Restriction map for
pF1KSDB0027
(Circular) (Six-base) MAPSORT of: pF1KSDB0027.seq Check: 5682 from: 1 to: 6076
pF1KSDB0027 6076 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 1547 1547
Size: 6076
AccI GT'mk_AC
Cuts at: 2216 3062 3112 2216
Size: 846 50 5180
Fragments arranged by size:
5180 846 50
Acc65I G'GTAC_C
Cuts at: 3090 3090
Size: 6076
AclI AA'CG_TT
Cuts at: 1208 5916 1208
Size: 4708 1368
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3899 4331 5080 5348 3899
Size: 432 749 268 4627
Fragments arranged by size:
4627 749 432 268
AfeI AGC'GCT
Cuts at: 363 2224 363
Size: 1861 4215
AflIII A'CryG_T
Cuts at: 2732 2945 3426 4532 5509 2732
Size: 213 481 1106 977 3299
Fragments arranged by size:
3299 1106 977 481 213
AgeI A'CCGG_T
Cuts at: 4464 4464
Size: 6076
AhdI GACnn_n'nnGTC
Cuts at: 462 462
Size: 6076
AleI CACnn'nnGTG
Cuts at: 2236 2236
Size: 6076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1073 1666 1073
Size: 593 5483
AlwNI CAG_nnn'CTG
Cuts at: 429 441 1020 1534 1731 2085 3379 4948 429
Size: 12 579 514 197 354 1294 1569 1557
Fragments arranged by size:
1569 1557 1294 579 514 354 197 12
ApaI G_GGCC'C
Cuts at: 144 1014 144
Size: 870 5206
ApaLI G'TGCA_C
Cuts at: 2784 4846 2784
Size: 2062 4014
ApoI r'AATT_y
Cuts at: 1512 3078 5429 5685 1512
Size: 1566 2351 256 1903
Fragments arranged by size:
2351 1903 1566 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6076
AvaI C'yCGr_G
Cuts at: 192 775 1081 1618 2865 3094 192
Size: 583 306 537 1247 229 3174
Fragments arranged by size:
3174 1247 583 537 306 229
BamHI G'GATC_C
Cuts at: 131 3099 131
Size: 2968 3108
BanI G'GyrC_C
Cuts at: 1657 1689 1938 2029 2533 2751 3090 3504 3752
Size: 32 249 91 504 218 339 414 248
Cuts at: 3752 3787 1657
Size: 35 3946
Fragments arranged by size:
3946 504 414 339 249 248 218 91
35 32
BanII G_rGCy'C
Cuts at: 144 199 327 350 849 1014 2120 2213 2452
Size: 55 128 23 499 165 1106 93 239
Cuts at: 2452 2926 3088 4118 144
Size: 474 162 1030 2102
Fragments arranged by size:
2102 1106 1030 499 474 239 165 162
128 93 55 23
BbeI G_GCGC'C
Cuts at: 1661 3508 3756 1661
Size: 1847 248 3981
Fragments arranged by size:
3981 1847 248
BbsI GAAGACnn'nnnn_
Cuts at: 872 1551 2135 2251 872
Size: 679 584 116 4697
Fragments arranged by size:
4697 679 584 116
BbvCI CC'TCA_GC
Cuts at: 1667 1948 1667
Size: 281 5795
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2835 2869 2835
Size: 34 6042
BciVI GTATCCnnnnn_n'
Cuts at: 3972 4735 6076 3972
Size: 763 1341 3972
Fragments arranged by size:
3972 1341 763
BclI T'GATC_A
Cuts at: 3594 3594
Size: 6076
BglI GCCn_nnn'nGGC
Cuts at: 313 322 1219 2581 2726 313
Size: 9 897 1362 145 3663
Fragments arranged by size:
3663 1362 897 145 9
BglII A'GATC_T
Cuts at: 857 3589 5628 857
Size: 2732 2039 1305
Fragments arranged by size:
2732 2039 1305
BlpI GC'TnA_GC
Cuts at: 1448 1527 1777 2335 3186 1448
Size: 79 250 558 851 4338
Fragments arranged by size:
4338 851 558 250 79
Bme1580I G_kGCm'C
Cuts at: 144 1014 1110 1694 1943 2536 2740 2788 3699
Size: 870 96 584 249 593 204 48 911
Cuts at: 3699 3792 4850 144
Size: 93 1058 1370
Fragments arranged by size:
1370 1058 911 870 593 584 249 204
96 93 48
BmgBI CAC'GTC
Cuts at: 2789 2789
Size: 6076
BmrI ACTGGGnnnn_n'
Cuts at: 1803 3286 3367 3702 5848 1803
Size: 1483 81 335 2146 2031
Fragments arranged by size:
2146 2031 1483 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2574 2841 4441 2574
Size: 267 1600 4209
Fragments arranged by size:
4209 1600 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1933 2173 1933
Size: 240 5836
Bpu10I CC'TnA_GC
Cuts at: 1667 1703 1948 2255 2282 1667
Size: 36 245 307 27 5461
Fragments arranged by size:
5461 307 245 36 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 595 1237 1333 1810 2104 3016 3250 4129
Size: 45 642 96 477 294 912 234 879
Cuts at: 4129 4623 4921 550
Size: 494 298 1705
Fragments arranged by size:
1705 912 879 642 494 477 298 294
234 96 45
BsaI GGTCTCn'nnnn_
Cuts at: 341 348 2462 5780 341
Size: 7 2114 3318 637
Fragments arranged by size:
3318 2114 637 7
BsaAI yAC'GTr
Cuts at: 2367 2733 3066 3427 2367
Size: 366 333 361 5016
Fragments arranged by size:
5016 366 361 333
BsaBI GATnn'nnATC
Cuts at: 6 1300 3588 5627 6
Size: 1294 2288 2039 455
Fragments arranged by size:
2288 2039 1294 455
BsaHI Gr'CG_yC
Cuts at: 893 1544 1658 2021 3505 3753 4457 5953 893
Size: 651 114 363 1484 248 704 1496 1016
Fragments arranged by size:
1496 1484 1016 704 651 363 248 114
BsaWI w'CCGG_w
Cuts at: 965 3263 3784 4464 4738 4885 5296 965
Size: 2298 521 680 274 147 411 1745
Fragments arranged by size:
2298 1745 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2146 2176 2146
Size: 30 6046
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 693 1378 1597 2830 693
Size: 685 219 1233 3939
Fragments arranged by size:
3939 1233 685 219
BseYI C'CCAG_C
Cuts at: 358 788 1653 2273 3499 4257 4836 5332 358
Size: 430 865 620 1226 758 579 496 1102
Fragments arranged by size:
1226 1102 865 758 620 579 496 430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1520 1911 2260 2653 2899 514
Size: 1006 391 349 393 246 3691
Fragments arranged by size:
3691 1006 393 391 349 246
BsiEI CG_ry'CG
Cuts at: 104 213 2721 3616 3662 4872 104
Size: 109 2508 895 46 1210 1308
Fragments arranged by size:
2508 1308 1210 895 109 46
BsiHKAI G_wGCw'C
Cuts at: 1052 1528 2120 2452 2788 3088 3866 4056 4850
Size: 476 592 332 336 300 778 190 794
Cuts at: 4850 1052
Size: 2278
Fragments arranged by size:
2278 794 778 592 476 336 332 300
190
BsmI GAATG_Cn'
Cuts at: 728 728
Size: 6076
Bsp1286I G_dGCh'C
Cuts at: 144 199 327 350 849 1014 1052 1110 1528
Size: 55 128 23 499 165 38 58 418
Cuts at: 1528 1694 1943 2120 2213 2452 2536 2740 2788
Size: 166 249 177 93 239 84 204 48
Cuts at: 2788 2926 3088 3699 3792 3866 4056 4118 4850
Size: 138 162 611 93 74 190 62 732
Cuts at: 4850 144
Size: 1370
Fragments arranged by size:
1370 732 611 499 418 249 239 204
190 177 166 165 162 138 128 93
93 84 74 62 58 55 48 38
23
BspEI T'CCGG_A
Cuts at: 5296 5296
Size: 6076
BspHI T'CATG_A
Cuts at: 917 2312 2396 5252 917
Size: 1395 84 2856 1741
Fragments arranged by size:
2856 1741 1395 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 426 809 1706 2012 3124 3338 3640 4021 426
Size: 383 897 306 1112 214 302 381 2481
Fragments arranged by size:
2481 1112 897 383 381 306 302 214
BsrBI CCG'CTC
Cuts at: 50 1452 1757 4366 4420 5906 6071 50
Size: 1402 305 2609 54 1486 165 55
Fragments arranged by size:
2609 1486 1402 305 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 2401 3986 1661
Size: 740 1585 3751
Fragments arranged by size:
3751 1585 740
BsrFI r'CCGG_y
Cuts at: 4072 4253 4464 4072
Size: 181 211 5684
Fragments arranged by size:
5684 211 181
BsrGI T'GTAC_A
Cuts at: 2267 2267
Size: 6076
BssSI C'ACGA_G
Cuts at: 1441 4345 4705 1441
Size: 2904 360 2812
Fragments arranged by size:
2904 2812 360
BstAPI GCAn_nnn'nTGC
Cuts at: 429 441 3451 429
Size: 12 3010 3054
Fragments arranged by size:
3054 3010 12
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6076
BstYI r'GATC_y
Cuts at: 1 131 857 962 2328 3099 3566 3589 3924
Size: 130 726 105 1366 771 467 23 335
Cuts at: 3924 4170 5184 5270 5282 5628 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1366 1014 771 726 467 449 346 335
246 130 105 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 318 2435 2794 318
Size: 2117 359 3600
Fragments arranged by size:
3600 2117 359
BtgI C'CryG_G
Cuts at: 311 401 2835 311
Size: 90 2434 3552
Fragments arranged by size:
3552 2434 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 710 729 963 1746 2915 4024 4205 4368 710
Size: 19 234 783 1169 1109 181 163 2418
Fragments arranged by size:
2418 1169 1109 783 234 181 163 19
BtsI GCAGTG_nn'
Cuts at: 948 1432 1902 2689 3317 948
Size: 484 470 787 628 3707
Fragments arranged by size:
3707 787 628 484 470
DraI TTT'AAA
Cuts at: 3073 3073
Size: 6076
DraIII CAC_nnn'GTG
Cuts at: 1797 1797
Size: 6076
DrdI GACnn_nn'nnGTC
Cuts at: 1969 3780 4640 1969
Size: 1811 860 3405
Fragments arranged by size:
3405 1811 860
EaeI y'GGCC_r
Cuts at: 210 305 1244 2036 2171 2345 2718 3659 3833
Size: 95 939 792 135 174 373 941 174
Cuts at: 3833 4224 4251 5598 210
Size: 391 27 1347 688
Fragments arranged by size:
1347 941 939 792 688 391 373 174
174 135 95 27
EagI C'GGCC_G
Cuts at: 210 2718 3659 210
Size: 2508 941 2627
Fragments arranged by size:
2627 2508 941
EarI CTCTTCn'nnn_
Cuts at: 1615 1913 2146 4097 4307 1615
Size: 298 233 1951 210 3384
Fragments arranged by size:
3384 1951 298 233 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 124 260 268 4282 4594 4740 5886 124
Size: 136 8 4014 312 146 1146 314
Fragments arranged by size:
4014 1146 314 312 146 136 8
EcoICRI GAG'CTC
Cuts at: 2118 2450 3086 2118
Size: 332 636 5108
Fragments arranged by size:
5108 636 332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1933 2173 3899 4331 5080 5348 1933
Size: 240 1726 432 749 268 2661
Fragments arranged by size:
2661 1726 749 432 268 240
EcoNI CCTnn'n_nnAGG
Cuts at: 433 1348 2347 433
Size: 915 999 4162
Fragments arranged by size:
4162 999 915
EcoO109I rG'GnC_Cy
Cuts at: 141 235 426 438 905 1187 2136 2352 3213
Size: 94 191 12 467 282 949 216 861
