Restriction map for
pF1KSDB0024
(Circular) (Six-base) MAPSORT of: pF1KSDB0024.seq Check: 5710 from: 1 to: 6880
pF1KSDB0024 6880 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 3857 3916 3857
Size: 59 6821
Acc65I G'GTAC_C
Cuts at: 2227 3894 2227
Size: 1667 5213
AclI AA'CG_TT
Cuts at: 993 3062 6720 993
Size: 2069 3658 1153
Fragments arranged by size:
3658 2069 1153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1367 1415 2068 2180 2711 3011 3038 3681
Size: 1069 48 653 112 531 300 27 643
Cuts at: 3681 4703 5135 5884 6152 298
Size: 1022 432 749 268 1026
Fragments arranged by size:
1069 1026 1022 749 653 643 531 432
300 268 112 48 27
AflIII A'CryG_T
Cuts at: 3437 4230 5336 6313 3437
Size: 793 1106 977 4004
Fragments arranged by size:
4004 1106 977 793
AgeI A'CCGG_T
Cuts at: 5268 5268
Size: 6880
AhdI GACnn_n'nnGTC
Cuts at: 874 1821 874
Size: 947 5933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1894 2986 1894
Size: 1092 5788
AlwNI CAG_nnn'CTG
Cuts at: 3165 4183 5752 3165
Size: 1018 1569 4293
Fragments arranged by size:
4293 1569 1018
ApaI G_GGCC'C
Cuts at: 3141 3141
Size: 6880
ApaLI G'TGCA_C
Cuts at: 5650 5650
Size: 6880
ApoI r'AATT_y
Cuts at: 430 2205 3882 6233 6489 430
Size: 1775 1677 2351 256 821
Fragments arranged by size:
2351 1775 1677 821 256
AseI AT'TA_AT
Cuts at: 20 152 20
Size: 132 6748
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6880
AvaI C'yCGr_G
Cuts at: 1013 2551 3898 1013
Size: 1538 1347 3995
Fragments arranged by size:
3995 1538 1347
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2211 2244 2211
Size: 33 6847
BamHI G'GATC_C
Cuts at: 829 1804 3903 829
Size: 975 2099 3806
Fragments arranged by size:
3806 2099 975
BanI G'GyrC_C
Cuts at: 2227 2908 3894 4308 4556 4591 2227
Size: 681 986 414 248 35 4516
Fragments arranged by size:
4516 986 681 414 248 35
BanII G_rGCy'C
Cuts at: 2469 3141 3892 4922 2469
Size: 672 751 1030 4427
Fragments arranged by size:
4427 1030 751 672
BbeI G_GCGC'C
Cuts at: 4312 4560 4312
Size: 248 6632
BbsI GAAGACnn'nnnn_
Cuts at: 250 1860 2612 2760 3411 250
Size: 1610 752 148 651 3719
Fragments arranged by size:
3719 1610 752 651 148
BbvCI CC'TCA_GC
Cuts at: 1779 1779
Size: 6880
BciVI GTATCCnnnnn_n'
Cuts at: 636 1518 3467 4776 5539 6880 636
Size: 882 1949 1309 763 1341 636
Fragments arranged by size:
1949 1341 1309 882 763 636
BclI T'GATC_A
Cuts at: 4398 4398
Size: 6880
BglI GCCn_nnn'nGGC
Cuts at: 2198 2198
Size: 6880
BglII A'GATC_T
Cuts at: 1702 3297 4393 6432 1702
Size: 1595 1096 2039 2150
Fragments arranged by size:
2150 2039 1595 1096
BlpI GC'TnA_GC
Cuts at: 3573 3990 3573
Size: 417 6463
Bme1580I G_kGCm'C
Cuts at: 3141 4503 4596 5654 3141
Size: 1362 93 1058 4367
Fragments arranged by size:
4367 1362 1058 93
BmgBI CAC'GTC
Cuts at: 3438 3703 3438
Size: 265 6615
BmrI ACTGGGnnnn_n'
Cuts at: 603 2276 2808 3135 4090 4171 4506 6652 603
Size: 1673 532 327 955 81 335 2146 831
Fragments arranged by size:
2146 1673 955 831 532 335 327 81
BmtI G_CTAG'C
Cuts at: 1300 2074 1300
Size: 774 6106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 358 5245 358
Size: 4887 1993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 559 3086 3735 328
Size: 231 2527 649 3473
Fragments arranged by size:
3473 2527 649 231
Bpu10I CC'TnA_GC
Cuts at: 1779 2702 1779
Size: 923 5957
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1312 2557 2720 3535 4054 4933 5427 5725
