Restriction map for
pF1KSDB0040
(Circular) (Six-base) MAPSORT of: pF1KSDB0040.seq Check: 4066 from: 1 to: 4999
pF1KSDB0040 4999 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 120 773 1393 1413 1667 120
Size: 653 620 20 254 3452
Fragments arranged by size:
3452 653 620 254 20
AatII G_ACGT'C
Cuts at: 925 925
Size: 4999
AccI GT'mk_AC
Cuts at: 665 2035 665
Size: 1370 3629
Acc65I G'GTAC_C
Cuts at: 2013 2013
Size: 4999
AclI AA'CG_TT
Cuts at: 4839 4839
Size: 4999
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 669 2822 3254 4003 4271 538
Size: 131 2153 432 749 268 1266
Fragments arranged by size:
2153 1266 749 432 268 131
AfeI AGC'GCT
Cuts at: 307 307
Size: 4999
AflIII A'CryG_T
Cuts at: 608 2349 3455 4432 608
Size: 1741 1106 977 1175
Fragments arranged by size:
1741 1175 1106 977
AgeI A'CCGG_T
Cuts at: 3387 3387
Size: 4999
AhdI GACnn_n'nnGTC
Cuts at: 497 850 497
Size: 353 4646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 160 160
Size: 4999
AlwNI CAG_nnn'CTG
Cuts at: 621 2302 3871 621
Size: 1681 1569 1749
Fragments arranged by size:
1749 1681 1569
ApaLI G'TGCA_C
Cuts at: 3769 3769
Size: 4999
ApoI r'AATT_y
Cuts at: 2001 4352 4608 2001
Size: 2351 256 2392
Fragments arranged by size:
2392 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4999
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4999
AvaI C'yCGr_G
Cuts at: 322 1321 2017 322
Size: 999 696 3304
Fragments arranged by size:
3304 999 696
AvrII C'CTAG_G
Cuts at: 357 357
Size: 4999
BamHI G'GATC_C
Cuts at: 2022 2022
Size: 4999
BanI G'GyrC_C
Cuts at: 463 794 1271 2013 2427 2675 2710 463
Size: 331 477 742 414 248 35 2752
Fragments arranged by size:
2752 742 477 414 331 248 35
BanII G_rGCy'C
Cuts at: 223 431 461 1039 1109 2011 3041 223
Size: 208 30 578 70 902 1030 2181
Fragments arranged by size:
2181 1030 902 578 208 70 30
BbeI G_GCGC'C
Cuts at: 467 1275 2431 2679 467
Size: 808 1156 248 2787
Fragments arranged by size:
2787 1156 808 248
BbsI GAAGACnn'nnnn_
Cuts at: 531 1179 1545 531
Size: 648 366 3985
Fragments arranged by size:
3985 648 366
BbvCI CC'TCA_GC
Cuts at: 550 550
Size: 4999
BciVI GTATCCnnnnn_n'
Cuts at: 2895 3658 4999 2895
Size: 763 1341 2895
Fragments arranged by size:
2895 1341 763
BclI T'GATC_A
Cuts at: 2517 2517
Size: 4999
BglI GCCn_nnn'nGGC
Cuts at: 908 1686 908
Size: 778 4221
BglII A'GATC_T
Cuts at: 860 2512 4551 860
Size: 1652 2039 1308
Fragments arranged by size:
2039 1652 1308
BlpI GC'TnA_GC
Cuts at: 2109 2109
Size: 4999
Bme1580I G_kGCm'C
Cuts at: 962 1922 2622 2715 3773 962
Size: 960 700 93 1058 2188
Fragments arranged by size:
2188 1058 960 700 93
BmrI ACTGGGnnnn_n'
Cuts at: 2209 2290 2625 4771 2209
Size: 81 335 2146 2437
Fragments arranged by size:
2437 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3364 3364
Size: 4999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 445 661 724 739 1195 1228 1591 1890
Size: 9 216 63 15 456 33 363 299
Cuts at: 1890 436
Size: 3545
Fragments arranged by size:
3545 456 363 299 216 63 33 15
9
Bpu10I CC'TnA_GC
Cuts at: 550 550
Size: 4999
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 832 2173 3052 3546 3844 616
