Restriction map for
pF1KSDB0033
(Circular) (Six-base) MAPSORT of: pF1KSDB0033.seq Check: 5138 from: 1 to: 6328
pF1KSDB0033 6328 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2646 2646
Size: 6328
AccI GT'mk_AC
Cuts at: 3364 3364
Size: 6328
Acc65I G'GTAC_C
Cuts at: 147 3342 147
Size: 3195 3133
AclI AA'CG_TT
Cuts at: 6168 6168
Size: 6328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 4151 4583 5332 5600 4151
Size: 432 749 268 4879
Fragments arranged by size:
4879 749 432 268
AflII C'TTAA_G
Cuts at: 1834 1834
Size: 6328
AflIII A'CryG_T
Cuts at: 3678 4784 5761 3678
Size: 1106 977 4245
Fragments arranged by size:
4245 1106 977
AgeI A'CCGG_T
Cuts at: 4716 4716
Size: 6328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 611 621 679 611
Size: 10 58 6260
Fragments arranged by size:
6260 58 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 691 723 691
Size: 32 6296
AlwNI CAG_nnn'CTG
Cuts at: 191 855 2334 2736 2946 3631 5200 191
Size: 664 1479 402 210 685 1569 1319
Fragments arranged by size:
1569 1479 1319 685 664 402 210
ApaI G_GGCC'C
Cuts at: 3211 3211
Size: 6328
ApaLI G'TGCA_C
Cuts at: 808 5098 808
Size: 4290 2038
ApoI r'AATT_y
Cuts at: 1747 2383 3306 3330 5681 5937 1747
Size: 636 923 24 2351 256 2138
Fragments arranged by size:
2351 2138 923 636 256 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 6328
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6328
AvaI C'yCGr_G
Cuts at: 616 1375 1627 2533 2849 3346 616
Size: 759 252 906 316 497 3598
Fragments arranged by size:
3598 906 759 497 316 252
BamHI G'GATC_C
Cuts at: 3351 3351
Size: 6328
BanI G'GyrC_C
Cuts at: 147 628 754 2892 3223 3278 3342 3756 4004
Size: 481 126 2138 331 55 64 414 248
Cuts at: 4004 4039 147
Size: 35 2436
Fragments arranged by size:
2436 2138 481 414 331 248 126 64
55 35
BanII G_rGCy'C
Cuts at: 1220 1228 1351 1382 1481 1535 1898 1985 2872
Size: 8 123 31 99 54 363 87 887
Cuts at: 2872 2879 3211 3340 4370 1220
Size: 7 332 129 1030 3178
Fragments arranged by size:
3178 1030 887 363 332 129 123 99
87 54 31 8 7
BbeI G_GCGC'C
Cuts at: 3760 4008 3760
Size: 248 6080
BbvCI CC'TCA_GC
Cuts at: 1150 1150
Size: 6328
BciVI GTATCCnnnnn_n'
Cuts at: 1662 4224 4987 6328 1662
Size: 2562 763 1341 1662
Fragments arranged by size:
2562 1662 1341 763
BclI T'GATC_A
Cuts at: 3846 3846
Size: 6328
BglII A'GATC_T
Cuts at: 407 2267 2762 3841 5880 407
Size: 1860 495 1079 2039 855
Fragments arranged by size:
2039 1860 1079 855 495
BlpI GC'TnA_GC
Cuts at: 1039 3438 1039
Size: 2399 3929
Bme1580I G_kGCm'C
Cuts at: 464 759 812 2164 3211 3281 3951 4044 5102
Size: 295 53 1352 1047 70 670 93 1058
Cuts at: 5102 464
Size: 1690
Fragments arranged by size:
1690 1352 1058 1047 670 295 93 70
53
BmgBI CAC'GTC
Cuts at: 1341 2171 1341
Size: 830 5498
BmrI ACTGGGnnnn_n'
Cuts at: 1926 2449 3538 3619 3954 6100 1926
Size: 523 1089 81 335 2146 2154
Fragments arranged by size:
2154 2146 1089 523 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1282 4693 1282
Size: 3411 2917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 688 693 877 1258 1675 1684 2335 2379
Size: 381 5 184 381 417 9 651 44
Cuts at: 2379 307
Size: 4256
Fragments arranged by size:
4256 651 417 381 381 184 44 9
5
Bpu10I CC'TnA_GC
Cuts at: 1150 1222 3040 1150
Size: 72 1818 4438
Fragments arranged by size:
4438 1818 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1471 3502 4381 4875 5173 1114
Size: 357 2031 879 494 298 2269
Fragments arranged by size:
2269 2031 879 494 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 384 595 1834 6032 384
Size: 211 1239 4198 680
Fragments arranged by size:
4198 1239 680 211
BsaAI yAC'GTr
Cuts at: 2180 3120 3318 3679 2180
Size: 940 198 361 4829
Fragments arranged by size:
4829 940 361 198
BsaBI GATnn'nnATC
Cuts at: 6 2272 3840 5879 6
Size: 2266 1568 2039 455
Fragments arranged by size:
2266 2039 1568 455
BsaHI Gr'CG_yC
Cuts at: 443 1262 2981 3757 4005 4709 6205 443
Size: 819 1719 776 248 704 1496 566
Fragments arranged by size:
1719 1496 819 776 704 566 248
BsaWI w'CCGG_w
Cuts at: 695 1826 1886 3019 3515 4036 4716 4990 5137
Size: 1131 60 1133 496 521 680 274 147
Cuts at: 5137 5548 695
Size: 411 1475
Fragments arranged by size:
1475 1133 1131 680 521 496 411 274
147 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 617 647 691 721 1567 1597 1831 1861 3086
Size: 30 44 30 846 30 234 30 1225
Cuts at: 3086 3116 617
Size: 30 3829
Fragments arranged by size:
3829 1225 846 234 44 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 883 886 942 1108 1138 1228 1516 1591 1627
Size: 3 56 166 30 90 288 75 36
Cuts at: 1627 883
Size: 5584
Fragments arranged by size:
5584 288 166 90 75 56 36 30
3
BseYI C'CCAG_C
Cuts at: 609 888 1351 3751 4509 5088 5584 609
Size: 279 463 2400 758 579 496 1353
Fragments arranged by size:
2400 1353 758 579 496 463 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 757 1744 289
Size: 468 987 4873
Fragments arranged by size:
4873 987 468
BsiEI CG_ry'CG
Cuts at: 104 1925 2031 2194 2913 3868 3914 5124 104
Size: 1821 106 163 719 955 46 1210 1308
Fragments arranged by size:
1821 1308 1210 955 719 163 106 46
BsiHKAI G_wGCw'C
Cuts at: 504 812 1220 1366 1481 1898 3340 4118 4308
Size: 308 408 146 115 417 1442 778 190
Cuts at: 4308 5102 504
Size: 794 1730
Fragments arranged by size:
1730 1442 794 778 417 408 308 190
146 115
BsiWI C'GTAC_G
Cuts at: 1250 1250
Size: 6328
BsmBI CGTCTCn'nnnn_
Cuts at: 1253 1912 2161 3121 1253
Size: 659 249 960 4460
Fragments arranged by size:
4460 960 659 249
Bsp1286I G_dGCh'C
Cuts at: 464 504 759 812 1220 1228 1351 1366 1382
Size: 40 255 53 408 8 123 15 16
Cuts at: 1382 1481 1535 1898 1985 2164 2872 2879 3211
Size: 99 54 363 87 179 708 7 332
Cuts at: 3211 3281 3340 3951 4044 4118 4308 4370 5102
Size: 70 59 611 93 74 190 62 732
Cuts at: 5102 464
Size: 1690
Fragments arranged by size:
1690 732 708 611 408 363 332 255
190 179 123 99 93 87 74 70
62 59 54 53 40 16 15 8
7
BspEI T'CCGG_A
Cuts at: 695 1826 5548 695
Size: 1131 3722 1475
Fragments arranged by size:
3722 1475 1131
BspHI T'CATG_A
Cuts at: 5504 5504
Size: 6328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 410 613 704 1043 2096 2646 3376 3590
Size: 78 203 91 339 1053 550 730 214