Size: 681 1245 163 815 519 879 494 298
Cuts at: 5725 631
Size: 1786
Fragments arranged by size:
1786 1245 879 815 681 519 494 298
163
BsaI GGTCTCn'nnnn_
Cuts at: 271 6584 271
Size: 6313 567
BsaAI yAC'GTr
Cuts at: 2877 3870 4231 2877
Size: 993 361 5526
Fragments arranged by size:
5526 993 361
BsaBI GATnn'nnATC
Cuts at: 6 3182 4392 6431 6
Size: 3176 1210 2039 455
Fragments arranged by size:
3176 2039 1210 455
BsaHI Gr'CG_yC
Cuts at: 3845 4309 4557 5261 6757 3845
Size: 464 248 704 1496 3968
Fragments arranged by size:
3968 1496 704 464 248
BsaWI w'CCGG_w
Cuts at: 426 3147 4067 4588 5268 5542 5689 6100 426
Size: 2721 920 521 680 274 147 411 1206
Fragments arranged by size:
2721 1206 920 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 2433 2463 193
Size: 30 2210 30 4610
Fragments arranged by size:
4610 2210 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1516 2438 2441 3023 3722 1516
Size: 922 3 582 699 4674
Fragments arranged by size:
4674 922 699 582 3
BseYI C'CCAG_C
Cuts at: 302 3134 3214 4303 5061 5640 6136 302
Size: 2832 80 1089 758 579 496 1046
Fragments arranged by size:
2832 1089 1046 758 579 496 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 719 422
Size: 297 6583
BsiEI CG_ry'CG
Cuts at: 104 4420 4466 5676 104
Size: 4316 46 1210 1308
Fragments arranged by size:
4316 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 674 1176 3892 4670 4860 5654 674
Size: 502 2716 778 190 794 1900
Fragments arranged by size:
2716 1900 794 778 502 190
BsmI GAATG_Cn'
Cuts at: 2867 3259 2867
Size: 392 6488
Bsp1286I G_dGCh'C
Cuts at: 674 1176 2469 3141 3892 4503 4596 4670 4860
Size: 502 1293 672 751 611 93 74 190
Cuts at: 4860 4922 5654 674
Size: 62 732 1900
Fragments arranged by size:
1900 1293 751 732 672 611 502 190
93 74 62
BspEI T'CCGG_A
Cuts at: 6100 6100
Size: 6880
BspHI T'CATG_A
Cuts at: 2348 3075 3186 6056 2348
Size: 727 111 2870 3172
Fragments arranged by size:
3172 2870 727 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3928 4142 4444 4825 3928
Size: 214 302 381 5983
Fragments arranged by size:
5983 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3521 5170 5224 6710 6875 50
Size: 3471 1649 54 1486 165 55
Fragments arranged by size:
3471 1649 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3459 4790 3459
Size: 1331 5549
BsrFI r'CCGG_y
Cuts at: 4876 5057 5268 4876
Size: 181 211 6488
Fragments arranged by size:
6488 211 181
BssSI C'ACGA_G
Cuts at: 1918 3734 5149 5509 1918
Size: 1816 1415 360 3289
Fragments arranged by size:
3289 1816 1415 360
BstAPI GCAn_nnn'nTGC
Cuts at: 4255 4255
Size: 6880
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6880
BstEII G'GTnAC_C
Cuts at: 3224 3224
Size: 6880
BstXI CCAn_nnnn'nTGG
Cuts at: 193 2331 2821 193
Size: 2138 490 4252
Fragments arranged by size:
4252 2138 490
BstYI r'GATC_y
Cuts at: 1 829 1056 1702 1804 2031 2939 3297 3903
Size: 828 227 646 102 227 908 358 606
Cuts at: 3903 4370 4393 4728 4974 5988 6074 6086 6432
Size: 467 23 335 246 1014 86 12 346
Cuts at: 6432 1
Size: 449
Fragments arranged by size:
1014 908 828 646 606 467 449 358
346 335 246 227 227 102 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 618 618
Size: 6880
BtgI C'CryG_G
Cuts at: 1112 1112
Size: 6880
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2243 2959 4828 5009 5172 2243
Size: 716 1869 181 163 3951
Fragments arranged by size:
3951 1869 716 181 163
