Restriction map for
pF1KSDB0034
(Circular) (Six-base) MAPSORT of: pF1KSDB0034.seq Check: 9451 from: 1 to: 5248
pF1KSDB0034 5248 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 873 1742 1848 1935 2151 873
Size: 869 106 87 216 3970
Fragments arranged by size:
3970 869 216 106 87
AccI GT'mk_AC
Cuts at: 1333 2284 1333
Size: 951 4297
Acc65I G'GTAC_C
Cuts at: 2262 2262
Size: 5248
AclI AA'CG_TT
Cuts at: 5088 5088
Size: 5248
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 763 787 1216 1447 3071 3503 4252 4520
Size: 517 24 429 231 1624 432 749 268
Cuts at: 4520 246
Size: 974
Fragments arranged by size:
1624 974 749 517 432 429 268 231
24
AflIII A'CryG_T
Cuts at: 1601 2598 3704 4681 1601
Size: 997 1106 977 2168
Fragments arranged by size:
2168 1106 997 977
AgeI A'CCGG_T
Cuts at: 3636 3636
Size: 5248
AleI CACnn'nnGTG
Cuts at: 376 1075 1276 376
Size: 699 201 4348
Fragments arranged by size:
4348 699 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 509 1011 1471 1723 169
Size: 340 502 460 252 3694
Fragments arranged by size:
3694 502 460 340 252
AlwNI CAG_nnn'CTG
Cuts at: 705 1362 1560 2551 4120 705
Size: 657 198 991 1569 1833
Fragments arranged by size:
1833 1569 991 657 198
ApaI G_GGCC'C
Cuts at: 616 736 1376 2025 2129 616
Size: 120 640 649 104 3735
Fragments arranged by size:
3735 649 640 120 104
ApaLI G'TGCA_C
Cuts at: 4018 4018
Size: 5248
ApoI r'AATT_y
Cuts at: 2250 4601 4857 2250
Size: 2351 256 2641
Fragments arranged by size:
2641 2351 256
AscI GG'CGCG_CC
Cuts at: 1848 1848
Size: 5248
AseI AT'TA_AT
Cuts at: 20 20
Size: 5248
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5248
AvaI C'yCGr_G
Cuts at: 210 2059 2266 210
Size: 1849 207 3192
Fragments arranged by size:
3192 1849 207
BamHI G'GATC_C
Cuts at: 800 2271 800
Size: 1471 3777
BanI G'GyrC_C
Cuts at: 183 667 1747 2262 2676 2924 2959 183
Size: 484 1080 515 414 248 35 2472
Fragments arranged by size:
2472 1080 515 484 414 248 35
BanII G_rGCy'C
Cuts at: 398 616 736 755 1296 1376 1993 2025 2129
Size: 218 120 19 541 80 617 32 104
Cuts at: 2129 2260 3290 398
Size: 131 1030 2356
Fragments arranged by size:
2356 1030 617 541 218 131 120 104
80 32 19
BbeI G_GCGC'C
Cuts at: 1751 2680 2928 1751
Size: 929 248 4071
Fragments arranged by size:
4071 929 248
BbsI GAAGACnn'nnnn_
Cuts at: 198 1080 198
Size: 882 4366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1558 1592 2194 2228 1558
Size: 34 602 34 4578
Fragments arranged by size:
4578 602 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3144 3907 5248 3144
Size: 763 1341 3144
Fragments arranged by size:
3144 1341 763
BclI T'GATC_A
Cuts at: 2766 2766
Size: 5248
BglI GCCn_nnn'nGGC
Cuts at: 901 2062 901
Size: 1161 4087
BglII A'GATC_T
Cuts at: 806 2761 4800 806
Size: 1955 2039 1254
Fragments arranged by size:
2039 1955 1254
BlpI GC'TnA_GC
Cuts at: 2358 2358
Size: 5248
Bme1580I G_kGCm'C
Cuts at: 148 275 444 460 616 736 1376 2025 2129
Size: 127 169 16 156 120 640 649 104