Size: 216 1341 879 494 298 1771
Fragments arranged by size:
1771 1341 879 494 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 505 1527 4703 505
Size: 1022 3176 801
Fragments arranged by size:
3176 1022 801
BsaAI yAC'GTr
Cuts at: 1310 1607 1989 2350 1310
Size: 297 382 361 3959
Fragments arranged by size:
3959 382 361 297
BsaBI GATnn'nnATC
Cuts at: 6 592 2511 4550 6
Size: 586 1919 2039 455
Fragments arranged by size:
2039 1919 586 455
BsaHI Gr'CG_yC
Cuts at: 464 536 922 1272 1835 2428 2676 3380 4876
Size: 72 386 350 563 593 248 704 1496
Cuts at: 4876 464
Size: 587
Fragments arranged by size:
1496 704 593 587 563 386 350 248
72
BsaWI w'CCGG_w
Cuts at: 488 2186 2707 3387 3661 3808 4219 488
Size: 1698 521 680 274 147 411 1268
Fragments arranged by size:
1698 1268 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 721 751 721
Size: 30 4969
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 177 433 177
Size: 256 4743
BseYI C'CCAG_C
Cuts at: 172 216 495 1418 2422 3180 3759 4255 172
Size: 44 279 923 1004 758 579 496 916
Fragments arranged by size:
1004 923 916 758 579 496 279 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1471 751
Size: 720 4279
BsiEI CG_ry'CG
Cuts at: 104 2539 2585 3795 104
Size: 2435 46 1210 1308
Fragments arranged by size:
2435 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 271 431 1145 2011 2789 2979 3773 271
Size: 160 714 866 778 190 794 1497
Fragments arranged by size:
1497 866 794 778 714 190 160
BsmBI CGTCTCn'nnnn_
Cuts at: 334 334
Size: 4999
Bsp1286I G_dGCh'C
Cuts at: 223 271 431 461 962 1039 1109 1145 1922
Size: 48 160 30 501 77 70 36 777
Cuts at: 1922 2011 2622 2715 2789 2979 3041 3773 223
Size: 89 611 93 74 190 62 732 1449
Fragments arranged by size:
1449 777 732 611 501 190 160 93
89 77 74 70 62 48 36 30
BspEI T'CCGG_A
Cuts at: 488 4219 488
Size: 3731 1268
BspHI T'CATG_A
Cuts at: 4175 4175
Size: 4999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 120 773 1142 1393 1413 1667 2047 2261 2563
Size: 653 369 251 20 254 380 214 302
Cuts at: 2563 2944 120
Size: 381 2175
Fragments arranged by size:
2175 653 381 380 369 302 254 251
214 20
BsrBI CCG'CTC
Cuts at: 50 3289 3343 4829 4994 50
Size: 3239 54 1486 165 55
Fragments arranged by size:
3239 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1542 2909 1542
Size: 1367 3632
BsrFI r'CCGG_y
Cuts at: 1295 2995 3176 3387 1295
Size: 1700 181 211 2907
Fragments arranged by size:
2907 1700 211 181
BsrGI T'GTAC_A
Cuts at: 623 623
Size: 4999
BssSI C'ACGA_G
Cuts at: 3268 3628 3268
Size: 360 4639
BstAPI GCAn_nnn'nTGC
Cuts at: 1053 2374 1053
Size: 1321 3678
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4999
BstXI CCAn_nnnn'nTGG
Cuts at: 1812 1812
Size: 4999
BstYI r'GATC_y
Cuts at: 1 860 1070 2022 2489 2512 2847 3093 4107
Size: 859 210 952 467 23 335 246 1014
Cuts at: 4107 4193 4205 4551 1
Size: 86 12 346 449
Fragments arranged by size:
1014 952 859 467 449 346 335 246
210 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 784 784
Size: 4999
BtgI C'CryG_G
Cuts at: 242 452 1097 1119 1811 242
Size: 210 645 22 692 3430