Cuts at: 3213 141
Size: 3004
Fragments arranged by size:
3004 949 861 467 282 216 191 94
12
EcoRI G'AATT_C
Cuts at: 3078 3078
Size: 6076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1425 1764 2157 4093 1425
Size: 339 393 1936 3408
Fragments arranged by size:
3408 1936 393 339
FspI TGC'GCA
Cuts at: 3855 3855
Size: 6076
HaeII r_GCGC'y
Cuts at: 365 1021 1661 2226 3508 3756 4780 5759 365
Size: 656 640 565 1282 248 1024 979 682
Fragments arranged by size:
1282 1024 979 682 656 640 565 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 688 720 1486 1518 2146 2178 2238
Size: 32 208 32 766 32 628 32 60
Cuts at: 2238 2270 3916 3946 3948 3978 448
Size: 32 1646 30 2 30 2546
Fragments arranged by size:
2546 1646 766 628 208 60 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2862 3113 2862
Size: 251 5825
KasI G'GCGC_C
Cuts at: 1657 3504 3752 1657
Size: 1847 248 3981
Fragments arranged by size:
3981 1847 248
KpnI G_GTAC'C
Cuts at: 3094 3094
Size: 6076
MluI A'CGCG_T
Cuts at: 5509 5509
Size: 6076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1529 1715 4747 4931 1529
Size: 186 3032 184 2674
Fragments arranged by size:
3032 2674 186 184
MscI TGG'CCA
Cuts at: 2173 3835 2173
Size: 1662 4414
MslI CAynn'nnrTG
Cuts at: 1306 1648 2236 2605 2845 4063 4190 1306
Size: 342 588 369 240 1218 127 3192
Fragments arranged by size:
3192 1218 588 369 342 240 127
MspA1I CmG'CkG
Cuts at: 227 313 447 1531 2339 2376 2837 3027 3185
Size: 86 134 1084 808 37 461 190 158
Cuts at: 3185 3499 3859 4874 5119 227
Size: 314 360 1015 245 1184
Fragments arranged by size:
1184 1084 1015 808 461 360 314 245
190 158 134 86 37
NaeI GCC'GGC
Cuts at: 4255 4255
Size: 6076
NarI GG'CG_CC
Cuts at: 1658 3505 3753 1658
Size: 1847 248 3981
Fragments arranged by size:
3981 1847 248
NcoI C'CATG_G
Cuts at: 401 401
Size: 6076
NgoMIV G'CCGG_C
Cuts at: 4253 4253
Size: 6076
NruI TCG'CGA
Cuts at: 4437 4437
Size: 6076
NspI r_CATG'y
Cuts at: 1158 1179 1680 1821 2421 2604 2949 3127 3627
Size: 21 501 141 600 183 345 178 500
Cuts at: 3627 4536 1158
Size: 909 2698
Fragments arranged by size:
2698 909 600 501 500 345 183 178
141 21
PciI A'CATG_T
Cuts at: 2945 4532 2945
Size: 1587 4489
PflMI CCAn_nnn'nTGG
Cuts at: 407 1797 2748 407
Size: 1390 951 3735
Fragments arranged by size:
3735 1390 951
PfoI T'CCnGG_A
Cuts at: 1562 1859 1958 1562
Size: 297 99 5680
Fragments arranged by size:
5680 297 99
PmeI GTTT'AAAC
Cuts at: 3073 3073
Size: 6076
PmlI CAC'GTG
Cuts at: 2367 2367
Size: 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5215 5247 5215
Size: 32 6044
PpuMI rG'GwC_Cy
Cuts at: 235 438 1187 2136 235
Size: 203 749 949 4175
Fragments arranged by size:
4175 949 749 203
PshAI GACnn'nnGTC
Cuts at: 567 567
Size: 6076
PsiI TTA'TAA
Cuts at: 2954 2954
Size: 6076
PspOMI G'GGCC_C
Cuts at: 140 1010 140
Size: 870 5206
PstI C_TGCA'G
Cuts at: 434 446 767 854 938 955 1184 1897 2186
Size: 12 321 87 84 17 229 713 289
Cuts at: 2186 2765 3121 3806 434
Size: 579 356 685 2704
Fragments arranged by size:
2704 713 685 579 356 321 289 229
