Restriction map for
pF1KSDB0017
(Circular) (Six-base) MAPSORT of: pF1KSDB0017.seq Check: 2059 from: 1 to: 8359
pF1KSDB0017 8359 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 774 3339 774
Size: 2565 5794
AatII G_ACGT'C
Cuts at: 2974 2974
Size: 8359
AccI GT'mk_AC
Cuts at: 2492 4871 5395 2492
Size: 2379 524 5456
Fragments arranged by size:
5456 2379 524
Acc65I G'GTAC_C
Cuts at: 5373 5373
Size: 8359
AclI AA'CG_TT
Cuts at: 8199 8199
Size: 8359
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1216 1467 1822 2263 2907 4696 6182 6614
Size: 756 251 355 441 644 1789 1486 432
Cuts at: 6614 7363 7631 460
Size: 749 268 1188
Fragments arranged by size:
1789 1486 1188 756 749 644 441 432
355 268 251
AfeI AGC'GCT
Cuts at: 3712 3893 3712
Size: 181 8178
AflIII A'CryG_T
Cuts at: 950 1876 4718 5709 6815 7792 950
Size: 926 2842 991 1106 977 1517
Fragments arranged by size:
2842 1517 1106 991 977 926
AgeI A'CCGG_T
Cuts at: 6747 6747
Size: 8359
AhdI GACnn_n'nnGTC
Cuts at: 1530 4860 1530
Size: 3330 5029
AleI CACnn'nnGTG
Cuts at: 1879 2522 5017 1879
Size: 643 2495 5221
Fragments arranged by size:
5221 2495 643
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1534 1695 1975 1534
Size: 161 280 7918
Fragments arranged by size:
7918 280 161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1390 1422 4723 4755 1390
Size: 32 3301 32 4994
Fragments arranged by size:
4994 3301 32 32
AlwNI CAG_nnn'CTG
Cuts at: 789 1458 1467 1659 3441 3684 4068 4104 5662
Size: 669 9 192 1782 243 384 36 1558
Cuts at: 5662 7231 789
Size: 1569 1917
Fragments arranged by size:
1917 1782 1569 1558 669 384 243 192
36 9
ApaI G_GGCC'C
Cuts at: 2931 3366 2931
Size: 435 7924
ApaLI G'TGCA_C
Cuts at: 5227 7129 5227
Size: 1902 6457
ApoI r'AATT_y
Cuts at: 340 649 679 5361 7712 7968 340
Size: 309 30 4682 2351 256 731
Fragments arranged by size:
4682 2351 731 309 256 30
AseI AT'TA_AT
Cuts at: 20 2443 20
Size: 2423 5936
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8359
AvaI C'yCGr_G
Cuts at: 1496 1615 2908 3246 3286 3365 3512 4090 4117
Size: 119 1293 338 40 79 147 578 27
Cuts at: 4117 4221 4246 4298 5377 1496
Size: 104 25 52 1079 4478
Fragments arranged by size:
4478 1293 1079 578 338 147 119 104
79 52 40 27 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2956 2989 2956
Size: 33 8326
BamHI G'GATC_C
Cuts at: 5382 5382
Size: 8359
BanI G'GyrC_C
Cuts at: 919 1501 2142 2380 2822 3315 3412 3508 3756
Size: 582 641 238 442 493 97 96 248
Cuts at: 3756 4903 5373 5787 6035 6070 919
Size: 1147 470 414 248 35 3208
Fragments arranged by size:
3208 1147 641 582 493 470 442 414
248 248 238 97 96 35
BanII G_rGCy'C
Cuts at: 489 881 1044 1184 1265 2931 3059 3280 3366
Size: 392 163 140 81 1666 128 221 86
Cuts at: 3366 3866 4159 4601 5276 5371 6401 489
Size: 500 293 442 675 95 1030 2447
Fragments arranged by size:
2447 1666 1030 675 500 442 392 293
221 163 140 128 95 86 81
BbeI G_GCGC'C