Cuts at: 3590 3892 4273 332
Size: 302 381 2387
Fragments arranged by size:
2387 1053 730 550 381 339 302 214
203 91 78
BsrBI CCG'CTC
Cuts at: 50 551 828 4618 4672 6158 6323 50
Size: 501 277 3790 54 1486 165 55
Fragments arranged by size:
3790 1486 501 277 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1107 4238 1107
Size: 3131 3197
BsrFI r'CCGG_y
Cuts at: 416 641 2104 4324 4505 4716 416
Size: 225 1463 2220 181 211 2028
Fragments arranged by size:
2220 2028 1463 225 211 181
BsrGI T'GTAC_A
Cuts at: 1280 3066 1280
Size: 1786 4542
BssSI C'ACGA_G
Cuts at: 800 1256 2619 4597 4957 800
Size: 456 1363 1978 360 2171
Fragments arranged by size:
2171 1978 1363 456 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2736 2946 3703 2736
Size: 210 757 5361
Fragments arranged by size:
5361 757 210
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6328
BstEII G'GTnAC_C
Cuts at: 225 1110 1776 225
Size: 885 666 4777
Fragments arranged by size:
4777 885 666
BstXI CCAn_nnnn'nTGG
Cuts at: 820 1341 2171 820
Size: 521 830 4977
Fragments arranged by size:
4977 830 521
BstYI r'GATC_y
Cuts at: 1 407 1964 2267 2345 2762 3351 3818 3841
Size: 406 1557 303 78 417 589 467 23
Cuts at: 3841 4176 4422 5436 5522 5534 5880 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1557 1014 589 467 449 417 406 346
335 303 246 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 2584 2584
Size: 6328
BtgI C'CryG_G
Cuts at: 125 230 438 479 761 1367 2750 3298 125
Size: 105 208 41 282 606 1383 548 3155
Fragments arranged by size:
3155 1383 606 548 282 208 105 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1185 1506 4276 4457 4620 1185
Size: 321 2770 181 163 2893
Fragments arranged by size:
2893 2770 321 181 163
BtsI GCAGTG_nn'
Cuts at: 216 502 3569 216
Size: 286 3067 2975
Fragments arranged by size:
3067 2975 286
DraI TTT'AAA
Cuts at: 3325 3325
Size: 6328
DraIII CAC_nnn'GTG
Cuts at: 2061 2621 2061
Size: 560 5768
DrdI GACnn_nn'nnGTC
Cuts at: 979 2986 4032 4892 979
Size: 2007 1046 860 2415
Fragments arranged by size:
2415 2007 1046 860
EaeI y'GGCC_r
Cuts at: 768 1049 1912 2028 2191 2336 3911 4085 4476
Size: 281 863 116 163 145 1575 174 391
Cuts at: 4476 4503 5850 768
Size: 27 1347 1246
Fragments arranged by size:
1575 1347 1246 863 391 281 174 163
145 116 27
EagI C'GGCC_G
Cuts at: 2028 2191 3911 2028
Size: 163 1720 4445
Fragments arranged by size:
4445 1720 163
EarI CTCTTCn'nnn_
Cuts at: 884 1474 1828 2237 2392 2815 4349 4559 884
Size: 590 354 409 155 423 1534 210 2653
Fragments arranged by size:
2653 1534 590 423 409 354 210 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 499 1374 1741 2041 4534 4846 4992 6138 499
Size: 875 367 300 2493 312 146 1146 689
Fragments arranged by size:
2493 1146 875 689 367 312 300 146
EcoICRI GAG'CTC
Cuts at: 1218 1479 1896 3338 1218
Size: 261 417 1442 4208
Fragments arranged by size:
4208 1442 417 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 688 693 877 1258 1675 1684 2335 2379
Size: 381 5 184 381 417 9 651 44
Cuts at: 2379 4151 4583 5332 5600 307
Size: 1772 432 749 268 1035
Fragments arranged by size:
1772 1035 749 651 432 417 381 381
268 184 44 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 928 1240 1270 2647 928
Size: 312 30 1377 4609
Fragments arranged by size:
4609 1377 312 30
EcoO109I rG'GnC_Cy
Cuts at: 188 399 1032 3207 3208 3465 188
Size: 211 633 2175 1 257 3051
Fragments arranged by size:
3051 2175 633 257 211 1
EcoRI G'AATT_C
Cuts at: 1747 3306 3330 1747
Size: 1559 24 4745
Fragments arranged by size:
4745 1559 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4345 4345
Size: 6328
FspI TGC'GCA
Cuts at: 508 2098 2563 4107 508
Size: 1590 465 1544 2729
Fragments arranged by size:
2729 1590 1544 465
FspAI rTGC'GCAy
Cuts at: 2563 2563
Size: 6328
HaeII r_GCGC'y
Cuts at: 133 3760 4008 5032 6011 133
Size: 3627 248 1024 979 450
Fragments arranged by size:
3627 1024 979 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2393 2425 2492 2524 3033 3065 4168 4198 4200
Size: 32 67 32 509 32 1103 30 2
Cuts at: 4200 4230 2393
Size: 30 4491
Fragments arranged by size:
4491 1103 509 67 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3365 3365
Size: 6328
HindIII A'AGCT_T
Cuts at: 196 2132 196
Size: 1936 4392
KasI G'GCGC_C
Cuts at: 3756 4004 3756
Size: 248 6080
KpnI G_GTAC'C
Cuts at: 151 3346 151
Size: 3195 3133
MluI A'CGCG_T
Cuts at: 5761 5761
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1472 2060 2244 4999 5183 1472
Size: 588 184 2755 184 2617
Fragments arranged by size:
2755 2617 588 184 184
MscI TGG'CCA
Cuts at: 770 1051 2338 4087 770
Size: 281 1287 1749 3011
Fragments arranged by size:
3011 1749 1287 281
MslI CAynn'nnrTG
Cuts at: 169 733 4315 4442 169
Size: 564 3582 127 2055
Fragments arranged by size:
3582 2055 564 127
MspA1I CmG'CkG
Cuts at: 852 1230 1355 1719 1956 1997 2528 2595 2736
Size: 378 125 364 237 41 531 67 141
Cuts at: 2736 2943 3437 3751 4111 5126 5371 852
Size: 207 494 314 360 1015 245 1809
Fragments arranged by size:
1809 1015 531 494 378 364 360 314
245 237 207 141 125 67 41
NaeI GCC'GGC
Cuts at: 418 2106 4507 418
Size: 1688 2401 2239
Fragments arranged by size:
2401 2239 1688
NarI GG'CG_CC
Cuts at: 3757 4005 3757
Size: 248 6080
NcoI C'CATG_G
Cuts at: 125 230 125
Size: 105 6223
NdeI CA'TA_TG
Cuts at: 425 425
Size: 6328
NgoMIV G'CCGG_C
Cuts at: 416 2104 4505 416
Size: 1688 2401 2239
Fragments arranged by size:
2401 2239 1688
NruI TCG'CGA
Cuts at: 4689 4689
Size: 6328
NspI r_CATG'y
Cuts at: 3379 3879 4788 3379
Size: 500 909 4919
Fragments arranged by size:
4919 909 500
PasI CC'CwG_GG
Cuts at: 204 204
Size: 6328
PciI A'CATG_T
Cuts at: 4784 4784
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 855 855
Size: 6328
PfoI T'CCnGG_A
Cuts at: 788 1382 1652 2549 2609 788
Size: 594 270 897 60 4507
Fragments arranged by size:
4507 897 594 270 60
PmeI GTTT'AAAC
Cuts at: 3325 3325
Size: 6328
PmlI CAC'GTG
Cuts at: 2180 3120 2180
Size: 940 5388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 691 723 5467 5499 691
Size: 32 4744 32 1520
Fragments arranged by size:
4744 1520 32 32
PpuMI rG'GwC_Cy
Cuts at: 399 1032 399
Size: 633 5695
PspOMI G'GGCC_C
Cuts at: 3207 3207
Size: 6328