Fragments arranged by size:
3430 692 645 210 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1602 1785 2947 3128 3291 1602
Size: 183 1162 181 163 3310
Fragments arranged by size:
3310 1162 183 181 163
BtsI GCAGTG_nn'
Cuts at: 939 2240 939
Size: 1301 3698
DraI TTT'AAA
Cuts at: 1996 1996
Size: 4999
DraIII CAC_nnn'GTG
Cuts at: 1197 1449 1197
Size: 252 4747
DrdI GACnn_nn'nnGTC
Cuts at: 927 2703 3563 927
Size: 1776 860 2363
Fragments arranged by size:
2363 1776 860
EaeI y'GGCC_r
Cuts at: 239 968 1094 1622 2582 2756 3147 3174 4521
Size: 729 126 528 960 174 391 27 1347
Cuts at: 4521 239
Size: 717
Fragments arranged by size:
1347 960 729 717 528 391 174 126
27
EagI C'GGCC_G
Cuts at: 2582 2582
Size: 4999
EarI CTCTTCn'nnn_
Cuts at: 484 1849 3020 3230 484
Size: 1365 1171 210 2253
Fragments arranged by size:
2253 1365 1171 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 261 964 1938 3205 3517 3663 4809 261
Size: 703 974 1267 312 146 1146 451
Fragments arranged by size:
1267 1146 974 703 451 312 146
EcoICRI GAG'CTC
Cuts at: 429 2009 429
Size: 1580 3419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 445 538 661 669 724 739 1195 1228
Size: 9 93 123 8 55 15 456 33
Cuts at: 1228 1591 1890 2822 3254 4003 4271 436
Size: 363 299 932 432 749 268 1164
Fragments arranged by size:
1164 932 749 456 432 363 299 268
123 93 55 33 15 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 831 831
Size: 4999
EcoO109I rG'GnC_Cy
Cuts at: 618 2136 618
Size: 1518 3481
EcoRI G'AATT_C
Cuts at: 2001 2001
Size: 4999
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4999
FspI TGC'GCA
Cuts at: 1669 2778 1669
Size: 1109 3890
HaeII r_GCGC'y
Cuts at: 309 452 467 757 1275 2431 2679 3703 4682
Size: 143 15 290 518 1156 248 1024 979
Cuts at: 4682 309
Size: 626
Fragments arranged by size:
1156 1024 979 626 518 290 248 143
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 483 515 742 774 836 868 2839 2869 2871
Size: 32 227 32 62 32 1971 30 2
Cuts at: 2871 2901 483
Size: 30 2581
Fragments arranged by size:
2581 1971 227 62 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2036 2036
Size: 4999
KasI G'GCGC_C
Cuts at: 463 1271 2427 2675 463
Size: 808 1156 248 2787
Fragments arranged by size:
2787 1156 808 248
KpnI G_GTAC'C
Cuts at: 2017 2017
Size: 4999
MluI A'CGCG_T
Cuts at: 4432 4432
Size: 4999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1748 1910 3670 3854 1748
Size: 162 1760 184 2893
Fragments arranged by size:
2893 1760 184 162
MscI TGG'CCA
Cuts at: 2758 2758
Size: 4999
MslI CAynn'nnrTG
Cuts at: 130 1661 1810 1978 2986 3113 130
Size: 1531 149 168 1008 127 2016
Fragments arranged by size:
2016 1531 1008 168 149 127
MspA1I CmG'CkG
Cuts at: 260 689 711 955 1077 1092 1121 1164 1236
Size: 429 22 244 122 15 29 43 72
Cuts at: 1236 1277 2108 2422 2782 3797 4042 260
Size: 41 831 314 360 1015 245 1217
Fragments arranged by size:
1217 1015 831 429 360 314 245 244
122 72 43 41 29 22 15
NaeI GCC'GGC
Cuts at: 3178 3178
Size: 4999
NarI GG'CG_CC
Cuts at: 464 1272 2428 2676 464
Size: 808 1156 248 2787
Fragments arranged by size:
2787 1156 808 248
NcoI C'CATG_G