Cuts at: 1505 2146 2384 3319 3416 5791 6039 1505
Size: 641 238 935 97 2375 248 3825
Fragments arranged by size:
3825 2375 935 641 248 238 97
BbsI GAAGACnn'nnnn_
Cuts at: 533 1813 2104 3087 3394 3538 4235 4770 533
Size: 1280 291 983 307 144 697 535 4122
Fragments arranged by size:
4122 1280 983 697 535 307 291 144
BbvCI CC'TCA_GC
Cuts at: 3501 3501
Size: 8359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3981 4015 3981
Size: 34 8325
BciVI GTATCCnnnnn_n'
Cuts at: 2957 6255 7018 8359 2957
Size: 3298 763 1341 2957
Fragments arranged by size:
3298 2957 1341 763
BclI T'GATC_A
Cuts at: 5877 5877
Size: 8359
BglI GCCn_nnn'nGGC
Cuts at: 402 2641 3700 3772 4216 4847 402
Size: 2239 1059 72 444 631 3914
Fragments arranged by size:
3914 2239 1059 631 444 72
BglII A'GATC_T
Cuts at: 5872 7911 5872
Size: 2039 6320
BlpI GC'TnA_GC
Cuts at: 3640 3707 4153 5469 3640
Size: 67 446 1316 6530
Fragments arranged by size:
6530 1316 446 67
Bme1580I G_kGCm'C
Cuts at: 2180 2931 3366 3557 5231 5982 6075 7133 2180
Size: 751 435 191 1674 751 93 1058 3406
Fragments arranged by size:
3406 1674 1058 751 751 435 191 93
BmgBI CAC'GTC
Cuts at: 1527 2799 3423 4143 4719 4812 1527
Size: 1272 624 720 576 93 5074
Fragments arranged by size:
5074 1272 720 624 576 93
BmrI ACTGGGnnnn_n'
Cuts at: 196 1647 3429 4277 4409 5569 5650 5985 8131
Size: 1451 1782 848 132 1160 81 335 2146
Cuts at: 8131 196
Size: 424
Fragments arranged by size:
2146 1782 1451 1160 848 424 335 132
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2103 3294 3516 4641 6724 2103
Size: 1191 222 1125 2083 3738
Fragments arranged by size:
3738 2083 1191 1125 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 565 1168 1225 2382 2386 2521 2677 3073
Size: 135 603 57 1157 4 135 156 396
Cuts at: 3073 3264 3349 3443 3649 3927 5249 430
Size: 191 85 94 206 278 1322 3540
Fragments arranged by size:
3540 1322 1157 603 396 278 206 191
156 135 135 94 85 57 4
Bpu10I CC'TnA_GC
Cuts at: 1264 3501 4069 1264
Size: 2237 568 5554
Fragments arranged by size:
5554 2237 568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5533 6412 6906 7204 5533
Size: 879 494 298 6688
Fragments arranged by size:
6688 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 780 1337 2946 3131 8063 780
Size: 557 1609 185 4932 1076
Fragments arranged by size:
4932 1609 1076 557 185
BsaAI yAC'GTr
Cuts at: 4935 5349 5710 4935
Size: 414 361 7584
Fragments arranged by size:
7584 414 361
BsaBI GATnn'nnATC
Cuts at: 6 5871 7910 6
Size: 5865 2039 455
Fragments arranged by size:
5865 2039 455
BsaHI Gr'CG_yC
Cuts at: 794 1502 2143 2381 2971 3316 3413 4358 5788
Size: 708 641 238 590 345 97 945 1430
Cuts at: 5788 6036 6740 8236 794
Size: 248 704 1496 917
Fragments arranged by size:
1496 1430 945 917 708 704 641 590
345 248 238 97
BsaWI w'CCGG_w
Cuts at: 299 746 2837 3590 4882 5546 6067 6747 7021
Size: 447 2091 753 1292 664 521 680 274
Cuts at: 7021 7168 7579 299