PstI C_TGCA'G
Cuts at: 407 590 1463 1955 1973 2741 2861 3110 3373
Size: 183 873 492 18 768 120 249 263
Cuts at: 3373 4058 407
Size: 685 2677
Fragments arranged by size:
2677 873 768 685 492 263 249 183
120 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6328
PvuII CAG'CTG
Cuts at: 852 1355 1956 2595 2736 2943 3751 4111 852
Size: 503 601 639 141 207 808 360 3069
Fragments arranged by size:
3069 808 639 601 503 360 207 141
RsrII CG'GwC_CG
Cuts at: 4521 4521
Size: 6328
SacI G_AGCT'C
Cuts at: 1220 1481 1898 3340 1220
Size: 261 417 1442 4208
Fragments arranged by size:
4208 1442 417 261
SalI G'TCGA_C
Cuts at: 3363 3363
Size: 6328
SapI GCTCTTCn'nnn_
Cuts at: 1474 2815 4349 4559 1474
Size: 1341 1534 210 3243
Fragments arranged by size:
3243 1534 1341 210
SbfI CC_TGCA'GG
Cuts at: 3373 3373
Size: 6328
ScaI AGT'ACT
Cuts at: 5845 5845
Size: 6328
SexAI A'CCwGG_T
Cuts at: 923 2057 923
Size: 1134 5194
SfcI C'TryA_G
Cuts at: 33 403 411 586 649 1459 1782 1951 1969
Size: 370 8 175 63 810 323 169 18
Cuts at: 1969 2737 2857 3106 3369 4054 5049 5240 33
Size: 768 120 249 263 685 995 191 1121
Fragments arranged by size:
1121 995 810 768 685 370 323 263
249 191 175 169 120 63 18 8
SfoI GGC'GCC
Cuts at: 3758 4006 3758
Size: 248 6080
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6328
SmaI CCC'GGG
Cuts at: 1629 3348 1629
Size: 1719 4609
SmlI C'TyrA_G
Cuts at: 1129 1486 1834 3481 4396 4890 5152 1129
Size: 357 348 1647 915 494 262 2305
Fragments arranged by size:
2305 1647 915 494 357 348 262
SnaBI TAC'GTA
Cuts at: 3318 3318
Size: 6328
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6328
SphI G_CATG'C
Cuts at: 3379 3879 3379
Size: 500 5828
StuI AGG'CCT
Cuts at: 673 2653 2863 673
Size: 1980 210 4138
Fragments arranged by size:
4138 1980 210
StyI C'CwwG_G
Cuts at: 125 230 1205 1271 1334 2864 3263 3460 3814
Size: 105 975 66 63 1530 399 197 354
Cuts at: 3814 125
Size: 2639
Fragments arranged by size:
2639 1530 975 399 354 197 105 66
63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 754 1945 3909 4042 4501 4712 580
Size: 174 1191 1964 133 459 211 2196
Fragments arranged by size:
2196 1964 1191 459 211 174 133
TatI w'GTAC_w
Cuts at: 725 1280 2246 3066 5843 725
Size: 555 966 820 2777 1210
Fragments arranged by size:
2777 1210 966 820 555
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 617 649 1600 1632 1831 1863 2468 2500 2605
Size: 32 951 32 199 32 605 32 105
Cuts at: 2605 2637 3086 3118 5845 5877 617
Size: 32 449 32 2727 32 1068
Fragments arranged by size:
2727 1068 951 605 449 199 105 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4123 4123
Size: 6328
XbaI T'CTAG_A
Cuts at: 3357 3357
Size: 6328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1731 3821 1731
Size: 2090 4238
XmaI C'CCGG_G
Cuts at: 1627 3346 1627
Size: 1719 4609
XmnI GAAnn'nnTTC
Cuts at: 1406 2542 1406
Size: 1136 5192
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AscI AvrII BaeI BbsI
BcgI BfrBI BglI BmtI BsmI BssHII BstZ17I ClaI
CspCI EcoRV FseI HpaI MfeI NheI NotI NsiI
PacI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SspI SwaI XhoI ZraI