Cuts at: 1811 1811
Size: 4999
NgoMIV G'CCGG_C
Cuts at: 3176 3176
Size: 4999
NruI TCG'CGA
Cuts at: 3360 3360
Size: 4999
NspI r_CATG'y
Cuts at: 171 612 1666 2050 2550 3459 171
Size: 441 1054 384 500 909 1711
Fragments arranged by size:
1711 1054 909 500 441 384
PciI A'CATG_T
Cuts at: 608 3455 608
Size: 2847 2152
PflMI CCAn_nnn'nTGG
Cuts at: 1080 1197 1496 1080
Size: 117 299 4583
Fragments arranged by size:
4583 299 117
PfoI T'CCnGG_A
Cuts at: 815 1220 1403 815
Size: 405 183 4411
Fragments arranged by size:
4411 405 183
PmeI GTTT'AAAC
Cuts at: 1996 1996
Size: 4999
PmlI CAC'GTG
Cuts at: 1310 1310
Size: 4999
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4138 4170 4138
Size: 32 4967
PpuMI rG'GwC_Cy
Cuts at: 618 618
Size: 4999
PstI C_TGCA'G
Cuts at: 1067 2044 2729 1067
Size: 977 685 3337
Fragments arranged by size:
3337 977 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4999
PvuII CAG'CTG
Cuts at: 711 1077 1092 1164 2422 2782 711
Size: 366 15 72 1258 360 2928
Fragments arranged by size:
2928 1258 366 360 72 15
RsrII CG'GwC_CG
Cuts at: 3192 3192
Size: 4999
SacI G_AGCT'C
Cuts at: 431 2011 431
Size: 1580 3419
SacII CC_GC'GG
Cuts at: 1122 1122
Size: 4999
SalI G'TCGA_C
Cuts at: 2034 2034
Size: 4999
SapI GCTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4789
SbfI CC_TGCA'GG
Cuts at: 2044 2044
Size: 4999
ScaI AGT'ACT
Cuts at: 4516 4516
Size: 4999
SexAI A'CCwGG_T
Cuts at: 1517 1517
Size: 4999
SfcI C'TryA_G
Cuts at: 33 1063 2040 2725 3720 3911 33
Size: 1030 977 685 995 191 1121
Fragments arranged by size:
1121 1030 995 977 685 191
SfoI GGC'GCC
Cuts at: 465 1273 2429 2677 465
Size: 808 1156 248 2787
Fragments arranged by size:
2787 1156 808 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4999
SmaI CCC'GGG
Cuts at: 2019 2019
Size: 4999
SmlI C'TyrA_G
Cuts at: 631 847 2152 3067 3561 3823 631
Size: 216 1305 915 494 262 1807
Fragments arranged by size:
1807 1305 915 494 262 216
SnaBI TAC'GTA
Cuts at: 1989 1989
Size: 4999
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4999
SphI G_CATG'C
Cuts at: 171 2050 2550 171
Size: 1879 500 2620
Fragments arranged by size:
2620 1879 500
StyI C'CwwG_G
Cuts at: 357 1811 2131 2485 357
Size: 1454 320 354 2871
Fragments arranged by size:
2871 1454 354 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 909 1251 2580 2713 3172 3383 909
Size: 342 1329 133 459 211 2525
Fragments arranged by size:
2525 1329 459 342 211 133
TatI w'GTAC_w
Cuts at: 623 4514 623
Size: 3891 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4516 4548 4516
Size: 32 4967
Tth111I GACn'n_nGTC
Cuts at: 661 2794 661
Size: 2133 2866
XbaI T'CTAG_A
Cuts at: 2028 2028
Size: 4999
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 180 207 2492 180
Size: 27 2285 2687
Fragments arranged by size:
2687 2285 27
XmaI C'CCGG_G
Cuts at: 2017 2017
Size: 4999
ZraI GAC'GTC
Cuts at: 923 923
Size: 4999
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AflII AleI AloI ApaI AscI BaeI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PasI PshAI PsiI PspOMI
PspXI PsrI SanDI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI