401 1894 210 3334
Fragments arranged by size:
3334 1894 1209 1053 598 401 318 210
SbfI CC_TGCA'GG
Cuts at: 2930 4814 4847 6064 2930
Size: 1884 33 1217 5883
Fragments arranged by size:
5883 1884 1217 33
ScaI AGT'ACT
Cuts at: 8534 8534
Size: 9017
SexAI A'CCwGG_T
Cuts at: 1991 1991
Size: 9017
SfcI C'TryA_G
Cuts at: 33 597 760 1552 1648 2493 2638 2746 2779
Size: 564 163 792 96 845 145 108 33
Cuts at: 2779 2926 3010 3835 3886 3895 3958 4054 4075
Size: 147 84 825 51 9 63 96 21
Cuts at: 4075 4378 4768 4810 4843 5053 5395 5506 5671
Size: 303 390 42 33 210 342 111 165
Cuts at: 5671 5812 6060 6745 7738 7929 33
Size: 141 248 685 993 191 1121
Fragments arranged by size:
1121 993 845 825 792 685 564 390
342 303 248 210 191 165 163 147
145 141 111 108 96 96 84 63
51 42 33 33 21 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3391 4822 5788 3391
Size: 1431 966 6620
Fragments arranged by size:
6620 1431 966
SfoI GGC'GCC
Cuts at: 1458 5940 6449 6697 1458
Size: 4482 509 248 3778
Fragments arranged by size:
4482 3778 509 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9017
SmaI CCC'GGG
Cuts at: 1059 1691 2025 4573 6039 1059
Size: 632 334 2548 1466 4037
Fragments arranged by size:
4037 2548 1466 632 334
SmlI C'TyrA_G
Cuts at: 262 427 2218 2968 3187 3346 3433 3769 4117
Size: 165 1791 750 219 159 87 336 348
Cuts at: 4117 5494 5560 5749 6172 7087 7579 7841 262
Size: 1377 66 189 423 915 492 262 1438
Fragments arranged by size:
1791 1438 1377 915 750 492 423 348
336 262 219 189 165 159 87 66
SnaBI TAC'GTA
Cuts at: 6009 6009
Size: 9017
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9017
SphI G_CATG'C
Cuts at: 5079 6070 6570 5079
Size: 991 500 7526
Fragments arranged by size:
7526 991 500
SrfI GCCC'GGGC
Cuts at: 4573 4573
Size: 9017
StuI AGG'CCT
Cuts at: 470 784 1372 2488 5545 470
Size: 314 588 1116 3057 3942
Fragments arranged by size:
3942 3057 1116 588 314
StyI C'CwwG_G
Cuts at: 125 368 929 1091 1373 1379 2315 2621 2672
Size: 243 561 162 282 6 936 306 51
Cuts at: 2672 3098 3534 4025 4553 4589 4661 5606 6151
Size: 426 436 491 528 36 72 945 545
Cuts at: 6151 6505 125
Size: 354 2637
Fragments arranged by size:
2637 945 936 561 545 528 491 436
426 354 306 282 243 162 72 51
36 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 213 632 1009 1288 1795 5659 6600 6733 7192
Size: 419 377 279 507 3864 941 133 459
Cuts at: 7192 7401 213
Size: 209 1829
Fragments arranged by size:
3864 1829 941 507 459 419 377 279
209 133
TatI w'GTAC_w
Cuts at: 536 5537 5705 8532 536
Size: 5001 168 2827 1021
Fragments arranged by size:
5001 2827 1021 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1702 1734 3908 3940 8534 8566 1702
Size: 32 2174 32 4594 32 2153
Fragments arranged by size:
4594 2174 2153 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3946 6814 3946
Size: 2868 6149
XbaI T'CTAG_A
Cuts at: 6048 6048
Size: 9017
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 294 774 1107 1371 1581 2322 3960 4185 4668
Size: 480 333 264 210 741 1638 225 483
Cuts at: 4668 4794 6512 294
Size: 126 1718 2799
Fragments arranged by size:
2799 1718 1638 741 483 480 333 264
225 210 126
XhoI C'TCGA_G
Cuts at: 2218 3769 5560 2218
Size: 1551 1791 5675
Fragments arranged by size:
5675 1791 1551
XmaI C'CCGG_G
Cuts at: 1057 1689 2023 4571 6037 1057
Size: 632 334 2548 1466 4037
Fragments arranged by size:
4037 2548 1466 632 334
XmnI GAAnn'nnTTC
Cuts at: 4144 4144
Size: 9017
ZraI GAC'GTC
Cuts at: 2469 2469
Size: 9017
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AleI AscI AvrII BaeI BfrBI BmtI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PshAI PsiI PsrI SanDI SgrAI SspI SwaI