Restriction map for
pF1KSDB0030
(Circular) (Six-base) MAPSORT of: pF1KSDB0030.seq Check: 2244 from: 1 to: 7957
pF1KSDB0030 7957 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3693 3693
Size: 7957
AatII G_ACGT'C
Cuts at: 3061 3061
Size: 7957
AccI GT'mk_AC
Cuts at: 386 4000 4078 4417 4993 386
Size: 3614 78 339 576 3350
Fragments arranged by size:
3614 3350 576 339 78
Acc65I G'GTAC_C
Cuts at: 3534 4971 3534
Size: 1437 6520
AclI AA'CG_TT
Cuts at: 1977 7797 1977
Size: 5820 2137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 1268 1469 2806 2890 3687 3865 4391 4519
Size: 375 201 1337 84 797 178 526 128
Cuts at: 4519 5780 6212 6961 7229 893
Size: 1261 432 749 268 1621
Fragments arranged by size:
1621 1337 1261 797 749 526 432 375
268 201 178 128 84
AflII C'TTAA_G
Cuts at: 1389 2917 1389
Size: 1528 6429
AflIII A'CryG_T
Cuts at: 2597 3561 4151 4574 5307 6413 7390 2597
Size: 964 590 423 733 1106 977 3164
Fragments arranged by size:
3164 1106 977 964 733 590 423
AgeI A'CCGG_T
Cuts at: 848 1146 6345 848
Size: 298 5199 2460
Fragments arranged by size:
5199 2460 298
AleI CACnn'nnGTG
Cuts at: 616 1663 616
Size: 1047 6910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1849 3133 3954 1849
Size: 1284 821 5852
Fragments arranged by size:
5852 1284 821
AlwNI CAG_nnn'CTG
Cuts at: 358 1159 1675 1819 2481 2653 3481 3951 4047
Size: 801 516 144 662 172 828 470 96
Cuts at: 4047 5260 6829 358
Size: 1213 1569 1486
Fragments arranged by size:
1569 1486 1213 828 801 662 516 470
172 144 96
ApaI G_GGCC'C
Cuts at: 315 747 2195 315
Size: 432 1448 6077
Fragments arranged by size:
6077 1448 432
ApaLI G'TGCA_C
Cuts at: 6727 6727
Size: 7957
ApoI r'AATT_y
Cuts at: 556 689 1217 1638 1886 4360 4959 7310 7566
Size: 133 528 421 248 2474 599 2351 256
Cuts at: 7566 556
Size: 947
Fragments arranged by size:
2474 2351 947 599 528 421 256 248
133
AseI AT'TA_AT
Cuts at: 20 20
Size: 7957
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7957
AvaI C'yCGr_G
Cuts at: 198 321 338 1912 2807 4975 198
Size: 123 17 1574 895 2168 3180
Fragments arranged by size:
3180 2168 1574 895 123 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 487 520 487
Size: 33 7924
BamHI G'GATC_C
Cuts at: 397 2103 4980 397
Size: 1706 2877 3374
Fragments arranged by size:
3374 2877 1706
BanI G'GyrC_C
Cuts at: 574 1443 3117 3534 4203 4971 5385 5633 5668
Size: 869 1674 417 669 768 414 248 35
Cuts at: 5668 574
Size: 2863
Fragments arranged by size:
2863 1674 869 768 669 417 414 248
35
BanII G_rGCy'C
Cuts at: 315 525 747 1368 2195 2573 3841 4590 4969
Size: 210 222 621 827 378 1268 749 379
Cuts at: 4969 5999 315
Size: 1030 2273
Fragments arranged by size:
2273 1268 1030 827 749 621 379 378
222 210
BbeI G_GCGC'C
Cuts at: 578 1447 5389 5637 578
Size: 869 3942 248 2898
Fragments arranged by size:
3942 2898 869 248
BbsI GAAGACnn'nnnn_
Cuts at: 1886 3061 1886
Size: 1175 6782
BbvCI CC'TCA_GC
Cuts at: 4124 4124
Size: 7957
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4659 4693 4659
Size: 34 7923
BciVI GTATCCnnnnn_n'
Cuts at: 4578 5853 6616 7957 4578
Size: 1275 763 1341 4578
Fragments arranged by size:
4578 1341 1275 763
BclI T'GATC_A
Cuts at: 5475 5475
Size: 7957
BfrBI ATG'CAT
Cuts at: 2895 2895
Size: 7957
BglI GCCn_nnn'nGGC
Cuts at: 2229 3753 4732 2229
Size: 1524 979 5454
Fragments arranged by size:
5454 1524 979
BglII A'GATC_T
Cuts at: 425 1251 5470 7509 425
Size: 826 4219 2039 873
Fragments arranged by size:
4219 2039 873 826
BlpI GC'TnA_GC
Cuts at: 4348 4856 5067 4348
Size: 508 211 7238
Fragments arranged by size:
7238 508 211
Bme1580I G_kGCm'C
Cuts at: 315 747 1325 1838 2195 2630 4208 4458 5580
Size: 432 578 513 357 435 1578 250 1122
Cuts at: 5580 5673 6731 315
Size: 93 1058 1541
Fragments arranged by size:
1578 1541 1122 1058 578 513 435 432
357 250 93
BmgBI CAC'GTC
Cuts at: 1843 2061 3090 3562 4806 1843
Size: 218 1029 472 1244 4994
Fragments arranged by size:
4994 1244 1029 472 218
BmrI ACTGGGnnnn_n'
Cuts at: 625 949 1076 1390 3826 5167 5248 5583 7729
Size: 324 127 314 2436 1341 81 335 2146
Cuts at: 7729 625
Size: 853
Fragments arranged by size:
2436 2146 1341 853 335 324 314 127
81
BmtI G_CTAG'C
Cuts at: 3773 3773
Size: 7957
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3972 6322 3972
Size: 2350 5607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1073 1165 1454 3236 3428 3629 4027 4297
Size: 262 92 289 1782 192 201 398 270
Cuts at: 4297 4507 4714 811
Size: 210 207 4054
Fragments arranged by size:
4054 1782 398 289 270 262 210 207
201 192 92
Bpu10I CC'TnA_GC
Cuts at: 1502 2179 3447 4124 1502
Size: 677 1268 677 5335
Fragments arranged by size:
5335 1268 677 677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 782 1646 2962 3603 5131 6010 6504 6802 782
Size: 864 1316 641 1528 879 494 298 1937
Fragments arranged by size:
1937 1528 1316 879 864 641 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 290 1724 1855 3605 3614 4239 7661 290
Size: 1434 131 1750 9 625 3422 586
Fragments arranged by size:
3422 1750 1434 625 586 131 9
BsaAI yAC'GTr
Cuts at: 4152 4947 5308 4152
Size: 795 361 6801
Fragments arranged by size:
6801 795 361
BsaBI GATnn'nnATC
Cuts at: 6 2381 5469 7508 6
Size: 2375 3088 2039 455
Fragments arranged by size:
3088 2375 2039 455
BsaHI Gr'CG_yC
Cuts at: 575 814 1444 2781 3058 5386 5634 6338 7834
Size: 239 630 1337 277 2328 248 704 1496
Cuts at: 7834 575
Size: 698
Fragments arranged by size:
2328 1496 1337 704 698 630 277 248
239
BsaWI w'CCGG_w
Cuts at: 848 1146 5144 5665 6345 6619 6766 7177 848
Size: 298 3998 521 680 274 147 411 1628
Fragments arranged by size:
3998 1628 680 521 411 298 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 424 454 721 751 424
Size: 30 267 30 7630
Fragments arranged by size:
7630 267 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 742 1091 1280 1426 1522 1598 1701 1981 3588
Size: 349 189 146 96 76 103 280 1607
Cuts at: 3588 3632 3733 3877 3880 4444 4483 4939 4942