Cuts at: 2129 2871 2964 4022 148
Size: 742 93 1058 1374
Fragments arranged by size:
1374 1058 742 649 640 169 156 127
120 104 93 16
BmgBI CAC'GTC
Cuts at: 374 1274 374
Size: 900 4348
BmrI ACTGGGnnnn_n'
Cuts at: 1770 2458 2539 2874 5020 1770
Size: 688 81 335 2146 1998
Fragments arranged by size:
2146 1998 688 335 81
BmtI G_CTAG'C
Cuts at: 1910 1910
Size: 5248
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2085 3613 2085
Size: 1528 3720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1057 1435 2074 2174 412
Size: 645 378 639 100 3486
Fragments arranged by size:
3486 645 639 378 100
Bpu10I CC'TnA_GC
Cuts at: 706 737 706
Size: 31 5217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1495 2422 3301 3795 4093 436
Size: 1059 927 879 494 298 1591
Fragments arranged by size:
1591 1059 927 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1336 1869 4952 1336
Size: 533 3083 1632
Fragments arranged by size:
3083 1632 533
BsaAI yAC'GTr
Cuts at: 1604 2238 2599 1604
Size: 634 361 4253
Fragments arranged by size:
4253 634 361
BsaBI GATnn'nnATC
Cuts at: 6 805 1495 2760 4799 6
Size: 799 690 1265 2039 455
Fragments arranged by size:
2039 1265 799 690 455
BsaHI Gr'CG_yC
Cuts at: 815 1748 2677 2925 3629 5125 815
Size: 933 929 248 704 1496 938
Fragments arranged by size:
1496 938 933 929 704 248
BsaWI w'CCGG_w
Cuts at: 2435 2956 3636 3910 4057 4468 2435
Size: 521 680 274 147 411 3215
Fragments arranged by size:
3215 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 667 697 853 883 1156 1186 1375 1405 2147
Size: 30 156 30 273 30 189 30 742
Cuts at: 2147 2177 667
Size: 30 3738
Fragments arranged by size:
3738 742 273 189 156 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 739 874 1399 1624 1783 1897 2058 739
Size: 135 525 225 159 114 161 3929
Fragments arranged by size:
3929 525 225 161 159 135 114
BseYI C'CCAG_C
Cuts at: 164 729 1149 1362 1801 1807 2029 2042 2137
Size: 565 420 213 439 6 222 13 95
Cuts at: 2137 2173 2671 3429 4008 4504 164
Size: 36 498 758 579 496 908
Fragments arranged by size:
908 758 579 565 498 496 439 420
222 213 95 36 13 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 718 898 907 1336 1690 1714 2234 2242
Size: 344 180 9 429 354 24 520 8
Cuts at: 2242 374
Size: 3380
Fragments arranged by size:
3380 520 429 354 344 180 24 9
8
BsiEI CG_ry'CG
Cuts at: 104 2788 2834 4044 104
Size: 2684 46 1210 1308
Fragments arranged by size:
2684 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 296 398 755 950 2260 3038 3228 4022 296
Size: 102 357 195 1310 778 190 794 1522
Fragments arranged by size:
1522 1310 794 778 357 195 190 102
BsiWI C'GTAC_G
Cuts at: 1604 1604
Size: 5248
Bsp1286I G_dGCh'C
Cuts at: 148 275 296 398 444 460 616 736 755
Size: 127 21 102 46 16 156 120 19
Cuts at: 755 950 1296 1376 1993 2025 2129 2260 2871
Size: 195 346 80 617 32 104 131 611
Cuts at: 2871 2964 3038 3228 3290 4022 148
Size: 93 74 190 62 732 1374
Fragments arranged by size:
