Restriction map for
pF1KSDB0022
(Circular) (Six-base) MAPSORT of: pF1KSDB0022.seq Check: 9320 from: 1 to: 4699
pF1KSDB0022 4699 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1033 1735 1033
Size: 702 3997
Acc65I G'GTAC_C
Cuts at: 1713 1713
Size: 4699
AclI AA'CG_TT
Cuts at: 4539 4539
Size: 4699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2522 2954 3703 3971 2522
Size: 432 749 268 3250
Fragments arranged by size:
3250 749 432 268
AflIII A'CryG_T
Cuts at: 2049 3155 4132 2049
Size: 1106 977 2616
Fragments arranged by size:
2616 1106 977
AgeI A'CCGG_T
Cuts at: 1449 3087 1449
Size: 1638 3061
AleI CACnn'nnGTG
Cuts at: 1054 1397 1054
Size: 343 4356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1402 1402
Size: 4699
AlwNI CAG_nnn'CTG
Cuts at: 1633 2002 3571 1633
Size: 369 1569 2761
Fragments arranged by size:
2761 1569 369
ApaI G_GGCC'C
Cuts at: 1601 1601
Size: 4699
ApaLI G'TGCA_C
Cuts at: 3469 3469
Size: 4699
ApoI r'AATT_y
Cuts at: 1701 4052 4308 1701
Size: 2351 256 2092
Fragments arranged by size:
2351 2092 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4699
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4699
AvaI C'yCGr_G
Cuts at: 421 809 1374 1717 421
Size: 388 565 343 3403
Fragments arranged by size:
3403 565 388 343
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1664 1697 1664
Size: 33 4666
BamHI G'GATC_C
Cuts at: 1552 1722 1552
Size: 170 4529
BanI G'GyrC_C
Cuts at: 561 1713 2127 2375 2410 561
Size: 1152 414 248 35 2850
Fragments arranged by size:
2850 1152 414 248 35
BanII G_rGCy'C
Cuts at: 428 457 497 506 583 915 1157 1601 1648
Size: 29 40 9 77 332 242 444 47
Cuts at: 1648 1711 2741 428
Size: 63 1030 2386
Fragments arranged by size:
2386 1030 444 332 242 77 63 47
40 29 9
BbeI G_GCGC'C
Cuts at: 2131 2379 2131
Size: 248 4451
BbvCI CC'TCA_GC
Cuts at: 1307 1328 1640 1307
Size: 21 312 4366
Fragments arranged by size:
4366 312 21
BciVI GTATCCnnnnn_n'
Cuts at: 2595 3358 4699 2595
Size: 763 1341 2595
Fragments arranged by size:
2595 1341 763
BclI T'GATC_A
Cuts at: 2217 2217
Size: 4699
BfrBI ATG'CAT
Cuts at: 972 972
Size: 4699
BglI GCCn_nnn'nGGC
Cuts at: 717 717
Size: 4699
BglII A'GATC_T
Cuts at: 996 2212 4251 996
Size: 1216 2039 1444
Fragments arranged by size:
2039 1444 1216
BlpI GC'TnA_GC
Cuts at: 229 1647 1809 229
Size: 1418 162 3119
Fragments arranged by size:
3119 1418 162
Bme1580I G_kGCm'C
Cuts at: 566 1601 2322 2415 3473 566
Size: 1035 721 93 1058 1792
Fragments arranged by size:
1792 1058 1035 721 93
BmrI ACTGGGnnnn_n'
Cuts at: 1023 1048 1615 1909 1990 2325 4471 1023
Size: 25 567 294 81 335 2146 1251
Fragments arranged by size:
2146 1251 567 335 294 81 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1176 3064 1176
Size: 1888 2811
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1475 484
Size: 991 3708
Bpu10I CC'TnA_GC
Cuts at: 1307 1328 1517 1640 1307
Size: 21 189 123 4366
Fragments arranged by size:
4366 189 123 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1873 2752 3246 3544 310
Size: 1563 879 494 298 1465
Fragments arranged by size:
1563 1465 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 376 1029 1537 1562 4403 376
Size: 653 508 25 2841 672
Fragments arranged by size:
2841 672 653 508 25
BsaAI yAC'GTr
Cuts at: 1395 1689 2050 1395
Size: 294 361 4044
Fragments arranged by size:
4044 361 294
BsaBI GATnn'nnATC
Cuts at: 6 145 2211 4250 6
Size: 139 2066 2039 455
Fragments arranged by size:
2066 2039 455 139
BsaHI Gr'CG_yC
Cuts at: 2128 2376 3080 4576 2128
Size: 248 704 1496 2251
Fragments arranged by size:
2251 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1449 1886 2407 3087 3361 3508 3919 1449
Size: 437 521 680 274 147 411 2229
Fragments arranged by size:
2229 680 521 437 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 457 487 457
Size: 30 4669
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 505 817 1149 505
Size: 312 332 4055
Fragments arranged by size:
4055 332 312
BseYI C'CCAG_C
Cuts at: 601 668 713 741 778 902 916 1010 1235
Size: 67 45 28 37 124 14 94 225
Cuts at: 1235 1503 1542 1620 2122 2880 3459 3955 601
Size: 268 39 78 502 758 579 496 1345
Fragments arranged by size:
1345 758 579 502 496 268 225 124
94 78 67 45 39 37 28 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1169 1169
Size: 4699
BsiEI CG_ry'CG
Cuts at: 104 2239 2285 3495 104
Size: 2135 46 1210 1308
Fragments arranged by size:
2135 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 497 583 810 1157 1711 2489 2679 3473 497
Size: 86 227 347 554 778 190 794 1723
Fragments arranged by size:
1723 794 778 554 347 227 190 86
BsmBI CGTCTCn'nnnn_
Cuts at: 1555 1555
Size: 4699
Bsp1286I G_dGCh'C
Cuts at: 428 457 497 506 566 583 810 915 1157
Size: 29 40 9 60 17 227 105 242
Cuts at: 1157 1601 1648 1711 2322 2415 2489 2679 2741
Size: 444 47 63 611 93 74 190 62
Cuts at: 2741 3473 428
Size: 732 1654
Fragments arranged by size:
1654 732 611 444 242 227 190 105
93 74 63 62 60 47 40 29
17 9
BspEI T'CCGG_A
Cuts at: 3919 3919
Size: 4699
BspHI T'CATG_A
Cuts at: 3875 3875
Size: 4699
BspMI ACCTGCnnnn'nnnn_
Cuts at: 941 1747 1961 2263 2644 941
Size: 806 214 302 381 2996
Fragments arranged by size:
2996 806 381 302 214
BsrBI CCG'CTC
Cuts at: 50 168 2989 3043 4529 4694 50
Size: 118 2821 54 1486 165 55
Fragments arranged by size:
2821 1486 165 118 55 54
BsrDI GCAATG_nn'
Cuts at: 1209 2609 1209
Size: 1400 3299
BsrFI r'CCGG_y
Cuts at: 1449 2695 2876 3087 1449
Size: 1246 181 211 3061
Fragments arranged by size:
3061 1246 211 181
BssSI C'ACGA_G
Cuts at: 352 2968 3328 352
Size: 2616 360 1723
Fragments arranged by size:
2616 1723 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2074 2074
Size: 4699
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4699
BstEII G'GTnAC_C
Cuts at: 615 748 615
Size: 133 4566
BstXI CCAn_nnnn'nTGG
Cuts at: 1018 1056 1018
Size: 38 4661
BstYI r'GATC_y
Cuts at: 1 146 996 1552 1722 2189 2212 2547 2793
Size: 145 850 556 170 467 23 335 246
Cuts at: 2793 3807 3893 3905 4251 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 850 556 467 449 346 335 246
170 145 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 388 1634 388
Size: 1246 3453
BtgI C'CryG_G
Cuts at: 125 652 125
Size: 527 4172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1541 2647 2828 2991 1541
Size: 1106 181 163 3249
Fragments arranged by size:
3249 1106 181 163
BtsI GCAGTG_nn'
Cuts at: 583 1115 1208 1940 583
Size: 532 93 732 3342
Fragments arranged by size:
3342 732 532 93
DraI TTT'AAA
Cuts at: 1696 1696
Size: 4699
DrdI GACnn_nn'nnGTC
Cuts at: 937 2403 3263 937
Size: 1466 860 2373
Fragments arranged by size:
2373 1466 860
EaeI y'GGCC_r
Cuts at: 718 836 2282 2456 2847 2874 4221 718
Size: 118 1446 174 391 27 1347 1196
Fragments arranged by size:
1446 1347 1196 391 174 118 27
EagI C'GGCC_G
Cuts at: 2282 2282
Size: 4699
EarI CTCTTCn'nnn_
Cuts at: 511 1110 1651 2720 2930 511
Size: 599 541 1069 210 2280
Fragments arranged by size:
2280 1069 599 541 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 869 2905 3217 3363 4509 869
Size: 2036 312 146 1146 1059
Fragments arranged by size:
2036 1146 1059 312 146
EcoICRI GAG'CTC
Cuts at: 495 581 1155 1709 495
Size: 86 574 554 3485
Fragments arranged by size:
3485 574 554 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1475 2522 2954 3703 3971 484
Size: 991 1047 432 749 268 1212
Fragments arranged by size:
1212 1047 991 749 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 463 856 463
Size: 393 4306
EcoO109I rG'GnC_Cy
Cuts at: 1092 1836 1092
Size: 744 3955
EcoRI G'AATT_C
Cuts at: 1701 1701
Size: 4699
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 417 2716 417
Size: 2299 2400
FspI TGC'GCA
Cuts at: 2478 2478
Size: 4699
HaeII r_GCGC'y
Cuts at: 516 1017 2131 2379 3403 4382 516
Size: 501 1114 248 1024 979 833
Fragments arranged by size:
1114 1024 979 833 501 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2539 2569 2571 2601 2539