Size: 44 101 144 3 564 39 456 3
Cuts at: 4942 742
Size: 3757
Fragments arranged by size:
3757 1607 564 456 349 280 189 146
144 103 101 96 76 44 39 3
3
BseYI C'CCAG_C
Cuts at: 314 372 435 917 1296 1497 2353 3422 3489
Size: 58 63 482 379 201 856 1069 67
Cuts at: 3489 3743 4066 5380 6138 6717 7213 314
Size: 254 323 1314 758 579 496 1058
Fragments arranged by size:
1314 1069 1058 856 758 579 496 482
379 323 254 201 67 63 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 364 371 532 619 3226 3589 3964 4033
Size: 14 7 161 87 2607 363 375 69
Cuts at: 4033 350
Size: 4274
Fragments arranged by size:
4274 2607 375 363 161 87 69 14
7
BsiEI CG_ry'CG
Cuts at: 104 1986 5497 5543 6753 104
Size: 1882 3511 46 1210 1308
Fragments arranged by size:
3511 1882 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 3197 4969 5747 5937 6731 3197
Size: 1772 778 190 794 4423
Fragments arranged by size:
4423 1772 794 778 190
BsmI GAATG_Cn'
Cuts at: 4390 4390
Size: 7957
BsmBI CGTCTCn'nnnn_
Cuts at: 805 1683 805
Size: 878 7079
Bsp1286I G_dGCh'C
Cuts at: 315 525 747 1325 1368 1838 2195 2573 2630
Size: 210 222 578 43 470 357 378 57
Cuts at: 2630 3197 3841 4208 4458 4590 4969 5580 5673
Size: 567 644 367 250 132 379 611 93
Cuts at: 5673 5747 5937 5999 6731 315
Size: 74 190 62 732 1541
Fragments arranged by size:
1541 732 644 611 578 567 470 379
378 367 357 250 222 210 190 132
93 74 62 57 43
BspEI T'CCGG_A
Cuts at: 7177 7177
Size: 7957
BspHI T'CATG_A
Cuts at: 1221 4592 7133 1221
Size: 3371 2541 2045
Fragments arranged by size:
3371 2541 2045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3693 4775 5005 5219 5521 5902 3693
Size: 1082 230 214 302 381 5748
Fragments arranged by size:
5748 1082 381 302 230 214
BsrBI CCG'CTC
Cuts at: 50 3633 3723 6247 6301 7787 7952 50
Size: 3583 90 2524 54 1486 165 55
Fragments arranged by size:
3583 2524 1486 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 866 1325 2291 4019 5867 866
Size: 459 966 1728 1848 2956
Fragments arranged by size:
2956 1848 1728 966 459
BsrFI r'CCGG_y
Cuts at: 213 848 875 1146 2232 3662 5953 6134 6345
Size: 635 27 271 1086 1430 2291 181 211
Cuts at: 6345 213
Size: 1825
Fragments arranged by size:
2291 1825 1430 1086 635 271 211 181
27
BssSI C'ACGA_G
Cuts at: 4025 6226 6586 4025
Size: 2201 360 5396
Fragments arranged by size:
5396 2201 360
BstAPI GCAn_nnn'nTGC
Cuts at: 358 868 3090 4047 4056 5332 358
Size: 510 2222 957 9 1276 2983
Fragments arranged by size:
2983 2222 1276 957 510 9
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7957
BstXI CCAn_nnnn'nTGG
Cuts at: 618 1197 1665 4384 618
Size: 579 468 2719 4191
Fragments arranged by size:
4191 2719 579 468
BstYI r'GATC_y
Cuts at: 1 397 425 696 1251 2103 3096 3158 4280
Size: 396 28 271 555 852 993 62 1122
Cuts at: 4280 4980 5447 5470 5805 6051 7065 7151 7163
Size: 700 467 23 335 246 1014 86 12
Cuts at: 7163 7509 1
Size: 346 449
Fragments arranged by size:
1122 1014 993 852 700 555 467 449