1374 732 617 611 346 195 190 156
131 127 120 104 102 93 80 74
62 46 32 21 19 16
BspEI T'CCGG_A
Cuts at: 4468 4468
Size: 5248
BspHI T'CATG_A
Cuts at: 1631 4424 1631
Size: 2793 2455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 315 873 1537 1736 1742 1848 1935 2151 2296
Size: 558 664 199 6 106 87 216 145
Cuts at: 2296 2510 2812 3193 315
Size: 214 302 381 2370
Fragments arranged by size:
2370 664 558 381 302 216 214 199
145 106 87 6
BsrBI CCG'CTC
Cuts at: 50 321 474 786 1419 3538 3592 5078 5243
Size: 271 153 312 633 2119 54 1486 165
Cuts at: 5243 50
Size: 55
Fragments arranged by size:
2119 1486 633 312 271 165 153 55
54
BsrDI GCAATG_nn'
Cuts at: 3158 3158
Size: 5248
BsrFI r'CCGG_y
Cuts at: 891 2002 3244 3425 3636 891
Size: 1111 1242 181 211 2503
Fragments arranged by size:
2503 1242 1111 211 181
BssHII G'CGCG_C
Cuts at: 850 1848 850
Size: 998 4250
BssSI C'ACGA_G
Cuts at: 268 335 3517 3877 268
Size: 67 3182 360 1639
Fragments arranged by size:
3182 1639 360 67
BstAPI GCAn_nnn'nTGC
Cuts at: 552 885 2100 2623 552
Size: 333 1215 523 3177
Fragments arranged by size:
3177 1215 523 333
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5248
BstXI CCAn_nnnn'nTGG
Cuts at: 1860 2152 1860
Size: 292 4956
BstYI r'GATC_y
Cuts at: 1 701 800 806 962 1298 1952 2271 2738
Size: 700 99 6 156 336 654 319 467
Cuts at: 2738 2761 3096 3342 4356 4442 4454 4800 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 700 654 467 449 346 336 335
319 246 156 99 86 23 12 6
BstZ17I GTA'TAC
Cuts at: 1334 1334
Size: 5248
Bsu36I CC'TnA_GG
Cuts at: 593 2048 593
Size: 1455 3793
BtgI C'CryG_G
Cuts at: 377 422 760 1664 377
Size: 45 338 904 3961
Fragments arranged by size:
3961 904 338 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3196 3377 3540 3196
Size: 181 163 4904
Fragments arranged by size:
4904 181 163
BtsI GCAGTG_nn'
Cuts at: 444 573 802 2489 444
Size: 129 229 1687 3203
Fragments arranged by size:
3203 1687 229 129
DraI TTT'AAA
Cuts at: 2245 2245
Size: 5248
DraIII CAC_nnn'GTG
Cuts at: 831 831
Size: 5248
DrdI GACnn_nn'nnGTC
Cuts at: 1702 2952 3812 1702
Size: 1250 860 3138
Fragments arranged by size:
3138 1250 860
EaeI y'GGCC_r
Cuts at: 893 1739 2831 3005 3396 3423 4770 893
Size: 846 1092 174 391 27 1347 1371
Fragments arranged by size:
1371 1347 1092 846 391 174 27
EagI C'GGCC_G
Cuts at: 2831 2831
Size: 5248
EarI CTCTTCn'nnn_
Cuts at: 226 1456 3269 3479 226
Size: 1230 1813 210 1995
Fragments arranged by size:
1995 1813 1230 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3454 3766 3912 5058 3454
Size: 312 146 1146 3644
Fragments arranged by size:
3644 1146 312 146
EcoICRI GAG'CTC
Cuts at: 396 753 2258 396
Size: 357 1505 3386
Fragments arranged by size:
3386 1505 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 412 763 787 1057 1216 1435 1447 2074
Size: 166 351 24 270 159 219 12 627
Cuts at: 2074 2174 3071 3503 4252 4520 246
Size: 100 897 432 749 268 974
Fragments arranged by size:
974 897 749 627 432 351 270 268