Size: 147 411 1079
Fragments arranged by size:
2091 1292 1079 753 680 664 521 447
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1291 1321 1390 1420 1774 1804 2115 2145 2650
Size: 30 69 30 354 30 311 30 505
Cuts at: 2650 2680 2925 2955 3082 3112 3244 3274 3793
Size: 30 245 30 127 30 132 30 519
Cuts at: 3793 3823 3943 3973 4297 4327 4864 4894 1291
Size: 30 120 30 324 30 537 30 4756
Fragments arranged by size:
4756 537 519 505 354 324 311 245
132 127 120 69 30 30 30 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1019 1315 1582 1585 2108 3642 3776 3841 3967
Size: 296 267 3 523 1534 134 65 126
Cuts at: 3967 3988 4005 4074 4513 5307 1019
Size: 21 17 69 439 794 4071
Fragments arranged by size:
4071 1534 794 523 439 296 267 134
126 69 65 21 17 3
BseYI C'CCAG_C
Cuts at: 2166 3340 3572 5212 5782 6540 7119 7615 2166
Size: 1174 232 1640 570 758 579 496 2910
Fragments arranged by size:
2910 1640 1174 758 579 570 496 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1987 3458 3544 4537 5004 5059 5240 472
Size: 1515 1471 86 993 467 55 181 3591
Fragments arranged by size:
3591 1515 1471 993 467 181 86 55
BsiEI CG_ry'CG
Cuts at: 104 380 406 3229 4513 5899 5945 7155 104
Size: 276 26 2823 1284 1386 46 1210 1308
Fragments arranged by size:
2823 1386 1308 1284 1210 276 46 26
BsiHKAI G_wGCw'C
Cuts at: 353 881 1184 1265 2291 2329 3059 3280 3725
Size: 528 303 81 1026 38 730 221 445
Cuts at: 3725 4601 5231 5371 6149 6339 7133 353
Size: 876 630 140 778 190 794 1579
Fragments arranged by size:
1579 1026 876 794 778 730 630 528
445 303 221 190 140 81 38
BsmBI CGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 8359
Bsp1286I G_dGCh'C
Cuts at: 353 489 881 1044 1184 1265 2180 2291 2329
Size: 136 392 163 140 81 915 111 38
Cuts at: 2329 2931 3059 3280 3366 3557 3725 3866 4159
Size: 602 128 221 86 191 168 141 293
Cuts at: 4159 4601 5231 5276 5371 5982 6075 6149 6339
Size: 442 630 45 95 611 93 74 190
Cuts at: 6339 6401 7133 353
Size: 62 732 1579
Fragments arranged by size:
1579 915 732 630 611 602 442 392
293 221 191 190 168 163 141 140
136 128 111 95 93 86 81 74
62 45 38
BspEI T'CCGG_A
Cuts at: 299 746 2837 3590 7579 299
Size: 447 2091 753 3989 1079
Fragments arranged by size:
3989 2091 1079 753 447
BspHI T'CATG_A
Cuts at: 7535 7535
Size: 8359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 774 1466 3339 3917 5054 5407 5621 5923 6304
Size: 692 1873 578 1137 353 214 302 381
Cuts at: 6304 774
Size: 2829
Fragments arranged by size:
2829 1873 1137 692 578 381 353 302
214
BsrBI CCG'CTC
Cuts at: 50 402 1736 2370 3639 4277 4509 5111 6649
Size: 352 1334 634 1269 638 232 602 1538
Cuts at: 6649 6703 8189 8354 50
Size: 54 1486 165 55
Fragments arranged by size:
1538 1486 1334 1269 638 634 602 352
232 165 55 54
BsrDI GCAATG_nn'
Cuts at: 6269 6269
Size: 8359
BsrFI r'CCGG_y
Cuts at: 819 1046 3318 3426 3759 4028 4148 4661 6355
Size: 227 2272 108 333 269 120 513 1694