396 346 335 271 246 86 62 28
23 12
BstZ17I GTA'TAC
Cuts at: 4418 4418
Size: 7957
Bsu36I CC'TnA_GG
Cuts at: 1346 1847 3749 1346
Size: 501 1902 5554
Fragments arranged by size:
5554 1902 501
BtgI C'CryG_G
Cuts at: 2187 2226 3270 3654 3792 4208 2187
Size: 39 1044 384 138 416 5936
Fragments arranged by size:
5936 1044 416 384 138 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5905 6086 6249 5905
Size: 181 163 7613
Fragments arranged by size:
7613 181 163
BtsI GCAGTG_nn'
Cuts at: 333 749 1833 2089 3234 4020 4658 5198 333
Size: 416 1084 256 1145 786 638 540 3092
Fragments arranged by size:
3092 1145 1084 786 638 540 416 256
ClaI AT'CG_AT
Cuts at: 2682 3380 2682
Size: 698 7259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 468 503 468
Size: 35 7922
DraI TTT'AAA
Cuts at: 4954 4954
Size: 7957
DraIII CAC_nnn'GTG
Cuts at: 1212 1212
Size: 7957
DrdI GACnn_nn'nnGTC
Cuts at: 2905 5661 6521 2905
Size: 2756 860 4341
Fragments arranged by size:
4341 2756 860
EaeI y'GGCC_r
Cuts at: 838 877 2095 2166 2504 3218 4446 5540 5714
Size: 39 1218 71 338 714 1228 1094 174
Cuts at: 5714 6105 6132 7479 838
Size: 391 27 1347 1316
Fragments arranged by size:
1347 1316 1228 1218 1094 714 391 338
174 71 39 27
EagI C'GGCC_G
Cuts at: 5540 5540
Size: 7957
EarI CTCTTCn'nnn_
Cuts at: 1526 2786 2795 2927 3976 5978 6188 1526
Size: 1260 9 132 1049 2002 210 3295
Fragments arranged by size:
3295 2002 1260 1049 210 132 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6163 6475 6621 7767 6163
Size: 312 146 1146 6353
Fragments arranged by size:
6353 1146 312 146
EcoICRI GAG'CTC
Cuts at: 4967 4967
Size: 7957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 893 1073 1165 1268 1454 1469 2806 2890
Size: 82 180 92 103 186 15 1337 84
Cuts at: 2890 3236 3428 3629 3687 3865 4027 4297 4391
Size: 346 192 201 58 178 162 270 94
Cuts at: 4391 4507 4519 4714 5780 6212 6961 7229 811
Size: 116 12 195 1066 432 749 268 1539
Fragments arranged by size:
1539 1337 1066 749 432 346 270 268
201 195 192 186 180 178 162 116
103 94 92 84 82 58 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1793 3778 4426 1793
Size: 1985 648 5324
Fragments arranged by size:
5324 1985 648
EcoO109I rG'GnC_Cy
Cuts at: 151 449 563 743 2192 2245 2266 2421 3139
Size: 298 114 180 1449 53 21 155 718
Cuts at: 3139 3256 4309 4734 4848 5094 151
Size: 117 1053 425 114 246 3014
Fragments arranged by size:
3014 1449 1053 718 425 298 246 180
155 117 114 114 53 21
EcoRI G'AATT_C
Cuts at: 689 4959 689
Size: 4270 3687
EcoRV GAT'ATC
Cuts at: 720 720
Size: 7957
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1153 2956 3717 4363 5974 1153
Size: 1803 761 646 1611 3136
Fragments arranged by size:
3136 1803 1611 761 646
FspI TGC'GCA
Cuts at: 3760 5736 3760
Size: 1976 5981
HaeII r_GCGC'y
Cuts at: 214 578 1447 2256 2620 2829 4720 5389 5637
Size: 364 869 809 364 209 1891 669 248
Cuts at: 5637 6661 7640 214
Size: 1024 979 531
Fragments arranged by size:
1891 1024 979 869 809 669 531 364
364 248 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 721 753 835 867 1255 1287 2920 2952 3182
Size: 32 82 32 388 32 1633 32 230
Cuts at: 3182 3214 3457 3489 3865 3897 4915 4947 5797
Size: 32 243 32 376 32 1018 32 850
Cuts at: 5797 5827 5829 5859 721
Size: 30 2 30 2819
Fragments arranged by size:
2819 1633 1018 850 388 376 243 230
82 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2879 4994 2879
Size: 2115 5842
HpaI GTT'AAC
Cuts at: 2879 2879
Size: 7957
KasI G'GCGC_C
Cuts at: 574 1443 5385 5633 574
Size: 869 3942 248 2898
Fragments arranged by size:
3942 2898 869 248
KpnI G_GTAC'C
Cuts at: 3538 4975 3538
Size: 1437 6520
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 7957
MluI A'CGCG_T
Cuts at: 7390 7390
Size: 7957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 961 1357 1707 4415 4595 6628 6812 961
Size: 396 350 2708 180 2033 184 2106
Fragments arranged by size:
2708 2106 2033 396 350 184 180
MscI TGG'CCA
Cuts at: 2506 3220 4448 5716 2506
Size: 714 1228 1268 4747
Fragments arranged by size:
4747 1268 1228 714
MslI CAynn'nnrTG
Cuts at: 616 1663 2054 2900 3202 3914 5944 6071 616
Size: 1047 391 846 302 712 2030 127 2502
Fragments arranged by size:
2502 2030 1047 846 712 391 302 127
MspA1I CmG'CkG
Cuts at: 376 1381 2481 2502 2606 3440 4044 4056 4485
Size: 1005 1100 21 104 834 604 12 429
Cuts at: 4485 4521 4855 5066 5380 5740 6755 7000 376
Size: 36 334 211 314 360 1015 245 1333
Fragments arranged by size:
1333 1100 1015 1005 834 604 429 360
334 314 245 211 104 36 21 12
NaeI GCC'GGC
Cuts at: 2234 6136 2234
Size: 3902 4055
NarI GG'CG_CC
Cuts at: 575 1444 5386 5634 575
Size: 869 3942 248 2898
Fragments arranged by size:
3942 2898 869 248
NcoI C'CATG_G
Cuts at: 4208 4208
Size: 7957
NdeI CA'TA_TG
Cuts at: 4034 4034
Size: 7957
NgoMIV G'CCGG_C
Cuts at: 2232 6134 2232
Size: 3902 4055
NheI G'CTAG_C
Cuts at: 3769 3769
Size: 7957
NruI TCG'CGA
Cuts at: 6318 6318
Size: 7957
NsiI A_TGCA'T
Cuts at: 2897 2897
Size: 7957
NspI r_CATG'y
Cuts at: 2601 5008 5508 6417 2601
Size: 2407 500 909 4141
Fragments arranged by size:
4141 2407 909 500
PasI CC'CwG_GG
Cuts at: 444 4422 444
Size: 3978 3979
PciI A'CATG_T
Cuts at: 2597 6413 2597
Size: 3816 4141
PflMI CCAn_nnn'nTGG
Cuts at: 2203 2203
Size: 7957
PmeI GTTT'AAAC
Cuts at: 4954 4954
Size: 7957
PmlI CAC'GTG
Cuts at: 4152 4152
Size: 7957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7096 7128 7096
Size: 32 7925
PpuMI rG'GwC_Cy
Cuts at: 563 2245 2266 2421 3139 4309 4848 563
Size: 1682 21 155 718 1170 539 3672
Fragments arranged by size:
3672 1682 1170 718 539 155 21
PshAI GACnn'nnGTC
Cuts at: 3236 4600 3236
Size: 1364 6593
PspOMI G'GGCC_C
Cuts at: 311 743 2191 311
Size: 432 1448 6077
Fragments arranged by size:
6077 1448 432
PstI C_TGCA'G
Cuts at: 293 1797 3086 4193 4682 5002 5687 293
Size: 1504 1289 1107 489 320 685 2563
Fragments arranged by size:
2563 1504 1289 1107 685 489 320
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7957