219 166 159 100 24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 982 1354 982
Size: 372 4876
EcoO109I rG'GnC_Cy
Cuts at: 327 612 613 733 1041 1372 1410 2021 2022
Size: 285 1 120 308 331 38 611 1
Cuts at: 2022 2126 2385 327
Size: 104 259 3190
Fragments arranged by size:
3190 611 331 308 285 259 120 104
38 1 1
EcoRI G'AATT_C
Cuts at: 2250 2250
Size: 5248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 870 1485 3265 870
Size: 615 1780 2853
Fragments arranged by size:
2853 1780 615
FspI TGC'GCA
Cuts at: 3027 3027
Size: 5248
HaeII r_GCGC'y
Cuts at: 1483 1751 2680 2928 3952 4931 1483
Size: 268 929 248 1024 979 1800
Fragments arranged by size:
1800 1024 979 929 268 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 329 361 853 885 959 991 1156 1188 1283
Size: 32 492 32 74 32 165 32 95
Cuts at: 1283 1315 1435 1467 2149 2181 3088 3118 3120
Size: 32 120 32 682 32 907 30 2
Cuts at: 3120 3150 329
Size: 30 2427
Fragments arranged by size:
2427 907 682 492 165 120 95 74
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2285 2285
Size: 5248
KasI G'GCGC_C
Cuts at: 1747 2676 2924 1747
Size: 929 248 4071
Fragments arranged by size:
4071 929 248
KpnI G_GTAC'C
Cuts at: 2266 2266
Size: 5248
MluI A'CGCG_T
Cuts at: 4681 4681
Size: 5248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1517 3919 4103 1517
Size: 2402 184 2662
Fragments arranged by size:
2662 2402 184
MscI TGG'CCA
Cuts at: 3007 3007
Size: 5248
MslI CAynn'nnrTG
Cuts at: 376 491 637 1075 1108 1276 1591 1705 3235
Size: 115 146 438 33 168 315 114 1530
Cuts at: 3235 3362 376
Size: 127 2262
Fragments arranged by size:
2262 1530 438 315 168 146 127 115
114 33
MspA1I CmG'CkG
Cuts at: 168 762 792 1407 1666 1784 2029 2357 2671
Size: 594 30 615 259 118 245 328 314
Cuts at: 2671 3031 4046 4291 168
Size: 360 1015 245 1125
Fragments arranged by size:
1125 1015 615 594 360 328 314 259
245 245 118 30
NaeI GCC'GGC
Cuts at: 893 2004 3427 893
Size: 1111 1423 2714
Fragments arranged by size:
2714 1423 1111
NarI GG'CG_CC
Cuts at: 1748 2677 2925 1748
Size: 929 248 4071
Fragments arranged by size:
4071 929 248
NgoMIV G'CCGG_C
Cuts at: 891 2002 3425 891
Size: 1111 1423 2714
Fragments arranged by size:
2714 1423 1111
NheI G'CTAG_C
Cuts at: 1906 1906
Size: 5248
NruI TCG'CGA
Cuts at: 3609 3609
Size: 5248
NspI r_CATG'y
Cuts at: 642 1308 2299 2799 3708 642
Size: 666 991 500 909 2182
Fragments arranged by size:
2182 991 909 666 500
PciI A'CATG_T
Cuts at: 3704 3704
Size: 5248
PflMI CCAn_nnn'nTGG
Cuts at: 390 1362 1760 390
Size: 972 398 3878
Fragments arranged by size:
3878 972 398
PfoI T'CCnGG_A
Cuts at: 1034 1412 1472 1553 1874 1034
Size: 378 60 81 321 4408
Fragments arranged by size:
4408 378 321 81 60
PmeI GTTT'AAAC
Cuts at: 2245 2245
Size: 5248
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4387 4419 4387
Size: 32 5216
PpuMI rG'GwC_Cy
Cuts at: 327 1410 327
Size: 1083 4165
PspOMI G'GGCC_C
Cuts at: 612 732 1372 2021 2125 612
