Restriction map for
pF1KSDB0035
(Circular) (Six-base) MAPSORT of: pF1KSDB0035.seq Check: 1806 from: 1 to: 4546
pF1KSDB0035 4546 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1582 1582
Size: 4546
Acc65I G'GTAC_C
Cuts at: 362 765 1158 1560 362
Size: 403 393 402 3348
Fragments arranged by size:
3348 403 402 393
AclI AA'CG_TT
Cuts at: 4386 4386
Size: 4546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1289 2369 2801 3550 3818 1289
Size: 1080 432 749 268 2017
Fragments arranged by size:
2017 1080 749 432 268
AflIII A'CryG_T
Cuts at: 1896 3002 3979 1896
Size: 1106 977 2463
Fragments arranged by size:
2463 1106 977
AgeI A'CCGG_T
Cuts at: 2934 2934
Size: 4546
AleI CACnn'nnGTG
Cuts at: 372 372
Size: 4546
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 552 584 552
Size: 32 4514
AlwNI CAG_nnn'CTG
Cuts at: 931 1143 1189 1213 1849 3418 931
Size: 212 46 24 636 1569 2059
Fragments arranged by size:
2059 1569 636 212 46 24
ApaLI G'TGCA_C
Cuts at: 3316 3316
Size: 4546
ApoI r'AATT_y
Cuts at: 216 399 542 1376 1405 1432 1548 3899 4155
Size: 183 143 834 29 27 116 2351 256
Cuts at: 4155 216
Size: 607
Fragments arranged by size:
2351 834 607 256 183 143 116 29
27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4546
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4546
AvaI C'yCGr_G
Cuts at: 839 1564 839
Size: 725 3821
BamHI G'GATC_C
Cuts at: 1569 1569
Size: 4546
BanI G'GyrC_C
Cuts at: 362 765 883 1158 1273 1560 1974 2222 2257
Size: 403 118 275 115 287 414 248 35
Cuts at: 2257 362
Size: 2651
Fragments arranged by size:
2651 414 403 287 275 248 118 115
35
BanII G_rGCy'C
Cuts at: 749 1558 2588 749
Size: 809 1030 2707
Fragments arranged by size:
2707 1030 809
BbeI G_GCGC'C
Cuts at: 1978 2226 1978
Size: 248 4298
BbsI GAAGACnn'nnnn_
Cuts at: 319 659 852 319
Size: 340 193 4013
Fragments arranged by size:
4013 340 193
BciVI GTATCCnnnnn_n'
Cuts at: 2442 3205 4546 2442
Size: 763 1341 2442
Fragments arranged by size:
2442 1341 763
BclI T'GATC_A
Cuts at: 2064 2064
Size: 4546
BglII A'GATC_T
Cuts at: 2059 4098 2059
Size: 2039 2507
BlpI GC'TnA_GC
Cuts at: 1656 1656
Size: 4546
Bme1580I G_kGCm'C
Cuts at: 886 1278 2169 2262 3320 886
Size: 392 891 93 1058 2112
Fragments arranged by size:
2112 1058 891 392 93
BmrI ACTGGGnnnn_n'
Cuts at: 896 1756 1837 2172 4318 896
Size: 860 81 335 2146 1124
Fragments arranged by size:
2146 1124 860 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 1185 953
Size: 232 4314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1720 2599 3093 3391 1024
Size: 696 879 494 298 2179
Fragments arranged by size:
2179 879 696 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4250 4250
Size: 4546
BsaAI yAC'GTr
Cuts at: 1536 1897 1536
Size: 361 4185
BsaBI GATnn'nnATC
Cuts at: 6 2058 4097 6
Size: 2052 2039 455
Fragments arranged by size:
2052 2039 455
BsaHI Gr'CG_yC
Cuts at: 1975 2223 2927 4423 1975
Size: 248 704 1496 2098
Fragments arranged by size:
2098 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1257 1733 2254 2934 3208 3355 3766 1257
Size: 476 521 680 274 147 411 2037
Fragments arranged by size:
2037 680 521 476 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1015 1045 1015
Size: 30 4516
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 818 839 818
Size: 21 4525
BseYI C'CCAG_C
Cuts at: 1969 2727 3306 3802 1969