PvuII CAG'CTG
Cuts at: 376 1381 2481 2502 4044 4056 4485 5380 5740
Size: 1005 1100 21 1542 12 429 895 360
Cuts at: 5740 376
Size: 2593
Fragments arranged by size:
2593 1542 1100 1005 895 429 360 21
12
RsrII CG'GwC_CG
Cuts at: 6150 6150
Size: 7957
SacI G_AGCT'C
Cuts at: 4969 4969
Size: 7957
SalI G'TCGA_C
Cuts at: 4992 4992
Size: 7957
SanDI GG'GwC_CC
Cuts at: 2245 2266 3139 2245
Size: 21 873 7063
Fragments arranged by size:
7063 873 21
SapI GCTCTTCn'nnn_
Cuts at: 2786 3976 5978 6188 2786
Size: 1190 2002 210 4555
Fragments arranged by size:
4555 2002 1190 210
SbfI CC_TGCA'GG
Cuts at: 1797 5002 1797
Size: 3205 4752
ScaI AGT'ACT
Cuts at: 3501 4090 7474 3501
Size: 589 3384 3984
Fragments arranged by size:
3984 3384 589
SfcI C'TryA_G
Cuts at: 33 289 551 1034 1793 3082 4189 4678 4711
Size: 256 262 483 759 1289 1107 489 33
Cuts at: 4711 4827 4998 5683 6678 6869 33
Size: 116 171 685 995 191 1121
Fragments arranged by size:
1289 1121 1107 995 759 685 489 483
262 256 191 171 116 33
SfoI GGC'GCC
Cuts at: 576 1445 5387 5635 576
Size: 869 3942 248 2898
Fragments arranged by size:
3942 2898 869 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7957
SgrAI Cr'CCGG_yG
Cuts at: 213 213
Size: 7957
SmaI CCC'GGG
Cuts at: 200 1914 4977 200
Size: 1714 3063 3180
Fragments arranged by size:
3180 3063 1714
SmlI C'TyrA_G
Cuts at: 797 1389 1661 2917 2941 3582 5110 6025 6519
Size: 592 272 1256 24 641 1528 915 494
Cuts at: 6519 6781 797
Size: 262 1973
Fragments arranged by size:
1973 1528 1256 915 641 592 494 272
262 24
SnaBI TAC'GTA
Cuts at: 4947 4947
Size: 7957
SpeI A'CTAG_T
Cuts at: 67 3496 67
Size: 3429 4528
SphI G_CATG'C
Cuts at: 5008 5508 5008
Size: 500 7457
StyI C'CwwG_G
Cuts at: 591 705 1421 2163 2197 3221 3884 4095 4208
Size: 114 716 742 34 1024 663 211 113
Cuts at: 4208 5089 5443 591
Size: 881 354 3105
Fragments arranged by size:
3105 1024 881 742 716 663 354 211
114 113 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 777 2326 3105 3629 4197 5538 5671 6130 6341
Size: 1549 779 524 568 1341 133 459 211
Cuts at: 6341 777
Size: 2393
Fragments arranged by size:
2393 1549 1341 779 568 524 459 211
133
TatI w'GTAC_w
Cuts at: 3499 4088 7472 3499
Size: 589 3384 3984
Fragments arranged by size:
3984 3384 589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7474 7506 7474
Size: 32 7925
Tth111I GACn'n_nGTC
Cuts at: 5752 5752
Size: 7957
XbaI T'CTAG_A
Cuts at: 4986 4986
Size: 7957
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2580 3006 5450 2580
Size: 426 2444 5087
Fragments arranged by size:
5087 2444 426
XmaI C'CCGG_G
Cuts at: 198 1912 4975 198
Size: 1714 3063 3180
Fragments arranged by size:
3180 3063 1714
XmnI GAAnn'nnTTC
Cuts at: 3511 3511
Size: 7957
ZraI GAC'GTC
Cuts at: 3059 3059
Size: 7957
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AloI AscI AvrII BsiWI BsrGI BssHII
BstEII FseI FspAI HindIII NotI PacI PfoI PsiI
PspXI PsrI SacII SexAI SfiI SrfI SspI StuI
SwaI XhoI