Size: 120 640 649 104 3735
Fragments arranged by size:
3735 649 640 120 104
PstI C_TGCA'G
Cuts at: 560 1250 1490 1625 1649 2293 2978 560
Size: 690 240 135 24 644 685 2830
Fragments arranged by size:
2830 690 685 644 240 135 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5248
PvuII CAG'CTG
Cuts at: 168 2671 3031 168
Size: 2503 360 2385
Fragments arranged by size:
2503 2385 360
RsrII CG'GwC_CG
Cuts at: 3441 3441
Size: 5248
SacI G_AGCT'C
Cuts at: 398 755 2260 398
Size: 357 1505 3386
Fragments arranged by size:
3386 1505 357
SacII CC_GC'GG
Cuts at: 763 1667 763
Size: 904 4344
SalI G'TCGA_C
Cuts at: 2283 2283
Size: 5248
SapI GCTCTTCn'nnn_
Cuts at: 3269 3479 3269
Size: 210 5038
SbfI CC_TGCA'GG
Cuts at: 1649 2293 1649
Size: 644 4604
ScaI AGT'ACT
Cuts at: 4765 4765
Size: 5248
SexAI A'CCwGG_T
Cuts at: 713 713
Size: 5248
SfcI C'TryA_G
Cuts at: 33 556 1246 1486 1621 1645 2289 2974 3969
Size: 523 690 240 135 24 644 685 995
Cuts at: 3969 4160 33
Size: 191 1121
Fragments arranged by size:
1121 995 690 685 644 523 240 191
135 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 901 901
Size: 5248
SfoI GGC'GCC
Cuts at: 1749 2678 2926 1749
Size: 929 248 4071
Fragments arranged by size:
4071 929 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5248
SmaI CCC'GGG
Cuts at: 2268 2268
Size: 5248
SmlI C'TyrA_G
Cuts at: 451 1510 2401 3316 3810 4072 451
Size: 1059 891 915 494 262 1627
Fragments arranged by size:
1627 1059 915 891 494 262
SnaBI TAC'GTA
Cuts at: 2238 2238
Size: 5248
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5248
SphI G_CATG'C
Cuts at: 2299 2799 2299
Size: 500 4748
StuI AGG'CCT
Cuts at: 1961 2065 1961
Size: 104 5144
StyI C'CwwG_G
Cuts at: 602 653 845 872 941 983 1379 1971 2121
Size: 51 192 27 69 42 396 592 150
Cuts at: 2121 2380 2734 602
Size: 259 354 3116
Fragments arranged by size:
3116 592 396 354 259 192 150 69
51 42 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2829 2962 3421 3632 2829
Size: 133 459 211 4445
Fragments arranged by size:
4445 459 211 133
TatI w'GTAC_w
Cuts at: 1157 1772 4763 1157
Size: 615 2991 1642
Fragments arranged by size:
2991 1642 615
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 667 699 1375 1407 2099 2131 2147 2179 4765
Size: 32 676 32 692 32 16 32 2586
Cuts at: 4765 4797 667
Size: 32 1118
Fragments arranged by size:
2586 1118 692 676 32 32 32 32
32 16
Tth111I GACn'n_nGTC
Cuts at: 1942 3043 1942
Size: 1101 4147
XbaI T'CTAG_A
Cuts at: 2277 2277
Size: 5248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1587 2741 1587
Size: 1154 4094
XmaI C'CCGG_G
Cuts at: 2266 2266
Size: 5248
XmnI GAAnn'nnTTC
Cuts at: 220 1465 220
Size: 1245 4003
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NruI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AvrII BaeI BbvCI
BfrBI BsmI BsmBI BsrGI BstEII ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NotI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SanDI SgrAI SrfI SspI SwaI XhoI ZraI