Size: 30 2 30 4637
Fragments arranged by size:
4637 30 30 2
HincII GTy'rAC
Cuts at: 524 1142 1736 524
Size: 618 594 3487
Fragments arranged by size:
3487 618 594
KasI G'GCGC_C
Cuts at: 2127 2375 2127
Size: 248 4451
KpnI G_GTAC'C
Cuts at: 1717 1717
Size: 4699
MluI A'CGCG_T
Cuts at: 4132 4132
Size: 4699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 302 3370 3554 302
Size: 3068 184 1447
Fragments arranged by size:
3068 1447 184
MscI TGG'CCA
Cuts at: 838 2458 838
Size: 1620 3079
MslI CAynn'nnrTG
Cuts at: 307 1054 1397 2686 2813 307
Size: 747 343 1289 127 2193
Fragments arranged by size:
2193 1289 747 343 127
MspA1I CmG'CkG
Cuts at: 605 654 717 1495 1507 1651 1808 2122 2482
Size: 49 63 778 12 144 157 314 360
Cuts at: 2482 3497 3742 605
Size: 1015 245 1562
Fragments arranged by size:
1562 1015 778 360 314 245 157 144
63 49 12
NaeI GCC'GGC
Cuts at: 2878 2878
Size: 4699
NarI GG'CG_CC
Cuts at: 2128 2376 2128
Size: 248 4451
NcoI C'CATG_G
Cuts at: 125 125
Size: 4699
NgoMIV G'CCGG_C
Cuts at: 2876 2876
Size: 4699
NruI TCG'CGA
Cuts at: 3060 3060
Size: 4699
NsiI A_TGCA'T
Cuts at: 974 974
Size: 4699
NspI r_CATG'y
Cuts at: 1750 2250 3159 1750
Size: 500 909 3290
Fragments arranged by size:
3290 909 500
PciI A'CATG_T
Cuts at: 3155 3155
Size: 4699
PmeI GTTT'AAAC
Cuts at: 1696 1696
Size: 4699
PmlI CAC'GTG
Cuts at: 1395 1395
Size: 4699
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3838 3870 3838
Size: 32 4667
PspOMI G'GGCC_C
Cuts at: 1597 1597
Size: 4699
PstI C_TGCA'G
Cuts at: 590 1744 2429 590
Size: 1154 685 2860
Fragments arranged by size:
2860 1154 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4699
PvuII CAG'CTG
Cuts at: 1495 1507 1651 2122 2482 1495
Size: 12 144 471 360 3712
Fragments arranged by size:
3712 471 360 144 12
RsrII CG'GwC_CG
Cuts at: 2892 2892
Size: 4699
SacI G_AGCT'C
Cuts at: 497 583 1157 1711 497
Size: 86 574 554 3485
Fragments arranged by size:
3485 574 554 86
SacII CC_GC'GG
Cuts at: 655 655
Size: 4699
SalI G'TCGA_C
Cuts at: 1734 1734
Size: 4699
SapI GCTCTTCn'nnn_
Cuts at: 511 2720 2930 511
Size: 2209 210 2280
Fragments arranged by size:
2280 2209 210
SbfI CC_TGCA'GG
Cuts at: 1744 1744
Size: 4699
ScaI AGT'ACT
Cuts at: 4216 4216
Size: 4699
SexAI A'CCwGG_T
Cuts at: 1343 1343
Size: 4699
SfcI C'TryA_G
Cuts at: 33 586 682 1740 2425 3420 3611 33
Size: 553 96 1058 685 995 191 1121
Fragments arranged by size:
1121 1058 995 685 553 191 96
SfoI GGC'GCC
Cuts at: 2129 2377 2129
Size: 248 4451
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4699
SmaI CCC'GGG
Cuts at: 1376 1719 1376
Size: 343 4356
SmlI C'TyrA_G
Cuts at: 325 1852 2767 3261 3523 325
Size: 1527 915 494 262 1501
Fragments arranged by size:
1527 1501 915 494 262
SnaBI TAC'GTA
Cuts at: 1689 1689
Size: 4699
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4699
SphI G_CATG'C
Cuts at: 1750 2250 1750
Size: 500 4199
StyI C'CwwG_G
Cuts at: 125 1017 1831 2185 125
Size: 892 814 354 2639
Fragments arranged by size:
2639 892 814 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 916 1387 1435 2280 2413 2872 3083 916
Size: 471 48 845 133 459 211 2532
Fragments arranged by size:
2532 845 471 459 211 133 48
TatI w'GTAC_w
Cuts at: 329 338 4214 329
Size: 9 3876 814
Fragments arranged by size:
3876 814 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4216 4248 4216
Size: 32 4667
Tth111I GACn'n_nGTC
Cuts at: 2494 2494
Size: 4699
XbaI T'CTAG_A
Cuts at: 1728 1728
Size: 4699
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 609 2192 306
Size: 303 1583 2813
Fragments arranged by size:
2813 1583 303
XmaI C'CCGG_G
Cuts at: 1374 1717 1374
Size: 343 4356
XmnI GAAnn'nnTTC
Cuts at: 400 400
Size: 4699
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NsiI NspI PciI PmeI PmlI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BbsI BcgI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI PacI PasI PflMI
PfoI PpuMI PshAI PsiI PspXI PsrI SanDI SfiI
SgrAI SrfI SspI StuI SwaI XhoI ZraI