Size: 758 579 496 2713
Fragments arranged by size:
2713 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 2086 2132 3342 104
Size: 1982 46 1210 1308
Fragments arranged by size:
1982 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 749 1558 2336 2526 3320 749
Size: 809 778 190 794 1975
Fragments arranged by size:
1975 809 794 778 190
BsmI GAATG_Cn'
Cuts at: 156 156
Size: 4546
Bsp1286I G_dGCh'C
Cuts at: 749 886 1278 1558 2169 2262 2336 2526 2588
Size: 137 392 280 611 93 74 190 62
Cuts at: 2588 3320 749
Size: 732 1975
Fragments arranged by size:
1975 732 611 392 280 190 137 93
74 62
BspEI T'CCGG_A
Cuts at: 3766 3766
Size: 4546
BspHI T'CATG_A
Cuts at: 3722 3722
Size: 4546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1594 1808 2110 2491 1594
Size: 214 302 381 3649
Fragments arranged by size:
3649 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2836 2890 4376 4541 50
Size: 2786 54 1486 165 55
Fragments arranged by size:
2786 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1326 2456 1326
Size: 1130 3416
BsrFI r'CCGG_y
Cuts at: 2542 2723 2934 2542
Size: 181 211 4154
Fragments arranged by size:
4154 211 181
BssSI C'ACGA_G
Cuts at: 2815 3175 2815
Size: 360 4186
BstAPI GCAn_nnn'nTGC
Cuts at: 1921 1921
Size: 4546
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4546
BstXI CCAn_nnnn'nTGG
Cuts at: 374 554 374
Size: 180 4366
BstYI r'GATC_y
Cuts at: 1 560 727 989 1569 2036 2059 2394 2640
Size: 559 167 262 580 467 23 335 246
Cuts at: 2640 3654 3740 3752 4098 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 580 559 467 449 346 335 262
246 167 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1154 1468 1154
Size: 314 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2494 2675 2838 2494
Size: 181 163 4202
Fragments arranged by size:
4202 181 163
BtsI GCAGTG_nn'
Cuts at: 1299 1787 1299
Size: 488 4058
DraI TTT'AAA
Cuts at: 1543 1543
Size: 4546
DraIII CAC_nnn'GTG
Cuts at: 310 310
Size: 4546
DrdI GACnn_nn'nnGTC
Cuts at: 1108 2250 3110 1108
Size: 1142 860 2544
Fragments arranged by size:
2544 1142 860
EaeI y'GGCC_r
Cuts at: 1367 2129 2303 2694 2721 4068 1367
Size: 762 174 391 27 1347 1845
Fragments arranged by size:
1845 1347 762 391 174 27
EagI C'GGCC_G
Cuts at: 2129 2129
Size: 4546
EarI CTCTTCn'nnn_
Cuts at: 2567 2777 2567
Size: 210 4336
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1002 2752 3064 3210 4356 1002
Size: 1750 312 146 1146 1192
Fragments arranged by size:
1750 1192 1146 312 146
EcoICRI GAG'CTC
Cuts at: 747 1556 747
Size: 809 3737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 1185 1289 2369 2801 3550 3818 953
Size: 232 104 1080 432 749 268 1681
Fragments arranged by size:
1681 1080 749 432 268 232 104
EcoO109I rG'GnC_Cy
Cuts at: 1683 1683
Size: 4546
EcoRI G'AATT_C
Cuts at: 399 542 1548 399
Size: 143 1006 3397
Fragments arranged by size:
3397 1006 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 500 1294 2563 500
Size: 794 1269 2483
Fragments arranged by size:
2483 1269 794
FspI TGC'GCA
Cuts at: 2325 2325
Size: 4546
HaeII r_GCGC'y
Cuts at: 640 1978 2226 3250 4229 640
Size: 1338 248 1024 979 957
Fragments arranged by size:
1338 1024 979 957 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1207 1239 2386 2416 2418 2448 1207
Size: 32 1147 30 2 30 3305
Fragments arranged by size:
3305 1147 32 30 30 2
HincII GTy'rAC
Cuts at: 507 1583 507
Size: 1076 3470
HindIII A'AGCT_T
Cuts at: 1479 1479
Size: 4546
KasI G'GCGC_C
Cuts at: 1974 2222 1974
Size: 248 4298
KpnI G_GTAC'C
Cuts at: 366 769 1162 1564 366
Size: 403 393 402 3348
Fragments arranged by size:
3348 403 402 393
MluI A'CGCG_T
Cuts at: 3979 3979
Size: 4546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 435 1058 3217 3401 435
Size: 623 2159 184 1580
Fragments arranged by size:
2159 1580 623 184
MscI TGG'CCA
Cuts at: 2305 2305
Size: 4546
MslI CAynn'nnrTG
Cuts at: 372 577 2533 2660 372
Size: 205 1956 127 2258
Fragments arranged by size:
2258 1956 205 127
MspA1I CmG'CkG
Cuts at: 724 894 1655 1969 2329 3344 3589 724
Size: 170 761 314 360 1015 245 1681
Fragments arranged by size:
1681 1015 761 360 314 245 170
NaeI GCC'GGC
Cuts at: 2725 2725
Size: 4546
NarI GG'CG_CC
Cuts at: 1975 2223 1975
Size: 248 4298
NgoMIV G'CCGG_C
Cuts at: 2723 2723
Size: 4546
NruI TCG'CGA
Cuts at: 2907 2907
Size: 4546
NspI r_CATG'y
Cuts at: 590 1198 1597 2097 3006 590
Size: 608 399 500 909 2130
Fragments arranged by size:
2130 909 608 500 399
PciI A'CATG_T
Cuts at: 3002 3002
Size: 4546
PfoI T'CCnGG_A
Cuts at: 1112 1112
Size: 4546
PmeI GTTT'AAAC
Cuts at: 1543 1543
Size: 4546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3685 3717 3685
Size: 32 4514
PshAI GACnn'nnGTC
Cuts at: 323 1355 323
Size: 1032 3514
PstI C_TGCA'G
Cuts at: 1350 1591 2276 1350
Size: 241 685 3620
Fragments arranged by size:
3620 685 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4546
PvuII CAG'CTG
Cuts at: 1969 2329 1969
Size: 360 4186
RsrII CG'GwC_CG
Cuts at: 2739 2739
Size: 4546
SacI G_AGCT'C
Cuts at: 749 1558 749
Size: 809 3737
SalI G'TCGA_C
Cuts at: 1581 1581
Size: 4546
SapI GCTCTTCn'nnn_
Cuts at: 2567 2777 2567
Size: 210 4336
SbfI CC_TGCA'GG
Cuts at: 1591 1591
Size: 4546
ScaI AGT'ACT
Cuts at: 4063 4063
Size: 4546
SfcI C'TryA_G
Cuts at: 33 1346 1587 2272 3267 3458 33
Size: 1313 241 685 995 191 1121
Fragments arranged by size:
1313 1121 995 685 241 191
SfoI GGC'GCC
Cuts at: 1976 2224 1976
Size: 248 4298
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4546
SmaI CCC'GGG
Cuts at: 841 1566 841
Size: 725 3821
SmlI C'TyrA_G
Cuts at: 1003 1699 2614 3108 3370 1003
Size: 696 915 494 262 2179
Fragments arranged by size:
2179 915 696 494 262
SnaBI TAC'GTA
Cuts at: 1536 1536
Size: 4546
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4546
SphI G_CATG'C
Cuts at: 1597 2097 1597
Size: 500 4046
StuI AGG'CCT
Cuts at: 1525 1525
Size: 4546
StyI C'CwwG_G
Cuts at: 1678 2032 1678
Size: 354 4192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2127 2260 2719 2930 2127
Size: 133 459 211 3743
Fragments arranged by size:
3743 459 211 133
TatI w'GTAC_w
Cuts at: 4061 4061
Size: 4546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4063 4095 4063
Size: 32 4514
Tth111I GACn'n_nGTC
Cuts at: 2341 2341
Size: 4546
XbaI T'CTAG_A
Cuts at: 960 1575 960
Size: 615 3931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2039 2039
Size: 4546
XmaI C'CCGG_G
Cuts at: 839 1564 839
Size: 725 3821
XmnI GAAnn'nnTTC
Cuts at: 794 794
Size: 4546
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NruI NspI PciI PfoI PmeI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NsiI PacI PasI
PflMI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI
ZraI