Cuts at: 6355 6536 6747 819
Size: 181 211 2431
Fragments arranged by size:
2431 2272 1694 513 333 269 227 211
181 120 108
BsrGI T'GTAC_A
Cuts at: 1850 3740 1850
Size: 1890 6469
BssSI C'ACGA_G
Cuts at: 6628 6988 6628
Size: 360 7999
BstAPI GCAn_nnn'nTGC
Cuts at: 426 4104 5734 426
Size: 3678 1630 3051
Fragments arranged by size:
3678 3051 1630
BstBI TT'CG_AA
Cuts at: 110 110
Size: 8359
BstXI CCAn_nnnn'nTGG
Cuts at: 4812 4812
Size: 8359
BstYI r'GATC_y
Cuts at: 1 384 3581 4532 5183 5382 5849 5872 6207
Size: 383 3197 951 651 199 467 23 335
Cuts at: 6207 6453 7467 7553 7565 7911 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
3197 1014 951 651 467 449 383 346
335 246 199 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 4160 4160
Size: 8359
BtgI C'CryG_G
Cuts at: 125 248 797 1103 1292 1326 2420 2708 3014
Size: 123 549 306 189 34 1094 288 306
Cuts at: 3014 3357 3396 3466 3815 125
Size: 343 39 70 349 4669
Fragments arranged by size:
4669 1094 549 349 343 306 306 288
189 123 70 39 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 693 4770 6307 6488 6651 693
Size: 4077 1537 181 163 2401
Fragments arranged by size:
4077 2401 1537 181 163
BtsI GCAGTG_nn'
Cuts at: 362 628 1999 2215 2316 2650 3453 4987 5600
Size: 266 1371 216 101 334 803 1534 613
Cuts at: 5600 362
Size: 3121
Fragments arranged by size:
3121 1534 1371 803 613 334 266 216
101
DraI TTT'AAA
Cuts at: 5356 5356
Size: 8359
DraIII CAC_nnn'GTG
Cuts at: 357 426 357
Size: 69 8290
DrdI GACnn_nn'nnGTC
Cuts at: 772 799 1633 6063 6923 772
Size: 27 834 4430 860 2208
Fragments arranged by size:
4430 2208 860 834 27
EaeI y'GGCC_r
Cuts at: 128 403 1433 1646 1775 2417 2642 2675 3320
Size: 275 1030 213 129 642 225 33 645
Cuts at: 3320 3773 4026 4734 5021 5942 6116 6507 6534
Size: 453 253 708 287 921 174 391 27
Cuts at: 6534 7881 128
Size: 1347 606
Fragments arranged by size:
1347 1030 921 708 645 642 606 453
391 287 275 253 225 213 174 129
33 27
EagI C'GGCC_G
Cuts at: 403 5942 403
Size: 5539 2820
EarI CTCTTCn'nnn_
Cuts at: 341 496 593 2197 3115 6380 6590 341
Size: 155 97 1604 918 3265 210 2110
Fragments arranged by size:
3265 2110 1604 918 210 155 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3592 3801 5146 5164 6565 6877 7023 8169 3592
Size: 209 1345 18 1401 312 146 1146 3782
Fragments arranged by size:
3782 1401 1345 1146 312 209 146 18
EcoICRI GAG'CTC
Cuts at: 879 1182 1263 3057 3278 4599 5369 879
Size: 303 81 1794 221 1321 770 3869
Fragments arranged by size:
3869 1794 1321 770 303 221 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 460 565 1168 1216 1225 1467 1822 2263
Size: 30 105 603 48 9 242 355 441
Cuts at: 2263 2382 2386 2521 2677 2907 3073 3264 3349
Size: 119 4 135 156 230 166 191 85
Cuts at: 3349 3443 3649 3927 4696 5249 6182 6614 7363
Size: 94 206 278 769 553 933 432 749
Cuts at: 7363 7631 430
Size: 268 1158
Fragments arranged by size:
1158 933 769 749 603 553 441 432