87 84 17 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6076
PvuII CAG'CTG
Cuts at: 227 447 2376 3499 3859 227
Size: 220 1929 1123 360 2444
Fragments arranged by size:
2444 1929 1123 360 220
RsrII CG'GwC_CG
Cuts at: 4269 4269
Size: 6076
SacI G_AGCT'C
Cuts at: 2120 2452 3088 2120
Size: 332 636 5108
Fragments arranged by size:
5108 636 332
SacII CC_GC'GG
Cuts at: 314 2838 314
Size: 2524 3552
SalI G'TCGA_C
Cuts at: 3111 3111
Size: 6076
SanDI GG'GwC_CC
Cuts at: 235 235
Size: 6076
SapI GCTCTTCn'nnn_
Cuts at: 4097 4307 4097
Size: 210 5866
SbfI CC_TGCA'GG
Cuts at: 3121 3121
Size: 6076
ScaI AGT'ACT
Cuts at: 1324 5593 1324
Size: 4269 1807
SexAI A'CCwGG_T
Cuts at: 2651 2651
Size: 6076
SfcI C'TryA_G
Cuts at: 33 430 442 763 850 934 951 1180 1893
Size: 397 12 321 87 84 17 229 713
Cuts at: 1893 2045 2182 2761 2906 3117 3802 4797 4988
Size: 152 137 579 145 211 685 995 191
Cuts at: 4988 33
Size: 1121
Fragments arranged by size:
1121 995 713 685 579 397 321 229
211 191 152 145 137 87 84 17
12
SfiI GGCCn_nnn'nGGCC
Cuts at: 313 313
Size: 6076
SfoI GGC'GCC
Cuts at: 1659 3506 3754 1659
Size: 1847 248 3981
Fragments arranged by size:
3981 1847 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6076
SmaI CCC'GGG
Cuts at: 2867 3096 2867
Size: 229 5847
SmlI C'TyrA_G
Cuts at: 565 610 1216 1348 1825 2119 3031 3229 4144
Size: 45 606 132 477 294 912 198 915
Cuts at: 4144 4638 4900 565
Size: 494 262 1741
Fragments arranged by size:
1741 915 912 606 494 477 294 262
198 132 45
SnaBI TAC'GTA
Cuts at: 3066 3066
Size: 6076
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6076
SphI G_CATG'C
Cuts at: 1680 2421 2604 3127 3627 1680
Size: 741 183 523 500 4129
Fragments arranged by size:
4129 741 523 500 183
SspI AAT'ATT
Cuts at: 1032 1032
Size: 6076
StuI AGG'CCT
Cuts at: 267 1222 1604 1747 1924 267
Size: 955 382 143 177 4419
Fragments arranged by size:
4419 955 382 177 143
StyI C'CwwG_G
Cuts at: 401 752 1748 2779 3208 3562 401
Size: 351 996 1031 429 354 2915
Fragments arranged by size:
2915 1031 996 429 354 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1614 1785 2287 2347 2697 2769 2907 3657 3790
Size: 171 502 60 350 72 138 750 133
Cuts at: 3790 4249 4460 1614
Size: 459 211 3230
Fragments arranged by size:
3230 750 502 459 350 211 171 138
133 72 60
TatI w'GTAC_w
Cuts at: 1172 1322 2267 5591 1172
Size: 150 945 3324 1657
Fragments arranged by size:
3324 1657 945 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1529 1561 5593 5625 1529
Size: 32 4032 32 1980
Fragments arranged by size:
4032 1980 32 32
Tth111I GACn'n_nGTC
Cuts at: 3871 3871
Size: 6076
XbaI T'CTAG_A
Cuts at: 3105 3105
Size: 6076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2031 2691 2721 3569 2031
Size: 660 30 848 4538
Fragments arranged by size:
4538 848 660 30
XmaI C'CCGG_G
Cuts at: 2865 3094 2865
Size: 229 5847
XmnI GAAnn'nnTTC
Cuts at: 994 994
Size: 6076
ZraI GAC'GTC
Cuts at: 1545 1545
Size: 6076
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflII AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PspXI PsrI SgrAI SrfI
SwaI XhoI