BtsI GCAGTG_nn'
Cuts at: 198 2353 2968 4121 198
Size: 2155 615 1153 2957
Fragments arranged by size:
2957 2155 1153 615
DraI TTT'AAA
Cuts at: 3314 3877 3314
Size: 563 6317
DrdI GACnn_nn'nnGTC
Cuts at: 4584 5444 4584
Size: 860 6020
EaeI y'GGCC_r
Cuts at: 2264 4463 4637 5028 5055 6402 2264
Size: 2199 174 391 27 1347 2742
Fragments arranged by size:
2742 2199 1347 391 174 27
EagI C'GGCC_G
Cuts at: 4463 4463
Size: 6880
EarI CTCTTCn'nnn_
Cuts at: 1256 1391 1445 1878 2591 2896 4901 5111 1256
Size: 135 54 433 713 305 2005 210 3025
Fragments arranged by size:
3025 2005 713 433 305 210 135 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5086 5398 5544 6690 5086
Size: 312 146 1146 5276
Fragments arranged by size:
5276 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3890 3890
Size: 6880
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 328 559 1367 1415 2068 2180 2711 3011
Size: 30 231 808 48 653 112 531 300
Cuts at: 3011 3038 3086 3681 3735 4703 5135 5884 6152
Size: 27 48 595 54 968 432 749 268
Cuts at: 6152 298
Size: 1026
Fragments arranged by size:
1026 968 808 749 653 595 531 432
300 268 231 112 54 48 48 30
27
EcoNI CCTnn'n_nnAGG
Cuts at: 370 1387 3717 370
Size: 1017 2330 3533
Fragments arranged by size:
3533 2330 1017
EcoO109I rG'GnC_Cy
Cuts at: 182 3767 4017 182
Size: 3585 250 3045
Fragments arranged by size:
3585 3045 250
EcoRI G'AATT_C
Cuts at: 3882 3882
Size: 6880
EcoRV GAT'ATC
Cuts at: 378 378
Size: 6880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 970 1306 2602 2677 4897 970
Size: 336 1296 75 2220 2953
Fragments arranged by size:
2953 2220 1296 336 75
FspI TGC'GCA
Cuts at: 4659 4659
Size: 6880
HaeII r_GCGC'y
Cuts at: 4312 4560 5584 6563 4312
Size: 248 1024 979 4629
Fragments arranged by size:
4629 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 322 354 367 399 860 892 1807
Size: 32 97 32 13 32 461 32 915
Cuts at: 1807 1839 2023 2055 2361 2393 2469 2501 2981
Size: 32 184 32 306 32 76 32 480
Cuts at: 2981 3013 4720 4750 4752 4782 193
Size: 32 1707 30 2 30 2291
Fragments arranged by size:
2291 1707 915 480 461 306 184 97
76 32 32 32 32 32 32 32
32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 1444 3800 3917 1444
Size: 2356 117 4407
Fragments arranged by size:
4407 2356 117
HpaI GTT'AAC
Cuts at: 3800 3800
Size: 6880
KasI G'GCGC_C
Cuts at: 4308 4556 4308
Size: 248 6632
KpnI G_GTAC'C
Cuts at: 2231 3898 2231
Size: 1667 5213
MfeI C'AATT_G
Cuts at: 1717 2092 3482 1717
Size: 375 1390 5115
Fragments arranged by size:
5115 1390 375
MluI A'CGCG_T
Cuts at: 6313 6313
Size: 6880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3061 3619 5551 5735 3061
Size: 558 1932 184 4206
Fragments arranged by size:
4206 1932 558 184
MscI TGG'CCA
Cuts at: 2266 4639 2266
Size: 2373 4507
MslI CAynn'nnrTG
Cuts at: 166 1876 1981 2057 2507 4867 4994 166
Size: 1710 105 76 450 2360 127 2052
Fragments arranged by size:
2360 2052 1710 450 127 105 76
MspA1I CmG'CkG
Cuts at: 225 1783 3787 3989 4303 4663 5678 5923 225
Size: 1558 2004 202 314 360 1015 245 1182
Fragments arranged by size:
2004 1558 1182 1015 360 314 245 202
NaeI GCC'GGC
Cuts at: 5059 5059
Size: 6880
NarI GG'CG_CC
Cuts at: 4309 4557 4309
Size: 248 6632
NcoI C'CATG_G
Cuts at: 1112 1112
Size: 6880
NgoMIV G'CCGG_C
Cuts at: 5057 5057
Size: 6880
NheI G'CTAG_C
Cuts at: 1296 2070 1296
Size: 774 6106
NruI TCG'CGA
Cuts at: 5241 5241
Size: 6880
NspI r_CATG'y
Cuts at: 3931 4431 5340 3931
Size: 500 909 5471
Fragments arranged by size:
5471 909 500
PasI CC'CwG_GG
Cuts at: 2712 3654 2712
Size: 942 5938
PciI A'CATG_T
Cuts at: 5336 5336
Size: 6880
PflMI CCAn_nnn'nTGG
Cuts at: 3006 3006
Size: 6880
PfoI T'CCnGG_A
Cuts at: 2999 2999
Size: 6880
PmeI GTTT'AAAC
Cuts at: 3877 3877
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6019 6051 6019
Size: 32 6848
PpuMI rG'GwC_Cy
Cuts at: 182 3767 182
Size: 3585 3295
PspOMI G'GGCC_C
Cuts at: 3137 3137
Size: 6880
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1739 1771 1739
Size: 32 6848
PstI C_TGCA'G
Cuts at: 466 2700 3925 4610 466
Size: 2234 1225 685 2736
Fragments arranged by size:
2736 2234 1225 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6880
PvuII CAG'CTG
Cuts at: 225 1783 3787 4303 4663 225
Size: 1558 2004 516 360 2442
Fragments arranged by size:
2442 2004 1558 516 360
RsrII CG'GwC_CG
Cuts at: 5073 5073
Size: 6880
SacI G_AGCT'C
Cuts at: 3892 3892
Size: 6880
SalI G'TCGA_C
Cuts at: 3915 3915
Size: 6880
SanDI GG'GwC_CC
Cuts at: 182 182
Size: 6880
SapI GCTCTTCn'nnn_
Cuts at: 4901 5111 4901
Size: 210 6670
SbfI CC_TGCA'GG
Cuts at: 466 3925 466
Size: 3459 3421
ScaI AGT'ACT
Cuts at: 1949 6397 1949
Size: 4448 2432
SfcI C'TryA_G
Cuts at: 33 462 905 2144 2696 3157 3564 3921 4606
Size: 429 443 1239 552 461 407 357 685
Cuts at: 4606 5601 5792 33
Size: 995 191 1121
Fragments arranged by size:
1239 1121 995 685 552 461 443 429
407 357 191
SfoI GGC'GCC
Cuts at: 4310 4558 4310
Size: 248 6632
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6880
SmaI CCC'GGG
Cuts at: 3900 3900
Size: 6880
SmlI C'TyrA_G
Cuts at: 646 1291 2536 2735 3514 4033 4948 5442 5704
Size: 645 1245 199 779 519 915 494 262
Cuts at: 5704 646
Size: 1822
Fragments arranged by size:
1822 1245 915 779 645 519 494 262
199
SnaBI TAC'GTA
Cuts at: 3870 3870
Size: 6880
SpeI A'CTAG_T
Cuts at: 67 925 67
Size: 858 6022
SphI G_CATG'C
Cuts at: 3931 4431 3931
Size: 500 6380
SspI AAT'ATT
Cuts at: 1413 1413
Size: 6880
StuI AGG'CCT
Cuts at: 682 2201 682
Size: 1519 5361
StyI C'CwwG_G
Cuts at: 683 1112 1388 1858 4012 4366 683
Size: 429 276 470 2154 354 3197
Fragments arranged by size:
3197 2154 470 429 354 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 347 1864 2237 3057 3473 3622 4461 4594 5053
Size: 1517 373 820 416 149 839 133 459
Cuts at: 5053 5264 347
Size: 211 1963
Fragments arranged by size:
1963 1517 839 820 459 416 373 211
149 133
TatI w'GTAC_w
Cuts at: 1947 3233 3741 6395 1947
Size: 1286 508 2654 2432
Fragments arranged by size:
2654 2432 1286 508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1796 1828 1906 1938 6397 6429 1796
Size: 32 78 32 4459 32 2247
Fragments arranged by size:
4459 2247 78 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4675 4675
Size: 6880
XbaI T'CTAG_A
Cuts at: 261 477 1596 1770 3909 261
Size: 216 1119 174 2139 3232
Fragments arranged by size:
3232 2139 1119 216 174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2950 4373 2950
Size: 1423 5457
XmaI C'CCGG_G
Cuts at: 3898 3898
Size: 6880
XmnI GAAnn'nnTTC
Cuts at: 473 3051 3209 473
Size: 2578 158 4144
Fragments arranged by size:
4144 2578 158
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BcgI BfrBI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI
CspCI DraIII FseI FspAI HindIII NdeI NotI NsiI
PacI PmlI PshAI PsiI PspXI SacII SexAI SfiI
SgrAI SrfI SwaI XhoI ZraI