355 278 268 242 230 206 191 166
156 135 119 105 94 85 48 30
9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 964 1672 2123 2503 3924 964
Size: 708 451 380 1421 5399
Fragments arranged by size:
5399 1421 708 451 380
EcoO109I rG'GnC_Cy
Cuts at: 477 558 2128 2152 2508 2868 2927 3221 3362
Size: 81 1570 24 356 360 59 294 141
Cuts at: 3362 4122 4187 4252 5001 5496 477
Size: 760 65 65 749 495 3340
Fragments arranged by size:
3340 1570 760 749 495 360 356 294
141 81 65 65 59 24
EcoRI G'AATT_C
Cuts at: 649 5361 649
Size: 4712 3647
EcoRV GAT'ATC
Cuts at: 5127 5127
Size: 8359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2937 6376 2937
Size: 3439 4920
FseI GG_CCGG'CC
Cuts at: 4032 4032
Size: 8359
FspI TGC'GCA
Cuts at: 1510 6138 1510
Size: 4628 3731
FspAI rTGC'GCAy
Cuts at: 1510 1510
Size: 8359
HaeII r_GCGC'y
Cuts at: 941 1468 1505 2146 2384 2593 3319 3416 3714
Size: 527 37 641 238 209 726 97 298
Cuts at: 3714 3895 4842 5310 5791 6039 7063 8042 941
Size: 181 947 468 481 248 1024 979 1258
Fragments arranged by size:
1258 1024 979 947 726 641 527 481
468 298 248 238 209 181 97 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 717 749 1111 1143 1516 1548 2650
Size: 32 496 32 362 32 373 32 1102
Cuts at: 2650 2682 2925 2957 2995 3027 3244 3276 3943
Size: 32 243 32 38 32 217 32 667
Cuts at: 3943 3975 4483 4515 4846 4878 6199 6229 6231
Size: 32 508 32 331 32 1321 30 2
Cuts at: 6231 6261 189
Size: 30 2287
Fragments arranged by size:
2287 1321 1102 667 508 496 373 362
331 243 217 38 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1289 5396 1289
Size: 4107 4252
HindIII A'AGCT_T
Cuts at: 3187 4897 3187
Size: 1710 6649
KasI G'GCGC_C
Cuts at: 1501 2142 2380 3315 3412 5787 6035 1501
Size: 641 238 935 97 2375 248 3825
Fragments arranged by size:
3825 2375 935 641 248 238 97
KpnI G_GTAC'C
Cuts at: 5377 5377
Size: 8359
MluI A'CGCG_T
Cuts at: 1876 7792 1876
Size: 5916 2443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 545 3125 3824 3854 4340 4554 5138 5231 7030
Size: 2580 699 30 486 214 584 93 1799
Cuts at: 7030 7214 545
Size: 184 1690
Fragments arranged by size:
2580 1799 1690 699 584 486 214 184
93 30
MscI TGG'CCA
Cuts at: 130 1648 2677 5023 6118 130
Size: 1518 1029 2346 1095 2371
Fragments arranged by size:
2371 2346 1518 1095 1029
MslI CAynn'nnrTG
Cuts at: 301 1516 1879 2522 2806 4795 5017 6346 6473
Size: 1215 363 643 284 1989 222 1329 127
Cuts at: 6473 301
Size: 2187
Fragments arranged by size:
2187 1989 1329 1215 643 363 284 222
127
MspA1I CmG'CkG
Cuts at: 250 408 837 2105 2322 2522 2710 3505 3614
Size: 158 429 1268 217 200 188 795 109
Cuts at: 3614 4826 4950 5181 5301 5468 5782 6142 7157
Size: 1212 124 231 120 167 314 360 1015
Cuts at: 7157 7402 250
Size: 245 1207
Fragments arranged by size:
1268 1212 1207 1015 795 429 360 314
245 231 217 200 188 167 158 124
120 109
NaeI GCC'GGC
Cuts at: 821 3320 3428 3761 4030 4150 4663 6538 821
