Restriction map for
pF1KSDB0041
(Circular) (Six-base) MAPSORT of: pF1KSDB0041.seq Check: 4334 from: 1 to: 4528
pF1KSDB0041 4528 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1564 1564
Size: 4528
Acc65I G'GTAC_C
Cuts at: 586 1411 1542 586
Size: 825 131 3572
Fragments arranged by size:
3572 825 131
AclI AA'CG_TT
Cuts at: 4368 4368
Size: 4528
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 905 1304 2351 2783 3532 3800 863
Size: 42 399 1047 432 749 268 1591
Fragments arranged by size:
1591 1047 749 432 399 268 42
AflIII A'CryG_T
Cuts at: 1878 2984 3961 1878
Size: 1106 977 2445
Fragments arranged by size:
2445 1106 977
AgeI A'CCGG_T
Cuts at: 2916 2916
Size: 4528
AhdI GACnn_n'nnGTC
Cuts at: 281 281
Size: 4528
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 4528
AlwNI CAG_nnn'CTG
Cuts at: 757 1831 3400 757
Size: 1074 1569 1885
Fragments arranged by size:
1885 1569 1074
ApaLI G'TGCA_C
Cuts at: 3298 3298
Size: 4528
ApoI r'AATT_y
Cuts at: 997 1530 3881 4137 997
Size: 533 2351 256 1388
Fragments arranged by size:
2351 1388 533 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4528
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4528
AvaI C'yCGr_G
Cuts at: 296 1427 1546 296
Size: 1131 119 3278
Fragments arranged by size:
3278 1131 119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 570 603 570
Size: 33 4495
BamHI G'GATC_C
Cuts at: 1551 1551
Size: 4528
BanI G'GyrC_C
Cuts at: 136 586 1411 1542 1956 2204 2239 136
Size: 450 825 131 414 248 35 2425
Fragments arranged by size:
2425 825 450 414 248 131 35
BanII G_rGCy'C
Cuts at: 1540 2570 1540
Size: 1030 3498
BbeI G_GCGC'C
Cuts at: 1960 2208 1960
Size: 248 4280
BbsI GAAGACnn'nnnn_
Cuts at: 272 272
Size: 4528
BbvCI CC'TCA_GC
Cuts at: 732 732
Size: 4528
BciVI GTATCCnnnnn_n'
Cuts at: 624 1342 2424 3187 4528 624
Size: 718 1082 763 1341 624
Fragments arranged by size:
1341 1082 763 718 624
BclI T'GATC_A
Cuts at: 2046 2046
Size: 4528
BglII A'GATC_T
Cuts at: 637 2041 4080 637
Size: 1404 2039 1085
Fragments arranged by size:
2039 1404 1085
BlpI GC'TnA_GC
Cuts at: 1638 1638
Size: 4528
Bme1580I G_kGCm'C
Cuts at: 139 2151 2244 3302 139
Size: 2012 93 1058 1365
Fragments arranged by size:
2012 1365 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1738 1819 2154 4300 1738
Size: 81 335 2146 1966
Fragments arranged by size:
2146 1966 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 4528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 893 734
Size: 159 4369
Bpu10I CC'TnA_GC
Cuts at: 732 732
Size: 4528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 644 654 1120 1702 2581 3075 3373 374
Size: 270 10 466 582 879 494 298 1529
Fragments arranged by size:
1529 879 582 494 466 298 270 10
BsaI GGTCTCn'nnnn_
Cuts at: 1263 4232 1263
Size: 2969 1559
BsaAI yAC'GTr
Cuts at: 1518 1879 1518
Size: 361 4167
BsaBI GATnn'nnATC
Cuts at: 6 2040 4079 6
Size: 2034 2039 455
Fragments arranged by size:
2039 2034 455
BsaHI Gr'CG_yC
Cuts at: 1957 2205 2909 4405 1957
Size: 248 704 1496 2080
Fragments arranged by size:
2080 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1715 2236 2916 3190 3337 3748 1715
Size: 521 680 274 147 411 2495
Fragments arranged by size:
2495 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 756 786 924 954 756
Size: 30 138 30 4330
Fragments arranged by size:
4330 138 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1376 1466 1376
Size: 90 4438
BseYI C'CCAG_C
Cuts at: 396 1951 2709 3288 3784 396
Size: 1555 758 579 496 1140
Fragments arranged by size:
1555 1140 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 742
Size: 4528
BsiEI CG_ry'CG
Cuts at: 104 2068 2114 3324 104
Size: 1964 46 1210 1308
Fragments arranged by size:
1964 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 833 918 1540 2318 2508 3302 833
Size: 85 622 778 190 794 2059
Fragments arranged by size:
2059 794 778 622 190 85
Bsp1286I G_dGCh'C
Cuts at: 139 833 918 1540 2151 2244 2318 2508 2570
Size: 694 85 622 611 93 74 190 62
Cuts at: 2570 3302 139
Size: 732 1365
Fragments arranged by size:
1365 732 694 622 611 190 93 85
74 62
BspEI T'CCGG_A
Cuts at: 3748 3748
Size: 4528
BspHI T'CATG_A
Cuts at: 3704 3704
Size: 4528
BspMI ACCTGCnnnn'nnnn_
Cuts at: 753 1576 1790 2092 2473 753
Size: 823 214 302 381 2808
Fragments arranged by size:
2808 823 381 302 214
BsrBI CCG'CTC
Cuts at: 50 131 2818 2872 4358 4523 50
Size: 81 2687 54 1486 165 55
Fragments arranged by size:
2687 1486 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 2438 2438
Size: 4528
BsrFI r'CCGG_y
Cuts at: 2524 2705 2916 2524
Size: 181 211 4136
Fragments arranged by size:
4136 211 181
BssSI C'ACGA_G
Cuts at: 2797 3157 2797
Size: 360 4168
BstAPI GCAn_nnn'nTGC
Cuts at: 1903 1903
Size: 4528
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4528
BstYI r'GATC_y
Cuts at: 1 182 637 1188 1551 2018 2041 2376 2622
Size: 181 455 551 363 467 23 335 246
Cuts at: 2622 3636 3722 3734 4080 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 551 467 455 449 363 346 335
246 181 86 23 12
BtgI C'CryG_G
Cuts at: 327 446 707 1335 1341 1377 327
Size: 119 261 628 6 36 3478
Fragments arranged by size:
3478 628 261 119 36 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2476 2657 2820 2476
Size: 181 163 4184
Fragments arranged by size:
4184 181 163
BtsI GCAGTG_nn'
Cuts at: 725 1418 1769 725
Size: 693 351 3484
Fragments arranged by size:
3484 693 351
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1488 1523 1488
Size: 35 4493
DraI TTT'AAA
Cuts at: 1525 1525
Size: 4528
DrdI GACnn_nn'nnGTC
Cuts at: 2232 3092 2232
Size: 860 3668
EaeI y'GGCC_r
Cuts at: 1338 2111 2285 2676 2703 4050 1338
Size: 773 174 391 27 1347 1816
Fragments arranged by size:
1816 1347 773 391 174 27
EagI C'GGCC_G
Cuts at: 2111 2111
Size: 4528
EarI CTCTTCn'nnn_
Cuts at: 1043 2549 2759 1043
Size: 1506 210 2812
Fragments arranged by size:
2812 1506 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 193 2734 3046 3192 4338 193
Size: 2541 312 146 1146 383
Fragments arranged by size:
2541 1146 383 312 146
EcoICRI GAG'CTC
Cuts at: 1538 1538
Size: 4528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 863 893 905 1304 2351 2783 3532 3800
Size: 129 30 12 399 1047 432 749 268
Cuts at: 3800 734
Size: 1462
Fragments arranged by size:
1462 1047 749 432 399 268 129 30
12
EcoO109I rG'GnC_Cy
Cuts at: 1665 1665
Size: 4528
EcoRI G'AATT_C
Cuts at: 1530 1530
Size: 4528
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1114 1210 2545 1114
Size: 96 1335 3097
Fragments arranged by size:
3097 1335 96
FspI TGC'GCA
Cuts at: 2307 2307
Size: 4528
HaeII r_GCGC'y
Cuts at: 1960 2208 3232 4211 1960
Size: 248 1024 979 2277
Fragments arranged by size:
2277 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 290 299 322 622 654 756 788 2368
Size: 23 9 23 300 32 102 32 1580
Cuts at: 2368 2398 2400 2430 267
Size: 30 2 30 2365
Fragments arranged by size:
2365 1580 300 102 32 32 30 30
23 23 9 2
HincII GTy'rAC
Cuts at: 1565 1565
Size: 4528
KasI G'GCGC_C
Cuts at: 1956 2204 1956
Size: 248 4280
KpnI G_GTAC'C
Cuts at: 590 1415 1546 590
Size: 825 131 3572
Fragments arranged by size:
3572 825 131
MluI A'CGCG_T
Cuts at: 3961 3961
Size: 4528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3199 3383 3199
Size: 184 4344
MscI TGG'CCA
Cuts at: 2287 2287
Size: 4528
MslI CAynn'nnrTG
Cuts at: 953 2515 2642 953
Size: 1562 127 2839
Fragments arranged by size:
2839 1562 127
MspA1I CmG'CkG
Cuts at: 757 1637 1951 2311 3326 3571 757
Size: 880 314 360 1015 245 1714
Fragments arranged by size:
1714 1015 880 360 314 245
NaeI GCC'GGC
Cuts at: 2707 2707
Size: 4528
NarI GG'CG_CC
Cuts at: 1957 2205 1957
Size: 248 4280
NcoI C'CATG_G
Cuts at: 446 707 446
Size: 261 4267
NgoMIV G'CCGG_C
Cuts at: 2705 2705
Size: 4528
NruI TCG'CGA
Cuts at: 2889 2889
Size: 4528
NspI r_CATG'y
Cuts at: 1579 2079 2988 1579
Size: 500 909 3119
Fragments arranged by size:
3119 909 500
PciI A'CATG_T
Cuts at: 2984 2984
Size: 4528
PflMI CCAn_nnn'nTGG
Cuts at: 447 1336 447
Size: 889 3639
PmeI GTTT'AAAC
Cuts at: 1525 1525
Size: 4528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3667 3699 3667
Size: 32 4496
PspXI vC'TCGA_Gb
Cuts at: 1427 1427
Size: 4528
PstI C_TGCA'G
Cuts at: 1573 2258 1573
Size: 685 3843
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4528
PvuII CAG'CTG
Cuts at: 757 1951 2311 757
Size: 1194 360 2974
Fragments arranged by size:
2974 1194 360
RsrII CG'GwC_CG
Cuts at: 2721 2721
Size: 4528
SacI G_AGCT'C
Cuts at: 1540 1540
Size: 4528
SalI G'TCGA_C
Cuts at: 1563 1563
Size: 4528
SapI GCTCTTCn'nnn_
Cuts at: 2549 2759 2549
Size: 210 4318
SbfI CC_TGCA'GG
Cuts at: 1573 1573
Size: 4528
ScaI AGT'ACT
Cuts at: 4045 4045
Size: 4528
SfcI C'TryA_G
Cuts at: 33 1569 2254 3249 3440 33
Size: 1536 685 995 191 1121
Fragments arranged by size:
1536 1121 995 685 191
SfoI GGC'GCC
Cuts at: 1958 2206 1958
Size: 248 4280
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4528
SmaI CCC'GGG
Cuts at: 1548 1548
Size: 4528
SmlI C'TyrA_G
Cuts at: 296 389 659 669 1099 1427 1681 2596 3090
Size: 93 270 10 430 328 254 915 494
Cuts at: 3090 3352 296
Size: 262 1472
Fragments arranged by size:
1472 915 494 430 328 270 262 254
93 10
SnaBI TAC'GTA
Cuts at: 1518 1518
Size: 4528
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4528
SphI G_CATG'C
Cuts at: 1579 2079 1579
Size: 500 4028
SspI AAT'ATT
Cuts at: 1242 1242
Size: 4528
StuI AGG'CCT
Cuts at: 789 789
Size: 4528
StyI C'CwwG_G
Cuts at: 173 446 707 928 1660 2014 173
Size: 273 261 221 732 354 2687
Fragments arranged by size:
2687 732 354 273 261 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 288 2109 2242 2701 2912 288
Size: 1821 133 459 211 1904
Fragments arranged by size:
1904 1821 459 211 133
TatI w'GTAC_w
Cuts at: 846 4043 846
Size: 3197 1331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 924 956 4045 4077 924
Size: 32 3089 32 1375
Fragments arranged by size:
3089 1375 32 32
Tth111I GACn'n_nGTC
Cuts at: 2323 2323
Size: 4528
XbaI T'CTAG_A
Cuts at: 1557 1557
Size: 4528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2021 2021
Size: 4528
XhoI C'TCGA_G
Cuts at: 296 1427 296
Size: 1131 3397
XmaI C'CCGG_G
Cuts at: 1546 1546
Size: 4528
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PflMI PmeI
PpiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI XmnI ZraI