Size: 2499 108 333 269 120 513 1875 2642
Fragments arranged by size:
2642 2499 1875 513 333 269 120 108
NarI GG'CG_CC
Cuts at: 1502 2143 2381 3316 3413 5788 6036 1502
Size: 641 238 935 97 2375 248 3825
Fragments arranged by size:
3825 2375 935 641 248 238 97
NcoI C'CATG_G
Cuts at: 125 1103 3396 125
Size: 978 2293 5088
Fragments arranged by size:
5088 2293 978
NgoMIV G'CCGG_C
Cuts at: 819 3318 3426 3759 4028 4148 4661 6536 819
Size: 2499 108 333 269 120 513 1875 2642
Fragments arranged by size:
2642 2499 1875 513 333 269 120 108
NotI GC'GGCC_GC
Cuts at: 403 403
Size: 8359
NruI TCG'CGA
Cuts at: 1945 6720 1945
Size: 4775 3584
NspI r_CATG'y
Cuts at: 954 1112 3298 3672 5410 5910 6819 954
Size: 158 2186 374 1738 500 909 2494
Fragments arranged by size:
2494 2186 1738 909 500 374 158
PasI CC'CwG_GG
Cuts at: 3567 3868 4126 3567
Size: 301 258 7800
Fragments arranged by size:
7800 301 258
PciI A'CATG_T
Cuts at: 950 6815 950
Size: 5865 2494
PflMI CCAn_nnn'nTGG
Cuts at: 963 1104 1191 1248 1659 3170 3441 963
Size: 141 87 57 411 1511 271 5881
Fragments arranged by size:
5881 1511 411 271 141 87 57
PfoI T'CCnGG_A
Cuts at: 3459 3459
Size: 8359
PmeI GTTT'AAAC
Cuts at: 5356 5356
Size: 8359
PmlI CAC'GTG
Cuts at: 4935 4935
Size: 8359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1390 1422 7498 7530 1390
Size: 32 6076 32 2219
Fragments arranged by size:
6076 2219 32 32
PpuMI rG'GwC_Cy
Cuts at: 477 3221 4122 4187 4252 477
Size: 2744 901 65 65 4584
Fragments arranged by size:
4584 2744 901 65 65
PshAI GACnn'nnGTC
Cuts at: 1273 1813 1273
Size: 540 7819
PspOMI G'GGCC_C
Cuts at: 2927 3362 2927
Size: 435 7924
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4627 4659 4627
Size: 32 8327
PstI C_TGCA'G
Cuts at: 219 623 785 1463 1964 2151 2210 2560 3003
Size: 404 162 678 501 187 59 350 443
Cuts at: 3003 3260 3680 4100 4109 4433 4982 5404 6089
Size: 257 420 420 9 324 549 422 685
Cuts at: 6089 219
Size: 2489
Fragments arranged by size:
2489 685 678 549 501 443 422 420
420 404 350 324 257 187 162 59
9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8359
PvuII CAG'CTG
Cuts at: 2322 3505 3614 5782 6142 2322
Size: 1183 109 2168 360 4539
Fragments arranged by size:
4539 2168 1183 360 109
RsrII CG'GwC_CG
Cuts at: 749 6552 749
Size: 5803 2556
SacI G_AGCT'C
Cuts at: 881 1184 1265 3059 3280 4601 5371 881
Size: 303 81 1794 221 1321 770 3869
Fragments arranged by size:
3869 1794 1321 770 303 221 81
SacII CC_GC'GG
Cuts at: 251 2711 251
Size: 2460 5899
SalI G'TCGA_C
Cuts at: 5394 5394
Size: 8359
SanDI GG'GwC_CC
Cuts at: 3221 4122 4187 4252 3221
Size: 901 65 65 7328
Fragments arranged by size:
7328 901 65 65
SapI GCTCTTCn'nnn_
Cuts at: 341 593 2197 6380 6590 341
Size: 252 1604 4183 210 2110
Fragments arranged by size:
4183 2110 1604 252 210
SbfI CC_TGCA'GG
Cuts at: 785 1463 2151 3680 5404 785
Size: 678 688 1529 1724 3740
Fragments arranged by size:
3740 1724 1529 688 678
ScaI AGT'ACT
Cuts at: 2937 7876 2937
Size: 4939 3420
SexAI A'CCwGG_T
Cuts at: 1631 4967 1631
Size: 3336 5023
SfcI C'TryA_G
Cuts at: 33 215 619 781 1410 1459 1960 2147 2206
Size: 182 404 162 629 49 501 187 59
Cuts at: 2206 2556 2694 2999 3256 3676 3921 4096 4105
Size: 350 138 305 257 420 245 175 9
Cuts at: 4105 4429 4629 4978 5400 6085 7080 7271 33
Size: 324 200 349 422 685 995 191 1121
Fragments arranged by size:
1121 995 685 629 501 422 420 404
350 349 324 305 257 245 200 191
187 182 175 162 138 59 49 9
SfoI GGC'GCC
Cuts at: 1503 2144 2382 3317 3414 5789 6037 1503
Size: 641 238 935 97 2375 248 3825
Fragments arranged by size:
3825 2375 935 641 248 238 97
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8359
SmaI CCC'GGG
Cuts at: 3288 3367 3514 5379 3288
Size: 79 147 1865 6268
Fragments arranged by size:
6268 1865 147 79
SmlI C'TyrA_G
Cuts at: 5512 6427 6921 7183 5512
Size: 915 494 262 6688
Fragments arranged by size:
6688 915 494 262
SnaBI TAC'GTA
Cuts at: 5349 5349
Size: 8359
SpeI A'CTAG_T
Cuts at: 67 2938 67
Size: 2871 5488
SphI G_CATG'C
Cuts at: 5410 5910 5410
Size: 500 7859
StuI AGG'CCT
Cuts at: 1380 1966 3239 3499 4712 1380
Size: 586 1273 260 1213 5027
Fragments arranged by size:
5027 1273 1213 586 260
StyI C'CwwG_G
Cuts at: 125 915 1103 1247 1436 1793 3308 3396 4805
Size: 790 188 144 189 357 1515 88 1409
Cuts at: 4805 5491 5845 125
Size: 686 354 2639
Fragments arranged by size:
2639 1515 1409 790 686 357 354 189
188 144 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 937 1666 4564 5940 6073 6532 6743 937
Size: 729 2898 1376 133 459 211 2553
Fragments arranged by size:
2898 2553 1376 729 459 211 133
TatI w'GTAC_w
Cuts at: 704 1850 2774 2935 3740 7874 704
Size: 1146 924 161 805 4134 1189
Fragments arranged by size:
4134 1189 1146 924 805 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1291 1323 2115 2147 2308 2340 3501 3533 4945
Size: 32 792 32 161 32 1161 32 1412
Cuts at: 4945 4977 5221 5253 7876 7908 1291
Size: 32 244 32 2623 32 1742
Fragments arranged by size:
2623 1742 1412 1161 792 244 161 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 613 753 3225 6154 613
Size: 140 2472 2929 2818
Fragments arranged by size:
2929 2818 2472 140
XbaI T'CTAG_A
Cuts at: 5388 5388
Size: 8359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1656 2559 2637 2988 3171 4809 5220 5852 1656
Size: 903 78 351 183 1638 411 632 4163
Fragments arranged by size:
4163 1638 903 632 411 351 183 78
XmaI C'CCGG_G
Cuts at: 3286 3365 3512 5377 3286
Size: 79 147 1865 6268
Fragments arranged by size:
6268 1865 147 79
XmnI GAAnn'nnTTC
Cuts at: 530 653 1321 530
Size: 123 668 7568
Fragments arranged by size:
7568 668 123
ZraI GAC'GTC
Cuts at: 2972 2972
Size: 8359
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BfrBI BmtI BsiWI BsmI BssHII
BstEII BstZ17I ClaI CspCI HpaI MfeI NdeI NheI
NsiI PacI PsiI PspXI SfiI SgrAI SrfI SspI
SwaI XhoI